WHONET 5.4 Update notes, June 2006 General Microbiology expert system: WHONET now has an expert system with new features in data entry, data analysis, and laboratory configuration. The three components of the expert system are: 1. microbiological isolate alerts of unusual or important findings; 2. expert interpretation rules (for MRSA, ESBL-producers, etc.); and 3. selective antibiotic reporting in clinical reports. These features are described at greater length below. New codes: Several new organisms, antibiotics, and specimen types have been added. Previously, the serotypes file included serotypes for Salmonella and S. pneumoniae. Serotypes for E. coli and Shigella have just been added. Multi-user WHONET: In earlier versions of WHONET, only one instance of WHONET could be run at a time. If users tried to start WHONET a second time, they would receive the message “WHONET is already running”. To avoid conflicts between users, it was generally recommended to install the WHONET program and configuration files separately onto the c: drive of each computer. A problem with this approach was the need for separate maintenance of the software and laboratory configuration files for each computer where WHONET was installed. In WHONET 5.4, this limitation has been removed, and should be particularly useful for institutions with a laboratory computer network. It is now possible to keep the WHONET program and configuration files on a common network drive without conflict between users. Advantages of this modification include: 1. For most software updates, it will only be necessary to update the central computer, not each computer separately; 2. All users will be sharing a set of laboratory configuration files, so updates to antibiotic lists, breakpoints, and other laboratory information will be automatically available for all software users. Note: It is still necessary to run the initial WHONET installation program separately for each computer because WHONET relies on a number of additional Microsoft and nonMicrosoft file “libraries” that must be installed in each computer’s Windows System folder. During the installation process, the installation software (InstallShield) will ask where you would like to install the software. The default is c:\whonet5, but you can change this to the common network location for shared use. For later re-installations or software updates, in most cases you will only need to update the one copy of the software on the central computer. An additional advantage of this enhancement for future software development is that a multi-user WHONET will facilitate internet-based access to WHONET analyses. Configuration Antibiotic panels: Panels have been added for “Gram positive urine”, “Shigella”, and “N. meningitidis”. “Modify laboratory”, “Antibiotics”, “Panels”. Antibiotic panels: In previous versions of WHONET, all organisms used the same sequence of antibiotics. Now, the user can specify a different sequence for each drug panel. For example in Staphylococci, ciprofloxacin may precede gentamicin on the data entry screen, but follow it for E. coli. “Modify laboratory”, “Antibiotics”, “Panels”, “Antibiotic sequence”. Conditional antibiotic reporting: In previous versions of WHONET, clinical reports included all antibiotics tested. In WHONET 5.4, the user may indicate which antibiotics should be included in the clinical report to be given to the physician. The user may specify “first-line antibiotics”, “second-line antibiotics”, and “do not print” antibiotics. The user can indicate the criteria to be used for printing “second-line antibiotics”. “Modify laboratory”, “Antibiotics”, “Panels”, “Conditional antibiotic reporting”. Cefuroxime sodium and cefuroxime axetil: The typical cefuroxime most frequently tested in clinical laboratories is cefuroxime sodium (code CXM). Cefuroxime axetil is an oral version of this antibiotic (code CXA), and is tested by some laboratories, usually in addition to cefuroxime sodium. Unfortunately, many laboratories incorrectly have chosen “cefuroxime axetil” in their antibiotic configuration when in reality they test “cefuroxime sodium” in the laboratory. To avoid confusion in the future, WHONET 5.4 will refer to “cefuroxime sodium” as “cefuroxime”. The antibiotic codes remain unchanged. Expert interpretation rules: WHONET 5.4 has a number of “expert rules” for modifying the interpretation of susceptibility test results. For example in the case of oxacillin- or cefoxitin-resistant staphylococci, WHONET can change the interpretation for all betalactam antibiotics to resistant. This feature is always activated for data entry and clinical reporting. For data analysis, the user can turn the feature on or off. There are also rules for “probable” and “confirmed” ESBL producers, beta-lactamase-negative ampicillin resistant H. influenzae, and MLS “DTest”-positive staphylococci and streptococci. “Modify laboratory”, “Antibiotics”, “Breakpoints”, “Expert interpretation rules”. Users may indicate rules that they do not wish to use. Isolate alerts: WHONET 5.4 now has approximately 190 pre-defined “expert rules” for alerting microbiologists and infection control staff of unlikely, unusual, and important findings. Alerts include: 1. Quality control alerts (“susceptible strains are rare”, “resistant strains are rare”, “the resistance pattern is rare”, “disk diffusion is not recommended for this organism and antibiotic”, “other problerm”); 2. Microbiological alerts (“important