Level 1 Enzymes & Cellular Respiration Review Guide Organize your notes in the following order: 1. Study guide packet with homework assignments 2. Notes packet with the following: Metabolic pathways & enzymes (substrate, energy of activation, active site, naming, induced fit model, factors affecting enzyme function) – p. 1 – 2 (front) The ATP cycle (back p. 2_ Overview of cellular respiration (aerobic, redox, NAD+ & FAD, 4 phases, equation) – p. 3) Glycolysis (back 3) Inside the mitochondria (cristae, matrix, 4 phases cell respiration & locations, transition reaction) – p. 4 Citric acid cycle – back 4 Electron transport system – p. 5 Energy yield from glucose metabolism – p.6 Fermentation – back 6 - 7 3. Enzyme activity 4. Glycolysis diagram 5. Citric acid cycle diagram 6. Cellular respiration diagram 7. Fermentation diagram Test questions will be based on the following main ideas: Be able to explain the importance of enzymes to metabolic pathways and how they affect the energy of activation for a reaction Be able to explain how enzymes are named and why they are named in such a manner. Be able to explain what happens when an enzyme binds with its substrate, and the model used to describe this interaction. Be able to explain the following factors and their effects on enzyme activity: substrate concentration, temperature, pH, enzyme concentration, feedback inhibition (competitive and noncompetitive) If given a set of data, or description of factors, be able to use enzyme functioning to explain the given data. Be able to explain what is meant by the terms “oxidation” and “reduction,” and be able to identify the enzymes that are oxidized and reduced during cell respiration. Know the overall equation for cellular respiration, the phases involved, and when each of the products are made during the process. Know the steps a cell would undergo to make ATP during both aerobic and anaerobic conditions. Be familiar with organisms who would use each set of processes, and when each type of respiration might occur. Know where all of the following occur: glycolysis, transition reaction, citric acid cycle, electron transport system, fermentation. Be able to identify the inputs and outputs of each of the following processes: glycolysis, transition reaction, citric acid cycle, electron transport system, fermentation. Be able to explain how the electron transport system works. Be prepared to explain the significance of NADH and FADH2, the role of the protein complexes, mobile carriers, and ATP synthase complex, involvement of electrons and hydrogen ions, and the parts of the mitochondria. Be able to calculate (in both a prokaryote and a eukaryote) the total amount of ATP produced through aerobic cellular respiration and anaerobic cellular respiration. Be able to explain why the difference exists between aerobic prokaryotes and eukaryotes. Be able to label a diagram showing locations of all processes, and products of each.