®
®
March 2015
Rev. No: 00
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
Gel Documentation Form and Worksheet
DQB1*06
(101.212-24/04 – 24u/04u)
Lot No: 93X
Expiry Date: 2017-September-01
Name:__________________
Test Date:______________
Review Date:_______________
Sample ID:______________
Tested By:______________
Reviewed By:_______________
DNA Conc.(ng/ul):_________
Interpretation:___________
Failed lanes: ____________
Comments:________________
Gel Picture
PHOTO DOCUMENT
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 1 of 9
®
®
March 2015
Rev. No: 00
Well No.
ICB
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
515
430
430
430
430
515
515
515
430
515
430
430
430
430
430
515
430
430
430
515
430
515
515
430
220
210
185
130
160
170
210
90
130
260
210
100
185
130
105
205
110
175
135
110
155
130
160
155
180
225
215
185
300
13
14
15
16
23
24
Am pS
175
210
215
Well No.
Specific
Reaction
1
2
3
4
5
6
7
8
9
10
11
12
195
260
17
18
19
20
21
22
Well No.
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
ICB
430
430
430
515
430
430
430
430
430
430
430
430
430
430
430
430
430
430
430
430
430
430
430
430
210
165
155
130
90
115
100
115
190
145
185
165
120
170
120
105
215
125
180
240
95
110
260
190
195
180
175
245
155
190
180
205
220
300
245
285
270
37
38
39
62
Am pS
220
260
130
150
165
175
235
190
220
220
265
Well No.
Specific
Reaction
25
26
27
28
29
30
31
32
33
34
35
36
49
50
51
52
53
54
55
56
57
58
59
60
61
ICB
430
430
430
430
430
430
430
515
430
430
430
430
430
190
75
140
130
150
190
145
190
185
265
230
135
195
230
150
195
220
52
53
54
55
56
57
58
59
60
61
Am pS
41
42
43
44
45
46
47
48
NC
Well No.
40
190
275
Well No.
Specific
Reaction
49
50
51
62
‘ICB’ Internal Control Band,
‘AmpS’ Amplicon Size
Notes:
Product sizes are approximate. For detailed information, see the lot-specific Specificity Table and Interpretation Table.
This table is intended as a guide. For interpretation always use the Interpretation Table and/or Specificity Table.
HLA-specific PCR products shorter than 125 base pairs have a lower intensity and are less sharp than longer PCR
products.
Primer mixes 9, 24 and 32 may give rise to a lower yield of HLA-specific PCR product than the other DQB1*06 primer
mixes.
Primer mixes 16, 18, 19, 20, 31, 32, 40, 46 and 50 have a tendency to giving rise to primer oligomer formation.
Primer mixes 8, 9, 10, 11, 15, 16, 20, 28, 30, 31, 34, 38, 40, 41, 51 and 57 may have tendencies of unspecific
amplifications, most pronounced in primer mix 15.
The nucleotide sequence of codon 14 of the DQB1*06:05:02 allele is not yet known. If codon 14 is CTg, then the
DQB1*06:05:02 allele will retain its name and will be amplified by the primer pair in well No. 10. If the sequence of codon
14 is ATg, then DQB1*06:05:02 will be renamed to DQB1*06:09:02 (Steven Marsh personal communication), and will not
be amplified by the primer pair in well No. 10.
Primer mix 62 contains a negative control, which will amplify more than 95% of HLA amplicons as well as the amplicons
generated by the control primer pairs matching the human growth hormone gene. HLA-specific PCR product sizes range
from 75 to 200 base pairs and the PCR product generated by the HGH positive control primer pair is 430 base pairs.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
Page 2 of 9
®
March 2015
Rev. No: 00
1
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9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
34
2
3
2
2
3
3
21
24
4
4
6
5
4
4
23
25
30
6
23
25
30
23
25
25
30
30
5
5
7
8
9
29
7
8
8
8
8
8
8
9
9 10 11
? ?
13
9 ? ? 12
29
29
?
?
29
29
4
4
4
5
5
7
7
6
6
8
8
8
5
2
2
2
22
22
9
9
9
9
9
23
23
11
11
11
11
4
6
6
9
4
2
2
2
3
3
2
3
11
13
16
9
9
21
25
25
18
24
10
4
5
6
6
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
7
7
8
?
?
?
?
?
?
29
29 ?
29 ?
29
?
?
?
21
21
23
21
23
21 22
21
15
15
8
32
11
6
7
1
19
14
3
3
3
3
25
25
14
2
2
21
13
5
5
30
30
21
6
5
?
?
