Protein View
Match to: gi|28971736 Score: 90 Expect: 0.0061
chitinase III [Vitis vinifera]
Nominal mass (Mr): 32212; Calculated pI value: 8.84
NCBI BLAST search of gi|28971736 against nr
Unformatted sequence string for pasting into other applications
Taxonomy: Vitis vinifera
Fixed modifications: Carbamidomethyl (C)
Variable modifications: Oxidation (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 22
Number of mass values matched: 7
Sequence Coverage: 26%
Matched peptides shown in Bold Red
1
51
101
151
201
251
MARTPQSTPL
SYVNIAFLNK
LSIGGGVGSY
IELGSTLHWD
DYVWVQFYNN
SGFIPANVLT
LISLSVLALL
FGNGQTPEIN
SLSSSNDAQN
DLARALSGFS
PQCQYSSGNT
SQILPVIKRS
Residue Number
QTSYAGGIAI
LAGHCNPASN
VANYLWNNFL
KRGRKVYLTA
NNLLNSWNRW
PKYGGVMLWS
Increasing Mass
Start - End
Observed
Mr(expt)
50 - 60
1331.7340 1330.7268
K.YSYVNIAFLNK.F
61 - 90
3116.7301 3115.7228
K.FGNGQTPEINLAGHCNPASNGCTSVSTGIR.N
175 - 189
1734.9294 1733.9221
R.KVYLTAAPQCPFPDK.F
176 - 189
1606.8468 1605.8396
K.VYLTAAPQCPFPDK.F
273 - 281
1040.5098 1039.5025
K.YGGVMLWSK.Y
273 - 281
1056.5240 1055.5168
K.YGGVMLWSK.Y Oxidation (M)
282 - 294
1512.7075 1511.7002
K.YYDDQSGYSSSIK.S
YWGQNGNEGT
GCTSVSTGIR
GGQSSSRPLG
APQCPFPDKF
TSSINSQIFM
KYYDDQSGYS
LTQTCNTGKY
NCQNRGIKVM
DAVLDGIDFD
LGTALNTGLF
GLPASSAAAG
SSIKSSV
Decreasing Mass
Mr(calc)
1330.6921
ppm
26
Miss Sequence
0
3115.4200
97
0
1733.8811
24
1
1605.7861
33
0
1039.5161
-13
0
1055.5110
5
0
1511.6416
39
0
No match to: 855.0983, 870.5729, 871.0637, 881.3040, 962.4686, 1393.6446,
1576.7964, 1668.7975, 2235.2973, 2273.1611, 2540.3922, 2716.4525, 2807.5394,
2953.6763, 3310.9216
LOCUS
2006
DEFINITION
ACCESSION
VERSION
DBSOURCE
KEYWORDS
SOURCE
ORGANISM
BAC65326
297 aa
linear
PLN 21-JAN-
chitinase III [Vitis vinifera].
BAC65326
BAC65326.1 GI:28971736
accession AB105374.1
.
Vitis vinifera
Vitis vinifera
Eukaryota; Viridiplantae; Streptophyta; Embryophyta;
Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; core
eudicotyledons;
Vitales; Vitaceae; Vitis.
REFERENCE
1
AUTHORS
Ano,A., Takayanagi,T., Uchibori,T., Okuda,T. and Yokotsuka,K.
TITLE
Characterization of a class III chitinase from Vitis vinifera cv.
Koshu
JOURNAL
J. Biosci. Bioeng. 95 (6), 645-647 (2003)
PUBMED
16233474
REFERENCE
2 (residues 1 to 297)
AUTHORS
Takayanagi,T.
