FISH-ID 101 – LAB OBJECTIVES: To identify some of the common fishes located in Southwestern PA To identify some of the common fish shapes that fishes possess To identify some of the scale types present on various fish families To identify the various fish fin types present on fishes BACKGROUND: A variety of background information has been provided for your reference. Please refer to it for descriptions of scale, fin, and morphology descriptions. Also refer to the dichotomous key to assist with the identification of selected PA fishes. METHOD: There are a variety of fish specimens scattered throughout the lab. Your assignment today is to identify these specimens, providing a brief explanation as to why this fish is what it is on the attached data sheet. In the column marked, reason for ID provide a short rationale for why you called this fish what you did – i.e., what is/are the distinguishing characteristics. To say because it looks like this, isn’t enough. Also, examine all dissected specimens in the lab for both internal and external morphology. Be sure to note what the structure and function is of each part labeled. FISH-ID 101 – LAB Data sheet Name: Specimen # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 Family Name Common Name Reason for ID An introduction to fish shape, morphology, and identification Basic Fish Body Shapes: Sagittiform: "Arrow-like." Example shown is a grass pickerel. Other fish with this body type include pikes, gars, topminnows, killifish, needlefish, and barracuda. These body shapes are good for rover predators, which depend on an ability to strike quickly--often from a hiding place. Depressiform: "Flattened." Flounders, halibut, rays, and skates all have this form, which allows the fish to rest on the bottom and hide either using camouflage or by covering itself with a layer of sand. In streams, sculpins come close to having this shape. Fusiform: Streamlined and shaped like an airplane fuselage. The example shown is a blue-fin tuna. Tunas, mackerel, swordfish, sailfish, and marlin are all fusiform fishes. This shape allows them to move through the water extremely fast. In streams, most minnows have fusiform shapes. Compressiform: Compressed laterally. Example shown is a green sunfish. This shape is highly versatile and is probably the most common fish shape. It combines the advantages of several of the other fish body shapes. Many freshwater fishes have this shape, such as bass, crappie, and sunfish. Anguilliform: "Eel-like." Many eels, of course have this shape. Shown is a brook lamprey. This shape allows a fish to enter and hide in very narrow openings, and also helps the fish resist the force of current. Fish Morphology: Fin type: Spines: Spines are a late development in fishes, and many fish have them. Spines may be found almost anywhere on the body, and are used primarily for defense. Spines may have painful poisons in them, such as those of the catfish, or simply be annoying because they are sharp and serrated. Some fishes have developed large spines that merely make it difficult for other fishes to get their mouths around them, or impale the unfortunate diner. The most common locations for spines are the first part of the dorsal fin (or in fishes with two dorsal fins, the first dorsal fin), and the first part of the pectoral fins. Spines may be counted and their location noted, and this often helps us tell one species from another. Spines may also be cut and their rings counted to provide the fish's age, much like a forester would age a tree trunk. Pectoral fins: Pectoral fins may be horizontal and down low, like in a salmon, trout, shark, or sturgeon, and used mainly for gliding. Over the course of millennia they have moved upwards on the body of many fishes. Most reef and schooling fishes have their pectoral fins up high and oriented vertically, as in the above diagram. These are often are used for swimming, holding position, and changing directions quickly. Some fishes have further specialized their pectoral fins; the sculpin uses its broad, spiny pectoral fins to help it hold itself to the bottom against strong currents. Flying fishes have lengthened their pectoral fins to allow them to glide. Some fishes use only their pectoral fins to swim, sculling them through the water like oars. Pelvic fin location: Pelvic fins are usual abdominal, meaning that they are attached midway down the belly. Salmon and trout have pelvic fins at this location. When the pelvic fins are below the pectoral fins, such as can be seen in the diagram of the non-existent fish above, they are termed thoracic. When a thoracic pelvic fin is attached under the gills, it may also be called jugular, and if under the chin