Curriculum Vitae
David Shiuan
Address
Department of Life Science and Institute of Biotechnology
National Dong Hwa University, Hualien 974, Taiwan, ROC
Tel: 03-8633644, 8633632;
Fax: 03-8633630; E-mail: shiuan@mail.ndhu.edu.tw
Personal
Birth Date and Place: Oct 15, 1949 at Hualien, Taiwan, ROC
Citizenship: ROC and USA
Family: married (wife, Yen-Li Yang) with four children (Young, Lily, Amy, and Rom)
Education
Tamkang University (Taipei, Taiwan), BS in Chemistry 1972. 6.
State University of New York at Stony Brook (USA), PhD in Chemistry, 1981. 2.
Experience
Research Associate, State University of New York at Stony Brook, School of Medicine, Division of hypertension and
nephrology, 1981. 2. - 1983. 8.
Associate Professor, National Yang Ming Medical College, Department of Physiology, 1983. 8. - 1984. 8.
Postdoctoral Fellow, Stanford University, Department of Biological Science, 1984.8. - 1987. 8.
Scientist, Cetus Corp. at Emeryville, CA, Department of Microbial Genetics, 1987.8. - 1988. 8.
Research Scientist, Development Center for Biotechnology at Taipei, Division of Molecular Biology, 1988. 8. – 1991. 8.
Associate Professor, National Sun Yat-Sen University, Department of Biological Science, 1991.8. - 1995. 2.
Professor, National Sun Yat-Sen University, Department of Biological Science, 1995. 2. - 2001. 2.
Chief, Section of R & D, University Biotechnology Center, National Sun Yat-Sen University, 1994. 8. - 2001. 2.
Professor, National Dong Hwa University, Department of Life Science and Institute of Biotechnology, 2001. 2.– now
Chair, Department of Life Science; Director, Institute of Biotechnology; Director, Multidisciplinary Program of
Bioinformatics, National Dong Hwa University, 2004. 2.- 2009. 1.
Visiting Professor, Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland,
OR, USA 2009. 2. - 2010. 1.
1
Research Interest
Biochemistry and Molecular Biology, Bioinformatics and Virtual Screening
Professional Memberships
Taiwan Biochemical Society; Taiwan Proteomics Society; American Society for Microbiology; International
Organization for Mycoplasmologist
Publication List
A. SCI Journal
42. Lin, C. Y., Lu, M. C., Su, J. H., Chu, C. L., Shiuan, D., Weng, C. F., Sung, P. J., Huang, K.J.. 2013.
Immunomodulatory effect of marine cembrane-type diterpenoids on dendritic cells. Mar. Drugs 11(4):1336-1350.
41. Xu, L., Huang, K. J., Ho, T. S., Liu, C. C., Lee, Y. R., Lin, C. Y., Shiuan, D., Jiang, X. H. 2013. Monoclonal
antibodies for diagnosis of enterovirus 71. Monoclon. Antib. Immunodiagn. Immunother. 32(6):386-394.
40. Lin, C. Y., Lu, M. C., Su,J. H., Chu, C. L., Shiuan, D., Weng, C. F., Sung, P. J., and Huang*, K. J. 2013.
Immunomodulatory effects of marine membrane-type diterpenoids on dendritic cells. Marine Drugs (accepted)
39. Huang, K. J., Lin, S. H., Lin, M. R., Ku, H., Szkaradek, N., Marona, H., Hsu, A., and Shiuan*, D. 2013. Xanthone
Derivatives can be potential antibiotics - virtual screening for the inhibitors of enzyme I of bacterial
phosphoenolpyruvate-dependent phosphotransferase system. J. Antibiotics 66(8):453-458.
38. Lin, K. C., Chen, C. Y., Huang, K. J., Chang, C. W.,
Lin, S. P., Chang, D. K., Lin, M. R., and Shiuan*, D. 2012. .A
dodecapeptide targeting the histidine-containing phosphocarrier protein HPr of phosphoenolpyruvate
phosphotransferase system exhibits bactericidal effect and induces cell aggregation in Escherichia coli. Appl.
Microbiol. Biotechnol. 94:755-762. [SCI, 3.288]
37. Liu, W. S., Lin, P. C., Chang, L .F., Harn, H. J., Shiuan, D., Chiou, T. W., Jeng, J. R. 2011. Inhibitory effect of
n-butylidenephthalide on neointimal hyperplasia in balloon injured rat carotid artery. Phytother Res. 10:1494-1502.
