Sample(s)

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GE Healthcare - General Biacore™ Questionaire
Dear customer
In order to organize the workshop best it would be very helpful to have some information about your
project, the nature of the molecules and previous results. The following form should help to collect the
details.
The file we sent is a “protected” Word document, just tab through the document to fill in the information.
The file should be returned no later than 4 weeks prior to the start of the user day.
Thanks for your co-operation!
Contact Information
Company:.........................................................................................................................................
Department: .....................................................................................................................................
Head of Department:........................................................................................................................
Street: ..............................................................................................................................................
Zip code: ..........................................................................................................................................
City: ..................................................................................................................................................
Country: ...........................................................................................................................................
Date: ................................................................................................................................................
Customer: ........................................................................................................................................
Tel: ...................................................................................................................................................
Fax: ..................................................................................................................................................
e-mail: ..............................................................................................................................................
Web page: .......................................................................................................................................
System, Project, Goal
Which Biacore™ system are you using?
A100
T100
X100
other (please specify) ...........................................................................................................................
Please, describe your project/goal with a few words.
...................................................................................................................................................................
...................................................................................................................................................................
...................................................................................................................................................................
...................................................................................................................................................................
Page 1 of 3
Ligand
In Biacore terms the ligand is the molecule bound to the Sensor Chip. This can be either done by direct
or chemical coupling or by capturing with another (bio)-molecule.
Name: .................................................................................................
(full name and abbreviation)
Origin: ...........................................................................................................
(commercial or “self made”)
Source:................................................................................................
Class of substance: ..........................................................................
(human, mouse, etc.)
(protein, virus, particles, etc.)
Molecular weight: ........................................................................................................................
Da
Isoelectric point: .................................................................................................................
pH units
Multimer: ...............................................................................................................
(yes/no/number)
Details: .................................................................................................................
(SS bridges, etc.)
Co-factors for activity: ...............................................................................................
(e.g. Calcium)
Purity: ...............................................................................................................................
in percent
Activity:.............................................................................................................................
in percent
Sample dissolved in: ........................................................................................................................
Concentration:..............................................................................................................
nM or µg/ml
Amount:........................................................................................................................................
ml
Stability (temperature, pH, salt, etc.): ..............................................................................................
Storage conditions (temperature, pH, salt, etc.): .............................................................................
Capturing tools make use of e.g. tags on the ligand.
Fusion protein, specify tag .......................................................................................
(his, GST etc.)
Other labels...................................................................................................................
(e.g. biotin)
Sample(s)
Please fill in here the properties of the interaction partner (analyte).
Name: .................................................................................................
(full name and abbreviation)
Origin: ...........................................................................................................
Source:................................................................................................
Class of substance: ..........................................................................
(human, mouse, etc.)
(commercial or “self made”)
(protein, virus, particles, etc.)
Molecular weight: ........................................................................................................................
Isoelectric point: .................................................................................................................
Da
pH units
Multimer: ...............................................................................................................
(yes/no/number)
Details: .................................................................................................................
(SS bridges, etc.)
Co-factors for activity: ...............................................................................................
(e.g. Calcium)
Purity: ...............................................................................................................................
in percent
Activity:.............................................................................................................................
in percent
Page 2 of 3
Sample dissolved in: ........................................................................................................................
Concentration:..............................................................................................................
nM or µg/ml
Amount:........................................................................................................................................
ml
Stability (temperature, pH, salt, etc.): ..............................................................................................
Storage conditions (temperature, pH, salt, etc.): .............................................................................
Capturing tools make use of e.g. tags on the ligand.
Fusion protein, specify tag .......................................................................................
Other labels...................................................................................................................
(his, GST etc.)
(e.g. biotin)
Interaction conditions
The binding between ligand and compound will depend on a number of conditions. Binding or running
buffer has to be selected carefully. Please indicate if any information about the conditions is available.
Binding buffer: ..................................................................................................................................
Working temperature: ......................................................................................................................
Essential co-factors: ........................................................................................................................
Avoid this: ........................................................................................................................................
Your assays
Here you can describe the assay you have used for the initial screenings and further characterizations
or if you have used the ligand in affinity chromatography.
...................................................................................................................................................................
...................................................................................................................................................................
...................................................................................................................................................................
Previous BIACORE Results
Do you have knowledge about previous experiments with your samples on Biacore instruments
Yes
No
...................................................................................................................................................................
...................................................................................................................................................................
Additional information you consider as useful
..................................................................................................................................................................
...................................................................................................................................................................
Tuesday, February 16, 2016 ..................................................................................................... Title, name
Page 3 of 3
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