or eye, mental. General caudal fin shapes: A. The homocercal (homo-SUR-kul) tail is a modern development. It is symmetrical. It includes truncate, square, slightly forked, and deeply forked types. It is by far the most common caudal fin shape, shared by most fishes. B. The heterocercal tail is an ancient form, possessed by only a few primitive fishes, such as sharks, sturgeon, and paddlefish. It was a necessary tail shape when fishes had no swim bladders and were heavy in the front; if the fish tried to use a symmetrical tail, it would have plunged toward the bottom. Instead, it developed a tail with a deliberately downward-driving design and supplemented it with horizontal, plane-like pectoral fins that transformed that downward force into a horizontal, forward-driving force. Genius! C. This tail has a non-differentiated caudal fin. This may be found on eels of all sorts, as well as lampreys. D. The lunate tail is a tail that the tuna and mackerel have refined to an engineer's delight. This tail provides powerful forward force, with very little turbulence. Fish Scale Shapes: Ganoid scales are found in fishes such as the bichirs (Polypteridae), Bowfin (Amia calva), paddlefishes (Polyodontidae), gars (Lepisosteidae), and sturgeons (Acipenseridae). Ganoid scales are usually rhomboid in shape and have articulating peg and socket joints between them. They are modified cosmoid scales, which consist of a bony basal layer, a layer of dentine, and an outer layer of ganoine (an inorganic bone salt). Placoid scales are found in sharks and rays, and can vary greatly in external appearance. They do not increase in size as the fish grows, instead new scales are added. Cycloid and ctenoid scales are found in the majority of bony fishes (the Teleostei). The anterior part of each scale is usually overlapped by the posterior portion of the scale in front, which gives the fish great flexibility. Ctenoid scales have a variously developed spiny posterior margin (the word "ctenoid" is from the Greek word "cteno", meaning comb, and refers to the comb-like ctenii on the margin of the scale). Cycloid and ctenoid scales consist of two main regions, a surface "bony" layer, composed of an organic framework impregnated largely with calcium based salts, and a deeper fibrous layer composed mainly of collagen. These scales are derived from ganoid scales, which have lost the ganoine and thinned the bony embedded plate. As a fish with cycloid or ctenoid scales grows, its scales also grow. This results in a pattern of concentric growth rings on the scale, which look a little like the growth rings in the trunk of a tree. These are sometimes used to determine the age of the fish. cycloid ctenoid Key to Selected Families of Pennsylvania Fishes 1a. Head with jaws; no pelvic fins; body long and “snakelike”: Eel Family - Anguillidae. 1b. Pelvic fins present; body not long nor “snakelike”. (2) 2a. Upper portion of caudal (tail) fin base longer than lower portion. (3) 2b. Upper and lower portions of caudal fin base about equal in length. (5) 3a. Vertebrae extend into elongated upper portion of caudal fin; snout with four barbels on underside; body with separate rows of bony plates: Sturgeon family - Acipenseridae. 3b. Vertebrae not noticeably extended into upper portion of caudal fin; tail somewhat rounded. (4) 4a. Jaws long and thin with many teeth; scales thick and bony; dorsal fin short: Gar family - Lepisosteidae. 4b. Jaws short and thick; dorsal fin very long: Bowfin family - Amiidae. 5a. Adipose fin present. (6) 5b. Adipose fin not present. (7) 6a. Body scaleless with chin barbels present: Catfish family - Ictaluridae. 6b. Body with small scales: no chin barbels; body distinctly spotted; thin pointed flap of skin (axillary process) at base of pelvic fins: Trout and salmon family - Salmonidae. 6c. Body with large scales; no axillary process at base of pelvic fins: Smelt family - Osmeridae. 7a. Stout duck-bill snout and jaws with obvious sharp teeth; single dorsal fin with no spines: Pike family Esocidae. 7b. Not with duck-bill snout; dorsal fin single or double with both spines and soft rays or soft rays only. (8) 8a. Single soft-rayed dorsal fin completely forward of anal fin; mid-belly scales sharp and “saw-toothed”; silvery body strongly flattened from side to side; no lateral line: Herring family - Clupeidae. 