Sample(s)
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GE Healthcare - General Biacore™ Questionaire Dear customer In order to organize the workshop best it would be very helpful to have some information about your project, the nature of the molecules and previous results. The following form should help to collect the details. The file we sent is a “protected” Word document, just tab through the document to fill in the information. The file should be returned no later than 4 weeks prior to the start of the user day. Thanks for your co-operation! Contact Information Company:......................................................................................................................................... Department: ..................................................................................................................................... Head of Department:........................................................................................................................ Street: .............................................................................................................................................. Zip code: .......................................................................................................................................... City: .................................................................................................................................................. Country: ........................................................................................................................................... Date: ................................................................................................................................................ Customer: ........................................................................................................................................ Tel: ................................................................................................................................................... Fax: .................................................................................................................................................. e-mail: .............................................................................................................................................. Web page: ....................................................................................................................................... System, Project, Goal Which Biacore™ system are you using? A100 T100 X100 other (please specify) ........................................................................................................................... Please, describe your project/goal with a few words. ................................................................................................................................................................... ................................................................................................................................................................... ................................................................................................................................................................... ................................................................................................................................................................... Page 1 of 3 Ligand In Biacore terms the ligand is the molecule bound to the Sensor Chip. This can be either done by direct or chemical coupling or by capturing with another (bio)-molecule. Name: ................................................................................................. (full name and abbreviation) Origin: ........................................................................................................... (commercial or “self made”) Source:................................................................................................ Class of substance: .......................................................................... (human, mouse, etc.) (protein, virus, particles, etc.) Molecular weight: ........................................................................................................................ Da Isoelectric point: ................................................................................................................. pH units Multimer: ............................................................................................................... (yes/no/number) Details: ................................................................................................................. (SS bridges, etc.) Co-factors for activity: ............................................................................................... (e.g. Calcium) Purity: ............................................................................................................................... in percent Activity:............................................................................................................................. in percent Sample dissolved in: ........................................................................................................................ Concentration:.............................................................................................................. nM or µg/ml Amount:........................................................................................................................................ ml Stability (temperature, pH, salt, etc.): .............................................................................................. Storage conditions (temperature, pH, salt, etc.): ............................................................................. Capturing tools make use of e.g. tags on the ligand. Fusion protein, specify tag ....................................................................................... (his, GST etc.) Other labels................................................................................................................... (e.g. biotin) Sample(s) Please fill in here the properties of the interaction partner (analyte). Name: ................................................................................................. (full name and abbreviation) Origin: ........................................................................................................... Source:................................................................................................ Class of substance: .......................................................................... (human, mouse, etc.) (commercial or “self made”) (protein, virus, particles, etc.) Molecular weight: ........................................................................................................................ Isoelectric point: ................................................................................................................. Da pH units Multimer: ............................................................................................................... (yes/no/number) Details: ................................................................................................................. (SS bridges, etc.) Co-factors for activity: ............................................................................................... (e.g. Calcium) Purity: ............................................................................................................................... in percent Activity:............................................................................................................................. in percent Page 2 of 3 Sample dissolved in: ........................................................................................................................ Concentration:.............................................................................................................. nM or µg/ml Amount:........................................................................................................................................ ml Stability (temperature, pH, salt, etc.): .............................................................................................. Storage conditions (temperature, pH, salt, etc.): ............................................................................. Capturing tools make use of e.g. tags on the ligand. Fusion protein, specify tag ....................................................................................... Other labels................................................................................................................... (his, GST etc.) (e.g. biotin) Interaction conditions The binding between ligand and compound will depend on a number of conditions. Binding or running buffer has to be selected carefully. Please indicate if any information about the conditions is available. Binding buffer: .................................................................................................................................. Working temperature: ...................................................................................................................... Essential co-factors: ........................................................................................................................ Avoid this: ........................................................................................................................................ Your assays Here you can describe the assay you have used for the initial screenings and further characterizations or if you have used the ligand in affinity chromatography. ................................................................................................................................................................... ................................................................................................................................................................... ................................................................................................................................................................... Previous BIACORE Results Do you have knowledge about previous experiments with your samples on Biacore instruments Yes No ................................................................................................................................................................... ................................................................................................................................................................... Additional information you consider as useful .................................................................................................................................................................. ................................................................................................................................................................... Tuesday, February 16, 2016 ..................................................................................................... Title, name Page 3 of 3
