Project No. and Title: NCERA057 Swine Reproductive Physiology
Period Covered: 10-2007 to 11-2008
Date of Report: 11-Aug-2008
Annual Meeting Dates: 25-Jun-2008 to 26-Jun-2008
Participants
John Parrish [parrish@ansci.wisc.edu] Wisconsin
Dave Guthrie [dave.guthrie@ars.usda.gov] USDA-BARC
Matt Wilson [Wilson@mail.wvu.edu] West Virginia
Lloyd L Anderson [llanders@iastate.edu] Iowa
Timothy J. Safranski [SafranskiT@missouri.edu] Missouri
Joe Ford [ford@email.marc.usda.gov] USDA-MARC
Rebecca Krisher [rkrisher@illinois.edu] Illinois
Duane Davis [ddavis@oznet.ksu.edu] Kansas
Mark Diekman [mdiekman@purdue.edu] Indiana
Brett White [bwhit1@unlnotes.unl.edu] Nebraska
John Baker [baker@anr.msu.edu] Michigan
Brief Summary of Minutes
The annual meeting of the NCERA-57 was called to order by Mark Diekman, local host,
at 8:15 AM on Wednesday June 25th. Mark introduced Chuck Hibberd, Associate Dean
& Director of Cooperative Extension Service. Chuck welcomed us to campus and gave
us a brief update on the Purdue University Extension Service and how he was working
towards a focus on Transformation Extension Programs. The goal is to “raise the bar”
from level 1 ‘one-on-one contacts’ to level 4 ‘transformational extension programs’.
Mark then introduced Alan Grant, Department Head of Animal Science. Alan spoke
about recent trends in animal agriculture in Indiana and the Governor’s goals for
increasing animal agriculture in Indiana. He indicated that in the face of a 30% decrease
in pig numbers in the last 17 years, the number of feeder pigs had increased 1000%
indicating a shift in the nature of the swine industry in the state. He also indicated that
over the same time period the undergraduate enrollment in Animal Science had increased
from ~200 in 1990 to ~600 undergraduate students in 2008. Discussion ensued about the
demographics and interests of those students and that similar trends have been observed
at other stations.
John Baker, Associate Director of the Michigan Agricultural Experiment Station and
Administrative Advisor to NCERA-57, addressed the need to begin the process of
rewriting the NCERA-57 project. He indicated that he could give editorial rights to three
individuals. We codified the previously discussed concept that John Parrish would chair
the rewrite committee and that Matt Wilson and Dave Guthrie would fill out the
committee. John distributed a handout containing pertinent information for the rewrite
effort and the deadlines for the various components of the renewal. He emphasized the
need for impact statements and commended our project for their success, including the
submission and planned resubmission of an integrated grant proposal to USDA- NRICGP
and the biennial symposium.
Station reports then began. Duane Davis spoke about the online lab class that he has
been developing at Kansas State to replace their wet lab for online students. He indicated
that aspects of this would fit into the teaching component of the integrated project
resubmission. One of his significant questions was how to best use modules for on and
off campus. Station reports continued until about 11 at which time we travelled to JBS
United in Sheridan, IN for the symposium organized by the regional project on Sow
Longevity.
The business meeting was called to order on June 26th. David Miller was unanimously
elected the next Secretary for NCERA-57. West Virginia University was selected as the
host institution for the next annual meeting and dates were discussed. If acceptable, the
group settled on the 24th and 25th of June, 2009 with June 23rd serving as a travel day.
Plans for the next symposium were initiated and a tentative topic of gilt development
from prenatal to mating was arrived at. John Parrish asked that all members send him
brief impact statements that he could use to initiate the renewal process. Finally, the
regional committee thanked Mark Diekman and Purdue University for graciously hosting
the annual meeting and letters of thanks will be sent to Mark and to JBS United for
hosting and supporting the biennial symposium.
Dave Guthrie spoke briefly about the grant resubmission. He indicated that the two main
issues were the proposed dose of the synchronization compound and the need for
teaching and economic analyses. He also indicated that he would again be soliciting
letters of support.
Station reports then resumed with reports from Bret White, Lloyd Anderson, Tim
Safranski, John Parrish, Joe Ford and Rebecca Krisher.
The meeting was adjourned at 11:00 and the gavel was virtually passed from John Parrish
to Dave Guthrie.
Respectfully submitted Matthew Wilson, Secretary, NCERA-57
Accomplishments
1. Dave Guthrie and Duane Davis revised the integrated proposal entitled ‘Seasonal
Infertility in Swine: Research and Educational Goals to Enhance the Profitability
and Sustainability of the Swine Industry’ that was not funded in 2007,
unfortunately it was not submitted in time for the 2007 grant cycle. Work
continues to proceed relative to additional refinement of the proposal and it
should be resubmitted in 2008.
2. The committee organized and hosted its’ biennial symposium entitled ‘Sow
Longevity’. The focus of the symposium was to extend the efforts of the
committee and others to extension faculty, producers and individuals affiliated
with the swine industry. Greater that 60 attendees pack the conference room at
JBS United for what was an excellent series of presentations and discussion. The
slides presented will be put together on to CD’s that will be distributed to the
committee, extension agents and interested individuals. The presentations should
also be available via the website that John Parrish continues to develop to serve as
a repository of information and tools for and from the committee.
3. A topic was identified for the next symposium that will focus on animal
management prenatally through breeding.
Impacts
1. Modules have been developed that would help institutions transition from wet
laboratories to online laboratories for courses in animal reproduction, including
swine reproduction.