species”, “important resistance”, “save the isolate”, “send to a reference laboratory”, “other alert”); and 3. Clinical alerts (alert the infection control team, therapy comment). Alerts can be “high”, “medium”, or “low” priority. Users can deactivate rules that they do not with to use. The user may also define their own “user-defined alerts”. WHONET can use these alerts both in data entry and in data analysis. National networks can also develop their own set of expert rules to use by laboratories in the surveillance network. “Modify laboratory”, “Alerts”. Data entry and file management Antibiotic panels: Previously, WHONET displayed all antibiotics in the user’s laboratory configuration and “jump” to appropriate antibiotics according to the panel configuration for the organism selected. For example, if the user entered “sau” for S. aureus, WHONET would display all of the antibiotics, but jump only to the Gram-positive antibiotics configured by the user for “Staphylococcus”. In WHONET 5.4, the software will only display the drugs configured for the panel. So for “sau”, the user will only see the antibiotics configured for this organism. If the user does want to see and enter results for other antibiotics absent from the panel, the user can select “All antibiotics” from the option “Antibiotic panel”. Conditional antibiotic reporting: WHONET will display with a white space all antibiotics that will be included in the clinical report. Antibiotics appearing in gray do not appear in the clinical report for the clinician. The user can use Laboratory configuration to define the conditional antibiotic reporting rules. Or the user can use the <F8> and <F9> keys during data entry to manually select the antibiotics to be included in the clinical report. Isolate alerts: For isolates that meet the criteria of any of the active microbiological alerts, WHONET will print a message in the lower right-hand corner of the screen about the finding. For “high priority” alerts, the user will also receive a message box with the alert (for example “Vancomyin-resistant Staphylococcus”). When the user clicks on “Save isolate”, the user will see a summary of the alerts for the isolate. Expert interpretation rules: WHONET will apply the active expert interpretation rules when determining the test interpretation. For example, for an MRSA isolate, WHONET will automatically change the interpretations for all beta-lactam agents to resistant. View database: For convenience, WHONET now displays the patient and specimen identification numbers in the two left-most columns. Print clinical reports: In previous versions of WHONET, the user could print the “current isolate” or use criteria such as “specimen date”, “date of data entry”, “specimen number”, etc. to indicate which clinical reports to print. In WHONET 5.4, the user can also go to “View database”, and manually select a group of isolates to print. This can be done by using the “Shift” and “Ctrl” keys with the mouse to select the isolates to print. Clinical reports: In addition to the expert interpretation rules described above, WHONET has two additional rules for clinical reports to aid the use of information by clinicians. 1. S. pneumoniae: if the laboratory tests this organism with an oxacillin disk but does not determine a penicillin MIC, the clinical report will include both oxacillin (susceptible or non-susceptible) and penicillin (with the same interpretation given for oxacillin); and 2. Staphylococcus (sau, sep, scn): if the laboratory tests cefoxitin but not oxacillin, WHONET will include both cefoxitin and oxacillin in the clinical report, using the cefoxitin interpretation. Encryption options: In earlier versions, the WHONET encryption routine created a confidential identification number 10 characters in length. There are two new options to give you more flexibility in determining how the identifier is encrypted: 1. the length of the identifier (the default is still ten characters) and 2. the use of a “Secret word” to incorporate into the encryption algorithm (the default is with no “Secret word”). Automated encryption: For several years, WHONET has been able to run “macros” from the Windows command line. This has been a useful feature for scheduling and automation of WHONET analyses. Now, WHONET “encryption” can also be run from the Windows command line. This facilitates the automated encryption of WHONET data files. The syntax of the command is: c:\whonet5\whonet.exe encrypt file1.xxx file2.xxx In this example “file1.xxx” is the name of the original WHONET data file. “file2.xxx” is a copy of this file, but with confidential patient information removed or encrypted. Data analysis One-per-patient options: WHONET has multiple options for handling repeat options – by isolate, by patient, by time interval or resistance phenotype with a number of options for defining “time interval” and “resistance phenotype”. All of these options were available for analyses which calculate %RIS statistics. In previous versions of WHONET, only “by isolate” and “by patient – first isolate only” were possible for the other analysis options. In WHONET 5.4, this limitation has been removed, so you can choose time intervals and phenotype definitions for isolate listings and resistance profiles, for example. Organism groups: Previously, WHONET had seven pre-defined “organism groups” available for analysis: “All organisms”, “All Gram-positive organisms”, “All anaerobes”, etc. This list has been significantly