Mouse Model - Transgenic Request
Animal Care Services
Mouse Models Core
PI: ___________________________________
Date Submitted: ________________
Construct name (short w/ no unusual characters): _______________________________
Contact person: _________________________________________________
Contact person info:
Phone #1: ___________________
Phone #2: __________________
FAX: ______________________
Pager: _____________________
Email:___________________________________________________
SERVICE DESCRIPTION:
- Plasmid or BAC transgenes will be injected for gene transfer into the pronuclei of
fertilized, viable murine oocytes.
- Standard mouse strains available for injection are FVB/NJ (inbred) or B6D2F1/J.
- 30 live weaned pups will be produced from the injected oocytes, to screen for
expression of the transgene
- The fee includes the service of DNA purification by our designated lab. Be advised, if
you choose to purify your DNA yourself, our guarantee of 30 live births will be nullified.
- Tail snips from each resulting mouse will be taken upon weaning and given to
investigators to detect founder animals.
- The use of nonstandard strains for oocyte injection will be reviewed on an individual
basis
- Before service, you will need to provide some information about the transgene and
some administrative details. Please fill out this form and then contact the Mouse Models
Core Facility. The manager will arrange a meeting with you to discuss scheduling your
injections, transfer the DNA, and to review the construct information.
- All services are performed in the order received and are kept confidential.
Questions and Contact info for this service: ACS-MMC@acs.ufl.edu
The fee for this service, as well as the electronic version of this form, can be found at
the following website: http://acs.ufl.edu/MouseModelCore/index.shtml
Which strain of mice for embryo donors for injections?
FVB/N (inbred) _______________
B6D2F2J (hybrid) _____________
Other (please specify) ___________
FMACS243-01
1 of 3
Mouse Model - Transgenic Request
Animal Care Services
Mouse Models Core
GENE STRUCTURE: Diagram the transgene construct and label salient features,
including the restriction sites used to release the transgene construct, the promoter,
reporter, stop and start sites for transcription, stop and start sites for translation,
intron/exon boundaries, and size of each sequence.
SPECIAL CONSIDERATIONS/INSTRUCTIONS: Please indicate below any special
instructions that we may need to carry out during any portion of the assigned project:
FMACS243-01
2 of 3
Mouse Model - Transgenic Request
Animal Care Services
Mouse Models Core
BILLING INFORMATION
PI: ________________________________
The following Chart Field Stream information must be provided for billing:
* Department ID:
* Fund Code:
* Program Code:
Source of Fund:
Project Code:
Flex Code:
(* items are required)
If the work will be paid for with funds from outside of the University of Florida, they
must be paid by check in advance to the ACS Business Office; (352) 273-9252.
PI signature: ___________________________________________
ANIMAL TRANSFER: We will automatically assign you a unique breeding project #
and transfer any transgenic founder animals produced to our breeding protocol for test
breeding upon weaning unless you instruct us otherwise below. We can test breed
founders and subsequent germline transgenics for you at minimal extra cost to you.
Before we begin, we will need an Institutional Animal Care and Use (IACUC) protocol
number for your project, and a location to transfer the mice. The IACUC protocol need
only be for the analysis of the animals - the Core Facility has IACUC approved protocols
for the procedures used to generate and breed the animals for you.
IACUC number_____________________________
Have ACS test breed founders upon weaning? __________________
If “yes,” which background strain to test breed to? _______________________________
If “No” to above, transfer animals to Building: ________________ Room #: __________
FMACS243-01
3 of 3