2. Greater understanding of the window of opportunity for utilizing frozen semen for
fixed-time AI has been delineated. Efforts continue to develop better
cryopreservation of sperm for AI.
3. The genetic variation in the GnRH promoter amongst control, selected for
ovulation rate and Meishan pigs may help describe variation in the reproductive
efficiency of these breeds/lines
4. A greater understanding of the function of the porosome in the regulated secretion
of proteinacious material indicates that water leads to swelling of the porosome
and that aquaporins regulate the influx of water that is needed.
5. Fourier harmonic analysis has been refined to better describe fertility of a semen
sample. Specifically, two components of the fourier harmonics allow a very high
level of predictability as to whether a particular boar will have acceptable or
unacceptable fertility.
Publications
Sagirkaya H, Misirlioglu M, Kaya A, First NL, Parrish JJ, Memili E. Developmental
potential of bovine oocytes cultured in different maturation and
culture conditions. Anim Reprod Sci. 2007 Oct;101(3-4):225-40.
Jerde TJ, Mellon WS, Bjorling DE, Checura CM, Owusu-Ofori K, Parrish JJ,
Nakada SY. Stretch induction of cyclooxygenase-2 expression in human urothelial
cells is calcium- and protein kinase C zeta-dependent. Mol Pharmacol. 2008
Jan;73(1):18-26.
Siddiqui MA, Bhattacharjee J, Das ZC, Islam MM, Islam MA, Haque MA, Parrish
JJ, Shamsuddin M. Crossbred bull selection for bigger scrotum and shorter age at
puberty with potentials for better quality semen. Reprod Domest Anim. 2008
Feb;43(1):74-9.
K.L. Willenburg, K.J. Rozenboom and J.J. Parrish. The relationship between sperm
nuclear shape and boar fertility using fourier harmonics. Journal of Animal Science,
Volume 86, E-Supplement 2, 2008. p 525.
J. A. Sterle and J. J. Parrish. Changes that have occurred in animal science teaching.
Journal of Animal Science, 2008. Volume 86, E-Supplement 2, p 345.
J. J. Parrish. The use of podcasts in the classroom. Journal of Animal Science,
Volume 86, E-Supplement 2, 2008. p 346.
J.J. Parrish, C. Mueller, E. Ludwig and J. L. Susko-Parrish. Day length does not
affect live sperm nuclear shape in the stallion. Reproduction, Fertility and
Development. 2008 20:192.
Lefric Enwall and John Parrish. Effect of scrotal insulation on bull sperm in vitro
fertility, nuclear shape and apoptotic markers. Journal of Andrology Supplement,
Proceedings of the American Society of Andrology 33rd Annual meeting. 2008. p 47.
Antelman, J., G. Manandhar, Y.-J. Yi, R. Li, K. Whitworth, J.A. Green, R.S. Prather,
P. Sutovsky. 2008. Expression of mitochondrial transcription factor A (TFAM)
during porcine gametogenesis and preimplantation embryo development. J. Cellular
Physiology. (accepted 6-3-08).
Bonk, A.J., H.-T. Cheong, R. Li, L. Lai, Y. Hao, Z. Liu, M. Samuel, E.A. Fergason,
K.M. Whitworth, C.N. Murphy, E. Antoniou, R.S. Prather. 2007. Correlation of
developmental differences to the methylation profiles of nuclear transfer donor cells.
Epigenetics 2:3, 179-186.
Bonk, A.J., R. Li, L. Lai, Y. Hao, Z. Liu, M. Samuel, E.A. Fergason, K.M.
Whitworth, C.N. Murphy, E. Antoniou, R.S. Prather. 2008. Aberrant DNA
methylation in porcine in vitro-, parthenogenetic-, and somatic cell nuclear transferproduced embryos. Mol. Reprod. Dev. 75:250-264.
Caamaño, J.N., A. Rodriguez, A. Salas, M. Muñoz, C. Diez, R.S. Prather, E. Goméz.
2008. Flow cytometric cell cycle analysis of cultured brown bear fibroblasts. Cell
Biology International (accepted).
Hao, Y., C. Murphy, L. Spate, D. Wax, Z. Zhong, M. Samuel, N. Mathialagan, H.
Schatten, R.S. Prather. 2008. Osteopontin improves in vitro development of
porcine embryos and decreases apoptosis. Mol. Reprod. Dev. 75:291-298.
Im, G.-S., M. Samuel, L. Lai, Y. Hao, R.S. Prather. 2007. Development and
calcium level changes in pre-implantation porcine nuclear transfer embryos activated
with 6-DMAP after fusion. Mol. Reprod. Dev. 74:1158-1164.
Isom, S.C., R.S. Prather, E.B. Rucker III. 2007. Heat stress-induced apoptosis in
porcine in vitro fertilized and parthenogenetic preimplantation-stage embryos. Mol.
Reprod. Dev. 74:574-581.
Jiang, L., P. Jobst, L. Lai, M. Samuel, D. Ayers, R.S. Prather, X.C. Tian. 2007.
Expression of growth-regulating imprinted genes in deceased and surviving cloned
pigs.
Cloning and Stem Cells 9:97-106.
Jiang, L., L. Lai, M. Samuel, R.S. Prather, X. Yang, X.C. Tian. 2008. Expression
of X-linked genes in deceased neonates and surviving cloned female pigs. Mol.
Reprod. Dev. 75:265-273.
Klassen, H., K. Warfvinge, P.H. Schwartz, J.F. Kiilgaard, N. Shamie, C. Zhang, M.
