WINONA STATE UNIVERSITY PROPOSAL FOR NEW COURSES Department _Biology_________________________________________ Date __28 October 2004_______ ___311________________ Course No. ___Genetics Laboratory_______________________________ _____1SH_____ Course Name Credits This proposal is for a(n) ___X__ Undergraduate Course Applies to: __X__ Major _____ Required __X Elective ______ Graduate Course __X__ Minor _____ Required __X__ Elective ______ University Studies* Prerequisites __BIOL 241 Basics of Life, BIOL 242 Organismal Diversity, BIOL 310_Genetics (or concurrent registration in BIOL 310) Grading method __X___ Grade only ______ P/NC only ______ Grade and P/NC Option Frequency of offering _Offered yearly____________________________ *For University Studies Program course approval, the form Proposal for University Studies Courses must also be completed. submitted according to the instructions on that form. Provide the following information (attach materials to this proposal): A. Course Description 1. 2. 3. 4. 5. 6. B. Rationale 1. 2. 3. C. Statement of the major focus and objectives of the course. Specify how this new course contributes to the departmental curriculum. Indicate any course(s) which may be dropped if this course is approved. Impact of this Course on other Departments, Programs, Majors, or Minors 1. 2. D. Catalog description. Course outline of the major topics and subtopics (minimum of two-level outline). Basic instructional plan and methods. Course requirements (papers, lab work, projects, etc.) and means of evaluation. Course materials (textbook(s), articles, etc.). List of references. Does this course increase or decrease the total credits required by a major or minor of any other department? If so, which department(s)? List the departments, if any, which have been consulted about this proposal. University Studies Course Proposals The form Proposal for University Studies Course must also be completed and submitted according to the instructions on that form. Attach a Financial and Staffing Data Sheet. Attach an Approval Form. Department Contact Person for this Proposal: _Lawrence A. Reuter___________________ Name (please print) __457-5279______ Phone _____LReuter@winona.edu e-mail address A. Course Description 1. Catalog description. 311 Genetics Laboratory - 1 SH. An optional laboratory to accompany Genetics 310. Includes experiments from classical, molecular and cytogenetics. Prerequisite: Concurrent or previous enrollment in Genetics 310 and permission of instructor. 2. Course outline of the major topics and subtopics (minimum of two-level outline). Week of (2004): Lab Topic (A) D. Melanogaster Crosses: Jan 12 Introduction, Lab notebooks Fly Handling - distinguishing males/female; collect virgins on your own Examine wmf phenotypes; Flies for Salivary Glands - serial transfer Jan 19 Clear out parents from previous crosses (B) D. Melanogaster Polytene Chromosome Observations: Prepare Salivary Gland Squashes; Identify Chromosomes (C) D. Melangaster Genetic Analysis: Jan 26 Observe F1's; Set up F2's (problem set - review F2 ratios, Linkage, Epistasis - in lab notebook) Feb 2 Aldehyde Oxidase (AO) populations - Electrophoresis Feb 9 Observe F2's and make counts Feb 16 Analyze all data for all crosses Feb 23 Separation of Drosophila Pigments - Chromotography Karyotyping exercise (on your own) Mar 1 DNA Isolation Mar 15 RAPD-PCR of AO flies (set-up takes ~1/2 hr + 15 min the next AM) Proposal Planning & Set-up for Individual projects Mar 22 Electrophoresis and Staining of PCR products Continue Proposal Planning & Set-up (from AO+/AO- flies) (D) Individual Projects: Mar 29 Project Proposal Due/Lab Notebooks Due Begin individual projects 3. Apr 5 Individual projects Apr 12 Individual projects Apr 19 Individual projects Apr 26 Final Project Presentation/Project Report Due Basic instructional plan and methods. This is an experimental laboratory course. Introductory lectures will be given on an as-needed basis. Students will initially follow instructor-designed experiments, to be followed by student-designed experiments. Careful manipulations, observations, recording of data and analyses of results are required. 4. Course requirements (papers, lab work, projects, etc.) and means of evaluation. Materials needed: Lab notebook (spiral or bound) – no looseleaf notebooks; floppy disk for storing digital images, calculator. Policies: Laboratory attendance is required. If you miss one lab you will have to make up the work. If you miss two labs your grade will be lowered. Grading: Lab Notebook Project proposal Project presentation Final project report 90% is sufficient for an A 5. 80% for a B 100 pts 20 pts 30 pts 30 pts 170 pts 70% for a C 60% for a D Course materials (textbook(s), articles, etc.). See WEB page: http://bio.winona.msus.edu/evenson/index.htm. Lab protocols, visual resources and handouts will be provided as needed. Much of the required laboratory information is available on the WWW (see references below). 6. List of references. http://flybase.bio.indiana.edu/ http://www.ornl.gov/sci/techresources/Human_Genome/genetics.shtml http://www.accessexcellence.com/RC/genetics.html http://www.dnaftb.org/dnaftb/ http://www.genome.gov/glossary.cfm http://www.nsgc.org/careers/index.asp B. Rationale 1. Statement of the major focus and objectives of the course. BIOL 311 Genetics Laboratory focuses on techniques of experimentation and analysis used in genetics research. The major objectives of this course include 1) that students demonstrate the complete practice of experimental science: designing experiments, performing experiments, making careful observations, accurately recording experimental data, analyzing experiments, and reporting the results of experiments in both the written and oral formats used in science. 