Dr Li-Jun Huo
Professor in Animal reproduction
Expertise Statement
Teaching Duties

Animal reproductive physiology, Animal Reproduction, and Animal
Reproduction and Technology
Research Interests

Molecular regulation of folliculogenesis, ovulation control, oocyte meiotic
maturation in mammals

Roles and regulation of microRNA and SUMOylation during mouse
folliculogenesis

Spermatogenesis and fertility control in farm animal
Professional Memberships

Member of Society of Reproductive Biology in China

Member of Society of Cell and Molecular Microscopic Technology in China
Education & Working Experience

1995-1999: Northeast Agricultural University, Bachelor degree

1999-2002: Northeast Agricultural University, Master degree

2002-2005: State Key Laboratory of Reproductive Biology, Institute of
Zoology, Chinese Academy of Sciences, Doctor degree

2005-2008: Stowers Institute for Medical Research, postdoc training

2008-
: College of Animal Science and Technology, Huazhong
Agricultural University,
Professor
Publications
1. Sang L, Yang WC, Han L, Liang AX, Hua GH, Xiong JJ, Huo LJ, Yang LG. An
immunological method to screen sex-specific proteins of bovine sperm. J Dairy
Sci. 2011 Apr;94(4):2060-70.
2. Bai LY, Liang AX, Zhang J, Yang FF, Han L, Huo LJ, Yang LG. Effects of
immunization against a DNA vaccine encoding somatostatin gene (pGM-CSF/SS)
by attenuated Salmonella typhimurium on growth, reproduction and lactation in
female mice. Theriogenology. 2011 Jan 1;75(1):155-63.
3. Fan H-Y, Huo L-J, Chen D-Y, Schatten H, Sun Q-Y. Protein kinase C and
mitogen-activated protein kinase cascade in mouse cumulus cells: cross talk and
effect on meiotic resumption of oocyte. Biol Reprod 2004; 70:1178-87.
4. Fan HY, Huo LJ, Meng XQ, Zhong ZS, Hou Y, Chen DY, Sun QY. Involvement of
calcium/calmodulin-dependent protein kinase II (CaMKII) in meiotic maturation
and activation of pig oocytes. Biol Reprod 2003; 69:1552-64.
5. Fan HY, Huo LJ, Sun QY. Roles of calmodulin-dependent protein kinase II in
meiotic maturation and fertilization of oocytes. Progress in Biochemistry and
Biophysics 2003; 30:171-4.
6. Fan, HY, Meng XQ, Huo LJ, Tong C, Chen DY, Sun QY. Methodology for detection
of spindle-associated proteins by confocal microscopy in mammalian oocytes.
Progress in Biochemistry and Biophysics. 2004; 31(1):54-8.
7. Huo LJ, Fan HY, Chen DY, Sun QY. Function and interaction of
maturation-promoting factor and mitogen-activated protein kinase during
meiotic maturation and fertilization of oocyte. Progress in Natural Science 2004;
14:562-7.
8. Huo L-J, Fan H-Y, Liang C-G, Yu L-Z, Zhong Z-S, Chen D-Y, Sun Q-Y. Regulation of
ubiquitin-proteasome pathway on pig oocyte meiotic maturation and
fertilization. Biol Reprod 2004; 71:853-62.
9. Huo
L-J,
Fan
H-Y,
Zhong
Z-S,
Chen
D-Y,
Schatten
H,
Sun
Q-Y.
Ubiquitin-proteasome pathway modulates mouse oocyte meiotic maturation
and fertilization via regulation of MAPK cascade and cyclin B1 degradation.
Mech Dev 2004; 121:1275-87.
10. Huo L-J, Liang C-G, Yu L-Z, Zhong Z-S, Yang Z-M, Fan H-Y, Chen D-Y, Sun Q-Y.
Inducible nitric oxide synthase-derived nitric oxide regulates germinal vesicle
