BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Lynch, Kristen Wood
Associate Professor
eRA COMMONS USER NAME
klynch
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
Harvard University
Harvard University
University of California, San Francisco
DEGREE
(if applicable)
BA
Ph.D.
YEAR(s)
1990
1996
1997-2001
FIELD OF STUDY
Biochemistry
Biochemistry
Immunology
Please refer to the application instructions in order to complete sections A, B, and C of the Biographical
Sketch.
A. Positions and Honors
Research Positions
1989-1990
Undergraduate Research
Laboratory of Prof. Mark Ptashne, Harvard University
1992-1996
Graduate Student
Laboratory of Prof. Tom Maniatis, Harvard University
1997-2001
Postdoctoral Fellow
Laboratory of Prof. Arthur Weiss, UC, San Francisco
2001-2007
Assistant Professor
Department of Biochemistry, UT Southwestern Medical Center
2007-2009
Associate Professor
Department of Biochemistry, UT Southwestern Medical Center
2009-present
Associate Professor
Department of Biochemistry and Biophysics
University of Pennsylvania School of Medicine
Honors and Awards
1991-1994
National Science Foundation Pre-doctoral Fellowship
1999-2001
Cancer Research Institute Post-doctoral Fellowship
2001-2009
E.E. and Greer Garson Fogelson Scholar in Biomedical Research
UTSWMC Endowed Scholar Program
2004-2009
National Science Foundation Career Award
B. Peer-Reviewed Publications
Topp J.D., J. Jackson, A.A. Melton, K.W. Lynch (2008) A cell-based screen for splicing regulators identifies
hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4. RNA 14: 2038-2049.
House, A.E. and K.W. Lynch (2008) Regulation of alternative splicing: more than just the ABCs. J. Biol.
Chem. 18: 1217-1221.
Melton, A.A., J. Jackson, J. Wang and K.W. Lynch. (2007) Combinatorial control of signal-induced exon
repression by hnRNP L and PSF. Mol. Cell. Biol. 27:6972-6984.
Ip, J., A. Tong, Q. Pan, J. Topp, B.J. Blencowe* and K.W. Lynch*. (2007) Global analysis of
alternative splicing during T cell activation. RNA 13: 563-572. (* co-corresponding authors)
House, A.E. and K.W. Lynch. (2006) An Exonic Splicing Silencer Represses Spliceosome Assembly
after ATP-dependent Exon Recognition. Nat. Struct. Mol. Biol. 13: 937-944.
Johnson, E.B., D.J. Steffen, K. W. Lynch, and J. Herz. (2006) Defective splicing of MEGF7/LRP4,
a regulator of distal limb development, in autosomal recessive Mulefoot disease. Genomics 88:
600-609.
Levinson, N., R. Hinman, A. Patil, C.R.J. Stephenson, S. Werner, G.H.C. Woo, J. Xiao, P. Wipf and
K.W. Lynch (2006) Use of transcriptional synergy to augment sensitivity of a splicing reporter
assay. RNA 12:925-930.
Tong, A., J. Nguyen and K.W. Lynch (2005) Differential expression of CD45 isoforms is controlled
by the combined activity of basal and inducible splicing regulatory elements in each of the variable
exons. J. Biol. Chem. 280: 38297-304.
Rothrock, C.R.*, A.E. House* and K.W. Lynch (2005) HnRNP L represses exon splicing via a
regulated exonic splicing silencer. EMBO J 24: 2792-2802. (* co-first authors)
Lynch, K.W. (2004) Consequences of regulated pre-mRNA splicing in the immune system. Nat Rev
Immunol. 4: 931-940.
Rothrock, C., B. Cannon, B. Hahm and K.W. Lynch (2003) A conserved signal-responsive sequence
mediates activation-induced alternative splicing of CD45. Mol. Cell 12: 1317-1324.
Sheives, P. and K.W. Lynch (2002) Identification of cells deficient in signaling-induced alternative
splicing by use of somatic-cell genetics. RNA 8:1473-1481.
Lynch, K.W. and A. Weiss (2001) A CD45 polymorphism associated with multiple sclerosis disrupts an
exonic splicing silencer. J. Biol. Chem. 276: 24341-24347.
Lynch, K.W. and A. Weiss (2000) A model system for activation-induced alternative splicing of CD45
pre-mRNA in T cells implicates protein kinase C and Ras. Mol Cell Biol 20: 70-80.
Hertel, K.J., K.W. Lynch and T. Maniatis (1997) Common themes in the function of transcription and
splicing enhancers. Curr Opin Cell Biol. 9: 350-357.
Hertel, K.J., K.W. Lynch, E. C. Hsiao, E. H.-T. Liu and T. Maniatis (1996) Structural and functional
conservation of the Drosophila doublesex splicing enhancer repeat elements. RNA 2: 969-981.
Lynch, K.W. and T. Maniatis (1996) Assembly of specific SR protein complexes on distinct
regulatory
elements of the Drosophila doublesex splicing enhancer. Genes & Dev. 10: 2089-2101.
Lynch, K.W. and T. Maniatis (1995) Synergistic interactions between two distinct elements of a
regulated splicing enhancer. Genes & Dev. 9: 284-293.
Sadowski, I., D. Neidbala, K. Wood and M. Ptashne (1991) Gal4 is phosphorylated as a consequence
of transcriptional activation. Proc. Natl. Acad. Sci.USA 88: 10510-10514.
Ruden, D.M., J. Ma, Y. Li, K. Wood and M. Ptashne (1991) Generating yeast transcriptional activators
containing no yeast protein sequences. Nature 350: 250-252.
C. Other Support
Current
NIH-NIGMS
Role: PI
“Mechanisms of Signal-Induced Alternative Splicing: CD45”
Duration: 5/01/08-6/30/12
This project is focused on characterizing the cis-sequences, RNA binding proteins and signaling pathways
that mediate signal-induced repression of CD45 exon 4.
NIH-NIGMS
Role: PI
“Coordination of Inducible Alternative Splicing Networks in Human T cells”
Duration: 6/01/08-7/31/12
This project is focused on characterizing sequence motifs and trans-regulatory proteins that control coregulation of alternative splicing within multiple genes in response to activation of cultured T cell lines.
Completed
NSF
Role: PI
“CAREER: Characterization of novel targets of activation-induced alternative splicing”
Duration: 3/1/04-2/28/09
This project is focused on identifying new targets and networks of signal induced splicing regulation in T
cells.
Welch Foundation
Role: PI
“Structural Characterization of a Novel Transition in Spliceosome Assembly”
Duration: 6/01/06-05/31/09
This is a small foundation award which is funding pilot studies toward a structural characterization of the AEC
complex.