30
21
?
?
?
29 ?
?
?
?
?
?
9
23
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
32
?
?
?
?
?
?
?
?
32
?
?
32
32
32
32
?
?
?
32
?
?
?
?
?
?
?
?
?
?
?
32
31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
Negative Control
Well No.
DQB1 allele 1,2,3
*06:01:01, 06:01:0306:01:06, 06:01:0806:01:11
*06:01:02, 06:01:07,
06:01:12
*06:02:01-06:02:04,
06:02:06, 06:02:0806:02:17
*06:02:05
*06:02:07
*06:03:01-06:03:03,
06:03:05-06:03:06,
06:03:11-06:03:14
*06:03:04, 06:03:0806:03:10
*06:03:07
*06:03:15
*06:04:01, 06:04:0306:04:08
*06:04:02
*06:05:01
*06:05:02
*06:06
*06:07:01
*06:07:02
*06:08:01
*06:08:02
*06:09:01
*06:09:02
*06:09:03, 06:09:05
*06:09:04
*06:10
*06:11:01
*06:11:02-06:11:03
*06:12
*06:13:01-06:13:02
*06:14:01
*06:14:02
*06:15:01-06:15:02
*06:16
*06:17
*06:18:01
*06:19:01
*06:20
*06:21
Well No.
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
Worksheet
For In Vitro Diagnostic Use
Page 3 of 9
®
1
2
3
2
3
3
4
5
6
7
8
8
4
4
5
6
7
4
5
6
4
4
4
5
5
5
8
8
2
2
1
1
8
3
7
8
7
8
7
7
8
8
2
2
1
6
3
4
4
5
5
6
6
4
5
6
2
2
2
2
2
2
2
2
3
3
3
3
3
3
7
1
1
1
1
1
7
1
8
2
2
2
2
2
2
3
4
4
4
4
4
4
5
5
5
5
5
8
6
6
6
6
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33
11
13 14
21
29 ?
?
9
11
13
21
29 ?
?
17
21
?
?
18
21
?
?
19
29 ?
?
20
?
?
9
25
?
?
23
25 26
?
? 33
15
21
?
?
18
25
?
?
10
23
25
?
?
25
?
?
21
27
30
9
28 29
32
26
?
?
9
19
29
32
13
20 21
?
?
9
22
29
32
9
29 30
23
25
27
?
?
23
25
32
9
11
18
32
31
23
25
30 31
10
?
?
11
21 22
?
?
21
30 31
13
21
?
?
21
25
?
?
21
28
?
?
13
16
21
13
16
21
?
?
9
16
29
32
26
?
?
?
?
14
?
?
26
?
? 33
27
?
?
9
29 ?
?
23
25
?
?
25
?
?
23
25
?
?
25
?
?
23
25
?
?
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33
34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
37
47
Negative Control
March 2015
Rev. No: 00
Well No.
*06:22:01
*06:22:02
*06:23
*06:24
*06:25
*06:26N
*06:27:01-06:27:02
*06:28
*06:29
*06:30
*06:31
*06:32
*06:33, 06:1144
*06:34
*06:35
*06:36
*06:37
*06:38
*06:39
*06:40
*06:41
*06:42
*06:43
*06:44
*06:45
*06:46
*06:47
*06:48
*06:49
*06:50
*06:51:01
*06:51:02
*06:52
*06:53
*06:54N
*06:55
*06:56
*06:57
*06:58
*06:59
*06:60
*06:61
*06:62
*06:63
Well No.
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
Worksheet
34
34
34 35
34
34
34
35
47 48
42
42
47
34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
For In Vitro Diagnostic Use
Page 4 of 9
®
1
1
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2
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2
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2
2
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3
3
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3
3
3
3
4
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8
7
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6
7
7
5
1
1
1
1
1
1
1
1
1
2
2
2
2
2
2
2
2
2
2
2
2
2
2
8
8
8
3
3
3
3
4
5
6
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
12
23
25
?
?
23
25
?
?
11
22
29 ?
?
23
25
?
?
44
21
?
?
43
9
13
15
21
29 ?
?
21
?
?
40
21
?
?
36
21
?
?
38
21
?
?
38 39
21
?
?
39
21
?
?
37
21
?
?
36 37
21
?
?
36
38
21
26
?
? 33
26
?
? 33
21
?
?
37
39
20 21
27
?
?
17
21
?
?
20 21
?
?
21
32
9 10
29 ?
?
9
29
32
46
23
25
30
47
9
11
29 30
9
29 ?
?
57
23
25
?
?
47
23
25
?
?