2. Specify how this new course contributes to the departmental curriculum. Genetics is a central and fundamental aspect of biology and is required of all biology majors in our core curriculum. BIOL 311 Genetics Laboratory provides a setting for learning and practicing the techniques of genetic analysis. These techniques are frequently used in research laboratories for which our students seek employment after graduation with a degree in BIAH or BICM. 3. Indicate any course(s) which may be dropped if this course is approved. No course is expected to be dropped. If necessary, one laboratory section of BIOL 118 General Biology will be dropped to allow for the staffing for this course. C. Impact of this Course on other Departments, Programs, Majors, or Minors 1. 2. Does this course increase or decrease the total credits required by a major or minor of any other department? If so, which department(s)? List the departments, if any, which have been consulted about this proposal. None No. WINONA STATE UNIVERSITY APPROVAL FORM Routing form for new and revised courses and programs. Department Recommendation: Approved by the Biology Department via oral vote on 29 October 2004. _________________________________ Department Chair ________________ Date Dean’s Recommendation _____ Approved _________________________________ Dean of College A2C2 Recommendation For: Course or Program___BIOL 311 Genetics Laboratory 1SH__ _____ Disapproved ________________ Date _____ Approved _____ Major _____ Disapproved _____ Minor _________________________________ Chair of A2C2 ________________ Date Graduate Council Recommendation (if applicable) _____ Approved _________________________________ Chair of Graduate Council ________________ Date _________________________________ Director of Graduate Studies ________________ Date Faculty Senate Recommendation _____ Approved _________________________________ President of Faculty Senate _____ Disapproved _____ Disapproved ________________ Date Academic Vice President Recommendation _____ Approved ________________________________ Academic Vice President Decision of President _____ Approved _________________________________ President __LReuter@winona.edu_______________ e-mail address _____ Disapproved ________________ Date _____ Disapproved ________________ Date Please forward to Registrar. Registrar _________________ Date entered Please notify department chair via e-mail that curricular change has been recorded. WINONA STATE UNIVERSITY FINANCIAL AND STAFFING DATA SHEET Course or Program____BIOL 311 Genetics Laboratory 1SH__ Include a Financial and Staffing Data Sheet with any proposal for a new course, new program, or revised program. Please answer the following questions completely. Provide supporting data. 1. Would this course or program be taught with existing staff or with new or additional staff? If this course would be taught by adjunct faculty, include a rationale. This course will be taught with existing staff. 2. What impact would approval of this course/program have on current course offerings? Please discuss number of sections of current offerings, dropping of courses, etc. This 1SH course will have minimal new additional impact on our current course offerings: This course has been taught in the past as BIOL 311 Genetics Laboratory 1QH. With semester conversion we attempted to incorporate the molecular part of genetics into BIOL 307 Cell Biology Laboratory 1SH (five sections per year) and the population part of genetics in BIOL 313 Ecology Laboratory 1SH (three sections per year). However, neither of these two courses could find adequate time to add genetic experiments, which take weeks for even the short life-cycled flies. Also, students who take one of theses course do not necessarily take the other. Thus, experimental genetics was incompletely taught This course has been taught during the past year as a one-time offering and was desired and well received by students. 3. What effect would approval of this course/program have on the department supplies? Include data to support expenditures for staffing, equipment, supplies, instructional resources, etc. This course is expected to have minimal impact on departmental supplies. All three of our second-year laboratory courses are optional for most biology majors. If students elect to take one course in place of another, lab supplies are freed from one course and allocated to another course. Fly crosses and fly genetics are among the cheapest of our laboratory preparations. Laboratories will be managed for BIOL 311 as last year and previous years: The instructors of this course are responsible for giving detailed order information to the Laboratory Services Specialist for course materials that need to be ordered. Likewise, detailed instructions will be given to the Laboratory Services Specialist for routine course materials, such as amount and preparation of solutions and needed, shared biology equipment. The instructor will be responsible for care and use of any live course materials, including sub-cultures, collecting virgins, making crosses, etc.