Samuel, E. Scherfig, R.S. Prather, M.J. Young. 2008. Isolation of multiple
progenitor cell types from GFP-transgenic pigs and transplantation to the retina of
allorecipients. Cloning & Stem Cells (in press).
Li, R., K. Whitworth, L. Lai, D. Wax, L. Spate, C.N. Murphy, A. Rieke, S.C. Isom,
Y. Hao, Z. Zhong, M. Katayama, H. Schatten, R.S. Prather. 2007. Concentration
and composition of free amino acids and osmolalities of porcine oviductal and uterine
fluid and their effects on development of porcine parthenogenetic and IVF embryos.
Mol. Reprod. Dev. 74:1228-1235.
Liu, Z., T. Jiangtian, Z. Zhong, W. Zhenkun, S. Jun, Y. Zhi, G. Li, M. Haikun, K.
Qingran, S. Shuang, L. Yutian, W. Hongbin, R.S. Prather. 2008. Green fluorescent
protein (GFP) transgenic pig produced by somatic cell nuclear transfer. Chinese
Science Bulletin. 53:1035-1039.
Lorson, M.A., L. Spate, K. Wells, R.S. Prather, C.L. Lorson. 2008. The
identification and characterization of the porcine (sus scrofa) survival motor neuron
(SMN1) gene. Developmental Dynamics (in press).
Lovercamp, KW, TJ Safranski, KA Fischer, G Manandhar, M Sutovsky, WO Herring
and P Sutovsky. 2007. High resolution light microscopic evaluation of boar semen
quality sperm cytoplasmic droplet retention in relationship with boar fertility
parameters. Archives of Andrology, 53: 219-228.
Lovercamp KW, Safranski TJ, Fischer KA, Manandhar G, Sutovsky M, Herring W,
Sutovsky P. 2007. Arachidonate 15-lipoxygenase and ubiquitin as fertility markers in
boars. Theriogenology, Theriogenology, 67: 704-718.
Martin, L.M., C. Besch-Williford, L. Lai, H.-T. Cheong, G.-S. Im, K.-W. Park, C.
Murphy, D. Keisler, M. Ellersieck, H. Schatten, J.A. Green, R.S. Prather. 2007.
Morphologic and histologic comparisons between in vivo and nuclear transfer derived
porcine embryos.
Mol. Reprod. Dev. 74:952-960.
McHughes, C.E., G.K. Springer, L. Spate, R. Li, R. Woods, M. Green, S.W. Korte,
C.N. Murphy, J.A. Green, R.S. Prather. 2008. Identification and quantification of
differentially represented transcripts in preimplantation bovine embryos. Mol.
Reprod. Dev. (accepted 3-13-08).
Rogers, C.S., Y. Hao, T. Rokhlina, M. Samuel, D.A. Stoltz, Y. Li, E. Petroff, D.W.
Vermeer, A.C. Kabel, Z. Yan, L. Spate, D. Wax, C.N. Murphy, A. Rieke, K.
Whitworth, M.L. Linville, S.W. Korte, J.F. Engelhardt, M.J. Welsh, R.S. Prather.
2008. Production of CFTR null and ΔF508 heterozygous pigs by AAV-mediated
gene targeting and somatic cell nuclear transfer. Journal of Clinical Investigation
118:1571-1577.
Ross, J.W., M.D. Ashworth, F.J. White, G.A. Johnson, P.J. Ayoubi, U. E. DeSilva,
K.M. Whitworth, J.A. Green, R.S. Prather, R.D. Geisert. 2007. Identification and
analysis of molecular markers following endocrine disruption of pregnancy in the pig.
Endocrinology 148:4761-4773.
Wu, A.C.H., P. Sutovsky, G. Manandhar, W. Xu, M. Katayama, B.N. Day, Q.-W.
Park, R.S. Prather, R. Oko. 2007. PAWP, A sperm specific WW-domain binding
protein involved in pronuclear development during mammalian fertilization. Journal
of Biological Chemistry 282:12,164-12,175.
Yi, Y.J., G. Manandhar, M. Sutovsky, R. Li, V. Jonakova, R. Oko, C.S. Park, R.S.
Prather, P. Sutovsky. 2007. Ubiquitin C-Terminal hydrolase-activity is involved in
sperm acrosomal function and anti-polyspermy defense. Biol. Reprod. 77:780-793.
Zhong, Z., L. Spate, Y. Hao, R. Li, L. Lai, M. Katayama, D. Wax, K Whitworth, Q.Y. Sun, R.S. Prather, H. Schatten. 2007. Remodeling of centrosomes in
intraspecies and interspecies nuclear transfer porcine embryos. Cell Cycle 6:15101521.
Baltes-Breitwisch, M.M., R.C. Bott, D.M. Larson, J.M. Martin, and A.S. Cupp.
2008. Male biostimulation prior to a short melengesterol acetate (MGA)
synchronization protocol increased timed AI conception rates in multiparous cows. J.
Anim. Sci. (Submitted).
Bass, B.E., R.A. Cederberg, G.A. Mills, C.M. Clay, and B.R. White. 2008.
Responsiveness of GnRH receptor and gonadotropin subunit genes to GnRH in lines
of swine divergent for ovulation rate. Biol. Reprod. (Submitted).
Bott, R.C., D.T. Clopton, and A.S. Cupp. 2008. A proposed role for VEGF isoforms
in sex-specific vasculature development in the gonad. Reprod. Dom. Anim. (In
Press).
