PAN FACILITY
Beckman Center B065
275 Campus Drive, West
Stanford University 94305
(650)723-3189
Date
ORD#
REAL-TIME (QPCR) REQUEST FORM
Requestor Name:
PI Name:
Phone:
Concentration Guidelines
Email:
Account Type:
On Campus
Off Campus
PTA / PO #
Service
Reverse Transcription (RT)
Absolute Quantification
Relative Quantification
Comparative ∆CT
Genotyping
Number of Assays
Sample Type
Human
Mouse
Rat
Yeast
Other____________
RNA
cDNA
gDNA
Other__________
Name of Endogenous Control/HKG
Name(s) of Samples
DNA/cDNA
10ng/ul
Primers
10uM
TaqMan
SYBR Green
Name of Reference Sample
1.
2ug-5ug
Detection Method
Number of Samples
(Genes of Interest – GOI)
Name(s) of Assay/GOI
Sample Source
RNA
1.
2.
2.
3.
3.
4.
4.
5.
5.
6.
6.
7.
7.
8.
8.
Please fill out the plate format below.
Samples are run in triplicate in a 96-well format. Due to plate format, a limited number of assays and
samples can be run together on a plate. Excess samples or assays will be run on additional plates, but
please remember 3 wells must be reserved for endogenous control and reference sample.
1
A
B
C
D
E
F
G
H
2
3
HKG + reference sample
4
5
6
7
8
9
10
11
12