48
22 23
?
?
9
29 ?
?
42
11
29 ?
?
?
?
36
21
?
?
37
47
21
?
?
45
25
34
17
34
49
50
28
34
34
51
34
46
34
52
21
30
40
21
30
46
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
Negative Control
March 2015
Rev. No: 00
Well No.
*06:64
*06:65
*06:66
*06:67
*06:68
*06:69
*06:70, 06:75N4
*06:71
*06:72
*06:73
*06:74
*06:76
*06:77N
*06:78
*06:79:01
*06:79:02
*06:80
*06:81
*06:82
*06:83, 06:1254
*06:84
*06:85
*06:86
*06:87
*06:88
*06:89
*06:90
*06:91, 06:1284
*06:92
*06:93
*06:94
*06:95
*06:96
*06:97, 06:1244
*06:98
*06:99:01
*06:100
*06:101
*06:102N
*06:103
*06:104
*06:105
*06:106
*06:107
Well No.
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
Worksheet
For In Vitro Diagnostic Use
Page 5 of 9
®
1
1
2
2
2
2
2
2
2
2
2
3
4
5
6
4
5
6
8
3
3
3
3
3
3
3
4
1
7
5
8
2
8
2
2
2
2
3
3
3
8
1
2
2
2
4
4
5
5
6
6
7
1
2
2
2
2
2
8
3
3
3
4
5
6
8
2
4
4
5
5
6
6
4
5
6
3
3
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48
30
34
21
30
23
25
30
21
30
21
30
21
30
44
21
30
21
30
21
30
11
22
29
32
11
14
21
?
?
?
?
34
9
11
29 ?
?
42
21
?
?
41
15
?
?
38
21
?
?
21
30
9
12
32
13
19
21
30
41
?
?
43
27
?
?
34
23
25
?
?
23
25
?
?
9
29 ?
?
35
21
?
?
21
?
?
37 38
21
?
?
36
39
13
15
24
?
?
37
?
?
34
23
25
?
?
9
11
?
?
42
23
25
?
?
44
47
23
25 26
?
?
48
14
21
?
?
21
?
?
23
25
30
41
49 50 51 52 53 54 55 56 57 58 59 60 61 62
53
53
51
54
55
56
59
50
49
58
Negative Control
March 2015
Rev. No: 00
Well No.
*06:108
*06:109
*06:110
*06:111
*06:112N
*06:113
*06:115
*06:116
*06:117
*06:118:01
*06:119
*06:120
*06:121
*06:122
*06:123
*06:126
*06:127
*06:129
*06:130
*06:131
*06:132
*06:133
*06:134, 06:144N4
*06:135
*06:136
*06:137
*06:138
*06:139
*06:140
*06:141
*06:142
*06:143
*06:145
*06:146
*06:147
*06:148
*03:04:02, 03:10:01,
03:12, 03:14:02, 03:70
*03:08, 03:137
*03:23
*03:30, 03:72, 03:100
Well No.
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
Worksheet
54
49
52
50
57
61
49 50
60
53
59
34
47
5
1
2
3
4
5
6
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
7
8
13
24
37
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
For In Vitro Diagnostic Use
Page 6 of 9
®
March 2015
Rev. No: 00
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
46
53
15
24
37
w
24
w
w
w
3
21
37
24
?
5
?
17
Negative Control
Well No.
*03:97
*03:115
*03:132
*04:01:01-04:02:04,
04:02:06-04:03:02,
04:06-04:08, 04:1204:14, 04:16-04:22
*04:02:05, 04:11, 04:15
*04:09
*04:10
*05:03:02
*05:03:12
*05:04
*05:14, 05:31, 05:46
*05:38
*05:47
*05:62
Well No.
Visit www.olerup-ssp.com for
“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
Worksheet
?
51
15
1
2
3
4
5
6
7
8
24
52
15
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
1
HLA-DQB1 alleles listed on the IMGT/HLA web page 2014-April-14, release 3.16.0, www.ebi.ac.uk/imgt/hla.
Alleles that have been deleted from or renamed in the official WHO HLA Nomenclature up to and including the last IMGT/HLA database release can be
retrieved from web page http://hla.alleles.org/alleles/deleted.html.
2
3Primer
mix 8: Specific PCR fragment of 90 bp in the DQB1*06:65 allele. Specific PCR fragment of 175 bp in the DQB1*06:04:01-06:07:02, 06:09:01-06:09:03, 06:09:05,
06:18:01, 06:22:02, 06:25, 06:27:01-06:27:02, 06:32, 06:34, 06:36, 06:38-06:39, 06:52, 06:58, 06:66, 06:69, 06:85-06:86, 06:88-06:89, 06:93-06:94, 06:118:01, 06:121,
06:129, 06:135 and 06:142 alleles.
