劉瑞雰助理教授(Betty Revon Liu, Assistant Professor)
Research background
Cellular internalization of nanomaterials mediated by cell-penetrating peptides
Special techniques:
1). Bionanotechnology
2). Fluorescence Application
3). Cellular Bioimaging
4). Molecular Biology
Research Interests:
1). Peptides-mediated drug/biomolecules delivery system
2). Bionanomaterials
3). Biophysics
Publication：
1. Lu SW, Hu JW, Liu BR, Lee CY, Li JF, Chou JC, Lee HJ (2010). Arginine rich intracellular
delivery peptides synchronously deliver covalently and noncovalently linked proteins into plant
cells. Journal of Agricultural and Food Chemistry, 58, 2288-2294. (SCI)
2. Xu Y, Liu BR, Lee HJ, Shannon KS, Winiarz JG, Wang TC, Chiang HJ, Huang YW (2010).
Nona-arginine facilitates delivery of quantum dots into cells via multiple pathways. Journal of
Biomedicine and Biotechnology, 2010, 948543. (SCI)
3. Liu BR, Li JF, Lu SW, Lee HJ, Huang YW, Shannon KB, Aronstam RS (2010). Cellular
internalization of quantum dots noncovalently conjugated with arginine-rich cell-penetrating
peptides. Journal of Nanoscience and Nanotechnology, 10, 6534-6543. (SCI)
4. Liu BR, Huang YW, Chiang HJ, Lee HJ (2010). Cell-penetrating peptide functionized quantum
dots for intracellular delivery. Journal of Nanoscience and Nanotechnology, 10, 7897-7905.
(SCI)
5. Liu BR, Huang YW, Winiarz JG, Chiang HJ, Lee HJ (2011). Intracellular delivery of quantum
dots mediated by a histidine- and arginine-rich HR9 cell penetrating peptide through the direct
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membrane translocation mechanism. Biomaterials, 32, 3520-3537. (SCI)
Dai YH, Liu BR, Chiang HJ, Lee HJ (2011). Gene transport and expression by arginine-rich
cell-penetrating peptides in Paramecium. Gene, 489, 89-97. (SCI)
Chen YJ, Liu BR, Dai YH, Lee CY, Chan MH, Chen HH, Chiang HJ, Lee HJ (2012). A gene
delivery system for insect cells mediated by arginine-rich cell-penetrating peptides. Gene, 493,
201-210. (SCI)
Liu BR, Lin MD, Chiang HJ, Lee HJ (2012). Arginine-rich cell-penetrating peptides deliver
gene into living human cells. Gene, 505, 37-45. (SCI)
Liou JS, Liu BR, Martin AL, Huang YW, Chiang HJ, Lee HJ (2012).Protein transduction in
human cells is enhanced by cell-penetrating peptides fused with an endosomolytic HA2
sequence. Peptides, 37(2), 273–284. (SCI)
10. Liu BR, Huang YW, Chiang HJ, Lee HJ (2013). Primary effectors in the mechanisms of
transmembrane delivery of arginine-rich cell-penetrating peptides. Advanced Studies in Biology,
5(1), 11-25. (SCI)
11. Huang YW, Lee HJ, Liu BR, Chiang HJ, Wu CH (2013). Cellular internalization of quantum
dots. Methods in Molecular Biology, 991, 249-259. (SCI)
12. Liu BR, Chiang HJ, Huang YW, Chan MH, Chen HH, Lee HJ (2013).Cellular internalization of
quantum dot mediated by cell-penetrating peptides. Pharmaceutical Nanotechnology, 1,
151-161.