[SCI, 1.878]
36. Li, Y. Z., Ho, Y. P., Chen, S. T., and Shiuan*, D. 2010. Proteomic analysis of the interactions between Mycoplasma
hyopneumoniae and porcine tracheal ciliated cells. Appl. Biochem. Biotech. 160:2248-2255. [SCI, 1.256]
35. Li, Y. Z., Ho, Y. P., Lin, C. Y., Chen, S. T., Chiou, T. W., Li, Z. S. and Shiuan* , D. 2009. Proteomic comparative
analysis of the pathogenic strain 232 and the avirulent strain J of Mycoplasma hyopneumoniae. Biochemistry
(Moscow) 74:215-220. [SCI, 1.368]
34. Chang, L.J., Chen, W. H., Minion, F. C. and Shiuan*, D. 2008. Mycoplasmas regulate the expression of heat shock
protein genes through CIRCE-HrcA interactions. Biochem. Biophys. Res. Comm. 367:213-218. [SCI, 2.855]
33. Lin, K. C., Wey, M. T., Kan, L. S. and Shiuan* , D. 2008. Characterization of the interactions of lysozyme with DNA
by surface plasmon resonance and circular dichroism spectroscopy. Appl. Biochem. Biotechnol. 158:631-641. [SCI,
1.256]
32. Wang, S. N., Liu, T. R., Chen, Y. L. and Shiuan*, D. 2007. Protective effects of a freeze-dried extract of vegetables
and fruits on the hydroxyl radical-mediated damage of DNA and decrease of erythrocyte deformability. Appl.
Biochem. Biotech.141:241-249. [SCI, 1.102]
2
31. Leong, M., Chen, C. P., Shar, K. C. and Shiuan*, D. 2007. Selection and characterization of lipase abzyme from
phage displayed antibody libraries. Biochem. Biphys. Res. Comm. 361:567-573. [SCI, 2.855]
30. Hong, Y. R., Chen, Y. L., Farh, L., Yang, W. J., Liao, C. H. and Shiuan*, D. 2006. Recombinant Candida utilis for the
production of biotin. Appl. Microbiol. Biotech. 71:211-221. [SCI, 2.034]
29. Yang, W. J., Lai, J. F., Peng, K. C., Chiang, H. J., Weng, J. N. and Shiuan*, D. 2005. Epitope mapping of Mycoplasma
hyopneumoniae using phage displayed peptide libraries and the immune responses of the selected phagotopes. J.
Immunol. Methods. 304: 15-29 [SCI, 2.744]
28. Chen, Y. S., Shiuan, D., Chen, S. C., Chye, S. M. and Chen*, Y. L. 2003. Recombinant truncated flagellin of
Burkholderia pseudomalli as a molecular probe for diagnosis of Meliodosis. Clin. Diagn Lab. Immunol. 10:423-425.
[SCI, 1.724]
27. Yang, W. J. and Shiuan*, D. 2003. Plaque reduction neutralization test (PRNT): an alternative
method to assess specific antibody response to PIII displayed peptide of filamentous. J. Immunol. Methods
276:175-183. [SCI, 2.744]
26. Chen, Y. L., Wang, S. N., Yang, W. J., Chen, Y. J. and Shiuan*, D. 2003. Expression and immunogenicity of
Mycoplasma hyopneumoniae heat shock protein antigen P42 by DNA vaccination. Infect. Immun. 71: 1155-1160.
[SCI, 4.033]
25. Farh, L., Huang, S. Y., Steinrauf, L., Chiang, H. J. and Shiuan*, D. 2001. The structure-function studies of
Escherichia coli biotin synthase via chemical modification and site-directed mutagenesis approach. J. Biochem.
(Tokyo) 130: 627-635. [SCI, 2.292]
24. Chung, T. L., Farh, L., Chen, Y. L. and Shiuan*, D. 2000. Molecular cloning and characterization of a unique 60
kDa/72 kDa antigen gene encoding the enzyme I of phosphoenolpyruvate:sugar phosphotransferase system (PTS) of
Mycoplasma hyopneumoniae. J. Biochem. (Tokyo) 128: 261-269. [SCI, 2.292]
23. Steinrauf*, L. K., Shiuan, D., Yang, W. J. and Chiang, M. 1999. Lysozyme association of nucleic acids.
Biochem.
