maximeTM PCR PreMix (i-StarMAX II)
Maxime PCR PreMix Kit has not only various kinds of PreMix Kit according to experimental purposes,
but also a 2X Master mix solution.
Maxime PCR PreMix Kit(i-StarMAXTM II) is made from iNtRON's i-StarMAXTM II DNA Polymerase. iStarMAXTM II DNA Polymerase is an optimal enzyme mixture of i-StarTaqTM DNA Polymerase and
proofreading DNA polymerase. i-StarTaqTM DNA Polymerase is a thermo-stable, modified from of
recombinant Taq DNA Polymerase suitable for hot start PCR. As a result of addition of i-StarTaqTM
DNA Polymerase, i-StarMAXTM II DNA Polymerase cannot be useful tool only in amplification of short
and long fragments but also problematic template/primer systems. High yields of PCR product can
be achieved using extension times as short as from 30 seconds to 1 minute per kb per cycle with
the i-StarMAXTM II DNA Polymerase. The i-StarMAXTM II DNA Polymerase is recommended for
relatively rapid, high-fidelity amplification of PCR targets up to 15kb when proofreading DNA
polymerase alone requires too long an extension time or yields are insufficient. Maxime PCR
PreMix(i-StarMAXTM II) is the product what is mixed every component: i-StarMAXTM II DNA
Polymerase, dNTP mixture, reaction buffer, and so on- in one tube for 1 rxn PCR. This is the
product that can get the best result with the most conveninece system. The first reason is that it has
every components for PCR, so we can do PCR just add a template DNA, primer set, and D.W.. The
second reason is that it has Gel loading bufffer to do electrophoresis, so we can do gel loading
without any treatment. In addition, each batches are checked by a thorough Q.C., so its
reappearance is high. It is suitable for various sample's experience by fast and simple using method.

High fidelity, specificity, and yield

Versatile for various DNA template including cloned fragment, phage DNA, mammalian
genomic DNA and etc.

Ideal for difficult templates, such as GC-rich or looped sequences

Ready to use : only template and primers are needed

Stable for over 1 year at -20℃

Time-saving and cost-effective
* MaximeTM PCR PreMix (i-StarMAXTM II ; for 20 μl rxn)
* i-StarMAXTM (II) DNA Polymerase
* dNTPs
96 tubes
2.5 U
2.5 mM each
* Reaction Buffer
1x
* Gel Loading buffer
1x
Amplification of various Hot-Start PCR condition & LA PCR (20kb)
[Panel A]
[Panel B]
Fig 1. Amplification of various Hot-Start PCR condition & LA PCR (20Kb)
[Panel A] Comparison of PCR amplification with supplier A
Lane M, 100 bp Ladder DNA Marker;
Lane 1, 161 bp; Lane 2, 165 bp; Lane 3, 166 bp; Lane 4, 181 bp; Lane 5, 218 bp; Lane 6, 266 bp
[Panel B] Amplification of 20Kb PCR fragment
Lane M, λ/Hind III Marker;
Lane 1, 1 ng of λDNA; Lane 2, 100 pg of λDNA; Lane 3, 10 pg of λDNA; Lane 4, 1 pg of λDNA;
Lane 5, 100 fg of λDNA