TITLE
Direct Submission
JOURNAL
Submitted (10-MAR-2003) Tsutomu Takayanagi, University of
Yamanashi, Institute of Enology and Viticulture; 1-13-1 Kitashin,
Kofu, Yamanashi 400-0005, Japan
(E-mail:takayana@mail.yamanashi.ac.jp, Tel:81-55-220-8607,
Fax:81-55-220-8768)
FEATURES
Location/Qualifiers
source
1..297
/organism="Vitis vinifera"
/cultivar="Koshu"
/db_xref="taxon:29760"
Protein
1..297
/product="chitinase III"
Region
26..>243
/region_name="Glyco_hydro_18"
/note="Glycosyl hydrolases family 18; cl01310"
/db_xref="CDD:91756"
CDS
1..297
/coded_by="AB105374.1:54..947"
Mascot: http://www.matrixscience.com/
Protein View
Match to: gi|147791187 Score: 90 Expect: 0.0061
hypothetical protein [Vitis vinifera]
Nominal mass (Mr): 32204; Calculated pI value: 8.84
NCBI BLAST search of gi|147791187 against nr
Unformatted sequence string for pasting into other applications
Taxonomy: Vitis vinifera
Fixed modifications: Carbamidomethyl (C)
Variable modifications: Oxidation (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 22
Number of mass values matched: 7
Sequence Coverage: 26%
Matched peptides shown in Bold Red
1
51
101
151
201
251
MAXTPQSTPL
SYVNIAFLNK
LSIGGGXGSY
IELGSTLHWD
DYVWVQFYNN
SGFIPANVLT
LISLSVLALL
FGNGQTPEIN
SLSSSNDAQN
DLARALSGFS
XPCQYSSGNT
SQILPVIKRS
Residue Number
QTSYAGGIAI
LAGHCNPASN
VANYLWNNFL
KRGRKVYLTA
NNLLNSWNRW
XKYGGVMLWS
Increasing Mass
Start - End
Observed
Mr(expt)
50 - 60
1331.7340 1330.7268
K.YSYVNIAFLNK.F
61 - 90
3116.7301 3115.7228
K.FGNGQTPEINLAGHCNPASNGCTSVSTGIR.N
175 - 189
1734.9294 1733.9221
R.KVYLTAAPQCPFPDK.F
176 - 189
1606.8468 1605.8396
K.VYLTAAPQCPFPDK.F
273 - 281
1040.5098 1039.5025
K.YGGVMLWSK.Y
273 - 281
1056.5240 1055.5168
K.YGGVMLWSK.Y Oxidation (M)
282 - 294
1512.7075 1511.7002
K.YYDDQSGYSSSIK.S
YWGQNGNEGT
GCTSVSTGIR
GGQSSSRPLG
APQCPFPDKF
TSSINSRIFM
KYYDDQSGYS
LTQTCNTGKY
NCQNRGIKVM
DAVLDGIDFD
LGTALNTGLF
GLPASSAAAG
SSIKSSV
Decreasing Mass
Mr(calc)
1330.6921
ppm
26
Miss Sequence
0
3115.4200
97
0
1733.8811
24
1
1605.7861
33
0
1039.5161
-13
0
1055.5110
5
0
1511.6416
39
0
No match to: 855.0983, 870.5729, 871.0637, 881.3040, 962.4686, 1393.6446,
1576.7964, 1668.7975, 2235.2973, 2273.1611, 2540.3922, 2716.4525, 2807.5394,
2953.6763, 3310.9216
LOCUS
2008
DEFINITION
ACCESSION
VERSION
DBSOURCE
KEYWORDS
SOURCE
CAN61462
297 aa
hypothetical protein [Vitis vinifera].
CAN61462
CAN61462.1 GI:147791187
embl accession AM462355.2
.
Vitis vinifera
linear
PLN 05-FEB-
ORGANISM
Vitis vinifera
Eukaryota; Viridiplantae; Streptophyta; Embryophyta;
Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; core
eudicotyledons;
Vitales; Vitaceae; Vitis.
REFERENCE
1
AUTHORS
Velasco,R., Zharkikh,A., Troggio,M., Cartwright,D.A., Cestaro,A.,
Pruss,D., Pindo,M., Fitzgerald,L.M., Vezzulli,S., Reid,J.,
Malacarne,G., Iliev,D., Coppola,G., Wardell,B., Micheletti,D.,
Macalma,T., Facci,M., Mitchell,J.T., Perazzolli,M., Eldredge,G.,
Gatto,P., Oyzerski,R., Moretto,M., Gutin,N., Stefanini,M.,
Chen,Y.,
Segala,C., Davenport,C., Dematte,L., Mraz,A., Battilana,J.,
Stormo,K., Costa,F., Tao,Q., Si-Ammour,A., Harkins,T., Lackey,A.,
Perbost,C., Taillon,B., Stella,A., Solovyev,V., Fawcett,J.A.,
Sterck,L., Vandepoele,K., Grando,S.M., Toppo,S., Moser,C.,
Lanchbury,J., Bogden,R., Skolnick,M., Sgaramella,V.,
Bhatnagar,S.K., Fontana,P., Gutin,A., Van de Peer,Y., Salamini,F.
and Viola,R.
TITLE
A high quality draft consensus sequence of the genome of a
heterozygous grapevine variety
JOURNAL
(er) PLoS ONE 2 (12), E1326 (2007)
PUBMED
18094749
REFERENCE
2
AUTHORS
Velasco,R.
TITLE
Direct Submission
JOURNAL
Submitted (19-DEC-2006) Velasco R., Genetics and Molecular
Biology,
istituto agrario 'San Michele all'Adige', Via Edmondo Mach, 1.