Bott, R.C., D.T. Clopton, R.M. McFee, and AS Cupp. 2008. Kinase Domain
Receptor (Kdr) expression is present in sex specific vascular patterns in the
developing gonads: Role for VEGF in regulation of testis specific vasculature and
spermatogenesis. Mol. Reprod. Dev. (Submitted).
Cederberg, R.A., E.A. McDonald, C. Lee, R.A. Friedrich, A.R. Cropp, and B.R.
Wh
-1 cells is partially
conferred by a distal swine upstream promoter enhancing region (SUPER) and two
proximal steroidogenic factor-1 binding sites. Endocrinology (Submitted).
Cherrington, B.D., T.A. Farmerie, B.E. Bass, R.A. Cederberg, C.M. Clay, and B.R.
White. 2008. Emergence of a new regulatory element in the proximal promoter of
the gonadotropin releasing hormone receptor in Chinese Meishan swine. Biol.
Reprod. (Accepted).
Harris, H.L., A.S. Cupp, A.J. Roberts, and R.N. Funston. 2007. Altered reproductive
response in beef heifers fed soybeans during pubertal development. J. Anim. Sci.
(Accepted).
Martin, J.L., A.S. Cupp, R.J. Rasby, Z.C. Hall, and R.N. Funston. 2007. Utilization
of dried distillers grains for developing beef heifers. J. Anim. Sci. 85:2298-2303.
Martin, J.L., D.T. Clopton, H.L. Stroh, A.S. Cupp, and R.N. Funston. 2008. Effects
of soybeans or dried distillers grains in beef heifer development diets on reproductive
performance and granulosa VEGF isoform expression. J. Anim. Sci. (Submitted).
McDonald, E.A., R.A. Cederberg, J.E. Smith, and B.R. White. 2008. Divergent
activity of the porcine GnRH receptor gene promoter in
-1 cells is partially
conferred by nuclear factor (NF)-like, and GATA4
elements. Endocrinology (Submitted).
Montagner, M.M, A.R. Cropp, J.J. Swanson, R.A. Cederberg, Paulo B.D. Goncalves,
and B.R. White. 2008. Role of gonadotropin-releasing hormone on mouse
preimplantation embryonic development. Mol. Reprod. Dev. (In Preparation).
Pohlmann, R.M., R.A. Ten Broeck, R.M. McFee, D.T. Clopton, R.C. Bott, R.A.
Longfellow, T.G. Rozell, and A.S. Cupp. 2008. Inhibition of vascular endothelial
growth factor (VEGF) signal transduction blocks follicle progression to the pre-antral
stage but does not necessarily affect vascular development in perinatal rat ovaries.
Endocrinology (Submitted).
Silva, C., J.R. Wood, L. Salvador, I. Kostetskii, C.J. Williams, and J.F. Strauss III.
2008. Expression profile of male germ cell-associated genes in mouse embryonic
stem cell cultures treated with all-trans retinoic acid and testosterone. Mol. Reprod.
Dev. (In Press).
Ten Broeck, R.A., R.M. Pohlmann, R.A. Longfellow, M.M. Baltes, D.T. Clopton,
and A.S. Cupp. 2008. Neutralization of Vascular Endothelial Growth Factor
inhibitory isoforms is a more effective mechanism than treatment with angiogenic
isoforms to stimulate vascular development and follicle progression in the perinatal
rat ovary. Endocrinology (Submitted).
Wang, H., M. Ogawa, J.R. Wood, M.S. Bartolomei, M.D. Sammel, J.P. Kusanovic,
S.W. Walsh, R. Romero, and J.F. Strauss III. 2008. Genetic and epigenetic
mechanisms combine to control MMP1 expression and its association with preterm
premature rupture of membranes. Hum. Mol. Genet. 17:1087-1096.
Wood, J.R., D.A. Dumesic, D.H. Abbott, and J.F. Strauss III. 2007. Molecular
abnormalities in oocytes from women with polycystic ovary syndrome revealed by
microarray analysis. J. Clin. Endocrinol. Metab, 92:705-713.
Zhang, Z., R. Jaimez, X. Shen, D. Gude, H. Tang, J.R. Wood, E. Goldberg, and J.F.
Strauss III. 2008. Autoregulation of SPAG16 expression: A single gene encoding an
axoneme structural protein and a transcription factor that activates the axoneme
protein promoter. Nucleic Acids Res. (Submitted).
Albin DM, Wubben JE, Rowlett JM, Tappenden KA, Nowak RA. 2007. Small
intestinal transport and barrier function respond differently to acute immune
challenge in two porcine breeds. Journal of Animal Science, 85:2517-2523.
Bahr JM 2008 Are scientific societies serving the needs of graduate and postdoctoral
students. Reproduction, Fertility and Development 20:19-22.
Bahr JM. The chicken as a model organism. In: Source Book of Models for
Biomedical Research, M. Conn (ed). Humana Press, pp. 161-167, 2008.
Barua A, Abramowicz J, Bahr JM, Bitterman P, Dirks A, Holub KA, Bradaric MJ,
Edassery SL, and Luborsky JL. 2007. Detection of ovarian tumors in chicken by
ultrasonography: a step towards early diagnosis in humans? J of Ultrasound and
Medicine 26:909-919.
Barua A, Edassery SL, Bitterman P, Abramowicz JS, Dirks AL, Bahr JM, Hales DB,
Bradaric MJ, Luborsky JL 2008 Prevalence of anti-tumor antibodies in laying hen
model of human ovarian cancer. International J. of Gynecol. Cancer. (in press).