Biophys. Res. Comm. 266: 366-370. [SCI, 2.904]
22. Steinrauf#, L. K., Chiang, M. and Shiuan, D. 1999. Molecular structure of the amyloid-forming protein kappa I Bre. J.
Biochem. (Tokyo) 125: 422-429. [SCI, 2.292]
21. Hwang, S. Y., Su, V., Farh, L. and Shiuan*, D. 1999. Bioassay of biotin concentration with an Escherichia coli bio
deletion mutant. J. Biochem. Biophys. Methods 39: 111-114. [SCI, 1.302]
20. Chang, C. W., Liao, Y. K., Su, V., Farh, L. and Shiuan*, D. 1998. Nucleotide sequencing of S-RNA segment and
sequence analysis of the nucleocapsid protein gene of the newly isolated Akabane virus PT-17 strain. Biochem. Mol.
Biol. Intl. 45: 979-987. [SCI, 1.302]
19. Wu, C. H., Bao, Y. Y., Shao, C. P. and Shiuan*, .D. 1997. Molecular cloning and nucleotide sequencing of BioF
(7-keto-8-amino pelargonic acid synthetase), BioC and BioD (dethiobiotin synthetase) genes of Erwinia herbicola.
Biochem. Mol. Biol. Intl. 41: 311-315. [SCI, 1.302]
3
18. Chou, S. Y., Chung, T. L., Chen, R. J., Ro, L. H., Tsui, P. I. and Shiuan*, D.. 1997. Molecular cloning and sequence
analysis of a 42 kDa protein gene of Mycoplasma hyopneumonaie. Biochem. Mol. Bio. Intl. 41: 821-831. [SCI,
1.302]
17. Liu, S. K., Tseng, J. N., Shiuan, D., and Hannawalt*, P. 1997. Isolation and characterization of E. coli mutants show
preferential cis-like lacZ mutagenesis: Mutation in mutS can lead to localized mutagenesis. Mol. Gen. Genet. 255:
449-459. [SCI, 2.871]
16. Shiuan*, D., Wu, C. H., Chang, Y. S. and R. J. Chang. 1997. A competitive enzyme-linked immunosorbent assay for
biotin. Methods Enzymol. 279: 321-326. [SCI, 2.312]
15. Chang, Y. S. and Shiuan*, D. 1997. Construction and characterization of a biotin regulated gene expression system in
Escherichia coli. Appl. Biochem. Biotech. 66: 49-59. [SCI, 1.132]
14. Wu, C. H., Chen, H. Y. and Shiuan*, D. 1996. Isolation and characterization of Erwinia herbicola biotin operon and
the nucleotide sequences of bioA and bioB genes. Gene 174: 251-258. [SCI, 2.871]
13. Lin, K. C. and Shiuan*, D. 1995. A simple method for DNaseI footprinting analysis. J. Biochem. Biophys. Methods
30: 85-89. [SCI, 1.302]
12. Chang, Y. S., Wu, C. H., Chang R. J. and Shiuan*, D. 1994. Determination of biotin concentration by a competitive
ELISA method. J. Biochem. Biophys. Methods 29: 321-329. [SCI, 1.302]
11. Ger, Y. M., Chen, S. L., Chang, H. J. and Shiuan*, D. 1994. A single Ser-180 mutation desensitized the feedback
inhibition of DAHP synthetase in Escherichia coli. J. Biochem. (Tokyo) 116: 986-990. [SCI, 2.292]
10. Ro, L. H., Chen, R. J. and Shiuan*, D. 1994. Rapid and efficient purification of antiserum against Mycoplasma
hyopneumoniae. J. Biochem. Biophys. Methods 28: 155-159. [SCI, 1.302]
9. Shiuan*, D., Lin, K. C. and Campbell, A. 1994. Transcriptional analysis of Escherichia coli biotin operon. Gene 145:
1-7. [SCI, 2.871]
8. Lin, K. C. and Shiuan*, D. 1993. DNaseI footprinting studies of Escherichia coli biotin repressor-operator complex. J.