13. Liu BR, Huang YW, Lee HJ (2013). Mechanistic studies of intracellular delivery of proteins by
cell-penetrating peptides in cyanobacteria. BMC Microbiology, 13, 57. (SCI)
14. Liu BR, Liou JS, Huang YW, Aronstam RS, Lee HJ (2013). Intracellular delivery of
nanoparticles and DNAs by IR9 cell-penetrating peptides. PLoS One, 8:e64205. (SCI)
15. Liu BR, Winiarz JG, Moon JS, Lo SY, Huang YW, Aronstam RS, Lee HJ (2013). Synthesis,
characterization and applications of carboxylated and polyethylene-glycolated bifunctionalized
InP/ZnS quantum dots in cellular internalization mediated by cell-penetrating peptides. Colloids
and Surfaces B: Biointerfaces, 111C:162-170. (SCI)
16. Liu BR, Lo SY, Liu CC, Chyan CL, Huang YW, Aronstam RS, Lee HJ (2013). Endocytic
trafficking of nanoparticles delivered by cell-penetrating peptides comprised of nano-arginine
and a penetration accelerating sequence. PLoS One, 8(6):e67100. (SCI)
17. Liu BR, Liou JS, Chen YJ, Huang YW, Lee HJ (2013). Delivery of nucleic acids, proteins, and
nanoparticles by arginine-rich cell-penetrating peptides in rotifers. Marine Biotechnology,
15(5):584-95. (SCI)
18. Liu BR, Chen HH, Chan MH, Huang YW, Aronstam RS, Lee HJ (2015). Three Arginine-Rich
Cell-Penetrating Peptides Facilitate Cellular Internalization of Red-Emitting Quantum Dots.
Journal of Nanoscience and Nanotechnology, 15(3):2067-2078. (SCI)
19. Liu BR, Huang YW, Aronstam RS, Lee HJ (2015). Comparative Mechanisms of Protein
Transduction Mediated by Cell-Penetrating Peptides in Prokaryotes. Journal of Membrane
Biology, 248(2):355-368. (SCI)
※專書及專書論文
1). Liu BR, Huang YW, Aronstam RS, and Lee HJ. 2014 Internalization of noncovalently
associated proteins by cell-penetrating peptides in cyanobacteria. In: Cyanobacteria
Ecological Importance, Biotechnological Uses and Risk Management. Hauppauge, New
York, USA: Nova Science Publishers Inc. pp. 171-180.
2). Liu BR, Chan MH, Chen HH, Lo SY, Huang YW, Lee HJ. 2013 Protein transduction in
human cells mediated by arginine-rich cell-penetrating peptides in mixed covalent and
noncovalent manners. In: Macromolecular Chemistry: New Research. New York, USA:
Nova Science Publishers Inc. pp. 69-82.
3). Liu BR, Chan MH, Chen HH, Lo SY, Huang YW, Lee HJ. 2013 Effects of Surface Charge
and Particle Size of Cell-Penetrating Peptide/Nanoparticle Complexes on Cellular
Internalization. In: Cell Membrane: Molecular Structure, Physiochemical. (ISBN:
978-1-62808-456-6). New York, USA: Nova Science Publishers Inc.
4). Liu BR, Chiang HJ, Lee HJ. 2011 Chapter 10 Arginine-Rich Cell-Penetrating Peptides in
Cellular Internalization. In: Arginine Amino Acid. Jacobs N.L. Ed., Nova Science
Publishers Inc., Hauppauge, New York, pp. 207-218.
5). Lee CY, Liu BR, Wang YH, Hou YW, Chou JC, Lee HJ. 2011 Chapter 15 RNA
Interference Mediated by Cell-Penetrating Peptides. In: Gene Silencing: Theory,
Techniques and Applications. Catalano A.J. Ed., Nova Science Publishers Inc.,
Hauppauge, New York, pp. 335-342.
6). Liu BR, Chen CY, Chen RL, Chiang HJ, Huang YW, Lee HJ. 2010 Chapter 8
Macropinocytosis: possible mechanisms of cellular entry of arginine-rich intracellular
delivery peptides. In: Endocytosis: Structural Components, Functions and Pathways.
Dowler B.C. Ed., Nova Science Publishers Inc., Hauppauge, New York, pp. 176-190.
7). Liu BR, Hou YW, Chan MH, Chen HH, Lee HJ. 2010 Chapter 15 A novel transdermal
drug delivery system mediated by arginine-rich intracellular delivery peptides. In:
Dermatology Research Focus on Acne, Melanoma and Psoriasis. Roth D.E. Ed., Nova
Science Publishers Inc., Hauppauge, New York, pp. 281-292.
8). Lee HJ, Chou JC, Chen CP, Liu BR. 2008 DNA transfection and expression mediated by
arginine-rich intracellular delivery peptides in plant cells. In: Transgenic Plants: New
Research. Chan O.T. Ed., Nova Science Publishers Inc., Hauppauge, New York, pp.
xix-xxvi.