8b. Single or double dorsal fin; lateral line present; “saw-toothed” mid-belly scales absent; body not strongly flattened from side to side. (9) 9a. Single soft-rayed dorsal fin (include carp and goldfish which have first ray hardened); one or more large pharyngeal (throat) teeth on separated fifth gill arches. (10) 9b. Dorsal fin single or double with both spines and soft rays; pharyngeal teeth present or absent (freshwater drum has teeth on fused pharyngeals). (11) 10a. Mouth directed downward with thick fleshy lips; distance from back edge of gill flap to beginning of anal fin much greater than distance from beginning of anal fin to base of tail fin; pharyngeal teeth more than 15 and in single row: Sucker family - Catostomidae. 10b. Mouth not with thick fleshy lips; distance between back edge of gill flap to beginning of anal fin only slightly greater than distance from beginning of anal fin to base of tail fins; pharyngeal teeth no more than 6 in one to three rows: Minnow family - Cyprinidae. 11a. Body largely scaleless (small prickles present); large pectoral fins; head broad and flattened: Sculpin family - Cottidae. 11b. Body with scales. (12) 12a. Anal fin with 3 or more spines. (13) 12b. Anal fin with 1 or 2 spines. (14) 13a. Gill cover with spine: Temperate bass family - Moronidae. 13b. Gill cover without spine: Sunfish family - Centrarchidae. 14a. Lateral line ending at base of caudal fin: Perch family - Percidae. 14b. Lateral line continues to end of caudal fin; soft-rayed portion of dorsal fin longer than spiny portion; large flat teeth present on heavy, fused lower pharyngeals; Drum family - Sciaenidae. Description of Selected Fish Families of PA Lampreys, Petromyzontidae: Lampreys lack the typical jaws of other fishes, in both their larval and adult forms. Larval lampreys have a sort of oral hood. In parasitic lampreys, after metamorphosis the hood is replaced by the adult’s concave, circular, sucker-disk mouth, with horny teeth. In nonparasitic lampreys, the hood does not change. The patterns of the teeth help biologists differentiate among lamprey species. Lampreys also do not have pectoral or pelvic fins, which are found on most other fishes. The lamprey’s thin, cylindrical body is eellike or snakelike. It has a single, low, long dorsal fin. The dorsal fin may be notched, but it is never divided in two in lampreys, and it connects to the curved tail fin. Lampreys have no body scales, and on the inside they have a poorly developed skeleton of cartilage. Most lampreys are gray, olive or brown. Some species have plain sides; others are mottled. The adult lamprey’s eyes are small. Larval lampreys have no readily visible eyes, although they are present, covered and sightless. Behind the lamprey’s eyes, on either side, is a row of roundish gill openings. Sturgeons, Acipenseridae: Biologists say that sturgeons are the most primitive of the bony fishes alive today. Extinct sturgeon relatives date back more than 350 million years. Sturgeons are found throughout the Northern Hemisphere, in both fresh water and salt water. In some parts of the world, sturgeons are commercially valuable for their meat and for their eggs, which are used for caviar. Although they were once a more common part of our native wildlife, nowadays Pennsylvania sturgeons are rare. Sturgeons are elongate, with a flattened head and snout, and seem to be five-sided. This is because they have five rows of bony plates running along the body, one along the back, two on the sides and two on the underside. The plates are sharp in young fish, but smooth with wear in older, larger fish. Sturgeons have four soft barbels between the front of the snout and the toothless mouth, which protrudes to pick up food. The upper lobe of the tail fin is much longer than the lower lobe, and the dorsal fin is set far back toward the tail. The first ray of the pectoral fin is hardened into a bony spine. It is used by biologists to determine the fish’s age. Paddlefish, Polyodontidae: The paddlefish’s most distinctive feature is its snout, or rostrum. This “paddle” is longer than the rest of the head and flattened on top and bottom, like a paddle. The snout is thought to be an organ of touch. It may also act as a stabilizer, keeping the fish swimming in a horizontal position when its jaw is extended downward during filter-feeding. Paddlefish have many long gill rakers that let them strain tiny food organisms from the water. Like the sturgeon, the paddlefish tail’s upper lobe is longer than its lower lobe, but it has no bony scales or plates on its body. The skeleton is mostly cartilage. The paddlefish’s gill cover extends backward in a long, flat point. The body color is generally medium to pale blue-gray. Young paddlefish don’t have the adult’s paddle-shaped snout. Young paddlefish also have teeth, which the adults lack. Gars, Lepisosteidae: Gars are primitive, ancient bony fish. Their ancestors date back more than 100 million years, as found in fossil records. In Pennsylvania, there are currently two gar species, the spotted (Lepisosteus