Group 1: Distribution of benthic macroinvertebrates in glacier-fed streams Aims 1. to collect data for a range of environmental variables 2. to introduce benthic macroinvertebrate sampling methods 3. to investigate relationships between macroinvertebrates and environmental variables 4. to compare the findings with contemporary alpine stream ecosystem concepts Methods 1. Visit three sites along the main glacier-fed river, one site close to the snout, one mid-way downstream and one just before the river reaches the hydro lake. 2. At each site make detailed observations of water and air temperature, electrical conductivity, turbidity, channel morphology, pH, dissolved oxygen and stream discharge. 3. At each site collect three Surber samples for benthic macroinvertebrates 4. Back at the hut, sort the Surber samples and identify benthic macroinvertebrates using published keys. 5. Using a range of statistical methods, look for relationships between environmental variables and stream invertebrates 6. Compare results with published studies to determine how representative the glacier-fed river is with those in other parts of the world. Tasks Before starting fieldwork, think about the following questions and make some suitable hypotheses that you can later use your data to answer 1. How are the different environmental variables likely to change as we move further downstream from the glacier? 2. How are benthic macroinvertebrate metrics such as total abundance, number of taxa/species and diversity likely to change as we move further downstream from the glacier? 3. How will this glacier-fed river compare with other glacier-fed rivers in Europe and other parts of the world? Results/Presentation You are in relatively large groups so to achieve the aims of this study you will need to divide up the tasks. One of the biggest tasks is the identification of invertebrates so most people should be assigned to this in the first instance. Others may wish to analyse the environmental data while some read the background literature then communicate information to the rest of the group. The sampling strategy has been designed to provide you with data which can be analysed using a range of statistical methods commonly adopted by stream ecologists. It is your group’s responsibility to decide which statistical methods are appropriate to help you accept/reject your hypotheses. When presenting your findings, give a brief overview of the sampling strategy to familiarise other groups with what you have done. Present results as graphs/tables to clearly convey the main findings. A range of papers/book chapters are available for you to gain information from previously published studies. Remember to include appropriate referencing throughout. Group 2: Distribution of benthic macroinvertebrates in different stream types Aims 1. to collect data for a range of environmental variables 2. to introduce benthic macroinvertebrate sampling methods 3. to investigate relationships between macroinvertebrates and environmental variables in different stream types 4. to compare the findings with contemporary alpine stream ecosystem concepts Methods 1. Visit three different stream types: (1) the main glacier-fed river close to the glacier snout, (2) one groundwater stream draining valley side-slopes, and (3) one lake outlet stream. 2. At each site make detailed observations of water and air temperature, electrical conductivity, turbidity, channel morphology, pH, dissolved oxygen and stream discharge. 3. At each site collect three Surber samples for benthic macroinvertebrates 4. Back at the hut, sort the Surber samples and identify benthic macroinvertebrates using published keys. 5. Using a range of statistical methods, look for relationships between environmental variables and stream invertebrates 6. Compare results with published studies to understand the importance of different stream types in alpine basins. Tasks Before starting fieldwork, think about the following questions and make some suitable hypotheses that you can later use your data to answer 1. How are the different environmental variables likely to vary between the glacier-fed river, groundwater stream and lake outlet? 2. How are benthic macroinvertebrate metrics such as total abundance, number of taxa and diversity likely to differ between stream types? 3. How will the different stream types compare with similar stream types in other parts of Europe and the world? Results/Presentation You are in relatively large groups so to achieve the aims of this study you will need to divide up the tasks. One of the biggest tasks is the identification of invertebrates so most people should be assigned to this in the first instance. Others may wish to analyse the environmental data while some read the background literature then communicate information to the rest of the group. The sampling strategy has been designed to provide you with data which can be analysed using a range of statistical methods commonly adopted by stream ecologists. It is your group’s responsibility to decide which statistical methods are appropriate to help you accept/reject your hypotheses. When presenting your findings, give a brief overview of the sampling strategy to familiarise other groups with what you have done. Present results as graphs/tables to clearly convey the main findings. A range of papers/book chapters are available for you to gain information from previously published studies. Remember to include appropriate referencing throughout. Group 3: Temporal dynamics of glacier-fed stream ecosystems Aims 1. to collect data for a range of environmental variables 2. to introduce benthic macroinvertebrate sampling methods 3. to investigate relationships between macroinvertebrates and environmental variables in different stream types 4. to compare the findings with contemporary alpine stream ecosystem concepts Methods 1. Visit the main glacier-fed river midway downstream and remain there throughout the day 2. Every 30 minutes make detailed observations of water and air temperature, electrical conductivity, turbidity, channel morphology, pH, dissolved oxygen and river discharge. 3. Three drift nets will deployed at hourly intervals over five hours and left for 30 minutes each time to sample drifting aquatic invertebrates. 4. Back at the hut, sort the drift samples and identify benthic macroinvertebrates using published keys. 5. Using a range of statistical methods look for relationships between environmental variables and stream invertebrate drift. 