Rita Chen
BIOGRAPHICAL SKETCH
Rita P.-Y. Chen 陳佩燁
Date of birth
24 August 1967
Work address:
Institute of Biological Chemistry, Academia Sinica
Tel:
02-2785-5696
Fax:
02-2788-9759
Email:
pyc@gate.sinica.edu.tw
Nationality:
Taiwan, ROC
Sex:
F
Status:
Married
Languages:
Chinese (Mandarin, Taiwanese), English, and French
Education and Experience:
1985-1989
1989-1991
1992-1993
1993-1998
1999-2003:
2003-2010
2004-now
2004-now
2010-now
B. S.
Department of Agricultural Chemistry, National Taiwan
University
M. Sc.
Institute of Biochemical Sciences, National Taiwan University
Research Assistant
Institute of Biological Chemistry, Academia Sinica
Ph. D.
Department of Biochemistry, University of Cambridge, UK
Postdoctoral Fellow
Institute of Chemistry, Academia Sinica
Assistant Research Fellow
Institute of Biological Chemistry, Academia Sinica
Adjunct Assistant Professor
Institute of Biochemical Sciences, National Taiwan University
Adjunct Assistant Professor
Institute of Life Science, National Defense Medical Center
Associate Research Fellow
Institute of Biological Chemistry, Academia Sinica
Awards
2010 Academia Sinica Young Investigator Award 中央研究院年輕學者研究著作獎
Membership
Protein Society
Cambridge Philosophical Society
Biophysics Society of R.O.C.
Taiwan Magnetic Resonance Society
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Rita Chen
Journal Reviewer
European Journal of Biochemistry
Journal of the Chinese Chemical Society
Journal of Agricultural and Food Chemistry
African Journal of Microbiology Research
Journal of Biochemistry
FEBS letter
Publications
(A) Paper (2000-now)
(1)
Zerella, R., Chen, P. Y., Evans, P. A., Raine, A., and Williams, D. H. (2000)
Structural characterization of a mutant peptide derived from ubiquitin:
Implications for protein folding. Protein Sci. 9, 2142-2150.
(2)
Chen, P. Y., Gopalacushina, B. G., Yang, C. C., Chan, S. I., and Evans, P. A. (2001)
The role of a beta-bulge in the folding of the beta-hairpin structure in ubiquitin.
Protein Sci. 10, 2063-2074.
(3)
Chen, P. Y., Lin, C. K., Lee, C. T., Jan, H., and Chan, S. I. (2001) Effects of turn
residues in directing the formation of the beta-sheet and in the stability of the
beta-sheet. Protein Sci. 10, 1794-1800.
(4)
Chen, P. Y., Lin, C. C., Chang, Y. T., Lin, S. C., and Chan, S. I. (2002) One
O-linked sugar can affect the coil-to-beta structural transition of the prion peptide.
Proc. Natl. Acad. Sci. USA 99, 12633-12638.
(5)
Chen, R. P. Y. (2004) The history and new progress of prion diseases. J. Gen. Mol.
Biol. 15, 88-95.
(6)
Chen, R. P. Y., Huang, J. J. T., Chen, H. L., Jan, H., Velusamy, M., Lee, C. T.,
Fann, W. S., Larsen, R. W., and Chan, S. I. (2004) Measuring the refolding of
-sheets with different turn sequences on a nanosecond time scale. Proc. Natl.
Acad. Sci. USA 101, 7305-7310.
(7)
Kuo, N. N., Huang, J. J., Miksovska, J., Chen, R. P., Larsen, R. W., and Chan, S. I.
(2005) Effects of turn stability on the kinetics of refolding of a hairpin in a
beta-sheet. J. Am. Chem. Soc. 127, 16945-16954.
(8)
Liang, F. C., Chen, R. P., Lin, C. C., Huang, K. T., and Chan, S. I. (2006) Tuning
the conformation properties of a peptide by glycosylation and phosphorylation.
Biochem. Biophys. Res. Commun. 342, 482-488.