Biochem. (Tokyo) 114: 670-676. [SCI, 2.292]
7. Lin, K. C., Campbell, A. and Shiuan*, D. 1991. Binding characteristics of Escherichia coli biotin repressor-operator
complex. Biochem. Biophys. Acta 1090: 317-325. [SCI, 4.447]
6. Shiuan, D. and Campbell*, A. 1988. Transcriptional regulation and gene arrangement of Escherichia coli, Citrobacter
freundii and Salmonella typhimurium biotin operons. Gene 67: 203-211. [SCI, 2.871]
5. Shiuan, D. and Weinstein*, S. 1984. Evidence for an electroneutral chloride transport pathway in brush border
membrane vesicles isolated from rabbit renal cortex. Amer. J. Phys. 247: F837-F847. [SCI, 4.416]
4. Tu*, S. I., Shiuan, D. and Marecek, J. F. 1981. Effects of fluorescamine modification on light-induced proton
movement in reconstituted purple membrane of Halobacteria halobium. Biochem. Biophys. Res. Comm. 99: 584-588.
[SCI, 3.102]
3. Ramirez*, F., Shiuan, D., Tu, S. I. And Marecek, J. F. 1980. Differential effects on energy transduction by
fluorescamine derivatives in rat liver mitochondria. Biochemistry 19: 1928-1933. [SCI, 4.008]
2. Lam, E., Shiuan, D. and Tu*, S. I. 1980. Differential inhibition of respiration and the dependent proton extrusion by
fluorescamine in rat liver mitochondria. Arch. Biochem. Biophys. 201: 330-338. [SCI, 3.152]
1. Shiuan, D. and Tu*, S. I. 1978. Fluorescent labeling of mitoplast membrane. Effect of oxidative phosphorylation
4
uncouplers. Biochemistry 7: 2249-2252. [SCI, 4.008]
B. Patents
1. US Patent 7033814 B2. Methods for preparing yeast with improved biotin productivity using integrating
plasmids encoding biotin synthase. (valid 2006 – 2022)
C. Conference Paper 20061. David Shiuan*, Yuan-Zuo Li, Yen-Peng Ho, Chih-Yuan Lin, and Shui-Tein Chen Investigating the interactions
between Mycoplasma hyopneumoniae and porcine tracheal ciliated cells by a proteomic approach. The 20th Protein
Society Symposium, Aug 5-9, 2006. San Diego, USA.
2. Leong, M., Chen, C. P., Shar, K. C. and Shiuan*, D. Selection and characterization of lipase abzyme from phage
displayed antibody libraries. The 19th FAOBMB Seoul Conference. May 27-30, 2007. Seoul, Korea.
3. Shiuan, D., Lin K. C., and Kan, L. S. Characterization of the interactions of lysozyme with DNA by surface plasmon
resonance and circular dichroism spectroscopy. Keystone Symposium of Infectious Diseases. Jan 20-25, 2008,
Keystone, Colorado, USA.
4. Shiuan, D. and Wu, S. L. Virtual screening for the cholesterol biosynthesis inhibitors. 2 nd International Conference on
Drug Discovery and Therapy, Feb 1-4, 2010. Dubai, AUE. (*Invited Speaker)
5. Shiuan, D. * and Lin, S. H. Virtual screening and selection of inhibitors against enzyme I of bacterial
phosphoenolpyruvate-dependent phosphotransferase system. 8th BioPharma India Convention, Drug Discovery
Conference, Dec 6-8, 2010, Mumbai, India. (*Invited Speaker)
6. Shiuan*, Shih-Hung Lin, Kuo-Jien Huang, Meei-Ru Lin and Hao Ku, Novel Antibiotics through Virtual Screening for
Inhibitors of Bacterial PTSI. 2012 AFOB Symposium. Feb 9-11, 2012; Hu-Chih-Min City, Vietnam. (*Invited
Speaker)
7. Kuo-Chih Lin, Chih-Yuan Chen, Chih-Wei Chang, Kuo-Jien Huang, Shih-Pin Lin, Shih-Hung Lin, Din-Kuo Chang ,
Meei-Ru Lin and David Shiuan*. A dodecapeptide (YQVTQSKVMSHR) exhibits antibacterial effect and induces cell
aggregation in Escherichia coli. The 15th International Biotechnology Symposium, Sep 16-21, 2012, Daegu, Korea.
(Oral presentation)
8. Lin, Shih-Hung and Shiuan, D. Structure-based prediction of inhibitors against HMG-CoA reductase of human
cholesterol biosynthesis pathway. The 15th International Biotechnology Symposium, Sep 16-21, 2012, Daegu, Korea.
(poster presentation).
9. Shiuan*, D., Lin, S. H. and Weng, C. F. Curcumin Has a new function - inhibitor of human HMG CoA reductase
Asian Congress of Biotechnology (ACB 2013) Dec 15-19, 2013, New Delhi, India (oral presentation).
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