Hellenic Accreditation System
Annex F1/6 to the Certificate No. 417-2
SCOPE of ACCREDITATION
of the
Laboratory for Animal Health
of the
Veterinary Services
of the
Ministry of Agriculture, Natural Resources and Environment of
Cyprus
Materials/Products tested
Types of test/Properties measured
Applied methods/Techniques used
Microbiological tests
1. Blood serum samples of
domestic or wild ruminants,
equine, swine, camelids,
carnivores and human origin
2. Isolates of Salmonella
spp, Campylobacter coli and
Campylobacter jejuni from
any primary material
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1. Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Rose Begal Test (ΜΕΘ ΕΒΟ-01)
according to Commission Decision
of 10 December 2008 amending
Annex C to Council Directive
64/432/EEC and Decision
2004/226/EC as regards diagnostics
test for bovine brucellosis
2008/984/EC.
2. Detection of antibodies against Βrucella
abortus/ melitensis/ suis
Complement Fixation Test (ΜΕΘ
ΕΒΟ-02) according to Commission
Decision of 10 December 2008
amending Annex C to Council
Directive 64/432/EEC and Decision
2004/226/EC as regards diagnostics
test for bovine brucellosis
2008/984/EC.
1. Detection of antibiotic resistance of
Salmonella spp with the Minimum
Inhibition Concentration method (MIC)
MIC method (ΜΕΘ ΕΒΟ-03)
according to the valid edition of
M100, M31 and M7 standards of the
Clinical and Laboratory Standards
Institute (CLSI), (annex 2.1,2.2,2.3)
manufactured by TREK Diagnostic
Systems, called Sensititre and the
Decision 2007/407/EC (annex 2.4).
Annex F1/6 to the Certificate ESYD No.417-2
26.09.2014
Materials/Products tested
Types of test/Properties measured
2. Detection of antibiotic resistance of
Campylobacter coli and Campylobacter
jejuni with the Minimum Inhibition
Concentration method (MIC)
Applied methods/Techniques used
MIC method (ΜΕΘ ΕΒΟ-04)
according to the valid edition of
M100, M31 and M7 standards of the
Clinical and Laboratory Standards
Institute (CLSI), (annex 2.1,2.2,2.3)
manufactured by TREK Diagnostic
Systems, called Sensititre and the
Decision 2007/516/EC (annex 2.4).
Virological tests
1. Blood serum samples or
plasma of bovine, ovine,
caprine, swine and equine
origin
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1. Detection of antibodies against Bovine
Leucosis Virus
ELISA Test (ΜΕΘ ΕΙ-01) according
to the Commission Directive
64/432/EC, the Decision
2009/976/ΕΕ and the OIE Manual of
Diagnostic Tests and Vaccines for
Terrestrial Animals 2012.
2. Detection of antibodies against
Bluetongue Virus
Competitive ELISA Test (ΜΕΘ ΕΙ02) according to the Commission
Directive 92/119/EC and the OIE
Manual of Diagnostic Tests and
Vaccines for Terrestrial Animals
2012.
3. Detection of antibodies against African
Swine Fever Virus
Competitive ELISA Test (ΜΕΘ ΕΙ03) according to the Commission
Directive 2002/60/EC and the OIE
Manual of Diagnostic Tests and
Vaccines for Terrestrial Animals
2012.
4. Detection of antibodies against Swine
Vesicular Virus
Competitive ELISA Test (ΜΕΘ ΕΙ05) according to the Commission
Decision 2000/428/ΕC and the OIE
Manual of Diagnostic Tests and
Vaccines for Terrestrial Animals
2012.
5. Detection of antibodies against Caprine
Arthritis Encephalitis and Maedi- Visna
Virus
Indirect ELISA Test (ΜΕΘ ΕΙ-16)
according to the Commission
Directive 91/68/ΕEC and the OIE
Manual of Diagnostic Tests and
Vaccines for Terrestrial Animals
2012.
6. Detection of antibodies against Classical
Swine Fever Virus
Competitive ELISA Test (ΜΕΘ ΕΙ04) according to the Commission
Directive 2001/89/EC, the
Commission Decision 2002/106/ΕC
- annex 2.1 and the OIE Manual of
Diagnostic Tests and Vaccines for
Terrestrial Animals 2012.
Annex F1/6 to the Certificate ESYD No.417-2
26.09.2014
Materials/Products tested
Types of test/Properties measured
7. Detection of antibodies against African
Horse Sickness Virus
Applied methods/Techniques used
Competitive ELISA Test (ΜΕΘ ΕΙ11) according to the Commission
Decision 2002/106/ΕC - annex 2.1
and the OIE Manual of Diagnostic
Tests and Vaccines for Terrestrial
Animals 2012.
Biological tests
1.Nervous tissue (obex) of
bovine, ovine and caprine
origin
1. Detection of the protein PrPRes
Immuno-enzymatic technique ΕΙΑ
(TeSeE BIO- RAD) (ΜΕΘ ΕΓΚ-01)
according to the Commission
Regulation 999/2001/EC, Annex X,
chapter C.
2.Feeding stuffs
1.Detection of animal proteins
Microscopic detection (ΜΕΘ
ΕΠΑΒΠ-01) according to the
Commission Regulation
51/2013/EC, chapter VI.
Histological tests
1.Nervous tissue of ovine,
caprine and bovine origin
1. Detection of lesions caused by PrPsc
agent in ruminants
Histological examination (ΜΕΘ
ΕΙΣT- 01) with haematoxylin- eosin
staining according to the «Fixation,
tissue process histology and
immunohistochemistry procedures
for the diagnosis of BSE and Scrapie
– Veterinary Laboratory
Agency CRL, EU community
reference laboratory for TSE, July
2005» and the Commission
Regulation 999/2001/EC, annex X,
chapter C.
2. Nervous and lymphoid
tissue of caprine and bovine
origin
1. Detection/ confirm of PrPsc agent in
small and large ruminants.
Immunohistochemistry examination
(ΜΕΘ ΕΙΣT- 02)«Fixation, tissue
process histology and
immunohistochemistry procedures
for the diagnosis of BSE and Scrapie
– Veterinary Laboratory Agency
CRL, EU community reference
laboratory for TSE, July 2005»,
VIIth Intrnational workshop on the
Diagnostics of Spongiform
Encephalopathies, V.I.A November
2003, OIE Manual of Diagnostic
Test and Vaccines for Terrestrial
Animals, 7th edition 2012,
Regulation (EC) no 999/2001,
Workshop on
Immunohistochemistry for TSE
diagnosis VLA, October 2005.
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Annex F1/6 to the Certificate ESYD No.417-2
26.09.2014
Site of assessment: Permanent laboratory premises, Veterinary Services, Athalassa, 1417, Cyprus.
Approved signatories :
1. V. Christodoulou, Bacteriology, Serology Laboratory (EBO)
2. P. Stylianou and S. Stavri, Transmissible Spongiform Encephalopathies Laboratory (ΕΕΓΚ)
3. A. Mazeris, V. Christodoulou, Virology Laboratory (EI)
4. S. Georgiadou, Histopathology Laboratory (ΕΙΣΤ)
5. I. Ioannou and P. Konstantinou, Pathology, Bacteriology and Parasitology Laboratory (ΕΠΑΒΠ)
This Scope of Accreditation replaces the previous one dated 04.07.2013.
The Accreditation Certificate No. 417-2, to ELOT EN ISO/IEC 17025:2005, is valid until 31.12.2014.
Athens, September 26, 2014
Ioannis Sitaras
Director of the Laboratories Accreditation Division
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Annex F1/6 to the Certificate ESYD No.417-2
26.09.2014