Laboratory Specific
Chemical:
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan .
Refer to instructions for assistance.
_____________________________________________________________________________
Department_____________________________ Date when SOP was written: ___________
Date when SOP was approved by the lab supervisor: ____________________________________
Principal Investigator: _____________________________________________________________
Internal Laboratory Safety Coordinator/Lab Manager: __________________________________
Laboratory Phone:_______________ Office Phone:__ _______ _________________________
Emergency Contact:_____ _______________________________________________________
(Name and Phone Number)
Location(s) covered by this SOP: __________________ _______________________________
(Building/Room Number)
_____________________________________________________________________________
Process Hazardous Chemical Hazardous Class
( State the procedure the specific chemical is used for in lab/the purpose of the chemical )
Chemical Name: Evans Blue Dye
Synonyms: Direct Blue 53; 6,6[(3,3’-Dimethyl[1, 1’-biphenyl]-4,4’-diyl)bis[4-amino-5-hydroxy-1,3naphthalenedisulfonic acid] tetrasodium salt
CAS#: 314-13-6
Class: Irritant and Carcinogen
Molecular Formula: C
34
H
24
N
6
O
14
S
4
Na
4
1

Molecular weight: 960.82 g
Form: solid; dark brown powder
Solubility:
Volatility:
No data available in MSDS
Stable under recommended storage conditions. Store in a cool dry area. May be combustible at high temperatures.
Other: Laboratory operations should be conducted in a ducted fume hood as the chemical, physical and toxicological properties of Evans Blue Dye have not been thoroughly investigated.
Toxic Effects: Toxic effects are not available for Evans Blue Dye. However, it is considered a hazardous irritant and potential carcinogen.
Acute Effects:
Chronic Effects:
May cause respiratory tract irritation (wheezing, coughing, shortness of breath). May be harmful if swallowed (burning in the mouth or throat).
May cause eye irritation (watering or burning). May be harmful if absorbed through skin and cause skin irritation.
Evans Blue Dye contains a component that has been reported to potentially be carcinogenic. Prolonged exposure is not known to aggravate medical conditions. Overexposure may cause reproductive disorders based on tests with laboratory animals.
Carcinogenic Effects: Evans Blue Dye contains a component that has been reported to probably be carcinogenic.
Mutagenic/Teratogenic Effects: Evans Blue Dye is not known to be mutagenic or teratogenic.
Systemic effects: Not available
Wear gloves, full-length lab coat, and safety glasses with side-shields or a face shield in addition to long pants and closed-toe shoes.
Always wash hands after removing gloves following handling Evans Blue Dye.
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Always handle Evans Blue inside a certified chemical fume hood or ducted bio-safety cabinet.
Skin & Eye Exposure: Skin: remove contaminated clothing and wash gently and thoroughly with running water and non-abrasive soap. Be particularly careful to clean folds, crevices, and creases. Cover irritated skin with an emollient. If irritation persists, seek medical attention. Wash contaminated clothing before reusing. Eye: check for and remove any contact lenses. Rinse immediately with copious amounts of running water for at least 15 minutes. Do not use any ophthalmic ointments. Consult a physician promptly.
Ingestion: Do not induce vomiting. Loosen any tight clothing. Never give anything by mouth to an unconscious person. Rinse mouth with water and consult a physician.
Inhalation: Remove rapidly to clean air. Administer rescue breathing if necessary
and call emergency services. Seek medical attention if needed.

Keep away from heat and/or sources of ignition. Discard empty containers. Store away from extreme heat and strong oxidizing agents.

Needles used for Evans Blue Dye injection will be disposed of in approved sharps containers immediately following use.

Needles used for Evans Blue Dye injection should never be bent, sheared, or recapped.

In case of eye exposure: go to eyewash station and rinse eyes for 15 minutes, then seek medical attention

If inhaled: remove to fresh air, seek medical attention immediately

If absorbed: through skin, immediately flush with plenty of water, remove contaminated shoes and clothing, seek medical attention immediately

If ingested: Do NOT induce vomiting, loosen tight clothing, and seek medical attention immediately.
911
– Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors.
Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small area
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using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
– If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
– Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.
– Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.
– Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.
911
– Dial 911 (or 310-
825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
– Go to the Occupational Health Facility (OHF), x56771, CHS room 67-120 (This is on the 6 th floor, 7 th corridor, room 120. Enter through the School of Dentistry on
Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111 . Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
(as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing
231 from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F,
8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111 . Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.
Clean areas where Evans Blue Dye has been handled by adding water, followed with a soap and water wash.
No waste streams containing Evans Blue Dye shall be disposed of in sinks or general refuse. Extra Evans
Blue Dye is considered as hazardous chemical waste and disposed of in accordance with UCLA hazardous waste protocol. Absorbent materials (e.g., associated with spill cleanup) grossly contaminated shall be handled in accordance with the chemical waste disposal system.
(State the location of MSDS)
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(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
(signature of all users is required)
 Prior to conducting any work with Evans Blue Dye, designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.
 The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the Evans Blue Dye MSDS provided by the manufacturer.
 The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last two years.
I have read and understand the content of this SOP:
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