) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
University of Baghdad
College Name
College of Science
Department
Biology Department
Full Name as written
in Passport
Dhuha Saad Salih Jeryo
e-mail
dhuha.saad@yahoo.com
Career
Assistant Lecturer
Lecturer
Master
Thesis Title
Year
Abstract
Assistant Professor
Professor
PhD
Antigenic relationship between some Non agglutinable Vibrios isolated from
Iraqi water & biotype elator ( Inaba ,Ogawa) by precipitation in gel.
1977
Eight Iraqi water isolates of Nonagglutinable Vibrios were studied
biochemically & serologically. It was found that all of these isolates complied
to Sakazaki's definition of Nonagglutinable Vibrios (Sakazaki et al, 1967). It
was also found that the isolated No. 208 agglutinate with polyvalent OI serum
whilst isolate No. 139 did not. The two isolates ( 208 & 139) were chosen for
comparison of their antigenic component with Inaba & Ogawa by
agglutination & gel precipitation.
Cross agglutination tests showed that isolate No.208 agglutinate to a
titre of 80 with anti Inaba antiogawa sera prepared locally, while anti 208
agglutinated Inaba & Ogawa to a titre of 640.
No agglutination was observed when isolate No. 139 was titrate with
anti Inaba & anti ogawa sera. The reverse was true.
Crude soluble extract were obtained from isolated 208 , 139 & from
Inaba & ogawa. These extracts were studied by gel precipitation in agarose.
Precipitation test & absorption of various antisera with homologous
organisms revealed the presence of at least ten to twelve soluble antigens in
Inaba & Ogawa extract ( of these seven to nine were surface). Isolate No 208
contained nine soluble antigens (seven were surface) & isolate No. 139
contained seven soluble antigens ( five were surface).
Cross precipitation tests and absorption of various antisera with
heterologous organisms revealed the presence of three surface antigenic
components between Inaba & Ogawa one of them might be flagellar, two of
them were identical in their antigenic determinants. Cross precipitation tests
revealed the presence of two to three surface antigenic component between
Inaba & isolate No. 208 one of them might be flagellar , two of them were
identical in their antigenic determinant, and the test revealed the presence of
at least four surface antigenic components shared between Ogawa & isolate
No.208, one of them might be flagellar and two of them at least were identical
in their antigenic determinants.
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
Cross precipitation tests showed that no surface antigenic component
was found to be shared between isolate No .139& Inaba , Ogawa & isolate No.
208.
Cross agglutination tests & cross precipitation tests before & after
absorption of various antisera with live culture of biotype Inaba , ogawa &
isolate 208 revealed the presence of at least one identical surface antigenic
component between Inaba , ogawa , 208 whilst isolate 139 did not give any
similarity in surface antigens with the above three vibrios.
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
University of Baghdad
College Name
College of Science
Department
Biology Department
Full Name as written
in Passport
Dhuha Saad Salih Jeryo
e-mail
dhuha.saad@yahoo.com
Career
Assistant Lecturer
Lecturer
Master
Thesis Title
Year
Abstract
Assistant Professor
Professor
PhD
Study of Enteric Campylobacter Isolated from Diarrheal cases in children
1993
Campylobacter was isolated at "Saddam's central Hospital for children" from
the faeces of children with diarrhea . Specimens were watery or bloody
mucous . Twelve isolates were provisionally identified according to shape ,
motility, Gram stain and oxidase and Catalase tests. Three species were
identified by biochemical examination of four local isolates. These were
C.jejuni (2 isolates), C.coli (one isolate) and a third most probably new species
which morphologically and biochemically simulate C.laridis . The incidence of
thermophilic campylobacter was 10% in a total number of 120 specimens .
Electron microscopy of one isolate of Campylobacter revealed spiral , S or
comma shaped amphitrichous organisms with single polar flagellum . Local
isolates and a reference C.jejuni isolate were subjected to electrophoretic
study of 5 iso enzymes (malic enzyme ,malate dehydrogenase hexokinase ,
glucose phosphate isomerase and alkaline phosphatase ) .Results showed the
possibility of using malic enzymes and malate dehydrogenase in the
differentiation between the species of Campylobacter .
Proteins of the outer membranes for the 4 local isolates and the reference
strain were prepared by ultrasonication followed by ultra-centrifugation and
sarkosyl treatment. Thus a crude as well as sarkosyl soluble and sarkosyl
insoluble preparations were obtained , Polyacrylamide gel electrophoresis
revealed the presence of 16-87 kd proteins in the purified outer membrane
protein preparations.
An antiserum against one isolate of C.jejuni was prepared in rabbits using
formalin treated suspension .
Gel precipitation of this antiserum with the crude outer membrane protein of
the homologous and heterologous isolates as well as the reference strain
showed the presence of at least two lines of precipitation with homologous
and one line with heterologous antigens with the exception of C.coli which did
not give any line of precipitation. Complement fixation test of this antiserum
with the same antigenic preparation gave an antibody titer of 2560-5120.
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
Local hybrid chicks of 24-72 hrs.of age were chosen as test laboratory animals
to study the infectivity and pathogenicity of Campylobacter . Results showed
30% infectivity and clinical features terminated by death .C.coli caused watery
diarrhea in these birds while the two C.jejuni isolates and C.laridis as well as
the reference strain caused clear bloody diarrhea . Histopathological
examinations of intestinal cross sections showed damage in the lower small
intestine and the colon. The bacteria were reisolated from the intestine of the
infected chicks using selective media. Their presence in the colon
outnumbered that in the small intestine .Furthermore , some isolates were
seen in the spleen and livers of the infected chicks which indicated systemic
infections. This is the first report of a laboratory animal giving typical clinical
and histopathological pictures of the infection simulating those that occur in
humans.
The minimum infective dose was measured according to the presence of
clinical features of the affected chicks. Results show that 10 4 of the local
isolates of C.jejuni was capable of causing infection while 105 cells for the
reference strain is required . It was therefore concluded that local chicks are
very useful laboratory animal for studying the pathogenesis of Campylobacter.