1 Titles and Legend to Supplementary Figures 2 3 Supp. Figure 1. Correlogram of microsatellite genotype spatial autocorrelation 4 across 20 distance classes (in metres), with error bars developed from 5 bootstrapping and a 95% confidence interval about the null hypothesis. Error 6 bars that do not overlap 0 and/or calculated r outside of the confidence 7 bounds indicate significant spatial-genetic structure. 8 9 Supp. Figure 2. Kinship estimator Ritland (1996) (FR) plotted against the 10 logarithm of geographic distance (in metres). A test of the slope of the linear 11 regression line (rR) indicates a significant relationship between kinship and 12 geographic distance. 13 14 Supp. Figure 3. Relationship estimator Moran’s-I (RM) plotted against the 15 logarithm of geographic distance (in metres). A test of the slope of the 16 regression line (rM) indicates a significant relationship between Moran’s-I and 17 geographic distance. 18 19 Supp. Figure 4. A graph of increasing cumulative distance classes from 400 20 to 5200 m and the spatial autocorrelation (r) of binary mtDNA haplotype 21 differences with distance. Error bars developed from bootstrapping and upper 22 and lower limits of a 95% confidence interval about the null hypothesis are 23 included. 24 1 25 Supp. Figure 5. Correlogram of spatial autocorrelation (r) of binary mtDNA 26 haplotype distance across 20 distance classes (in metres), with error bars 27 developed from bootstrapping and upper and lower limits of a 95% 28 confidence interval about the null hypothesis. 29 2 30 Supplementary Table 1. Spatial autocorrelation analysis of microsatellite 31 genotypes using increasing distance class sizes, with values of the spatial 32 autocorrelation statistic (r), the upper and lower bounds of the null 33 distribution, the probability (P) that r is greater than permuted r, and sample 34 sizes. Distance (m) 0 to 100 0 to 200 0 to 400 0 to 600 0 to 800 0 to 1000 0 to 1200 0 to 1400 0 to 2000 0 to 3000 0 to 4000 0 to 5000 0 to 5400 r 0.007 0.010 0.003 0.004 0.004 0.003 0.003 0.002 0.002 0.002 0.001 0.000 0.000 Upper 0.010 0.006 0.003 0.003 0.002 0.002 0.001 0.001 0.001 0.001 0.001 0.000 0.000 Lower -0.010 -0.006 -0.003 -0.002 -0.002 -0.001 -0.001 -0.001 -0.001 -0.001 -0.001 0.000 0.000 P 0.092 0.003 0.030 0.002 0.001 0.001 0.002 0.005 0.003 0.004 0.014 0.614 0.926 N 584 1441 3921 6059 7785 9231 10179 10760 11465 11988 14380 16826 17020 35 3 36 Supplementary Table 2. Spatial autocorrelation statistics (r) for microsatellite 37 genotypes by distance class, upper (U) and lower (L) bounds of the null 38 distribution, significance of a one-tailed test of positive autocorrelation (P), 39 and sample sizes of pair-wise contrasts per distance class (N). 40 Distance (m) 140 223 295 358 435 516 595 694 793 901 1033 1228 1587 2995 3462 3769 4050 4285 4599 r 0.012 0.005 -0.004 -0.004 0.013 0.001 0.007 0.003 0.006 -0.004 -0.002 -0.002 -0.006 -0.005 0.000 -0.003 -0.005 -0.010 -0.004 U 0.009 0.009 0.008 0.009 0.009 0.008 0.008 0.008 0.009 0.009 0.009 0.008 0.008 0.007 0.008 0.008 0.009 0.008 0.008 L -0.008 -0.008 -0.009 -0.008 -0.009 -0.008 -0.009 -0.008 -0.009 -0.008 -0.009 -0.009 -0.008 -0.008 -0.008 -0.008 -0.008 -0.008 -0.008 P 0.004 0.137 0.830 0.800 0.004 0.403 0.059 0.227 0.088 0.847 0.670 0.642 0.921 0.884 0.510 0.767 0.869 0.985 0.816 N 892 856 850 860 850 850 859 864 851 853 849 848 848 849 854 848 849 848 848 41 4 42 Supplementary Table 3. Spatial autocorrelation statistic (r) of mtDNA binary 43 haplotypes across increasing distance classes, with the upper (U) and lower 44 (L) bounds of the null distribution, the probability (P) that r is greater than 45 permuted r, and pairwise sample sizes (N). Distance (m) 0 to 400 0 to 800 0 to 1200 0 to 1600 0 to 2000 0 to 2400 0 to 2800 0 to 3200 0 to 3600 0 to 4000 0 to 4400 0 to 4800 0 to 5200 r 0.155 0.100 0.116 0.108 0.096 0.091 0.091 0.085 0.064 0.051 0.032 0.010 0.002 U 0.089 0.057 0.043 0.039 0.037 0.034 0.041 0.031 0.029 0.021 0.018 0.011 0.003 L -0.067 -0.036 -0.023 -0.018 -0.015 -0.014 -0.017 -0.016 -0.017 -0.014 -0.010 -0.009 -0.004 P 0.003 0.002 0.001 0.001 0.001 0.004 0.003 0.002 0.002 0.003 0.003 0.028 0.171 N 216 451 623 687 708 721 734 769 840 941 1052 1142 1213 46 5 47 Supplementary Table 4. Spatial autocorrelation statistics (r) for binary mtDNA 48 haplotype distances, upper and lower bounds of the null distribution, p-values 49 for a one-tailed test of positive genetic autocorrelation versus null distribution, 50 and sample sizes per distance class (N) for the correlogram. Distance (m) 255 421 585 759 954 1215 2622 3517 3938 4275 4719 r 0.209 0.146 -0.136 0.175 0.109 0.159 0.008 -0.020 -0.089 -0.165 -0.160 U 0.127 0.131 0.119 0.121 0.132 0.125 0.111 0.118 0.134 0.130 0.121 L -0.100 -0.118 -0.121 -0.126 -0.132 -0.130 -0.134 -0.134 -0.134 -0.138 -0.133 P 0.004 0.015 0.986 0.004 0.054 0.010 0.456 0.679 0.920 0.989 0.988 N 130 99 99 100 99 99 100 99 99 99 101 51 6