San
Michele all'Adige (Tn), 38010, ITALY
REMARK
revised by [3]
REFERENCE
3 (residues 1 to 297)
AUTHORS
Velasco,R.
TITLE
Direct Submission
JOURNAL
Submitted (04-MAY-2007) IASMA, Genetics and Molecular Biology
Department, Via Edmondo Mach, 1 38010 S. Michele all'Adige (TN) ITALY
COMMENT
NOTE: This is a 'working draft' sequence. The true order of the
contigs and the gap size among them are not known. The actual
genome coverage is 11X, 7X sequenced by Sanger method and 4X by
pyrosequencing. Sequencing effort has been performed by IASMA,
Myriad Genetics and 454 Life Science. The gene prediction has
been
performed by FGENESH program in collaboration with Softberry,
while
the identified genes has been annotated by similarity search and
their function has been described using Gene Ontology terms
(www.geneontology.org).
FEATURES
Location/Qualifiers
source
1..297
/organism="Vitis vinifera"
/cultivar="Pinot Noir"
/db_xref="taxon:29760"
/chromosome="16"
/clone="ENTAV 115"
Protein
1..297
/product="hypothetical protein"
26..>243
/region_name="Glyco_hydro_18"
/note="Glycosyl hydrolases family 18; cl01310"
/db_xref="CDD:91756"
1..297
/locus_tag="VITISV_038584"
/coded_by="AM462355.2:10687..11580"
/inference="similar to DNA sequence:EMBL:AB080760"
/note="GO:0004553 GO:0016798 GO:0016787 GO:0003824
GO:0043169 GO:0005975 GO:0000272 GO:0006032
highly similar to EMBL:AB080760"
/db_xref="GOA:A5BK69"
/db_xref="InterPro:IPR001223"
/db_xref="InterPro:IPR001579"
/db_xref="InterPro:IPR013781"
/db_xref="UniProtKB/TrEMBL:A5BK69"
Region
CDS
Mascot: http://www.matrixscience.com/
Protein View
Match to: gi|157360148 Score: 90 Expect: 0.0061
unnamed protein product [Vitis vinifera]
Nominal mass (Mr): 32157; Calculated pI value: 8.98
NCBI BLAST search of gi|157360148 against nr
Unformatted sequence string for pasting into other applications
Taxonomy: Vitis vinifera
Fixed modifications: Carbamidomethyl (C)
Variable modifications: Oxidation (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 22
Number of mass values matched: 7
Sequence Coverage: 26%
Matched peptides shown in Bold Red
1
51
101
151
201
251
MARTPQSTPL
SYVNIAFLNK
LSIGGGVGSY
IELGSTLHWD
DYVWVQFYNN
SGFIPANVLT
LISLSVLALL
FGNGQTPEIN
SLSSSNDAQN
DLARALSGFS
APCQYSSGNT
SQILPVIKRS
Residue Number
QTSYAGGIAI
LAGHCNPASN
VANYLWNNFL
KRGRKVYLTA
NNLLNSWNRW
AKYGGVMLWS
Increasing Mass
YWGQNGNEGT
GCTSVSTGIR
GGQSSSRPLG
APQCPFPDKF
TSSINSRIFM
KYYDDQSGYS
LTQTCNTGKY
NCQNRGIKVM
DAVLDGIDFD
LGTALNTGLF
GLPASSAAAG
SSIKSSV
Decreasing Mass
Start - End
Observed
Mr(expt)
50 - 60
1331.7340 1330.7268
K.YSYVNIAFLNK.F
61 - 90
3116.7301 3115.7228
K.FGNGQTPEINLAGHCNPASNGCTSVSTGIR.N
175 - 189
1734.9294 1733.9221
R.KVYLTAAPQCPFPDK.F
176 - 189
1606.8468 1605.8396
K.VYLTAAPQCPFPDK.F
273 - 281
1040.5098 1039.5025
K.YGGVMLWSK.Y
273 - 281
1056.5240 1055.5168
K.YGGVMLWSK.Y Oxidation (M)
282 - 294
1512.7075 1511.7002
K.YYDDQSGYSSSIK.S
Mr(calc)
1330.6921
ppm
26
Miss Sequence
0
3115.4200
97
0
1733.8811
24
1
1605.7861
33
0
1039.5161
-13
0
1055.5110
5
0
1511.6416
39
0
No match to: 855.0983, 870.5729, 871.0637, 881.3040, 962.4686, 1393.6446,
1576.7964, 1668.7975, 2235.2973, 2273.1611, 2540.3922, 2716.4525, 2807.5394,
2953.6763, 3310.9216
LOCUS
2007
DEFINITION
ACCESSION
VERSION
DBSOURCE
KEYWORDS
SOURCE
ORGANISM
CAO69337
297 aa
linear
PLN 08-OCT-
unnamed protein product [Vitis vinifera].
