Supplementary Figure and Table Legends (doc 34K)

advertisement
Titles and Legends to Supplementary Tables and Figure
Supplementary Table S1. CML patient sample details. WBC (G/l): white blood count (grams/liter); Hb
(g/dl): hemoglobin (grams/deciliter); Plt (G/l): platelets (grams/liter); Basos: basophils; pb: peripheral
blood; CRP (mg/dl): c-reactive protein (milligrams/deciliter); HU: hydroxyurea
Supplementary Table S2. All proteins purified on bosutinib matrix from K562 cells. Proteins are
sorted by the number of identified unique peptides (peptide counts). IPI-IDs are IPI protein database
entries to which identified peptides were assigned. Proteins listed have at least one specific peptide
that confirms the presence of the protein in the pull-down. PD1 and PD2 refer to the duplicate pulldowns performed for each cell line.
Supplementary Table S3. All proteins purified on bosutinib matrix from the accelerated phase CML
patient 1 cells. Proteins are sorted by the number of identified unique peptides (peptide counts). IPIIDs are IPI protein database entries to which identified peptides were assigned. Proteins listed have at
least one specific peptide that confirms the presence of the protein in the pull-down. PD1 and PD2
refer to the duplicate pull-downs performed for each cell line.
Supplementary Table S4. All proteins purified on bosutinib matrix from the accelerated phase CML
patient 2 cells. Proteins are sorted by the number of identified unique peptides (peptide counts). IPIIDs are IPI protein database entries to which identified peptides were assigned. Proteins listed have at
least one specific peptide that confirms the presence of the protein in the pull-down. PD1 and PD2
refer to the duplicate pull-downs performed for each cell line.
Supplementary Table S5. Identified kinase targets of bosutinib. IPI-ID indicates IPI protein database
entries to which identified peptides (AvePepCnt) were assigned. Unless otherwise indicated, kinases
present in this list have been specifically identified twice in at least one of the three cell types. The
average sequence coverage (AveSeqCov) is based on these unique peptides and takes into account
the size of the protein. If a protein was identified in only one of the two repeats per cell type, then the
values are given in italics. Where no peptide was identified to specifically confirm that a protein is
1
present, the values are given in parentheses (x). Proteins are sorted alphabetically. The BCR-ABL
fusion protein is split into the components BCR and ABL1, plus the short peptide sequence
characteristic of the fusion region of BCR-ABL. The top five hits for each cell type are highlighted in
bold. PD=Pulldown
Supplementary Table S6. Complete Millipore KinaseProfilerTM panel. For each kinase, the percent
activity remaining at 1 µM and 10 µM of bosutinib, c-bosutinib and dasatinib were measured.
Supplementary Table S7. IC50 (nM) or activity (%) remaining at 10 µM determined for selected
kinases. Kinases are of human origin unless otherwise stated.
Supplementary Figure Legend
Supplementary Figure S1. Millipore KinaseProfiler Service Assay Protocols. This manual provides
the screening conditions and protocols used in the Millipore KinaseProfiler radiometric protein kinase
assays and HTRF® lipid kinase assays.
2
Download
Related flashcards

Protein complexes

27 cards

Blood proteins

22 cards

Transport proteins

45 cards

Structural proteins

12 cards

Structural proteins

13 cards

Create Flashcards