extended to include options such as as “All Enterobacteriaceae”, “All non-fermenting Gram-negative rods”, “Klebsiella sp.”, “Salmonella sp.”, etc. To access these organisms, enter “Organisms” in Data analysis. Click on “Organism groups” to view the possible groupings. Note that organism group codes are given with capitalized letters. For example, use “SAL” to analyze all Salmonella isolates (S. Enteritidis, S. Typhi, Salmonella sp., etc.), but use “sal” to analyze only those isolates entered by the user as “sal” (Salmonella sp.). Blank organisms: In previous versions of WHONET, the data analysis program ignored any record in which the organism column was blank. In WHONET 5.4, WHONET will also include these results if the user selects analyses of “All organisms”. This will be especially useful for laboratories which download from an existing laboratory system if an empty field is used to indicate “No growth” instead of special code, such as “xxx” in WHONET. Isolate listing - isolate alerts: The use can view the microbiological alerts described above as additional comments in the isolate. To use this feature, go to “Analysis type”, “Isolate listing and summary”, and click on the option “Include isolate alerts”. By clicking on “Alerts”, the user may configure which alerts to use in the analysis. %RIS and test measurements by summary variables – In earlier versions of WHONET, users could choose a “Row variable” to stratify statistics for the %RIS Summary analysis (for example Specimen year, Department, Laboratory). This feature has now been added to the standard %RIS detailed report. The output thus permits an easy direct comparison of all %RIS statistics across values of the Row variable. In addition to the %R, %I, %S statistics, the output also includes the full disk diffusion zone diameters and MIC values permitting a direct comparison of the quantitative test measurements across values of the Row variable. This format – sometimes known as “Squashed histograms” – represents a numeric version of multiple histograms in one table. ESBL statistics: ESBL statistics are now included in analyses in the same manner as betalactamase results. Resistance profiles: For resistance profile summaries, the default column variable is “Specimen date”. You can choose any other variable to use instead. In previous versions of WHONET, if you selected “Age” as the column variable, WHONET would display results for precise ages: 3 months, 16 years old, 72 years old, etc. In this version, the analysis will display age groups: <1, 1-10, 11-20, etc. In a future version of WHONET, the definition of the age groups will be up to the user. BacTrack – isolate alerts: This is an enhanced version of the older WHONET analysis called “Unusual isolates”. This analysis is very similar to the “isolate listing” just described. But instead of providing a list of results for all isolates, WHONET will show only isolates which have trigger a statistical or microbiological alert. In addition to the listing of “alert” isolates, WHONET also provides a summary of the microbiological alerts triggered – how many isolates satisfy each alert, as a total and also by laboratory. By clicking on “Alerts”, the user may configure which alerts should be used in the analysis. SaTScan – SaTScan™ is a freeware software for the detection of clusters and outbreaks in public health data, developed under the joint auspices of Martin Kulldorf, of the National Cancer Institute and of Farzad Mostarashi at the New York City Department of Health and Mental Hygiene. “SaTScan” stands for “Space and Time Scan”. The software permits a number of types of statistical models for cluster detection which should prove extremely valuable in the early detection of hospital or community outbreaks of infectious organisms. WHONET is now able to call SaTScan from the Data Analysis and Quick Analysis programs, integrating the statistical results into the standard WHONET output for Isolate listing summaries and Resistance profile summaries. There is also an additional analysis option called SaTScan which displays information on the potential clusters identified by the statistical algorithms. SaTScan has both a user interface version (SaTScan.exe) and a batch command line option (SaTScanBatch.exe). Only the batch command line option is distributed with WHONET. To download the full software and documentation, go to www.satscan.org. Expert interpretation rules: By default, WHONET does not apply the expert interpretation rules for MRSA, ESBL-producers, etc. in the interpretation of results for data analysis. To apply these expert interpretation rules, the user must go to “Options” and click on “Use expert interpretation rules”. Excel: WHONET 5 has always had the ability to export results to Microsoft Excel. However, the format of this export was not very attractive. “Copy” and “Paste” was much faster and also provided better formatting in Excel. In WHONET 5.4, the Excel export has been much improved. If the user saves analysis results as “Excel”, the output in Excel is well formatted, including all relevant charts. An advantage of using Excel charts is that the user may use Excel later to modify the format and presentation of graphical data (fonts, colors, text, etc.) (WHONET graphs can be copied and pasted to other softwares, but after pasting the graph, it cannot be subsequently edited.) Macros: A macro is a small file that you can create which saves the details of any analysis that you perform. By