Belton Jr. RB, Chen L, Mesquita FS, Nowak RA. 2008. Basigin-2 is a cell surface
receptor for soluble basigin ligand. J Biol Chem [Epub April 22, 2008].
Bleck GT, MB Wheeler, LB Hansen, H Chester-Jones, and DJ Miller. 2008. Lactose
in milk of cows from several breeds at various stages of lactation. Reprod Domestic
Animals. In Press.
Boltz CR, Boltz DA, Bunick D, Scherba G, Bahr JM. 2007. Vaccination against the
avian infectious bronchitis virus affects sperm concentration, sperm quality, and
blood testosterone concentrations in cockerels. British Poultry Science 48;617-624.
Braundmeier AG, WG Breed, and DJ Miller. 2008. Spermatozoa from a marsupisal,
-galactosyltransferase. Reprod Fertilit Develop.
20:402-407.
Chen L, Nakai M, Belton Jr. R J, and Nowak RA. 2007. Expression of extracellular
matrix metalloproteinase inducer and matrix metalloproteinases during mouse
embryonic development. Reproduction 133:405-414.
Collins ED, WL Flowers, RD Shanks, and DJ Miller. 2008. Porcine sperm-zona
binding ability as an indicator of fertility. Anim Reprod Sci. 104:69-82.
Hales DB, Zhuge Y, Lagman JAJ, Ansenberger K, Mahon C, Barua A, Luborsky J,
Bahr JM 2008 Cyclooxygenases expression and distribution in the normal ovary and
their role in ovarian cancer in the domestic hen (Gallus Domesticus). Endocrine (in
press).
Herrick, J.R., Bond, J.B., Magarey, G.M., Bateman, H.L., Krisher, R.L., Dunford,
S.A., Swanson, W.F. (2007) Toward a feline-optimized culture medium: impact of
ions, carbohydrates, essential amino acids, vitamins, and serum on development and
metabolism of in iitro fertilization-derived feline embryos relative to embryos grown
in vivo. Biol. Reprod. 76:858-870.
Krisher, R.L., Brad, A.M., Herrick, J.R., Sparman, M.L., Swain, J.E. (2007) A
comparative analysis of metabolism and viability in porcine oocytes during in vitro
maturation. Animal Reproduction Science. 98:72-96.
Landers EA, HR Burkin, G Bleck, L Lesley Howell-Skalla, and DJ Miller. 2008.
-I sequence, mapping, and expression. Reprod
Domestic Animals. In Press.
Miles, J.R., Blomberg, L.A., Krisher, R.L., Everts, R.E., Sonstegard, T.S., Van
Tassell, C.P., Zuelke, K.A. (2008) Comparative transcriptome analysis of in vivo- and
in vitro-produced porcine blastocysts by small amplified RNA-serial analysis of gene
expression (SAR-SAGE). Molecular Reproduction and Development. 75:976-988.
Paris, M.C.J., Mastromonaco, G.F., Paris, D.B.B.P., Krisher, R.L. (2007) A
perspective on the role of emerging technologies for the propogation of companion
animals, non-domestic and endangered species. Reproduction, Fertility and
Development 19(6), iii-vii.
Stammer K, Edessary SL, Barua A, Bitterman P, Bahr JM, Hales DB, Luborsky JL
2008 Selenium binding protein 1 expression in ovaries and ovarian tumors in the
laying hen, a spontaneous model of human ovarian cancer. Gynecologic Oncology
109(Apr):115-121.
Stevenson, J.S., Tenhouse, D.E., Krisher, R.L., Lamb, G.C., Larson, J.E., Dahlen,
C.R., Pursley, J.R., Bello, N.M., Fricke, P.M., Wiltbank, M.C., Brusveen, D.J.,
Burkhart, M., Youngquist, R.S., Garverick, H.A. 2008. Detection of anovulation by
heatmount detectors and transrectal ultrasonography before treatment with
progesterone in a timed insemination protocol. Journal of Dairy Science. (Accepted)
Wheeler, M.B. 2007. Agricultural applications for transgenic livestock. Trends in
Biotechnology 25:204- 210.
Wheeler, M.B. Lima, A.S., VanEtten, T., Mello, M.R.B., 2007. Transgenic animals
for biomedicine and agriculture. In, Bovine Theriogenology, de la Sota, R.L. and Bo,
G.A., Eds. 36 pgs. (in press).
Wheeler, M.B., D. Kim, E. Monaco, W.L. Hurley 2007. Application of robotic and
microfluidic technologies to stem cell culture and analysis. Tsukuba Meeting for
Animal Biotechnology (TMAB) – Progress in Somatic Cell Nuclear Transfer
Technology, January 12-12, 2007 Tsukuba City, Japan, Pgs. 31-32.
Wheeler, M.B., Davidson, T.R., Malusky, S.A., Ferguson, C.E., Lima, A.S., Mello,
M. B. 2007. Production of transgenic livestock: Technology and applications. In:
Biotécnicas Aplicadas à Reprodução Animal, eds. P.B. Goncalves & J. Figuriedo,
(new edition in press).
Wheeler, M.B., Mello, M.R.B., Lima, A.S., VanEtten, T. 2007. Modification of the
domestic animal genome. In, Bovine Theriogenology, de la Sota, R.L. and Bo, G.A.,
Eds., 45 pgs. (in press).
Wheeler, M.B., Walters, E.M. Beebe, D. J. 2007. Toward culture of single gametes:
The development of microfluidic platforms for assisted reproduction. Theriogenology
68S:S178-S189.
