FACS SORTING REQUEST FORM
NAME:
_________________________
GROUP: ______________
DATE OF SORT:
______________________
Ext: ______
TIME OF SORT:
______________________
(Check Beatson Scheduler)
DETAILS OF CELL TYPE
HUMAN
OTHER
RAT
MOUSE
Sp Specify: __________
CELL LINE
Specify Origin of Tissue/Cell Line: ___________________________
(e.g. lymph node/ HeLa)
Have cells been modified in any way?
No
Yes
If Yes, nature of modification (i.e. transfection with adenovirus): _________________
________________________________________________________________________
Any possible hazards to user?
If Yes, describe:
No
Yes
__________________________________
Any precautions required by user?
No
Yes
If Yes, describe (e.g. face mask) : __________________________________
DETAIL OF SORT
How many tubes to be sorted? ________________ Fraction to be sorted_____________
Duration of sort?
_________________________
( Note: Duration of sort depends on number of tubes and the volumn in each tube; allow 30min
for 1ml and book the time accordingly. The sorter also requires at least 1 hour set up time. Book
the Aria for an hour from 7am to 8am and an assistant eg: Margaret O’Prey so the machine can
be QC tested and set up. You do not have to be present for the machine set up.)If the machine
has not been set up in the morning you will have to add an hour to your sort request time to allow
for this.
SORTING REQUIREMENTS
1.
10 x 106 cells/ml. Concentrate the sample.
2.
No more than 4mls of media/buffer in sterile FACS tubes. (available from stores).
3.
Cell must be filtered immediately before sort (use 40um cell strainer available from
stores)
Note: Cells are strained and then brought to FACS room, after 20min 2 nd tube is
strained and so on. This is to prevent cell clumping and hence blocking of the machine.
4.
Bring tubes for collection of cells; 2 tubes per fragment collected; 2mls of media/buffer
per tube.
5.
Add appropriate antibiotics to sample
6.
You MUST have control samples –ve and +ve
7. BE PUNCTUAL !!!!