using macros, you can facilitate the performance of commonly used analyses. You can also prepare macros for use by people who are not very familiar with data analysis with WHONET. To create a macro, select all of the analysis options that you want (analysis type, organisms, data files, etc.). Then click on “Macros”, “New”. Enter a name for the macro and click “Save”. The macro is now ready for use anytime. To use the macro at a later time, open WHONET, enter “Data analysis”, “Macros”. Find the macro that you want, and select “Load”. You can then use “Begin analysis” to start the analysis, or you can make any changes to the parameters before running the analysis. (Note: the macro feature of WHONET has not changed significantly since WHONET 5.3. It is described again here because of its expanded use in WHONET 5.4 for the new analysis feature “Quick analysis” described below.) Note: macros can be run from the Windows command line with the syntax: c:\whonet5\whonet.exe xxxx.mcr where “xxxx.mcr” is the name of the macro to run. Quick analysis reports: WHONET has a new feature called “Quick analysis” with two aims: 1. for people who are very familiar with WHONET, “Quick analysis reports” can be significant time saver for commonly performed analyses; 2. for people who are not familiar with WHONET, “Quick analysis reports” can provide a lot of valuable information quickly without the need to learn WHONET. WHONET has two kinds of reports: 1. WHONET-defined reports; and 2. user-defined reports. WHONET 5.4 has two pre-defined reports: “WHONET Standard report” and “SaTScan Space and Time Scan analysis for clusters. The first report provides a useful summary of various types of information about the data file (file overview, data completeness, invalid records, ...), laboratory configuration (problems with antibiotic breakpoints, disk potencies, ....), and microbiological findings (most common organisms, key drug-bug combinations, isolate alerts, ...). The SaTScan report provides information about possible organism outbreaks. A user-defined report consists of any number of macros created by the user in data analysis. For example, if there are five analyses that the user performs on a weekly basis, the user can create five macros using data analysis, and then put these five macros into a user-defined report. The user may wish to create separate reports for different target groups – pharmacy, infection control, hospital administration, etc. A report can also include other reports or WHONET reference tables in order to build a larger summary report. Note: reports can be run from the Windows command line with the syntax: c:\whonet5\whonet.exe xxxx.rpt where “xxxx.rpt” is the name of the report to run. BacLink Additional interface formats: Interfaces for the following systems has been added: Meditech Magic, Cerner Classic, WinPath, and the Oman Hospital Information System. In addition, BacLink can now interface with any version of Microsoft Access. In previous versions of BacLink, only Access 2 and 97 were supported. Results must be saved in the form of a “flat” non-relational table. WHONET 5.3 Update notes, December 2003 General Code lists: Additional codes have been introduced into the WHONET lists of organisms, specimen types, data fields, and antibiotics. The NCCLS 2003 breakpoints have been included. Configuration Antibiotics: There is now no limit to the number of antibiotics tests which may be included in a laboratory configuration. This will be of particular value to national surveillance data managers. Previously, a laboratory was permitted up to 105 different antibiotic tests. Due to limitations imposed by Microsoft, there is still a limit to the number of antibiotics which may appear in a single data file (limit now approximately 120), but there is no limit to the number of antibiotics which can be analyzed. Antibiotic panels: New organism panel groups have been included: Non-fermenters, Salmonella, and Campylobacter, The panel “Gram-positive” has now been separated into Staphylococcus, Streptococcus, and Streptotoccus viridans. (The existing panels S. pneumoniae and Enterococcus have not been modified.) Isolate listing field configuration: The user may now indicate which fields should be included by default in isolate listings. Previously, WHONET defined the default fields, and the user could select “Show hidden columns” to see the rest. Data entry and file management Modify clinical report: Previously, WHONET defined the contents of the patient clinical report. The user now has the ability to choose the fields to be included, fonts, headers, and footers, as well as the number of reports to include on one page. This option should be particularly useful to laboratories which utilize WHONET to print clinical reports for distribution to clinicians. The feature can be accessed from “Data entry”, “Modify clinical report”. Printing clinical reports: Previously, the user could use “Date of data entry” to select which reports to print out. Now the user can choose from one of several options: specimen number, specimen date, patient name, location, organism, etc. Modify data file structure: In a manner similar to Micrsoft Access, users may now directly edit the structure of existing data files: delete fields, rename fields, change field lengths. This feature can be accessed from “Data entry”, “Modify data file structure”. Data analysis Macros – storing analysis parameters: WHONET now has a “Macro” feature to store