(9)
Lee, L. Y., and Chen, R. P. (2007) Quantifying the sequence-dependent species
barrier between hamster and mouse prions. J. Am. Chem. Soc. 129, 1644-1652.
(10)
Isaza, C. E., Zhong, X., Rosas, L. E., White, J. D., Chen, R. P., Liang, G. F., Chan,
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Rita Chen
S. I., Satoskar, A. R., and Chan, M. K. (2008) A proposed role for Leishmania
major carboxypeptidase in peptide catabolism. Biochem. Biophys. Res. Commun.
373, 25-29.
(11)
Kao, M. W., Yang, L. L., Lin, J. C., Lim, T. S., Fann, W., and Chen, R. P. (2008)
Strategy for efficient site-specific FRET-dye labeling of ubiquitin. Bioconjug.
Chem. 19, 1124-1126.
(12)
Chen, R. P., Liang, F. C., Lee, C. T., Zerella, R., and Chan, S. I. (2008)
Contributions of a surface hydrophobic cluster to the folding and structural
stability of ubiquitin. J. Chin. Chem. Soc. 55, 772-781.
(13)
Ho, C. C., Lee, L. Y., Huang, K. T., Lin, C. C., Ku, M. Y., Yang, C. C., Chan, S. I.,
Hsu, R. L., and Chen, R. P. (2009) Tuning the conformational properties of the
prion peptide. Proteins: Struct. Func. Bioinform. 76, 213-225.
(14)
Chang, E. S., Liao, T. Y., Lim, T. S., Fann, W., and Chen, R. P. (2009) A new
amyloid-like -aggregate with most of the amyloid characteristics except fibril
morphology. J. Mol. Biol. 385, 1257-1265.
(15)
Hsu, R. L., Lee, K. T., Wang, J. H., Lee, L. Y., and Chen, R. P. (2009)
Amyloid-degrading ability of nattokinase from Bacillus subtilis natto. J. Agri.
Food. Chem. 57, 503-508.
(16)
Chang, C. C., Huang, T. Y., Cook, C. E., Lin, G. W., Shih, C. L., and Chen, R. P.
(2009) Developmental expression of Apnanos during oogenesis and
embryogenesis in the parthenogenetic pea aphid Acyrthosiphon pisum. Int. J. Dev.
Biol. 53, 169-176.
(17)
Tsai, W.-B., Chen, R. P.-Y., Wei, K.-L., Chen, Y.-R., Liao, T.-Y., Liu, H.-L., and
Lai, J.-Y. (2009) Polyelectrolyte multilayer films functionalized with peptides for
promoting osteoblast functions. Acta Biomaterialia 5, 3467-3477.
(18)
Lee, M. M., Isaza, C. E., White, J. D., Chen, R. P., Liang, G. F., He, H. T., Chan, S.
I., and Chan, M. K. (2009) Insight into the substrate length restriction of M32
carboxypeptidases: Characterization of two distinct subfamilies. Proteins 77,
647-657.
(19)
Chen, A. K., Lin, R. Y., Hsieh, E. Z., Tu, P. H., Chen, R. P., Liao, T. Y., Chen, W.,
Wang, C. H., and Huang, J. J. (2010) Induction of amyloid fibrils by the
C-terminal fragments of TDP-43 in amyotrophic lateral sclerosis. J. Am. Chem.
Soc. 132, 1186-1187.
(20)
Tsai, W.-B., Chen, R. P.-Y., Wei, K.-L., Tan, S.-F., and Lai, J.-Y. (2010)
Modulation of RGD-functionalized polyelectrolyte multilayer membrane for
promoting osteoblast function. J. Biomat. Sci. 21, 377-394.
(21)
Chen, P. T., Liao, T. Y., Hu, C. J., Wu, S. T., Wang, S. S., and Chen, R. P. (2010) A
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Rita Chen
highly sensitive peptide substrate for detecting two A-degrading enzymes:
Neprilysin and insulin-degrading enzyme. J. Neurosci. Methods 190, 57-62.