Woodard, J., A. Hilldore, , M. B. Wheeler, S. Clark, S. Lan, R. Jamison, and A. J.
Wagoner Johnson, 2007. The mechanical properties and osteoconductivity of
hydroxyapatite bone scaffolds with multi-scale porosity. Biomaterials 28:45–54.
Zhao H, K Reim, N Brose, and DJ Miller. 2008. Complexin I deficient sperm are
subfertile due to defects in the acrosome reaction. Submitted.
Zhao L*, H Burkin*, X Shi, L Li, K Reim, and DJ Miller. 2007. Complexin I is
required for mammalian sperm acrosomal exocytosis. (*Both authors contributed
equally to the paper). Dev Biol 309:236-244.
Zhao L, X Shi, L Li, and DJ Miller. 2007. Dynamin 2 associates with complexins
and is found in the acrosomal region of mammalian sperm (cover feature). Mol
Reprod Dev 74:750-757.
Cassar, G., R.N. Kirkwood, M.J. Seguin, T. Widowski, A. Farzan, A.J. Zanella and
R.M. Friendship. 2008. Influence of stage of gestation at grouping and presence of
boars on farrowing rate and litter size of group-housed sows. J. Swine Health Prod.
16:81-85.
Straw, B.E., R.O. Bates and G. May. 2008. Influence of method of administration of
prostaglandin on farrowing and relationship between gestation length and piglet
performance. J. Swine Health Prod. 16:138-143.
Zak, L.J., G.R. Foxcroft, F.X. Aherne and R.N. Kirkwood. 2008. Role of luteinizing
hormone in primiparous sow responses to split weaning. Reprod. Dom. Anim. (in
press).
Manjarin, R., G. Cassar, D.J. Sprecher, R.M. Friendship, J.C. Dominguez and R.N.
Kirkwood. 2008. Effect of eCG or eCG plus hCG on estrus expression and ovulation
in prepubertal gilts. Reprod. Dom. Anim. (in press).
Manjarin, R., J.C. Dominguez, M.J. Castro, B. Vallado, S. Rodriguez, D.J. Sprecher,
G. Cassar, R.M. Friendship and R.N. Kirkwood. Effect of prior FSH treatment on the
estrus and ovulation responses to eCG in prepubertal gilts. Anim. Reprod. Sci. (in
press).
Manjarin, R., J.C. Dominguez, M.J. Castro, D.J. Sprecher, G. Cassar, R.M.
Friendship and R.N. Kirkwood. 2008. Effect of hCG treatment on the estrus and
ovulation responses to FSH in prepubertal gilts. Reprod. Dom. Anim. (in press).
Pelland, C, G. Cassar, R.N. Kirkwood and R.M. Friendship. 2008. Fertility following
intrauterine insemination with a reduced number of spermatozoa in sows with
synchronized ovulation. J. Swine Health Prod. (in press).
Cassar G, R.N. Kirkwood and R.M. Friendship. Low sow reproductive performance
related to estrus detection and timing of insemination. The Pig Journal (in press).
Lee, K, Fodor, WL, Machaty, Z. 2007. Dynamics of lamin A/C in porcine nuclear
transfer embryos. Mol. Reprod. Dev. 74:1221-1227.
Magnani, L, Cabot, R. 2007. Developmental arrest is induced when the chromatin
remodeling protein SMARCA2 in overexpressed in cleavage stage porcine embryos.
Molecular Reproduction and Development 74(10), pages 1262-1272.
Magnani, L, Cabot, R. (In Press) In vitro and in vivo derived porcine embryos
possess similar, but not identical, patterns of Oct4, Nanog and Sox2 mRNA
expression during cleavage development. Molecular Reproduction and Development.
Magnani, L, Lee, K, Fodor, W, Machaty, Z, Cabot, R. 2007. Developmental
capacity of porcine nuclear transfer embryos correlate with levels of chromatin
remodeling transcripts in donor cells. Molecular Reproduction and Development
75(5):766-76.
Nanassy, L, Lee, K, Javor, A, Machaty, Z. 2008. Effects of activation methods and
culture conditions on development of parthenogenetic porcine embryos. Anim.
Reprod. Sci. 104:264–274.
Nanassy, L, Javor, A, Machaty, Z. 2007. Changes in MPF and MAPK activities in
porcine oocytes activated by different methods. Theriogenology 68:146-152.
Sega, MF, Lee, K, Machaty, Z, Cabot, R. 2007. Pronuclear stage porcine embryos
do not possess a strict asymmetric distribution of lysine 9 dimethylation of histone H3
based solely on parental origin. Molecular Reproduction and Development 74(1):2-7.
Vajta, G, Zhang, Y, Machaty, Z. 2007. Somatic cell nuclear transfer in pig: recent
achievements and future possibilities. Reprod. Fertil. Dev. 19:403–423.
Alexander, L.J., Juneja, B., Shiroma, D., Nonneman, D., Snelling, W., Fahrenkrug,
S.C. 2007. Comparative and physical mapping of 111 previously reported and 105
new porcine microsatellites. Animal Genetics 38(6):584-594.
Archibong, A.E., Ramesh, A., Niaz, M.S., Brooks, C.M., Roberson, S.I., Lunstra,
D.D. 2008. Effects of Benzo(a)pyrene on Intra-testicular Function in F-344 Rats.
Int. J. Environ. Res. Public Health 5(1):32-40.
