-1-
Table S8. HpaNoco2-inducible AtWRKY genes identified in this study
AtWRKY#*
25
(sub)group
I
described defense related roles
references
substrate of MAP kinase MPK4
suppression of SA-dependent defense responses
suppression of basal defense to Pst
(Andreasson et al.,
2005; Zheng et al.,
2007)
26
32
33
I
I
I
18
IIa
40
IIa
substrate of MAP kinase MPK4
suppression of SA-dependent defense responses
suppression of basal defense to Pst
activation of JA-dependent defense responses
activation of resistance to necrotrophic fungi
suppression of basal defense to Pst & Golovinomyces orontii
positive regulator of SAR
target of NPR1
suppression of basal defense to Pst & Golovinomyces orontii
60
IIa
suppression of basal defense to Pst & Golovinomyces orontii
6
IIb
Activation of defense-related PR1 expression
47
61
72**
8
28
48
IIb
IIb
IIb
IIc
IIc
IIc
71
75
IIc
IIc
11
IId
15
17
IId
IId
22
38
IIe
III
46
53
III
III
54
70
III
III
activation of basal defense to RKN and Hpa
suppression of basal resistance to Pst
suppression of SA-dependent defense responses
activation of SA-dependent defense responses
activation of basal defense to Pst
suppression of basal defense to Pst
suppression of SA-dependent defense responses
activation of JA-dependent defense responses
suppression of basal resistance to Pst
suppression of SA-dependent defense responses
activation of JA-dependent defense responses
immediate early flg22 inducible gene
target of NPR1
suppression of basal defense to Pst
suppression of SA-dependent defense responses
target of NPR1
activation of basal defense to Pst
target of NPR1
activation of basal defense to Pst, Erwinia carotovora, Erysiphe
cichoracearum & Botrytis cinerea
suppression of basal defense to Alternaria brassicicola
activation of basal and RPP4-mediated resistance to Hpa
activation of SA-dependent defense responses
suppression of JA-dependent defense responses
target of NPR1
(Andreasson et al.,
2005)
(Zheng et al., 2006)
(Wang et al., 2006;
Xu et al., 2006;
Shen et al., 2007)
(Xu et al., 2006;
Shen et al., 2007)
(Xu et al., 2006;
Shen et al., 2007)
(Robatzek and
Somssich, 2002)
this study
(Xing et al., 2008)
(Encinas-Villarejo
et al., 2009)
(Journot-Catalino
et al., 2006)
(Journot-Catalino
et al., 2006)
(Wang et al.,
2006);
(Kim et al., 2008)
(Wang et al., 2006)
(Wang et al., 2006)
(Li et al., 2004;
Abuqamar et al.,
2006; Li et al.,
2006a; Wang et al.,
2006; Knoth et al.,
2007)
transcript levels of all listed genes (except AtWRKY71) were significantly upregulated in ATH1 microarray experiments
**: not significantly up-regulated by HpaNoco2 in microarray experiments, but
strongly induced by this pathogen in RT-PCRs and qPCRs; see Figure 4.
*:
1
-2References
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Encinas-Villarejo, S., Maldonado, A.M., Amil-Ruiz, F., de los Santos, B.,
Romero, F., Pliego-Alfaro, F., Munoz-Blanco, J. and Caballero, J.L. (2009)
Evidence for a positive regulatory role of strawberry (Fragaria · ananassa)
FaWRKY1 and Arabidopsis AtWRKY75 proteins in resistance. J. Exp. Bot.
60, 3043–3065.
Kim, K.C., Lai, Z., Fan, B. and Chen, Z. (2008) Arabidopsis WRKY38 and
WRKY62 transcription factors interact with histone deacetylase 19 in basal
defense. Plant Cell, 20, 2357–2371.
Li, J., Brader, G. and Palva, E.T. (2004) The WRKY70 transcription factor: a
node of convergence for jasmonate-mediated and salicylate-mediated
signals in plant defense. Plant Cell, 16, 319–331.
Robatzek, S. and Somssich, I.E. (2002) Targets of AtWRKY6 regulation during
59 plant senescence and pathogen defense. Genes Dev. 16, 1139–1149.
Xing, D.H., Lai, Z.B., Zheng, Z.Y., Vinod, K.M., Fan, B.F. and Chen, Z.X.
(2008)Stress- and pathogen-induced Arabidopsis WRKY48 is a transcriptional
activator that represses plant basal defense. Mol. Plant, 1, 459–470.
Zheng, Z., Qamar, S.A., Chen, Z. and Mengiste, T. (2006) Arabidopsis
WRKY33 transcription factor is required for resistance to necrotrophic fungal
pathogens. Plant J. 48, 592–605.
Zheng, Z., Mosher, S.L., Fan, B., Klessig, D.F. and Chen, Z. (2007) Functional
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Pseudomonas syringae. BMC Plant Biol. 7, 2.
2