(22)
Huang, T. Y., Cook, C. E., Davis, G. K., Shigenobu, S., Chen, R. P., and Chang, C.
C. (2010) Anterior development in the parthenogenetic and viviparous form of the
pea aphid, Acyrthosiphon pisum: hunchback and orthodenticle expression. Insect.
Mol. Biol. 19 Suppl 2, 75-85.
(B) Patent
1. Chen, R. P. and Lee, K.T. Nattokinase for degrading and reducing amyloid fibrils
associated with Alzheimer’s disease, prion diseases, and other amyloidoses. USA
patent in application. Application no: 12618451. Filing date: November 13, 2009.
Taiwan patent in application. Application no: 098139802. Filling date: November
23, 2009. Japanese patent in application. Application no: 100130328. Filling date:
December 22, 2009.
2. Chen, R. P., Wang, S. S., and Hu, C.-J. Compositions and methods for detecting
amyloid--degrading enzyme activity. Provisional application No. is 61236457.
Filing date: August 24, 2009.
(C) Symposium
1.
Chen, P.-T., Liao, T.-Y., Wu, S.-T., Hu, C.-J., Wang, S. S.-S., and Chen, R.
P.-Y., A fluorogenic substrate for high-throughput screening compounds that
promote the production of the A-degrading enzyme neprilysin in cells. The 15th
Joint Biophysics Conference, Institute of BioMedical Sciences, Academia Sinica,
19-21 May, 2010
2.
Lin, C.–Y., and Chen, R. P.-Y., Design of a suicide peptide inhibitor for
Alzheimer’s Therapy. The 15th Joint Biophysics Conference, Institute of
BioMedical Sciences, Academia Sinica, 19-21 May, 2010
3.
Chuang, C.-C. and Chen, R. P.-Y., Exploring the sequence-dependent species
barrier of prion disease transmission. The 15th Joint Biophysics Conference,
Institute of BioMedical Sciences, Academia Sinica, 19-21 May, 2010
4.
Chen, P.-T., Liao, T.-Y., Wu, S.-T., Hu, C.-J., Wang, S. S.-S., and Chen, R.
P.-Y., A fluorogenic substrate for high-throughput screening compounds that
promote the production of the A-degrading enzyme neprilysin in cells. Joint 2010
Tohoku university-Taiwan Neuroscience Workshop for Young Scientists, The
Center for Traditional Arts, Forte Dong-Shan Villa, Taiwan, 21–23 January, 2010
4
Rita Chen
5.
Yang, L.-L.,Kao, M. W.-P.,Lim T.-S.,Chen H.-L., Fann, W. and Chen, R. P.-Y.,
Investigations on Ubiquitin by spFRET. Joint 2009 Annual Meeting Chemical
Society of ROC, ISU University, Taiwan, 4–6 December, 2009
6.
Chen, H.–L., Hsu, J. C.-C. and Chen, R. P.-Y., Studying the fast folding kinetics
of an antifreeze protein RD1 by using photolabile caging strategy and laser flash
photolysis. Joint 2009 Annual Meeting Chemical Society of ROC, ISU University,
Taiwan, 4–6 December, 2009
7.
Hsu, J. C.-C., Chen, H.-L., Liu, C.-H., Chen, S. S.-W., Chang, E. S.-H., Wang,
A. H.-J., Hsu, M.-F., Fann, Wunshain, Chen, R. P.-Y., Probing the Fast Folding
Kinetics: Folding Studies of an Antifreeze Protein RD1 by Using Photolabile
Caging Strategy and Laser Flash Photolysis. The 12th Society of Chinese
Bioscientists in America (SCBA) International Symposium, Taipei, Taiwan, 14 18 June, 2009
8.
Lee, C.-Y., Lim, T.-S. and Chen, R. P.-Y., Study of amyloid fibril formation of
full-length mouse prion protein and short mouse prion peptide. The 12th Society of
Chinese Bioscientists in America (SCBA) International Symposium, Taipei,
Taiwan, 14-18 June, 2009.
9.