Primer mix 12: Specific PCR fragment of 100 bp in the DQB1*06:64 allele. Specific PCR fragment of 180 bp in the DQB1*06:06 allele. Specific PCR fragment of 215 bp
in the DQB1*06:06? and 06:129 alleles.
Primer mix 13: Specific PCR fragment of 185 bp in the DQB1*06:10 and 06:130 alleles. Specific PCR fragment of 225 bp in the DQB1*06:05:02, 06:15:01-06:15:02,
06:22:01-06:22:02, 06:37, 06:48, 06:51:01-06:51:02, 06:69 and 06:139 and the DQB1*03:30, 03:72 and 03:100 alleles.
Primer mix 14: Specific PCR fragment of 130 bp in the DQB1*06:09:04, 06:13:01-06:13:02, 06:22:01, 06:55 and 06:119 alleles. Specific PCR fragment of 215 bp in the
DQB1*06:146 allele.
Primer mix 15: Specific PCR fragment of 105 bp in the DQB1*06:14:01-06:14:02 and 06:69 and the DQB1*05:38 and 05:62 alleles . Specific PCR fragment of 185 bp in
the DQB1*06:29, 06:123 and 06:139 and the DQB1*03:132 alleles.
Primer mix 16: Specific PCR fragment of 205 bp in the DQB1*06:16 and 06:51:01-06:51:02 and the DQB1*04:01:01w-04:03:02w and 04:06w -04:22w alleles. Specific
PCR fragment of 300 bp in the DQB1*06:52 allele.
Primer mix 20: Specific PCR fragment of 110 bp in the DQB1*06:37 and 06:125 alleles. Specific PCR fragment of 210 bp in the DQB1*06:26N and 06:81 alleles. Specific
PCR fragment of 260 bp in the DQB1*06:83 allele.
Primer mix 22: Specific PCR fragment of 130 bp in the DQB1*06:07:01-06:07:02, 06:15:01-06:15:02, 06:46, 06:66, 06:92 and 06:118:01 alleles. Specific PCR fragment
of 195 bp in the DQB1*06:38 allele.
Primer mix 25: Specific PCR fragment of 210 bp in the DQB1*06:03:01-06:03:15, 06:08:01-06:08:02, 06:14:01-06:14:02, 06:27:01-06:28, 06:30-06:32, 06:40-06:41,
06:44, 06:59-06:65, 06:67, 06:87, 06:90-06:91, 06:98, 06:110, 06:128, 06:133-06:134, 06:141, 06:143-06:145 and 06:148 alleles. Specific PCR fragment of 260 bp in the
DQB1 *06:49 allele.
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DQB1*06
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Primer mix 26: Specific PCR fragment of 165 bp in the DQB1*06:35, 06:53 and 06:145 alleles. Specific PCR fragment of 190 bp in the DQB1*06:28, 06:56 and 06:79:0106:79:02 alleles.
Primer mix 27: Specific PCR fragment of 155 bp in the DQB1*06:114 allele. Specific PCR fragment of 195 bp in the DQB1*06:40, 06:81 and 06:132 alleles.
Specific PCR fragment of 220 bp in the DQB1*06:57 alleles. Specific PCR fragment of 265 bp in the DQB1*06:33 allele.
Primer mix 28: Specific PCR fragment of 130 bp in the DQB1*06:102N allele. Specific PCR fragment of 180 bp in the DQB1*06:50 allele. Specific PCR fragment of 300
bp in the DQB1*06:34 allele.
Primer mix 31: Specific PCR fragment of 100 bp in the DQB1*06:44 and 06:47 alleles. Specific PCR fragment of 220 bp in the DQB1*06:43 allele.
Primer mix 35: Specific PCR fragment of 185 bp in the DQB1*06:54N and 06:135 alleles. Specific PCR fragment of 260 bp in the DQB1*06:06? and 06:58 alleles.
Primer mix 37: Specific PCR fragment of 120 bp in the DQB1*06:80 allele. Specific PCR fragment of 175 bp in the DQB1*06:29, 06:76-06:77N, 06:96 and 06:139 and
the DQB1*03:30, 03:72, 03:100, 03:132 and 04:09 alleles. Specific PCR fragment of 245 bp in the DQB1*06:05:02 and 06:137 alleles.
