Activities in 2009
Classical swine fever
Professor Trevor Drew
Veterinary Laboratories Agency - Weybridge
Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, United Kingdom
Tel.: +44(0)1932 357 637, Fax: +44(0)1932 357 239
Email: t.w.drew@vla.defra.gsi.gov.uk Website: www.vla.gov.uk
Summary of general activities related to the disease
1.
Test(s) in use/or available for the specified disease at your laboratory
Test
Code
CSF Antibody ELISA *
CSF Serum neutralisation
(differential) *
TC1251
TC1254
QA
AI
Diagnostic Export Import Private Research TOTAL
80 1,393
25
24
0
2
424
4
29
0
619
0
99
0
2,644
55
1b) Virus detection
Test
Code
CSF Virus isolation*
CSF RT-PCR*
CSF Monoclonal antibody typing*
QA
TC1256
TC0592
TC0451
AI
2
0
7
Diagnostic Export Private Research TOTAL
0
0
0
0
0
360
0
0
3
0
0
176
0
0
0
2
0
546
*These tests are accredited to ISO17025 under the VLA’s United Kingdom Accreditation Service (UKAS) Testing
Laboratory 1769 certificate.
2.
Production and distribution of diagnostic reagents
Sales of diagnostic reagents
Monoclonal antibodies
CSF Mab WH211
CSF Mab WH303
Product Code
Total sold
PA0242
PA0826
12
22
Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
VLA participates annually in the EU Ring Trial organised by the OIE Reference Laboratory, Hannover, Germany.
Additionally, VLA provides a comprehensive commercial service in respect of proficiency testing. Details can be
obtained on our website at http://www.defra.gov.uk/vla/eqa/eqa-home.htm
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
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Classical swine fever
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
VLA produces strong positive, weak positive and negative International Serum Standards for CSF. Two sets of
these standards were supplied in-year.
5.
Research and development of new procedures for diagnosis and control
An extensive vaccine efficacy trial of a conventional C-strain vaccine has been completed, involving challenge
with diverse strains of virus between one and five days after experimental challenge and degree of transmission to
in contact animals. The studies showed that complete protection and prevention of transmission is afforded after
five days, with some protection evident as soon as three days post vaccination. The strain of virus did not affect
the degree of protection afforded.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
VLA collaborated with the Changchun Veterinary Research Institute in an analysis of strains of CSF virus from P.
R. China.
7.
Provision of consultant expertise to OIE or to OIE Members
The Reference Expert provided lectures on classical swine fever at a regional meeting of veterinarians in
Krasnodar, Russian Federation and in Davao City, The Philippines. He also provided scientific lectures at
scientific meetings in Harbin and Changchun, P. R. China.
8.
Provision of scientific and technical training to personnel from other OIE Members
A visiting scientist was given training in CSF diagnosis and participated in the research carried out at VLA, during
a two month visit funded by the OIE Twinning scheme.
9.
Provision of diagnostic testing facilities to other OIE Members
None
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
None
11. Participation in international scientific collaborative studies
VLA participated in a collaborative study with the Changchun Veterinary Research Institute, P. R. China, to
characterise strains of CSF in that country.
12. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)

