Dr. Tania Nolan
Scientific Reputation
Throughout my research career I have experienced a wide range of scientific applications specialising in molecular biology.
These include clinical diagnostics, investigations into cell cycle and cellular differentiation using fundamental yeast cell cycle
biology and studies of the genetic alterations throughout the progression of breast cancer using clinical material and data.
I am a significant co-authorship of the specialist textbook “The A - Z of Quantitative PCR” (ed S.A. Bustin; IUL Press). The
publication of this book has resulted in an invitation to edit another academic text, co-author a second book, author several
book chapters and to contribute peer reviews for scientific publications.
I have been considered equal alongside prestigious academic researchers and was invited to present a plenary lecture and chair
the QPCR workshops organised by Michael Pfaffl, an organisational and decision making role for all three QPCR conferences
held in London and organised by Stephen Bustin. In addition I take a major role in organising the QPCR workshops held at
EMBL in Heidelberg (2004 to 2007) and in Prague (2005).
Career and Achievements
2004-present
Development Manager
Sigma Aldrich
I manage two important pan European initiatives; the antigenic peptide and specialist oligonucleotide design services I have
guided the development of these roles to include a European seminar series to be presented by these team members.
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2003 - 2004
Senior Field Application Scientist QPCR
Stratagene
On promotion to senior scientist I became responsible for recruiting and mentoring a team of scientists to work across Europe.
A major achievement was the conception and development of the distance learning programme now adopted as the customer
training model. In addition I managed beta tests trial for FullVelocity and Multiplex QPCR mixes which were taken through
into development and sales and developed sales tools to support local account managers.
2001 – 2003
Field Application Scientist QPCR
Stratagene
I was responsible for presale support, installation and customer training and post sale support of Stratagene QPCR portfolio in
Europe. I was also a member of the project team that managed the development of the second generation QPCR system (launch
2003). Activities included international lectures, organisation of QPCR meetings and troubleshooting workshops to provide
advanced QPCR customer support (2002). I also collaborated on development of international lecture and practical QPCR
workshops with Stephen Bustin and a on sample concentration normalisation project
1996 – 2001
Post Doctoral Research Fellow
Manchester University/AstraZeneca
I worked in the team of Prof John Hickman (Manchester University) and Prof Nigel Bundred (Manchester University/Patterson
Institute) and alongside Dr Ged Brady and Dr Philip Hedge (AstraZeneca) to develop novel screening methods to investigate
gene expression regulating apoptosis in breast cancer. I also instigated a collaboration with Dr Colin Watts in Sydney, Australia
which resulted in a publication with his group.
In addition to research activities I managed a laboratory research team of students and technicians and the related project
budget and was awarded an NIH travel grant to fund attendance to Advances in Breast Cancer Research, Lake Tahoe, USA.
I began commercial links as a consultant for Techne (UK) during the development of Quantica QPCR machine.
1993 – 1996
PhD Post Graduate Programme
Manchester University
Thesis “Molecular analysis of cell cycle genes in Candida albicans” supervised by Dr John Rosamond and examined by Dr
Paul Clarke and Prof Neil Gow. Invited speaker at a British Yeast Group meeting Norwich, UK
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Selected Publications
Quantification of mRNA using the real-time reverse transcription polymerase reaction
Tania Nolan, Rebecca E Hands, Stephen A Bustin
Nature Protocols 2006
SPUD: and assay for the detection of inhibitors in nucleic acid preparations
Tania Nolan, Rebecca E Hands, William Ogunkolade, Stephen A Bustin
(Analytical Biochemistry, 2006)
Simultaneous Detection and Sub-typing Of Human Papillomavirus In The Cervix Using Real-time Quantitative PCR
Rashmi Seth, Tania Nolan, John Rippin and David Jenkins (in preparation, 2006)
Quantitative real-time RT-PCR--a perspective.
Bustin SA, Benes V, Nolan T, Pfaffl MW. J Mol Endocrinol (2005).
Lacey HA, Nolan T, Greenwood SL, Glazier JD, Sibley CP. Gestational profile of Na+/H+ exchanger and Cl-/HCO3- anion
exchanger mRNA expression in placenta using real-time QPCR. Placenta. 2005 Jan;26(1):93-8.
The Importance of Assay Optimisation for Achieving Reliable Real Time Quantitative PCR Results
Tania Nolan, Richard Pembrey and Natalie Simpson. Origins (2005)
Pitfalls of Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction.
Stephen A Bustin and Tania Nolan. JBT (2004)
Co-author and major co-editor role for the textbook: “A-Z of Quantitative PCR” International University Line, Biotechnology
Series La Jolla, CA. (2003)
Analysis of mRNA expression by real-time PCR in Real-time PCR – An Essential Guide. Horizon Scientific Press
Stephen A Bustin and Tania Nolan (2003)
Photoperiod differentially regulates circadian oscillators in central and peripheral tissues of the Syrian hamster.
Amanda-Jayne Carr, Jonathon Johnston, Andrei Semikodskii, Tania Nolan, Felino Cagampang, J. Anne Stirland, Andrew S.I.
Loudon Curr Biol (2003)
Bcl-2 inhibits glucocorticoid transactivation: a possible mechanism for inhibition of apoptosis.
David Ray, Rosalind Gunby, Tania Nolan and Gerard Brady. Mol. Endo. (2003)
EDD, the human orthologue of the hyperplastic discs tumour suppressor gene, is amplified and overexpressed in cancer.
J.L.Clancy, M.J.Henderson, A.J. Russell, D.W.Anderson, R.J. Bova, I.G. Campbell, D.Y.H Choong, G.A. Macdonald, G.J.
Mann, T. Nolan et al Colin K.W. Watts. Oncogene (2003)
Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.
Tania Nolan and Bas Hulshof. BTi (2002).
Coexpression of parathyroid hormone related protein and its receptor in early breast cancer predicts poor patient survival.
Linforth, R., Anderson, N., Hoey, R., Nolan, T., Downey, S., Brady, G., Ashcroft, L., and Bundred, N. Clin Cancer (2002).
Global cDNA amplification combined with real-time RT-PCR: accurate quantification of multiple human potassium channel
genes at the single cell level.
Al-Taher, A., Bashein, A, Nolan T, Hollingsworth M. and Brady G. Yeast (2000).
“Isolation and molecular characterisation of the POL3 gene from Candida albicans”.
Tania Nolan and John Rosamond. Gene (1996).
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Contact Details
54, Cambridge Road,
Stretham,
Cambridgeshire,
CB6 3LR,
GB
+44 1353 648 518 (home); +44 7789 968 782 (mobile)
TaniaNolandr@AOL.com
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