CAO69337
CAO69337.1 GI:157360148
embl accession CU459388.1
.
Vitis vinifera
Vitis vinifera
Eukaryota; Viridiplantae; Streptophyta; Embryophyta;
Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; core
eudicotyledons;
Vitales; Vitaceae; Vitis.
REFERENCE
1
AUTHORS
Jaillon,O., Aury,J.M., Noel,B., Policriti,A., Clepet,C.,
Casagrande,A., Choisne,N., Aubourg,S., Vitulo,N., Jubin,C.,
Vezzi,A., Legeai,F., Hugueney,P., Dasilva,C., Horner,D., Mica,E.,
Jublot,D., Poulain,J., Bruyere,C., Billault,A., Segurens,B.,
Gouyvenoux,M., Ugarte,E., Cattonaro,F., Anthouard,V., Vico,V.,
Del
Fabbro,C., Alaux,M., Di Gaspero,G., Dumas,V., Felice,N.,
Paillard,S., Juman,I., Moroldo,M., Scalabrin,S., Canaguier,A., Le
Clainche,I., Malacrida,G., Durand,E., Pesole,G., Laucou,V.,
Chatelet,P., Merdinoglu,D., Delledonne,M., Pezzotti,M.,
Lecharny,A., Scarpelli,C., Artiguenave,F., Pe,M.E., Valle,G.,
Morgante,M., Caboche,M., Adam-Blondon,A.F., Weissenbach,J.,
Quetier,F. and Wincker,P.
CONSRTM
French-Italian Public Consortium for Grapevine Genome
Characterization
TITLE
The grapevine genome sequence suggests ancestral hexaploidization
in major angiosperm phyla
JOURNAL
PUBMED
REMARK
REFERENCE
AUTHORS
TITLE
JOURNAL
de
Nature 449 (7161), 463-467 (2007)
17721507
DOI:10.1038/nature06148
2 (residues 1 to 297)
Quetier,F.
Direct Submission
Submitted (29-MAR-2007) Quetier F., Genoscope - Centre National
Sequencage, CP 5706 EVRY Cedex, F-91057, FRANCE
Location/Qualifiers
1..297
/organism="Vitis vinifera"
/cultivar="PN40024"
/db_xref="taxon:29760"
/chromosome="undetermined"
/note="Genoscope sequence ID : scaffold_171"
Protein
1..297
/name="unnamed protein product"
Region
26..>243
/region_name="Glyco_hydro_18"
/note="Glycosyl hydrolases family 18; cl01310"
/db_xref="CDD:91756"
CDS
1..297
/locus_tag="GSVIVT00006464001"
/coded_by="complement(CU459388.1:184699..185592)"
FEATURES
source
Mascot: http://www.matrixscience.com/
Protein View
Match to: gi|1705812 Score: 90 Expect: 0.0067
Acidic endochitinase precursor
Nominal mass (Mr): 32673; Calculated pI value: 8.69
NCBI BLAST search of gi|1705812 against nr
Unformatted sequence string for pasting into other applications
Taxonomy: Vitis vinifera
Links to retrieve other entries containing this sequence from NCBI Entrez:
gi|1150686 from Vitis vinifera
Fixed modifications: Carbamidomethyl (C)
Variable modifications: Oxidation (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 22
Number of mass values matched: 7
Sequence Coverage: 25%
Matched peptides shown in Bold Red
1 MARTPQSTPL LISLSVLALL QTSYAGGIAI YWGQNGNEGT LTQTCNTGKY
51 SYVNIAFLNK FGNGQTPEIN LAGHCNPASN GCTSVSTGIR NCQNRGIKVM
101 LSIGGGAGSY SLSSSNDAQN VANYLWNNFL GGQSSSRPLG DAVLDGIDFD
151
201
251
301
IELGSTLHWD DLARALSRIE FQQERGRKVY LTAAPQCPFP DKVPGTALNT
GLFDYVWVQF YNNPPCQYSS GNTNNLLNSW NRWTSSINST GSFMGLPASS
AAAGRGFIPA NVLTSQILPV IKRSPKYGGV MLWSKYYDDQ SGYSSSIKSS
V
Show predicted peptides also
Sort Peptides By
Residue Number
Increasing Mass
Start - End
Observed
Mr(expt)
50 - 60
1331.7340 1330.7268
K.YSYVNIAFLNK.F
61 - 90
3116.7301 3115.7228
K.FGNGQTPEINLAGHCNPASNGCTSVSTGIR.N
178 - 192
1734.9294 1733.9221
R.KVYLTAAPQCPFPDK.V
179 - 192
1606.8468 1605.8396
K.VYLTAAPQCPFPDK.V
277 - 285
1040.5098 1039.5025
K.YGGVMLWSK.Y
277 - 285
1056.5240 1055.5168
K.YGGVMLWSK.Y Oxidation (M)
286 - 298
1512.7075 1511.7002
K.YYDDQSGYSSSIK.S
Mr(calc)
1330.6921
Decreasing Mass
ppm
26
Miss Sequence
0
3115.4200
97
0
1733.8811
24
1
1605.7861
33
0
1039.5161
-13
0
1055.5110
5
0
1511.6416
39
0
No match to: 855.0983, 870.5729, 871.0637, 881.3040, 962.4686, 1393.6446,
1576.7964, 1668.7975, 2235.2973, 2273.1611, 2540.3922, 2716.4525, 2807.5394,
2953.6763, 3310.9216
LOCUS
2008
DEFINITION
ACCESSION
VERSION
DBSOURCE
P51614
301 aa
linear
Acidic endochitinase precursor.