oculatus) and the longnose (Lepisosteus osseus). At one time the shortnose gar was reported to be in the Pennsylvania portion of the Ohio River and in Lake Erie, but it has not been seen there in many years. The gar has a long, relatively thin-looking, cylindrical body that is “armored” with large, thick, diamond-shaped scales. Its beaklike or swordlike snout is filled with fine, sharp teeth. The single dorsal fin and the anal fin are located far back toward the tail. The spotted gar is usually olive-green on its back and silvery-white on the belly. There are large, roundish dark spots on the top and sides of the head, and on the upper part of the body. The fins have dark spots and may display orange tints. The spotted gar grows almost to four feet long. Longnose gar are grayish to olive-green on the back and white on the belly. They may have dark spots or blotches on their fins and especially toward the rear along their sides. The fins may show yellow or orange tints. They can grow to about 50 inches long. Bowfin, Amiidae: Bowfins are robust-looking fish. The body is covered with heavy scales and the head is covered with bony plates. Their color is olive-green on the back, lighter and mottled on the sides, and yellowish on the belly. A single long, low dorsal fin extends over half the length of the body. It is light with lengthwise stripes. The dorsal fin is just barely separated from the broadly rounded tail fin, which shows curving stripes. A pair of short barbels protrudes near the nostrils. The mouth is filled with sharp, strong teeth. Male bowfins can be identified by the dark spot on the upper side of the base of the tail fin. This spot is ringed with bright-orange or yellowgreen during the breeding season. This spot may represent a false eye that could direct predators to the bowfin’s tail, instead of the head, thus providing it with an escape mechanism. Bowfins grow to several feet long and nearly 10 pounds. Mooneyes, Hiodontidae: In appearance both are quite similar to herrings, having adipose eyelids, scaleless heads, cycloid scales and a prominent axillary process. They are distinguished from herrings by the lack of scutes, the presence of a lateral line, prominent teeth and the dorsal fin is inserted directly above the base of the anal fin. Both species are seldom caught or observed by anglers. Eel, Anguillidae: Freshwater eels are the only catadromous fishes in North America. “Catadromous” means that they spawn in salt water and live as adults in fresh water. Anadromous fishes, like salmon and American shad, spawn in fresh water but live as adults in the ocean. On this continent, eels are represented by a single species, the American eel (Anguilla rostrata). Although the eel looks snakelike, it is a fish. The American eel’s body is long and slender, and seems scaleless. Actually, it has smooth, tiny scales that are embedded in the skin. A long, low dorsal fin extends over at least two-thirds of the back. It blends with the caudal fin and the anal fin, which is also long and low, on the underside. There are no pelvic fins, but the pectoral fins are well-developed. The presence of pectoral fins can be used to distinguish an eel from a lamprey, which has no paired fins. The head has a smallish eye. The head is long, and tapers to a small mouth. The lower jaw sticks out a little farther than the upper jaw. Eels are yellowish brown to dark-olive, and lighter underneath. In Pennsylvania, the maximum size is two to three feet, although four feet or more is possible. Females grow larger than males. Herrings, Clupeidae: The herrings have several distinct physical features that separate them from the other fishes: a single row of sawtooth scales called scutes occur along the belly, scaleless head, transparent eye covering that is termed an adipose eyelid, and an auxiliary process that is located at the base of the pelvic fin. Minnows, Cyprinidae: Most small fishes, regardless of species, are erroneously called "minnows", which leads to misidentification. Cyprinids are small in size, rarely reaching more than 12 inches in length -- even as adults. Not all members of the minnow family are small, however; fishes such as carp, goldfish, and white amur attain a size which ranks with the largest freshwater fishes. Cyprinids share several common taxonomic characters, which separate them from the other fish families. External features include: scaleless head, toothless jaws, lack of adipose fin, lack of appendages at the base of the pelvic fins, and a single, soft dorsal fin in native species that has less than 10 rays. Internal anatomical features are: cyprinids have fewer than 10 teeth in any row on the pharyngeal arch, an enlarged intestine