breakdown and first polar body emission in the mouse oocyte. Reproduction
2005; 129:403-9.
11. Huo L-J, Ma X-H, Yang Z-M. Assessment of sperm viability, mitochondrial activity,
capacitation and acrosome intactness in extended boar semen during long-term
storage. Theriogenology 2002; 58:1349-60.
12. Huo LJ, Yang ZM. Effects of platelet activating factor on capacitation and
acrosome reaction in mouse spermatozoa. Mol Reprod Dev 2000; 56:436-40.
13. Huo LJ, Yang ZM. PGI(2)-PPAR delta signal transduction pathway in mammalian
embryo implantation. Progress in Biochemistry and Biophysics 2003; 30:522-6
14. Huo L-J, Yu L-Z, Liang C-G, Fan H-Y, Chen D-Y, Sun Q-Y. Cell-cycle-dependent
subcellular localization of cyclin B1, phosphorylated cyclin B1 and p34cdc2
during oocyte meiotic maturation and fertilization in mouse. Zygote 2005;
13:45-53.
15. Huo L-J, Yue K-Z, Yang Z-M. Characterization of viability, mitochondrial activity,
acrosomal integrity and capacitation status in boar sperm during in vitro storage
at different ambient temperatures. Reprod Fertil Dev 2002; 14:509-14.
16. Huo L-J, Zhong Z-S, Liang C-G, Wang Q, Yin S, Ai J-S, Yu L-Z, Chen D-Y, Schatten H,
Sun Q-Y. Degradation of securin in mouse and pig oocytes is dependent on
ubiquitin-proteasome pathway and is required for proteolysis of the cohesion
subunit, Rec8, at the metaphase-to-anaphase transition. Front Biosci 2006;
11:2193-202.
17. Liang C-G, Huo L-J, Zhong Z-S, Chen D-Y, Schatten H, Sun Q-Y. Cyclic adenosine
3',5'-monophosphate-dependent activation of mitogen-activated protein kinase
in cumulus cells is essential for germinal vesicle breakdown of porcine
cumulus-enclosed oocytes. Endocrinology 2005; 146:4437-44.
18. Quan H-M, Fan H-Y, Meng X-Q, Huo L-J, Chen D-Y, Schatten H, Yang P-M, Sun
Q-Y. Effects of PKC activation on the meiotic maturation, fertilization and early
embryonic development of mouse oocytes. Zygote 2003; 11:329-37.
19. Xiang Y, Takeo S, Florens L, Hughes SE, Huo L-J, Gilliland WD, Swanson SK, Teeter
K, Schwartz JW, Washburn MP, Jaspersen SL, Hawley RS. The inhibition of polo
kinase by matrimony maintains G2 arrest in the meiotic cell cycle. PLoS Biol 2007;
5:e323.
20. Yu L-Z, Xiong B, Gao W-X, Wang C-M, Zhong Z-S, Huo L-J, Wang Q, Hou Y, Liu K,
Liu XJ, Schatten H, Chen D-Y, Sun Q-Y. MEK1/2 regulates microtubule
organization, spindle pole tethering and asymmetric division during mouse
oocyte meiotic maturation. Cell Cycle 2007; 6:330-8.
21. Zhang M, Tao Y, Zhou B, Xie H, Wang F, Lei L, Huo L, Sun Q, Xia G. Atrial
natriuretic peptide inhibits the actions of FSH and forskolin in meiotic
maturation of pig oocytes via different signalling pathways. J Mol Endocrinol
2005; 34:459-72.
22. Zhong ZS, Huo LJ, Liang CG, Chen DY, Sun QY. Small GTPase RhoA is required for
ooplasmic segregation and spindle rotation, but not for spindle organization and
chromosome separation during mouse oocyte maturation, fertilization, and
early cleavage. Mol Reprod Dev 2005; 71:256-61.
Contact details
Li-Jun Huo. Ph.D, Professor
College of Animal Science and Technology
Huazhong Agricultural University
Wuhan 430070, Hubei, P.R.China
Tel: (86)-18986207556; (8627)-87288376 (O)
Email: huolijun@yahoo.com.cn