analysis selections and options set by the user. A macro can be useful in retrieving long lists of frequently analyzed organisms or data files, setting user preferences for option settings, saving complex search criteria, etc. Macros – automated analyses: Utilizing the Windows Task Scheduler (usually installed automatically in all versions of Windows 98 and up), it is possible to schedule analyses to run on a periodic basis, for example every Monday morning at 6 a.m. Isolate listing: As indicated above, the user may now define which fields to include by default in isolate listings. %RIS and histograms, number of isolates: The user may print out either the percentage or the number of isolates resistant, intermediate, and susceptible to an antibiotic. Number of isolates will also appear in the histogram. %RIS and histograms, combine disk, MIC, and Etest results. Previously, WHONET would always display separately results obtained from different test methods, for example gentamicindisk %resistant and gentamicin-MIC %resistant. As an option, the user can now combine these into a single statistic. This option should be useful for laboratories and countrise in which both methods are commonly used. Histograms and scatterplots, MIC ranges. Previously, MIC ranges were always displayed in graphs from 0.002 to 2048 mcg/ml. This may now be modified by the user to improve the visual display of the results. BacLink Automated data conversions: BacLink can be run in an automated fashion so that data file conversions can be scheduled, for example every Monday morning at 5:30. WHONET 5.2 Update notes General Languages: Greek and Italian have been added. The current list of languages is: English, Bulgarian, Chinese (Simplified), Estonian, French, German, Greek, Indonesian, Italian, Japanese, Norwegian (Bokmal), Norwegian (Nynorsk), Portuguese, Russian, Spanish, and Thai. If you would like to translate WHONET into your language, please let us know and we will provide instructions. Translation is most commonly accomplished by editing a text file in Excel. Configuration Data fields: The major improvement between WHONET 5.2 and WHONET 5.1 is greater flexibility in the management of data fields. WHONET now permits you to add, remove, or modify nearly all standard and optional data fields. For example, in earlier versions of WHONET, it was not possible to remove or modify fields such as "Last name" or "Date of birth". Now it is possible to remove fields which you do not use or to change the field width if you need more space. Data entry and file management Data entry screen: The placement of fields on the data entry screen is now configurable by the user. Combined with the improved flexibility in the configuration of data fields, users can now design the data entry screen into the format which they find most convenient for their work. Data files: WHONET now permits the user to delete fields from a data file that are no longer needed. Data analysis Isolate listing summary: The user may now select the data fields to be used for both the rows and the columns. In previous versions, it was only possible to select the row variable. So for example, it is now possible to summarize "Laboratory" by "Organism" or "Specimen type" by "Department". If you summarize a variable by a date variable (for example by "Specimen date"), you may now choose between a summary "by month" or "by year". Resistance profile summary: The user many now select the data field to use for the column variable. In previous versions, profiles were always summarized by specimen month and by location. Data summaries can be done by month or by year. BacTrack: The "Unusual isolates" feature has been enabled. This feature allows the automatic detection of "Unusual isolates" as defined by frequency. For example, "K. pneumoniae" susceptible to "ampicillin" is considered unusual because such strains happen less than 5% of the time. (The percentage criteria is configurable by the user.) To take advantage of this feature, you must first use "Build dictionary" to create a summary of your historical data. BacLink Data fields: The improved flexibility in data field management described for WHONET 5.2 has also been incorporated into BacLink. BacLink is thus a much more generic and flexible data interface tool for converting many kind of data between file formats. Additional interface formats: Additional instrument and LIS formats have been added, including Aura, Osiris, MIC 2000, MADS, and several formats specific for Japanese systems. Improvements in some instrument interfaces have also been made. WHONET 5.1 Update notes, June 2001 General Multilingual: The user may choose between a number of available languages. The software also permits interested users to translate the software into additional languages. Improved screen displays: Many of the screen displays have been modified to reflect new features, improved messages, and space requirements for the additional languages. File folders: Program and data files have been placed into separate folders (Codes, Data, Docs, Language, Output, Machines) to improve the organization of WHONET files. Fonts: The user can control the fonts used in screen displays and in printouts. This is especially useful for languages which do not use the Latin alphabet. New codes: New antibiotics, organisms, specimen types, patient departments, location types, and optional data fields have been defined. Veterinary, food, and environmental laboratories: A