Liao, T.-Y., Chang, E. S.-H. , Lim, T.-S., Fann, W.-S., and Chen, R. P.-Y., An
A
-like aggregate and is able to attenuate the
th
cytotoxicity of A . The 12 Society of Chinese Bioscientists in America
(SCBA) International Symposium, Taipei, Taiwan, 14–18 June, 2009
10. Hsu, R.-L., Lee, K.–T., Wang, J.-H., Lee, L. Y.-L., and Chen, R. P.-Y.,
Amyloid-degrading ability of nattokinase from Bacillus subtilis natto. The 12th
Society of Chinese Bioscientists in America (SCBA) International Symposium, ,
Taipei, Taiwan, 14–18 June, 2009
11. Yang, L.-L.,Kao, M. W.-P.,Lim T.-S.,Chen H.-L., Fann, W. and Chen, R. P.-Y.,
Investigations on Ubiquitin by spFRET. The 14th Joint Biophysics Conference ,
National Cheng Kung University, Tainan, Taiwan, 24–27 June, 2009
12. Chen, H.–L., Hsu, J. C.-C. and Chen, R. P.-Y., Studying the fast folding kinetics
of an antifreeze protein RD1 by using photolabile caging strategy and laser flash
photolysis. Joint International Conference of Biophysics and 14th Annual
Conference of the Biophysical Society of ROC, National Cheng Kung University,
Tainan, Taiwan, 24–27 June, 2009
13. Lee, C.-Y., Lim, T.-S. and Chen, R. P.-Y., Study of amyloid fibril formation of
full-length mouse prion protein and short mouse prion peptide. Joint International
Conference of Biophysics and 14th Annual Conference of the Biophysical
Society of ROC, National Cheng Kung University, Tainan, Taiwan, 24-27 June,
2009.
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Rita Chen
14. Lin, C.–Y., Liao, T.–Y., Chang, E. S.–H., Lim, T.–S., Fann, W.–S. and Chen, R.
P.-Y., Design of a suicide peptide inhibitor to trap A β 40 and attenuate its
cytotoxicity. The 14 th International Conference of Biophysics and Annual
Conference of the Biophysical Society of ROC, National Cheng Kung University,
Tainan, Taiwan, 24-27 June, 2009
15. Speaker, Frontier of Protein Aggregation and Neurodegenerative Diseases
Symposium, Taipei, Taiwan, 4-5 June 2009.
16. Speaker, NCTS March Workshop on Critical Phenomena and Complex
Systems, Taipei, Taiwan, 27, 30 March 2009.
17. Hsu, J. C.-C., Chen, H.–L. and Chen, R. P.-Y., Studying the fast folding kinetics
of an antifreeze protein RD1 by using photolabile caging strategy and laser flash
photolysis. The 6th Asian Biophysical Association (ABA) Symposium, Hong
Kong, China, 11–15 January, 2009
18. Lee, C. Y., and Chen, R. P.-Y., Study of fibril formation of full-length mouse prion
protein. The 6th Asian Biophysics Association (ABA) Symposium, Hong Kong,
China, 11-15 January, 2009
19. Speaker, The 9th Taiwan International Symposium on Statistical Physics, Taipei,
Taiwan, 8-12 July, 2008
20. Kao, M. W., Yang, L. L., Lin, J. C., Lim, T. S., Fann, W. and Chen, R. P.-Y.,
Strategy for efficient site-specific FRET-dye labeling of ubiquitin. The 9th Taiwan
International Symposium on Statistical Physics, Taipei, Taiwan, 8-12 July, 2008
21. Chang, E. S.-H., Liao, T.-Y., Lim, T. S., Fann, W. and Chen, R. P.-Y. An A40
mutant forms an amyloid-like aggregate and is able to attenuate the cytotoxicity of
A40. The 9th Taiwan International Symposium on Statistical Physics, Taipei,
Taiwan, 8-12 July, 2008
22. Hsu, J. C.-C. and Chen, R. P.-Y. Studying the folding kinetics of an antifreeze
