赣 南 医 学 院 教 案
教研室：Department of Biochemistry and Molecular Biology
课程名称
授课内容
Biochemistry
授课专业
Chapter14 Gene Recombination and
Gene Project
教师姓名： Xu Chunjuan
Clinical medicine major
授课学时
4
【Master】
Conceptions of genetic engineering, gene diagnosis, and gene therapy.
教学目的
【Understand】
Common tools used in genetic engineering, polymerase chain reaction.
【Know】
DNA Sequencing.
教学重点
Conceptions of genetic engineering, gene diagnosis, and gene therapy.
教学难点
Genetic engineering
1. Theaching aid: computer, projector, mike, chalk, ferule, multimedia
courseware
教具和媒
体使用
2. Theaching means: utilize modernization teaching means and
methods, make good courseware, combine pictures with words well;
integrate languge expression, apporiate writing and teaching with
multimedia.
1. Introduction of Dolly.
2. Focused, decentralized and difficult, and easy-to seize key;
3. Language, illustrated the integration of multimedia courseware, and
appropriate progress on related research;
4. Combination of antibiotics and other biological active substance of
教学方法
the role of an example, the basic theory and clinical practice to closely
integrate;
5. In some key or key, can be properly writing on the blackboard, a
focused, and guide students to more easily grasp the ideas;
6. Classrooms and more questions, and student exchange, mobilize
their enthusiasm, but also a first suspect, in the follow-up teaching guide
students to find answers to simple terms, stripping layers.
Teaching Process is divided into 3 parts : guide,main content and summary.
教学过程

Guide: Introduction of Dolly.

Main content:
1.Genetic Engineering
(1) Common tools used in genetic engineering
(2) Processes of genetic engineering
2. Molecular Hybridization
(1) Nucleic acid probes
(2) Types of molecular hybridization
3.Polymerase Chain Reaction
(1) Principles of polymerase chain reaction
(2) Procedures of polymerase chain reaction
(3)Applications of polymerase chain reaction
4. DNA Sequencing
(1)Chemical cleavage sequencing
(2) Dideoxy chain terminator sequencing
5.Transgenesis, Animal Cloning and Gene Knockout Technologies
(1)Transgenesis
(2)Animal cloning technology
(3)Gene knockout technology
(4)Biomedical applications
6.Gene Diagnosis
(1)Common technologies used in gene diagnosis
(2) Applications of gene diagnosis
7.Gene Therapy
(1) Conceptions of gene therapy
(2) The procedures of gene therapy
(3) Applications of gene therapy
summary
讲授新进展
内容
参考书籍
思考题或/及
作业
课后总结
Gene therapy and the technology of real time PCR.
⑴ Berg J M，Tymoczko J L，Stryer L. 2002. Biochemistry. 5th
Edition. New York:W.H. Freeman Publishers.
⑵ Devlin T M.. 2002. Textbook of Biochemisrty with Clinical
Correlations. New York: Wiley-Liss.
⑶ Robert K. Murray, Daryl K. Granner, Peter A. Mayes，et al. 2003.
Harper’s Biochemistry. Stamford：McGraw-Hill.
Review the content to be mastered.
Main content of this chapter is summarized into six terms,which are
separating, cutting, ligating,transfecting,screening and expression.