P51614
P51614.1 GI:1705812
swissprot: locus CHIT3_VITVI, accession P51614;
class: standard.
created: Oct 1, 1996.
sequence updated: Oct 1, 1996.
annotation updated: May 20, 2008.
xrefs: Z68123.1, CAA92207.1
PLN 20-MAY-
xrefs (non-sequence databases): HSSP:P23472, SMR:P51614,
GO:0016798, GO:0006032, InterPro:IPR001223, InterPro:IPR001579,
InterPro:IPR013781, Gene3D:G3DSA:3.20.20.80, Pfam:PF00704,
PROSITE:PS01095
KEYWORDS
Carbohydrate metabolism; Chitin degradation; Glycosidase;
Hydrolase; Polysaccharide degradation; Signal.
SOURCE
Vitis vinifera
ORGANISM Vitis vinifera
Eukaryota; Viridiplantae; Streptophyta; Embryophyta;
Tracheophyta;
Spermatophyta; Magnoliophyta; eudicotyledons; core
eudicotyledons;
Vitales; Vitaceae; Vitis.
REFERENCE
1 (residues 1 to 301)
AUTHORS
Busam,G., Kassemeyer,H.H. and Matern,U.
TITLE
Differential expression of chitinases in Vitis vinifera L.
responding to systemic acquired resistance activators or fungal
challenge
JOURNAL
Plant Physiol. 115 (3), 1029-1038 (1997)
PUBMED
9390436
REMARK
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Pinot
COMMENT
[FUNCTION] Defense against chitin containing fungal pathogens.
[CATALYTIC ACTIVITY] Random hydrolysis of
N-acetyl-beta-D-glucosaminide 1,4-beta-linkages in chitin and
chitodextrins.
[SIMILARITY] Belongs to the glycosyl hydrolase 18 family.
Chitinase
class II subfamily.
FEATURES
Location/Qualifiers
source
1..301
/organism="Vitis vinifera"
/db_xref="taxon:29760"
gene
1..301
/gene="CHIT3"
Protein
1..301
/gene="CHIT3"
/product="Acidic endochitinase precursor"
/EC_number="3.2.1.14"
Region
1..25
/gene="CHIT3"
/region_name="Signal"
/inference="non-experimental evidence, no additional
details recorded"
/note="Potential."
Region
26..301
/gene="CHIT3"
/region_name="Mature chain"
/experiment="experimental evidence, no additional
details
recorded"
/note="Acidic endochitinase. /FTId=PRO_0000011921."
Region
26..285
/gene="CHIT3"
/region_name="Glyco_hydro_18"
/note="Glycosyl hydrolases family 18; cl01310"
/db_xref="CDD:91756"
Bond
Bond
Site
Bond
bond(45,92)
/gene="CHIT3"
/bond_type="disulfide"
/inference="non-experimental evidence,
details recorded"
/note="By similarity."
bond(75,82)
/gene="CHIT3"
/bond_type="disulfide"
/inference="non-experimental evidence,
details recorded"
/note="By similarity."
152
/gene="CHIT3"
/site_type="active"
/inference="non-experimental evidence,
details recorded"
/note="Proton donor (By similarity)."
bond(187,216)
/gene="CHIT3"
/bond_type="disulfide"
/inference="non-experimental evidence,
details recorded"
/note="By similarity."
Mascot: http://www.matrixscience.com/
no additional
no additional
no additional
no additional