Bischoff, S.R., Tsai, S., Hardison, N., York, A., Freking, B.A., Nonneman, D.,
Rohrer, G., Piedrahita, J.A. 2008. Identification of SNPs and INDELS in swine
transcribed sequence using short oligonucleotide microarrays. BMC Genomics
9:252.
Casas, E., Lunstra, D.D., Cundiff, L.V., Ford, J.J. 2007. Growth and pubertal
development of F1 bulls from Hereford, Angus, Norwegian Red, Swedish Red and
White, Friesian, and Wagyu sires. Journal of Animal Science 85(11):2904-2909.
Chitko-McKown, C.G., Leonard, J.C., Moscatello, D., Miller, L.C., Freking, B.A.
2008. Development of cell lines from the sheep used to construct the CHORI-243
ovine BAC library. Animal Biotechnology 19(2):84-88.
Cushman, R.A., Allan, M.F., Thallman, R.M., Cundiff, L.V. 2007. Characterization
of biological types of cattle (Cycle VII): Influence of postpartum interval and estrous
cycle length on fertility. Journal of Animal Science 85(9):2156-2162.
Echternkamp, S.E., Thallman, R.M., Cushman, R.A., Allan, M.F., Gregory, K.E.
2007. Increased calf production in cattle selected for twin ovulations. Journal of
Animal Science 85(12):3239-3248.
Echternkamp, S.E., Cushman, R.A., Allan, M.F., Thallman, R.M., Gregory, K.E.
2007. Effects of ovulation rate and fetal number on fertility in twin-producing cattle.
Journal of Animal Science 85(12):3228-3238.
Freking, B.A., Leymaster, K.A., Vallet, J.L., Christenson, R.K. 2007. Number of
fetuses and conceptus growth throughout gestation in lines of pigs selected for
ovulation rate or uterine capacity. Journal of Animal Science 85(9):2093-2103.
Humphray, S.J., Scott, C.E., Clark, R., Marron, B., Bender, C., Camm, N., Davis, J.,
Jenks, A., Noon, A., Patel, M., Sehra, H., Yang, F., Rogatcheva, M.B., Milan, D.,
Chardon, P., Rohrer, G., Nonneman, D., de Jong, P., Meyers, S.N., Archibald, A.,
Beever, J.E., Schook, L.B., Rogers, J. 2007. A high utility integrated map of the pig
genome. Genome Biology 8(7):R139.
Kuehn, L.A., Rohrer, G.A., Nonneman, D.J., Thallman, R.M., Leymaster, K.A.
2007. Detection of single nucleotide polymorphisms associated with ultrasonic
backfat depth in a segregating Meishan x White Composite population. Journal of
Animal Science 85(5):1111-1119.
Lents, C., Heidorn, N., Barb, C., Ford, J. 2008. Central and peripheral administration
of kisspeptin activates gonadotropin but not somatotropin secretion in prepubertal
gilts. Reproduction 135(6):879-887.
Macdonald, G.J., Wise, T.H., Sluss, P.M., Ford, J.J. 2007. Breed differences in
clearance of porcine FSH in hypophysectomized rats. Animal Reproduction Science
102(3-4):328-334.
Miles, J.R., Blomberg, L.A., Krisher, R.L., Everts, R.E., Sonstegard, T.S., Van
Tassell, C.P., Zuelke, K.A. 2008. Comparative transcriptome analysis of in vivoand in vitro-produced porcine blastocysts by small amplified RNA-serial analysis of
gene expression (SAR-SAGE). Molecular Reproduction and Development
75(6):976-988.
Rohrer, G.A., Freking, B.A., Nonneman, D. 2007. Single nucleotide polymorphisms
for pig identification and parentage exclusion. Animal Genetics 38(3):253-258.
Smith, T.L., Merry, S.T., Harris, D.L., Ford, J.J., Ike, J., Archibong, A.E., Ramesh, A.
2007. Species-specific testicular and hepatic microsomal metabolism of
benzo(a)pyrene, an ubiquitous toxicant and endocrine disruptor. Toxicology in Vitro
21(4):753-758.
Vallet, J.L., Freking, B.A., Miles, J.R., Nienaber, J.A., Brown-Brandl, T.M. 2006.
Factors affecting litter size in pigs. National Swine Improvement Federation (31):1521.
Vallet, J.L., Freking, B.A. 2007. Differences in placental structure during gestation
associated with large and small pig fetuses. Journal of Animal Science 85(12):32673275.
A.E. Brazle, B. J. Johnson, S. K. Webel, T. J. Rathbun, and D. L. Davis. 2008.
Omega-3 fatty acids in the gravid pig uterus as affected by maternal supplementation
with omega-3 fatty acids. J. Anim. Sci. (in revision).
Musser, R. E., S.S. Dritz., D.L. Davis, M.D. Tokach, J.L. Nelssen, R.D. Goodband,
and K.Q. Owen. 2007. Effects of L-carnitine in the Gestating Sow Diet on Fetal
Muscle Development and Carcass Characteristics of the Offspring. J. Appl. Agric.
Res. 31:105-111.
Blomberg, L, Hashizume, K, Viebahn, C. 2008. Blastocyst elongation, trophoblastic
differentiation, and embryonic pattern formation. Reproduction. 135:181-195.
Blomberg, L. A., Schreier, L. L. and Talbot, N. C. 2008. Expression analysis of
pluripotency factors in the undifferentiated porcine inner cell mass and epiblast
during in vitro culture. (in press, accepted by Mol. Reprod. Dev. 75:450-463.