number of features have been introduced for use by non-clinical laboratories. Most of these features are in an evaluation phase, and some have been disabled in this distribution version of WHONET 5.1. BacLink 2: BacLink 2 (for Windows) is now available and is included and integrated with WHONET 5.1. BacLink can be used to transfer data from existing softwares, information systems, and automated susceptibility test instruments, avoiding the need for direct data entry into WHONET 5.1. BacLink 2 is also available in a number of languages. Removal of software bugs, clarification of instructions, improvements in software design: With the suggestions, comments, experience, and patience of many users around the world, errors have been removed from the program, confusing instructions have been reworded, displays have been redesigned. Configuration Antibiotics: The NCCLS (US), CA-SFM (French), and BSAC (UK) breakpoints and quality control ranges have been updated, and the list of antibiotics has been expanded. To update your breakpoints to the latest values, select your laboratory, choose “Modify laboratory”, “Antibiotics”, “Breakpoints”, “Update breakpoints”. After you update the breakpoints, it is essential that you print out and review the breakpoints to ensure that they are complete and correct. Before updating the breakpoints, you may wish to make a copy of the current laboratory configuration (utilizing “Copy laboratory”) in case you periodically want to use the previous antibiotic breakpoints. Copy laboratory: A “Copy laboratory” feature has been included. This may be useful in a multicentric collaboration in which all laboratories have similar antibiotic and location configurations. It can also be used to make backup copies of your laboratory configuration prior to making significant changes, for example updating the antibiotic breakpoints. After a laboratory has been created through “Copy laboratory”, the new laboratory may be edited independently of the original. Create a laboratory from a data file: This feature may be particularly useful to a national data manager. One application is the generation of a configuration for laboratories which send data to national coordinators, but forget to send the corresponding configuration file. Another application is the generation of a “national laboratory” configuration which includes a list of all antibiotics tested within the network. This “national laboratory” can be utilized to analyze easily all data files collected by the network. To take advantage of this feature, create a sample “national” data file by combining examples of data files from each of the participating laboratories. Data entry and file management Editing of the data table: Under ‘View Database’, it is now possible to directly edit records in a table format using ‘Edit table’ without retrieving each isolate individually with ‘Edit isolate’. Multiple organisms from individual patients or specimens: When saving results, the program now permits the user to enter several organisms for individual patients or specimens. This will decrease the amount of repetitive data entry required. Combining and exporting data files: Multiple files may now be easily combined. (In previous versions, only two files could be combined at a time.) An export utility has been added so that files can be exported to formats other than the usual dBASE format used by WHONET files. Encrypt patient information: This utility permits the creation of copies of your data files in which confidential patient identifier information has been removed (patient name, day of birth) or encrypted (patient number, specimen number). Month and year of birth are retained. Quality control ranges: Quality control organisms and ranges have been entered into WHONET, permitting the immediate alert of out-of-range results. Salmonella serovars: In addition to 140 Salmonella entries in the standard organism list of threeletter codes, the complete list of named serovars has been entered into WHONET. To access the complete list, you must include “Serotype” as one of your data fields. To do this, select your laboratory, select “Modify laboratory”, select “Data fields”, select “Microbiology”, select “Serotype”. Data analysis Analysis options: Additional options have been defined. For example: histograms utilizing the quality control breakpoints, scatterplots with regression lines, isolate listings with encrypted patient information and/or with BacTrack comments. One per patient: In addition to previous options, resistance estimates may now be calculated incorporating definitions of episodes (using time intervals between specimens) and/or resistance phenotypes (using antibiotic test results). Data file selection: Multiple files may be selected at the same time. Previously it was necessary to select each file individually. Clear selections: “Clear organism list” and “Clear file list” options permit the quick removal of the previous selections. Copy graph and Copy table: These options permit the simple transfer of graphs and tables to other Windows softwares, such as Microsoft Excel or Microsoft Word, using standard “cut and paste” features. Such softwares can be very valuable in the formatting of presentation quality reports and distribution materials. Sorting of analysis results: For data displayed in table format, you may click any grid heading to sort the data by that field. Click once for sorting in ascending sequence. Click twice for descending sequence.