instead of a true stomach, and a series of bones called weberian ossicle that form a rudimentary ear. The list of freshwater fishes in the United States and Canada contains 754 species, of which the largest group is Cyprinidae, and has 221 species in 43 genera. Suckers, Catostomidae: Worldwide distribution of the sucker family is confined mainly to North America except for two or perhaps three species that are indigenous to Asia. All fishes in the sucker family possess pharyngeal teeth in the throat, which are tooth-like structures used for crushing food. Many suckers are identified by the configuration of these teeth. Some species have thin, comb-like pharyngeal teeth for filtering plankton directly from the water like Carpiodes and Ictiobus. Others, such as Moxostoma, Catostomus and Cyceleptus, have strong, flat-topped, molar-like structures that are used for crushing and grinding mollusks and crustaceans. Suckers, as their name implies, are mainly bottom feeders, foraging by sucking up materials from the bottom, separating out the indigestible detritus and ejecting it through the mouth and operculum. At other times some members of the family, mainly the buffalofishes, filter plankton for food directly from the water. The mouth of all suckers is located on the underside of the head and is tipped with fleshy protrusile lips. All family members are soft-rayed fishes with toothless jaws, scaleless heads, cycloid scales -- smooth-edged --, forked caudal fin, and a single, continuous, fleshy dorsal fin. Many suckers look like and are often confused with minnow species, but they differ in many features. Most of the suckers have 10 or more dorsal fin rays, which is always one or two more than the native minnows. Catfishes, Ictaluridae: Family members can be separated by the simplest criteria into three major groups: the large catfishes that include channel catfish, flathead catfish and blue catfish, all of which often reach weights of over 20 pounds; the bullheads, including black, yellow and brown, which rarely exceed 4 pounds in weight; and the madtoms, represented by the tadpole madtom, slender madtom, stone cat and freckled madtom, which are the smallest of the catfishes. Catfishes are easily distinguished from the other fishes by their smooth scaleless bodies, eight elongated fleshy barbels or "whiskers" abouth their mouth, and the strong, sharp spines that are located at the insertion of the dorsal and pectoral fins. It is beived that the spines are adapted as defensive structures in the catfish family. A locking mechanism allows the fish to extend the spine outward when attacked or touched, making it hazardous for a predator to grasp it and nearly impossible to swallow. Madtoms and small bullheads have glands at the base of the spines that secrete a mild but painful venom when danger is threatened. Pikes, Esocidae: The body is round-shaped and elongated with a frontal-flattened head that has a duck-bill shaped jaw which is lined with large, canine-like teeth. The dorsal fin is inserted far back on the body. Identification of individual pike species is another matter that demands close observation of key characters that separate one from another. Mudminnows, Umbridae: The banded killifish has an elongated body with flattened sides and large, round scales. Both males and females have dark vertical lines on the sides. Lines near the tail are shorter than those on the body. The back is olive, yellowish olive, or brownish yellow, fading to yellowish white or silvery-white on the bottom. The fins are light-olive or yellowish olive. Killifish have no lateral line, and the tail is squarish. The banded killifish has rows of small, sharp teeth in the upper and lower jaw. Adults are usually two to four inches long. Trouts, Salmonidae: Trout and salmon have a fleshy lobe, called the adipose fin, between the dorsal fin and the tail. Their scales are small and cycloid, or smooth, and embedded in a slimy mucous that is most obvious in the salmons. Trout and salmon have an obvious lateral line, large mouth and teeth. In big specimens, the teeth are caninelike. The tail may be forked or squarish, depending on the species, and none of the fins has spines. Mature males look different from females because they develop a long, hooked lower jaw, called a “kype.” Mature males also deepen or gain in color at spawning time. Coloration in trout and salmon varies from dull to intense, according to the species, where the fish lives and the time of year. Trout and salmon that live in the sea or a large lake become silvery. Silversides, Atherinidae: Silversides are distributed from tropical to temperate waters. Two widely separated dorsal fins, the first with