Guthrie, H. D., Long, J. A., and Welch, G. R. (accepted). Mitochondrial function and
reactive oxygen species action in relation to boar sperm motility. Theriogenology
(VIth International conference on boar semen preservation, Guelph, Ontario. August
12-15, 2007).
Guthrie, H. D. and Welch, G. R. 2008. Determination of high mitochondrial
membrane potential with the mitochondrial probe 5, 5', 6, 6'-tetrachloro-1, 1', 3, 3'tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) and for using fluorescenceactivated flow cytometry. Methods in Molecular Biology, vol. 477: Advanced
Protocols in Oxidative Stress I., D. Armstrong, ed. Humana Press Inc., Totowa, NJ.
Guthrie, H. D. and Welch, G. R. (submitted). Effect of increasing the cooling time
before boar semen freezing on fertility after a single fixed time insemination.
Guthrie, H. D. Woods III, L. C., Long, J. A. and Welch, G. R. 2008. Effects of
osmolality of a TRIS free base NaCl medium on testicular striped bass (Morone
saxatilis) sperm energy status. Theriogenology. 69:1007-1012.
Miles, J. R., Freking BA, Blomberg, L. A., Vallet, J. L., Zuelke, K. A. 2008.
Conceptus development during blastocyst elongation in lines of pigs selected for
increased uterine capacity or ovulation rate. J Anim Sci. [Epub ahead of print]
PMID: 18469062
Miles, J. R., Blomberg, L. A., Krisher, R. L., Everts, R. E., Sonstegard, T. S., Van
Tassell, C. P. and Zuelke, K. A. 2008. Comparative transcriptome analysis of in vivo
and in vitro produced porcine blastocysts by small amplified RNA serial analysis of
gene expression (SAR-SAGE). Mol Reprod Dev. 75: 976-988.
Talbot, N. C., Blomberg, L. A. The Pursuit of ES Cell Lines of Domesticated
Ungulates. Stem Cell Rev. (in press)
Talbot, N. C., Blomberg, L. A., Mahmood, A., Caperna, T. J. and Garrett, W. M.
2007. Isolation and characterization of porcine visceral endoderm cell lines from in
vivo 11-day blastocysts. In Vitro Cell. Dev. Biol. Anim. 43:72-86.
Wilson, ME, Anderson, LL. (In Press) Mechanistic aspects of foetal development
relating to postnatal health and metabolism in pigs. In: Managing Prenatal
Development to Enhance Livestock Productivity. Edited by P. Greenwood and A.
Bell. International Atomic Energy Agency, Vienna, Austria pp. 1-48.
Bobe, G, Ametaj, BN, Young, JW, Anderson, LL, Beitz, DC. 2007. Exogenous
glucagon effects on health and reproductive performance of lactating dairy cows with
mild fatty liver. Animal Reproduction Science 102:194-207.
Scanes, CG, Glavaski-Joksimovic, A, Johannsen, SA, Anderson, LL. 2007. Subpopulations of somatotropes with differing intracellular calcium concentration
responses to secretagogues. Neuroendocrinology 85:221-231. (Cover illustration).
Anderson, LL. (In Press) EDITORIAL FORWARD for ‘Cell Secretion’ Review
Series—Cell Secretion: an orchestra involving intracellular protein transport, the
universal secretory machinery-‘porosome’, and secretory vesicle swelling-induced
content release. Journal of Cellular and Molecular Medicine.
Molina, JR, Benoit, AM, Lkhagvadorj, S, Anderson, LL. (In Press) Hypothalamic
deafferentation in prepuberal beef heifers: effects of gonadotropin-releasing hormone
and estradiol benzoate on luteinizing hormone secretion. Livestock Science.
Benoit, AM, Molina, JR, Lkhagvadorj, S, Anderson, LL. (In Press) Prolactin
secretion after hypothalamic deafferentation in beef calves: response to haloperidol,
-methyl- -tyrosine, thyrotropin-releasing hormone and ovariectomy. Animal
Reproduction Science.
Anderson, LL. 2008. Invited BOOK REVIEW entitled: Force Microscopy:
Applications in Biology and Medicine. [Edited by] Bhanu P. Jena and J.K. Heinrich
Hörber. John Wiley & Sons, Inc., Hoboken, NJ; 2006, 300 pages. ISBN-13978-0471-39628-4. Microscopy and Microanalysis 14:205-206.
ANDERSON, LL, DAY, BN, FORD, SP. 2007. Robert M. Melampy: First
President of SSR. http://www.ssr.org/Melampy.shtml
Dekkers, JCM, Cai, W, Lkhagvadorj, S, Couture, O, Tuggle, CK, Nettleson, D,
Anderson, LL. 2008. Genetic aspects of Residual Feed Intake in Pigs. 41st Annual
Meeting of ASAS Midwestern Section-ADSA Midwest Branch Meeting, March 1719, 2008, Polk County Convention Center, Des Moines, IA, No. 46, p. 15.
Lee, J, Stromer, MH, Jeftinija, S, Jeftinija, K, Scanes, CG, Anderson, LL. 2007.
Immunocytochemical patterns of somatotrophs, mammotrophs, and
mammosomatotrophs in the porcine anterior pituitary. 10th International Pituitary
Congress, June 5-7, 2007, The Fairmont Hotel, Chicago, Illinois, Abstract P61, pp.
51-52. (Chicago)
Bohan, MM, Anderson, LL, Trenkle, AH, Beitz, DC. 2007. Nutrient regulation of
plasma ghrelin concentration in lean and overweight male humans. Experimental
Biology FASEB