Supplemental Table 1: Clinical status of mice 45 hours after induction of pneumococcal meningitis
Experimental
Total clinical
Group [n]
score
PBS-injected
Beam walk test b
Postural reflex
Reduced
Presence of
Presence of
text c
vigilance
pilo-erection
seizures
[0, 12] a
[0, 6]
[0, 3]
[0, 1]
[0, 1]
[0, 1]
0.0
0.0
0.0
0.0
0.0
0 of 4
5.5  1.7 c
2.7  1.4
0.9  0.7
0.9  0.3
1.0  0.0
0 of 10
3.4  0.9 *
1.4  0.9
0.1  0.4 * #
0.9  0.4
0.9  0.4
0 of 8
4.1  1.6
1.9  0.9
1.0  0.5
0.7  0.5
0.6  0.5
0 of 9
1.9  0.9 # *
0.7  0.5 # *
0.1  0.3 # *
0.6  0.5
0.7  0.5
0 of 9
controls [4]
Infected mice treated
with the vehboxA [10]
Infected mice treated
with box A [8]
Infected mice treated
with RLS [9]
Infected mice treated
with REPS [9]
NOTES:
a
In healthy animals, the score is 0 points. The maximum total score value is 12. Mice with 11 or more score points were considered
terminally ill and euthanized due to ethics. None of the investigated mice showed scores above 10 within the observation period. The values in
brackets show minimum and maximum values.
b
The goal of the beam walk test for a mouse is walking on wooden beams with decreasing
diameters (13 mm, 9 mm, or 5 mm in diameter). The score is 0 or 1, if a mouse was able to traverse or stay on a beam of 5 mm in diameter. A
score value of 2 or 3 is attributed to a mouse able to walk or stay on the 9 mm-beam. Failure of walking or staying along the thickest beam, the
score assigned is 5 or 6, respectively.
c
For postural reflex test, mice are lifted upon fixation of the tail and symmetry in the movement of the
four limbs is examined. Score 0 indicates that all four limbs extended symmetrically, 1 = limbs on one side extended to a lesser degree or
more slowly than those on the other side, 2 = minimal movement of limbs of one or both sides, and 3 = lack of movement of limbs of one or
both sides. The other measures are observational in nature, indicating the presence or absence of the respective clinical symptom. Above all,
the scoring system was designed to capture neurological (motor) disabilities, explaining the high number of points obtainable in the two motor
tests (beam walk and postural reflex test). The other parameters like the presence of seizure are added in order to provide a broader picture of
the clinical status of the mice. * p < 0.05, compared to infected mice treated with the vehboxA, and # p < 0.05, compared to infected mice treated
with RLS, using Kruskal Wallis ANOVA and Mann Whitney U test plus alpha corrections for post hoc analysis.
deviations.
c
Data are means  standard