6. Compare results with published studies to determine how comparable glacier-fed streams are to other river ecosystems. Tasks Before starting fieldwork, think about the following questions and make some suitable hypotheses that you can later use your data to answer 1. How are the different environmental variables likely to vary throughout the day? 2. How are metrics such as total abundance, number of taxa and diversity of drifting invertebrate likely to differ throughout the day? Results/Presentation You are in relatively large groups so to achieve the aims of this study you will need to divide up the tasks. One of the biggest tasks is the identification of invertebrates so most people should be assigned to this in the first instance. Others may wish to analyse the environmental data while some read the background literature then communicate information to the rest of the group. The sampling strategy has been designed to provide you with data which can be analysed using a range of statistical methods commonly adopted by stream ecologists. It is your group’s responsibility to decide which statistical methods are appropriate to help you accept/reject your hypotheses. When presenting your findings, give a brief overview of the sampling strategy to familiarise other groups with what you have done. Present results as graphs/tables to clearly convey the main findings. A range of papers/book chapters are available for you to gain information from previously published studies. Remember to include appropriate referencing throughout. Group 4: The role of disturbance in glacier-fed stream ecosystems Aims 1. to collect data for a range of environmental variables 2. to introduce benthic macroinvertebrate sampling methods 3. to investigate relationships between macroinvertebrates and environmental variables, and disturbance events. 4. to compare the findings with disturbance concepts and theories Methods 1. Visit the main glacier-fed river midway downstream and remain there throughout the day 2. At two 20m stream reaches make detailed observations of water and air temperature, electrical conductivity, turbidity, channel morphology, pH, dissolved oxygen and stream discharge. 2. Sample the two stream reaches for benthic macroinvertebrates by collecting three Surber samples. 3. At the bottom of the lower reach, deploy three drift nets for thirty minutes to gain an understanding of natural drift. 4. Two people enter the lower 20m reach and vigorously disturb the streambed sediments for ten minutes. During this event, at the bottom of the reach deploy three drift nets for thirty minutes to gain an understanding of disturbance induced drift. 5. Collect three Surber samples from both 20m reaches following the disturbance. 6. At the bottom of the lower reach, deploy three drift nets for thirty minutes to gain an understanding of post-disturbance natural drift. 7. Back at the hut, sort the drift samples and identify benthic macroinvertebrates using published keys 5. Using a range of statistical methods look for Before-After-Control-Impact (BACI) differences. 6. Compare results with published studies to determine to understand how glacier-fed river ecosystems respond to disturbance. Tasks Before starting fieldwork, think about the following questions and make some suitable hypotheses that you can later use your data to answer 1. How are the different environmental variables likely to vary between the two river reaches before, during and after the disturbance effect? 2. How are metrics such as total abundance, number of taxa and diversity of drifting invertebrate likely to differ before and after disturbance at the two river reaches? 3. How will the composition of drifting insects differ between the two river reaches? Results/Presentation You are in relatively large groups so to achieve the aims of this study you will need to divide up the tasks. One of the biggest tasks is the identification of invertebrates so most people should be assigned to this in the first instance. Others may wish to analyse the environmental data while some read the background literature then communicate information to the rest of the group. The sampling strategy has been designed to provide you with data which can be analysed using a range of statistical methods commonly adopted by stream ecologists. It is your group’s responsibility to decide which statistical methods are appropriate to help you accept/reject your hypotheses. When presenting your findings, give a brief overview of the sampling strategy to familiarise other groups with what you have done. Present results as graphs/tables to clearly convey the main findings. A range of papers/book chapters are available for you to gain information from previously published studies. Remember to include appropriate referencing throughout. Invertebrate identification recording sheets Site Sample Date Sample Reference Sample Processor Invertebrate taxa Date Completed Data Entered Y/N Tally Total Invertebrate identification recording sheets Site Sample Date Sample Reference Sample Processor Invertebrate taxa Date Completed Data Entered Y/N Tally Total Sharp edges and corners, plane surfaces roughened 1 Surface dull, darkened, stained by algae or minerals. Bright surfaces <5% of area Assorted sizes tightly packed or overlapping 1 4. Percent of riverbed stable 5. Scouring and deposition 6. Clinging aquatic vegetation (mosses and algae) 2. Brightness 3. Consolidation and particle size of substrate Score Score Score Component 1. Rock angularity Score Pfankuch stability score assessment criteria (Pfankuch, 1975) Rounded edges and corners. Surfaces smooth and flat Mostly dull but may have up to 35% bright surfaces, some dull on larger rocks 2 Corners and edges well rounded in 2 dimensions 3 Well rounded in all dimensions, surfaces smooth 4 2 Mixture 5050% dull and bright (±15%) 3 Predominantly bright >65% exposed or scoured surfaces 4 2 Moderately packed with some overlapping 4 6 No packing evident, loose assortment easily moved 8 80-100% 4 50-80% 8 Mostly a loose assortment with no apparent overlap 20-50% 12 0-20% 16 <5% channel length affected by scouring and deposition 6 12 30-50% affected. Deposits and scour at obstructions, constrictions at bends. Some filling in pools 18 >50% of the bottom in a state of flux or change nearly year long 24 Abundant. Growth largely moss like, dark green year round. In swift water 1 5-30% affected. Scour at constrictions and where gradient steepens. Some deposition in backwaters and pools Common. Algae forms in low velocity and pool areas. Moss in swift waters 2 Present but spotty. Mostly in backwater areas. Seasonal blooms make rocks slick 3 Perennial types 4 scarce or absent. Yellowgreen shortterm bloom may be present Total stability score = sum of the 6 components Lower scores = higher stability Site description recording sheet Sample Site Grid reference Date/Time Weather Water temperature (ºC) Air Temperature (ºC) Turbidity (NTU) Electrical conductivity (µScm-1) Stream width (m) pH Dissolved oxygen (%) Pfankuch Score Stream discharge Segment Segment Width (m) Segment depth (m) Segment velocity (m s-1) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Total discharge (Σ segment discharges) Segment discharge (m3s-1)