Intro to Microscopes Worksheet
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Name _____________________________
Date ___________________ Period _____
Go to: http://learn.genetics.utah.edu/content/begin/cells/scale/.
1.
Object
Predicted order:
Biggest to smallest
Actual Size
glucose
ribosome
grain of salt
Carbon atom
E. coli bacterium
influenza virus
skin cell
hemoglobin
amoeba
2. Write the correct name for each metric unit abbreviation.
mm ______________________
µm ______________________
nm ______________________
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pm ______________________
Answer the following questions from the “Microscopy” PowerPoint presentation.
3. a) What is another name for an “optical” microscope? _____________ microscope
b) What is the approximate range of sizes for items that would be best viewed with an optical
microscope?
c) What is the range of sizes for most plant and animal cells?
d) True or False? A bacterium can be viewed with an optical microscope.
e) True or False? A virus can be viewed with an optical microscope.
f) What is the approximate range of sizes for items that would be best viewed with an electron
microscope?
4. Below each picture, write the type of microscope that
would have been used to create the image.
5. Label the parts of the light microscope below using pages 21 and/or 1088 of your textbook.
High Power Objective
Low Power Objective
Stage clips
Condenser/Diaphragm
6. Complete the table by describing the function of each part:
Part
Function
Objective lenses
Eyepiece
Diaphragm (condenser)
Coarse adjustment knob
Fine adjustment knob
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Refer to Section 1-4 of the textbook.
For questions #7-8, circle the one term that does not belong. Explain why it does not belong.
7. light source, scanning power, objective lens, eyepiece
8. compound light, transmission electron, light electron, scanning electron
9. The ability of a microscope to show details clearly is called ______________________________.
10. The ability of a microscope to increase an object’s apparent sized is called __________________.
11. To view a specimen with a light microscope, the specimen must be sliced __________ or be very
small.
12. Where is the “ocular lens” located on a light microscope?
13. Arrange the following parts in the order that matches the light path through a light microscope:
specimen, ocular lens, objective lens, body tube, light source
______________, ______________, ______________, ______________, ______________
14. How do you calculate the power of magnification of a microscope?
15. For each pair of lenses, calculate the total magnification for the microscope.
Eyepiece Lens
Objective Lens
Total Magnification
10x
4x
20x
10x
40x
16. What is the maximum magnification for each type of microscope below?
a) light microscope (LM)
b) TEM
c) SEM
17. What problem occurs when a specimen is attempted to be viewed beyond 2000x magnification
with a light microscope?
18. Scientists use electron microscopes to view specimens that are even smaller than cells, such as
________ _________ or ______________.
19. What is an important limitation of both the TEM and SEM?
20. Time for some critical thinking! A group of scientists want to determine whether the bacteria they
are studying have viruses inside them. Which type of microscope should they use? Explain your
answer.
Participate in the microscope review SMART board game provided by your teacher. Record
your answers below.
Question
1
2
3
4
Parts
Rules
How it Works