flexible spines and the second with one spine followed by soft rays; anal fin with one spine followed by soft rays; pectoral fins high on body; mouth small and terminal; no lateral line; broad silvery lateral band (black in preserved specimens); pelvic fins usually abdominal; scales relatively large Sculpins, Cottidae: Sculpins are small, camouflaged fish reaching four or five inches in length. Their dark-and-light mottled color pattern helps them hide on the stream bottom. The broad head, fleshy mouth and upward-peering eyes look large for the rest of the body. Sculpins are compressed top to bottom, tapering quickly from a robust head to a narrow tail. The large, fanlike pectoral fins and the sculpin’s flattened body shape allow it to stay pressed against the stream bottom, maintaining its position when in swift water–a hydrodynamic adaptation. Sculpins have no swim bladder, so they are nonbuoyant and move over the bottom in short spurts. There are two dorsal fins. The front fin is spiny and the rear one is soft. Both are held erect. The pelvic fins have a single spine and soft rays. The sculpin’s body is scaleless, except for some scattered areas that have small, sharp scales called “prickles.” The tail is straight or rounded. Temperate basses, Percichthydae: The fishes in this family were historically referred to as the sea basses, since most of the members were saltwater inhabitants, except for a few species that were found in freshwater environments. Most ichthyologists acknowledge that all of the temperate basses originated in the sea and that some became landlocked during geological land-mass changes. For that reason it was proposed and adopted by fish taxonomists that the Percichthyidae family be expanded to include all temperate freshwater basses along with the giant sea basses and the ocean striped bass. Sunfishes, Centrarchidae: All are characterized by having at least one spine at the front part of the dorsal fin, which is continuous with the rear portion. Their body is deeply compressed laterally, and the attachment of the pelvic fins is far forward, nearly beneath the pectoral fins. There are three or more spines at the front of the anal fin, and the scales are ctenoid, which means they have rough edges. Perches, Percidae: The perch family includes the yellow perch, walleye, sauger and many darter species. Members of the perch family are characterized by rather slender, elongated bodies and by a large bone on the gill cover which ends in a flat spine. The dorsal fins are a very distinctive characteristic of the family with a definite separation evident between the anterior spiny portion and the soft portion to the posterior. The mouth of walleye and sauger is filled with formidable canine teeth on the jaws, the roof of the mouth and palate, teeth that are absent in the perch and darters. All members of the family are strictly carnivorous. The large species are piscivorous, eating mostly other fishes, while the smaller darter species prey mostly on minute aquatic insects and planktonic crustaceans. A wide range of forage and habitat preference is primarily responsible for the distribution of the family throughout Iowa. Drums, Sciaenidae: The freshwater drum (Aplodinotus grunniens) is Pennsylvania’s only freshwater representative of a large group of 210 marine species found in temperate and tropical coastal waters around the world. The lower pharyngeal (throat) arches are heavy and fused, and they bear flat, molar-like teeth. The spiny and soft-rayed portions of the dorsal fin are narrowly joined. The spiny-rayed portion is shorter that the spinous section. The lateral line continues to the end of the tail fin. In Pennsylvania, the freshwater drum occurs in Lake Erie, and in the Allegheny, Ohio and Monongahela rivers. The freshwater drum is dark-green to olive-brown on the upper part of the head, back and sides, shading to silvery on the sides. The belly is white. The anal fin has two spines, the first, very small and the second, quite large. The body is sharply arched. IDENTIFICATION OF THE TEMPERATE BASS White Bass, Striped Bass and their Hybrid Yellow bass can be identified because they do not have a tooth patch on their tongue and the second spine of the anal fin is longer than the base of the anal fin. White bass can be identified because they have a single center tooth patch, and their stripes are faint. On a white bass, the first stripe below the lateral line is not distinct nor complete to tail. Some striped bass may have broken lines, but the stripes of a fresh hybrid are distinct and definitely broken. The shape of the head can be a distinguishing characteristic between the striped bass and the hybrid bass (wiper).