protein RD1 by using photolabile caging strategy and laser flash photolysis. The 9th
Taiwan International Symposium on Statistical Physics, Taipei, Taiwan, 8-12
July, 2008
23. Kao, M. W., Yang, L. L., Lin, J. C., Lim, T. S., Fann, W. and Chen, R. P.-Y.,
Strategy for efficient site-specific FRET-dye labeling of ubiquitin. The 22nd
symposium of the protein society, San Diego, USA, 19-23 July, 2008
24. Liao, T.-Y., Chang, E. S.-H., Lim, T. S., Fann, W. and Chen, R. P.-Y. An A40
mutant forms an amyloid-like aggregate and is able to attenuate the cytotoxicity of
A40. The 22nd symposium of the protein society, San Diego, USA, 19-23 July,
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Rita Chen
2008
25. Speaker, The 13th Joint Biophysics Conference, Tai-jung, Taiwan, 21-24 May,
2008
26. Kao, M. W., Yang, L. L., Lin, J. C., Lim, T. S., Fann, W. and Chen, R. P.-Y.,
Strategy for efficient site-specific FRET-dye labeling of ubiquitin. The 13th Joint
Biophysics Conference, Tai-jung, Taiwan, 21-24 May, 2008
27. Chang, E. S.-H., Liao, T.-Y., Lim, T. S., Fann, W. and Chen, R. P.-Y. An A40
mutant forms an amyloid-like aggregate and is able to attenuate the cytotoxicity of
A40. The 13th Joint Biophysics Conference, Tai-jung, Taiwan, 21-24 May, 2008
28. Hsu, J. C.-C. and Chen, R. P.-Y. Studying the folding kinetics of an antifreeze
protein RD1 by using photolabile caging strategy and laser flash photolysis. The
13th Joint Biophysics Conference, Tai-jung, Taiwan, 21-24 May, 2008
29. Speaker, Bilateral Conference of University of California at Davis and
Academia Sinica Taiwan, Taipei, Taiwan, December 6 2007
30. Speaker, 2007 NCTS October Workshop on Critical Phenomena and Complex
Systems, Taipei, Taiwan, October 2007.
31. Speaker, Symposium of Structural and Biochemical Properties of Prions and
Amyloids, Taipei, Taiwan, October 2006.
32. Chang, E. S-H & Chen, R. P-Y (2006) Studying the sequence dependent amyloid
fibril formation. Prion 2006 Strategies, advances and trends towards protection
of society, Torino, Italy, Oct 3-6 2006
33. Lee, L. Y-L & Chen, R. P-Y (2006) Evaluation of risk factor of BSE and vCJD
transmission regarding human codon 129 polymorphism. Prion 2006 Strategies,
advances and trends towards protection of society, Torino, Italy, Oct 3-6 2006
34. Liu, C-H, Chen, S-L, Hsu, M-F, Yu, S. S-F, Chan, SI, Wang, A. H-J, Fann, W-S
& Chen, R. P-Y (2006) Studying the folding kinetics of an antifreeze protein RD1
using photolabile caging strategy and laser photolysis. The 11th Symposium on
Recent Advances in Biophysics, Taipei, Taiwan, May 24 – 26 2006
35. Chen, R. P-Y (2005) Effects of glycosylation and phosphorylation on amyloid
formation of the prion peptide. Prion Biology, Hinxton, UK, September 7-11
2005.
36. Ho, V. C-C, Lin, C-C & Chen, R. P-Y (2004) Effects of glycosylation and
phosphorylation on the conformational energy of the prion peptide and the
formation of amyloid fibril. 9th Symposium of the Biophysical Society of
7
Rita Chen
Taiwan, Taipei, Taiwan, May 2004.
37. Ho, V. C-C, Lin, C-C & Chen, R. P-Y (2004) Effects of glycosylation and
phosphorylation on the conformational energy of the prion peptide and the
formation of amyloid fibril. Meeting of the International and Asian-Pacific
Societies for Neurochemistry, Taipei, Taiwam, February 2004.
8