Presentations at international conferences and meetings
Everett, H. G., S., Clifford, D., Johns, H., Haines, F., Sosan, O., Salguero F. J , Drew, T., and Crooke H. (2009b).
Efficacy of C-strain vaccine in domestic pigs challenged with genotype 2.1 and 3.3 classical swine fever virus
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
Classical swine fever
shortly after vaccination. In 8th international Congress of Veterinary Virology. Budapest, Hungary, 23-26th
August 2009.
Everett, H. G., S., Clifford, D., Johns, H., Haines, F., Sosan, O., Salguero F. J , Drew, T., and Crooke H. (2009).
Efficacy of C-strain vaccine in domestic pigs challenged with genotype 2.1 and 3.3 CSFV shortly after
vaccination. In Annual meeting of National Swine Fever Laboratories, . Valdeolmos, (Madrid) Spain, June 15th16th 2009
Everett, H., Graham, S., Haines, F., Johns, H., Salguero F. J ., Nunez , A., Steinbach, F., Drew, T., and Crooke H.
(2009). Assessment of protection following vaccination of pigs with a live attenuated vaccine against classical
swine fever virus. In American Society for Virology, 28th Annual Meeting. University of British Columbia,
Vancouver, Canada, 11th 15th July 2009
Drew, T. W., Graham, S. and Crooke, H. (2009). New technologies to control classical swine fever (CSF)In The
5th International Veterinary Vaccines and Diagnostics Conference Madison, WI USA, July 19-23, 2009.
Graham, P. S., Haines, F., Johns,H., Lamp, B., Ruemenapf, T., Everett,H., and Crooke,H. (2009). Investigation of
vaccine-induced T cell responses in mediating rapid protection against classical swine fever In 3rd European
Veterinary Immunology Workshop. Berlin, 10-13th Sept 2009.
Everett, H., Sosan, O., and Crooke H. (2009). A differential PCR approach to monitor Classical Swine Fever Virus
challenge strains during experimental infection of C-strain vaccinated pigs. In 3rd Epizone Annual Meeting
Antalya Turkey, 12-15th July
Graham, S., Everett, H., Johns, H., Haines, F., La Rocca, A. and Crooke, H. (2009) Characterization of virusspecific T cell cytokine responses following vaccination and challenge with classical swine fever viruses. Poster
presentation at the British Society for Immunology Annual Congress, 17-21st November 2008, Glasgow

Scientific publications in peer-reviewed journals
Everett, H., Salguero, F.J., Graham, S.P., Haines, F., Johns, H., Clifford, D., Nunez, A., La Rocca, S.A.,
Parchariyanon, S., Steinbach, F., Drew, T., Crooke, H., 2009, Characterisation of experimental infections of
domestic pigs with genotype 2.1 and 3.3 isolates of classical swine fever virus. Vet Microbiol. Online
Graham, S.P., Everett, H.E., Johns, H.L., Haines, F.J., Rocca, S.A., Khatri, M., Wright, I.K., Drew, T., Crooke,
H.R., 2009, Characterisation of virus-specific peripheral blood cell cytokine responses following vaccination or
infection with classical swine fever viruses. Vet Microbiol. Online
Johns, H.L., Bensaude, E., La Rocca, S.A., Seago, J., Charleston, B., Steinbach, F., Drew, T.W., Crooke, H.,
Everett, H., 2009, Classical Swine Fever Virus infection protects aortic endothelial cells from pIpC-mediated
apoptosis. J Gen Virol. Online
Zhu, Y., Shi, Z., Drew, T.W., Wang, Q., Qiu, H., Guo, H., Tu, C., 2009, Antigenic differentiation of classical
swine fever viruses in China by monoclonal antibodies. Virus Res 142, 169-174.

Other communications
Drew T. W. (2009) Classical swine fever. Seminar, Pinoy Pork Challenge, Davao City, Philippines, Feb 2009
Drew, T. W. (2009) Classical swine fever. Seminar, Krasnodar Agricultural University, Krasnodarskiy Kray,
Russia, Oct 2009
Graham, S. (2009) The role of vaccine-induced T cell responses in mediating rapid protection against classical
swine fever. Institute for Animal Health Seminar, 27th May 2009, IAH-Compton
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009
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Classical swine fever
13. Inscription of diagnostic kits on the OIE Register
i)
Did you participate in expert panels for the validation of candidate kits for inscription on the
OIE Register? If yes, for which kits?
None
ii)
Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for
which kits?
None
Acknowledgements:
The role of VLA Weybridge as an OIE Reference Laboratory for CSF is funded by the UK Department for
Environment, Food and Rural Affairs (Defra) under contract SV3000. Vaccine studies were funded under Defra
contract SE0778. This report was prepared by Trevor Drew and Helen Crooke.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2009