‫عنوان البحث‬
Changes in nitrogen content and protein profiles following in vitro selection of NaCl resistant
mung bean and tomato
‫أسماء الباحثين‬
Hassan N.M.,
Serag M.S.,
El-Feky F.M.,
‫بيانات الباحثين‬
Botany Department, Faculty of Science, Mansoura University, Damietta, Egypt
Abstract
Mung bean and tomato were in vitro selected on media containing 0, 25, 50, 100 and 150 mM NaCl. Two types
of media (hormone supplemented media, CB and hormone free media, MS) were used for mung bean using
cotyledon explants whereas two types of explants (cotyledons and shoot apices) were used for tomato on MS
media. Total-N, protein content, nitrite reductase (NiR) activity and protein profiles were checked in selected
plants and compared to original non selected ones. NaCl at low concentrations slightly increased total-N in
shoots and roots of in vitro selected mung bean and tomato whereas higher concentrations induced significant
reductions. Similar increases in protein content were detected at lower concentrations with no significant
effects thereover. On the contrary, NaCl gradually inhibited NiR activity. Similar responses of total-N, protein
and NiR activity, but with greater magnitudes, were detected in original plants. In addition, NaCl significantly
reduced dry weights of shoots and roots of either in vitro selected or, in particular, original intact plants.
Moreover, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of protein from shoots of
either in vitro selected or intact plants showed that NaCl induced new protein bands while some others were
concomitantly disappeared. The induction of one or more of the 86.4, 79, 77.6, 77 and 71.5 kDa bands
following in vitro selection and/or the disappearance of the 86 kDa band from intact plants seemed necessary
for mung bean resistance. Also, the presence of 86.2 kDa band and/or the loss of the 85.8 and 57.5 kDa bands
might be included in tomato resistance. Of these induced bands in mung bean selected on CB media, only two
bands were detected in plants selected on MS media. In tomato, two bands lost following selection from
cotyledons but only one band lost following selection from shoot apices. These changes in protein pattern
therefore might serve as adaptive regulators for resistance to NaCl.
Keywords:
In vitro selection, Lycopersicon esculentum, nitrite reductase, protein profile, resistance to NaCl, Vigna radiate
Published In:
Acta Physiol. Plant., 26, 165-175 (2004)
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