Agilent Technologies 1200 Series Analytical HPLC
The Agilent Technologies 1200 series HPLC was first released in 2007. It is an upgrade of the
1100 series HPLC consisting of upgrades to XXXX……………….
Comparison between the Agilent 1200 and Varian 920-LC
Compared to the Varian 920-LC the Agilent 1200:
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Standard autosampler cannot accept wellplates and the microplate 1200 series
autosampler cannot accept 1.6 mL HPLC vials. The Varian 920-LC autosampler is
capable of injecting from 1.6 mL vials, 96 well microplates or 384 well microplates.
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Is limited to 100 HPLC vials in the autosampler compared to 200 x HPLC vials in the
920-LC
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The standard 1200 series autosampler has a large 300 L delay volume compared to
the 200 L on the Varian 920-LC automated sample handler.
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Variable wavelength UV Vis detector is a single  detector only. The Varian 920-LC
has dual  capabilities with real-time purity checking.
Agilent claims the 1200 series quaternary pump
Offers flexibility for solvent selection and automated method development and is ideally suited
to the multi-method high throughput laboratories.
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Provides convenient front access to all maintenance parts for quick replacement
without having to disassemble the instrument
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Early Maintenance Feedback (EMF) continually tracks long term usage with predefined limits and feedback messages if the limit is exceeded
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Full programming capability such as intelligent method sequencing which includes
built-in automated shutdown methods to reduce solvent costs.
Note for the Agilent 1200 series quaternary pump system to work properly a 1200 series 4
channel vacuum degasser is required. To safely mount the solvents, a
What Agilent says about the 1200 series Autosampler
The standard 1200 series autosampler holds only 100 industry-standard vials.
Claimed features of the 1200 Series Autosampler are:
 Reproducible injections from 0.1 to 100 L
 Carryover typically < 0.1% without needle wash and < 0.05% using needle wash
 Overlapped injections to improve sample throughput
The 1200 series high performance autosampler has the following extra capabilities
 2 x 96 (or 384) wellplate capacity
 2 x 54 position HPLC vial racks
 Reduced delay volume for working with fast gradients by bypassing the injection
valve after an injection is made.
What Agilent says about the SSI Chromatography Data Software
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It’s quick and easy to store and retrieve chromatography data using an embedded,
relational database.
Take advantage of more precise peak integration calculations with ApexTrack™
algorithm.
Easily control Waters ACQUITY UPLC™ and Alliance® HPLC systems as well as
third-party HPLC and GC systems.
Enhanced detection capabilities are available when using Waters mass detectors or
the Waters 2996 Photodiode Array Detector.
Scalable, modular architecture allows seamless growth from a single workstation to
an enterprise-wide system.
Designed for a data-secured regulatory lab environment – three clicks to compliance.
What Agilent say about the 1200 Series VWD
Agilent claim a simplified optics design is optimized for high sensitivity and low baseline drift
 Programmable wavelength selection for sensitivity and selectivity
 Minimum baseline noise and maximum stability for lowest detection limits
 A wide linear range of 2 AU for simultaneous quantitation of major and minor
compounds
 A choice of flow cells including
o 10 mm path length 12 L dead volume and 40 bar pressure rating
o 4 mm path length 1 L dead volume and 40 bar pressure rating
o High pressure 12 L volume 10 mm, 400 bar pressure rated path length flow
cell
 Holmium oxide filter for automatic wavelength verification
The 1200 Series VWD has a D2 lamp only and wavelength range limited to a maximum of
600 nm, severely limiting its capabilities in the visible wavelength range. It’s 13 Hz data
acquisition rate limits it use for high resolution high throughput type applications. A noise
specification of + 7.5 x 10-6 Au for the 1200 Series VWD is poorer than the + 5 x 10-6Au of the
UV Vis detector in the 920-LC which ensures superior sensitivity performance of the 920-LC.
What Agilent Say about the 1200 Series DAD
Dimensions
Width
Height without detector
Depth
Weight
Compositional Precision
Agilent 1200 Series HPLC
580 mm
570 mm
(770 mm including 2487 UV
detector)
600 mm
68 Kg with 2487 UV detector
0.2% SD at 0.2 and 1.0
mL/min
Varian 920-LC
730 mm
700 mm
560 mm
<0.2% SD at 1.0 mL/min
Pressure Pulsations
< 2% of amplitude with
standard delay volume
<5% of amplitude with low
delay volume option
Gradient Delay Volume
Between 800 L and 1100 L