Primer mix 38: Specific PCR fragment of 170 bp in the DQB1*06:78 and 06:123 alleles. Specific PCR fragment of 245 bp in the DQB1*06:137 allele. Specific PCR
fragment of 285 bp in the DQB1*06:72 and 06:73 alleles.
Primer mix 39: Specific PCR fragment of 120 bp in the DQB1*06:80 allele. Specific PCR fragment of 155 bp in the DQB1*06:138 allele. Specific PCR fragment of 270
bp in the DQB1*06:73 and *06:74 alleles.
Primer mix 40: Specific PCR fragment of 105 bp in the DQB1*06:70 allele. Specific PCR fragment of 190 bp in the DQB1*06:75N and 06:106 alleles.
Primer mix 42: Specific PCR fragment of 125 bp in the DQB1*06:93 allele. Specific PCR fragment of 165 bp in the DQB1*06:121 and 06:142 alleles. Specific PCR
fragment of 190 bp in the DQB1*06:60-06:61 alleles.
Primer mix 44: Specific PCR fragment of 130 bp in the DQB1*06:113 allele. Specific PCR fragment of 175 bp in the DQB1*06:67 allele. Specific PCR fragment of 220 bp
in the DQB1*06:143 allele.
Primer mix 45: Specific PCR fragment of 150 bp in the DQB1*06:97 allele. Specific PCR fragment of 235 bp in the DQB1*06:124 allele.
Primer mix 47: Specific PCR fragment of 95 bp in the DQB1*06:29, 06:59, 06:63, 06:87 and 06:96 and the DQB1*03:08 and 03:137 alleles. Specific PCR fragment of
180 bp in the DQB1*06:90 allele. Specific PCR fragment of 220 bp in the DQB1*06:143 allele.
Primer mix 48: Specific PCR fragment of 110 bp in the DQB1*06:59, 06:91 and 06:145 alleles. Specific PCR fragment of 205 bp in the DQB1*06:128 allele.
Primer mix 49: Specific PCR fragment of 190 bp in the DQB1*06:100, 06:132 and 06:140 alleles. Specific PCR fragment of 230 bp in the DQB1*06:126 allele.
Primer mix 50: Specific PCR fragment of 75 bp in the DQB1*06:134 allele. Specific PCR fragment of 150 bp in the DQB1*06:101 allele. Specific PCR fragment of 190 bp
in the DQB1*06:140 and 06:144N. Specific PCR fragment of 275 bp in the DQB1*06:120 allele.
Primer mix 52: Specific PCR fragment of 130 bp in the DQB1*06:133 alleles. Specific PCR fragment of 195 bp in the DQB1*06:105 and the DQB1*05:47 alleles.
Primer mix 53: Specific PCR fragment of 150 bp in the DQB1*06:109- 06:110 and the DQB1*03:115 allele. Specific PCR fragment of 220 bp in the DQB1*06:147 allele.
4The
following DQB1*06 alleles can be distinguished by the different sizes of the HLA-specific PCR product:
Alleles
Primer mix
Alleles
Primer mix
DQB1*06:33, 06:114
DQB1*06:70, 06:75
DQB1*06:83, 06:125
27
40
20
DQB1*06:91, 06:128
DQB1*06:97, 06:124
DQB1*06:134, 06:144N
48
45
50
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
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®
March 2015
Rev. No: 00
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“Instructions for Use” (IFU)
and detailed Product Inserts
DQB1*06
101.212-24/04 -24u/04u
93X
Worksheet
The DQB1*06 subtyping kit cannot distinguish the silent mutations in the DQB1*06:01:01, 06:01:03-06:01:06 and 06:01:08-06:01:11 alleles, the DQB1*06:01:02, 06:01:07 and
06:01:12 alleles, the DQB1*06:02:01-06:02:04, 06:02:06 and 06:02:08-06:02:17 alleles, the DQB1*06:03:01-06:03:03, 06:03:05-06:03:06 and 06:03:11-06:03:14 alleles, the
DQB1*06:03:04 and 06:03:08-06:03:10, the DQB1*06:04:01 and 06:04:03-06:04:08 alleles, the DQB1*06:09:03 and 06:09:05 alleles, the DQB1*06:11:02-06:11:03 alleles,
the DQB1*06:13:01-06:13:02 alleles, the DQB1*06:15:01-06:15:02 alleles or the DQB1*06:27:01-06:27:02 alleles.
“?”, nucleotide sequence information is not available for the primer matching sequence.
‘w’, might be weakly amplified.
Olerup SSP AB
Franzengatan 5
SE-112 51 Stockholm
Sweden
For In Vitro Diagnostic Use
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