赣 南 医 学 院 教 案 教研室:Department of Biochemistry and Molecular Biology 课程名称 授课内容 Biochemistry 授课专业 Chapter14 Gene Recombination and Gene Project 教师姓名: Xu Chunjuan Clinical medicine major 授课学时 4 【Master】 Conceptions of genetic engineering, gene diagnosis, and gene therapy. 教学目的 【Understand】 Common tools used in genetic engineering, polymerase chain reaction. 【Know】 DNA Sequencing. 教学重点 Conceptions of genetic engineering, gene diagnosis, and gene therapy. 教学难点 Genetic engineering 1. Theaching aid: computer, projector, mike, chalk, ferule, multimedia courseware 教具和媒 体使用 2. Theaching means: utilize modernization teaching means and methods, make good courseware, combine pictures with words well; integrate languge expression, apporiate writing and teaching with multimedia. 1. Introduction of Dolly. 2. Focused, decentralized and difficult, and easy-to seize key; 3. Language, illustrated the integration of multimedia courseware, and appropriate progress on related research; 4. Combination of antibiotics and other biological active substance of 教学方法 the role of an example, the basic theory and clinical practice to closely integrate; 5. In some key or key, can be properly writing on the blackboard, a focused, and guide students to more easily grasp the ideas; 6. Classrooms and more questions, and student exchange, mobilize their enthusiasm, but also a first suspect, in the follow-up teaching guide students to find answers to simple terms, stripping layers. Teaching Process is divided into 3 parts : guide,main content and summary. 教学过程 Guide: Introduction of Dolly. Main content: 1.Genetic Engineering (1) Common tools used in genetic engineering (2) Processes of genetic engineering 2. Molecular Hybridization (1) Nucleic acid probes (2) Types of molecular hybridization 3.Polymerase Chain Reaction (1) Principles of polymerase chain reaction (2) Procedures of polymerase chain reaction (3)Applications of polymerase chain reaction 4. DNA Sequencing (1)Chemical cleavage sequencing (2) Dideoxy chain terminator sequencing 5.Transgenesis, Animal Cloning and Gene Knockout Technologies (1)Transgenesis (2)Animal cloning technology (3)Gene knockout technology (4)Biomedical applications 6.Gene Diagnosis (1)Common technologies used in gene diagnosis (2) Applications of gene diagnosis 7.Gene Therapy (1) Conceptions of gene therapy (2) The procedures of gene therapy (3) Applications of gene therapy summary 讲授新进展 内容 参考书籍 思考题或/及 作业 课后总结 Gene therapy and the technology of real time PCR. ⑴ Berg J M,Tymoczko J L,Stryer L. 2002. Biochemistry. 5th Edition. New York:W.H. Freeman Publishers. ⑵ Devlin T M.. 2002. Textbook of Biochemisrty with Clinical Correlations. New York: Wiley-Liss. ⑶ Robert K. Murray, Daryl K. Granner, Peter A. Mayes,et al. 2003. Harper’s Biochemistry. Stamford:McGraw-Hill. Review the content to be mastered. Main content of this chapter is summarized into six terms,which are separating, cutting, ligating,transfecting,screening and expression.