Program of Events
Tuesday, November 11
2:00 - 4:20 pm
Course registration: Marley Building Lobby, University of Arizona.
4:30 pm
Transportation to Temple of Music and Art.
5:00 - 5:30 pm
Mixer.
5:30 pm
Group Dinner sponsored by Telechem: Temple of Music and Art.
7:00 - 7:45 pm
Lecture 1. Introduction to the workshop and to microarrays (David
Galbraith): Temple of Music and Art.
8:00 pm onwards
Discussion of participants’ goals.
Wednesday, November 12
All further lectures and lab sessions will be in the Marley Building.
8:30 – 9:20 am
Lecture 2. Microarray production (David Galbraith).
9:20 - 9:45 am
Lecture 3. Involving statisticians in experimental design (Cheryl
Vanier; David Henderson).
9:45- 10:00 am
Coffee Break. Sponsored by Agilent.
10:00 – 12:30 pm
Lab session 1. (a) Target preparation (RNA isolation and
precipitation). (b) RNA amplification.
12:30 – 1:30 pm
Lunch sponsored by Becton-Dickinson.
1:30 – 3:00 pm
Lab session 2. (a) Target preparation (Quality checking by RNA gel
electrophoresis, and Quantification of RNA using
spectrophotometers). (b) RNA amplification (continued).
Demonstration of Bioanalyzer instrumentation
(Lenore Kelly).
1:30-3:00 pm
Demonstration of Microarray Printers (small groups to Marley room
814).
3:00 – 3:30 pm
Coffee Break. Sponsored by PerkinElmer Life and Analytical
Sciences.
3:30 ~ *6:30 pm
Lab session 3. (a) Target labeling. Array cross-linking and
hybridization demonstration. Demonstration of Hybridization
Workstations. (b) RNA amplification -- labeling of products.
Evening
Group Dinner (no host) at Gentle Ben’s.
Thursday, November 13
8:30-9:00 am
Genetix desktop microarray printer demonstration (Jim Bull).
Breakfast provided.
9:00 – 9:50 am
Lecture 4. Issues in experimental setup and hybridization
(Rangasamy Elumalai).
10:00 - 10:45 am
Lecture 5. Methods for amplifying microarray signals (Fang Cheng
Gong).
10:45 - 11:15 am
Coffee Break.
11:15 – 1:00 pm
Lab session 4. Target preparation (purification steps).
1:00 – 2:00 pm
Lunch sponsored by Amersham. Presentation on Amersham’s
amplification techniques.
2:00 – 2:45 pm
Lecture 6. Options in profiling platforms (Michael Deyholos,
University of Alberta).
2:45 ~ 5:30 pm
Lab session 5. Microarray cross linking and hybridization –
participants using their materials.
5:30 - 8:00 pm
Group Dinner at Marriott Hotel. Sponsored by Invitrogen.
8:30 pm
Lab session 5a. Put arrays to hybridize overnight.
Friday, November 14
8:30 am - 12:00 pm
Lab session 6. Microarray washing and scanning -- participants. Data
extraction and analysis -- participants.
After scanning, and before spot finding during lab 6: Lecture 7 on
spot finding/morphology/parameters issues (Cheryl Vanier).
10:15 – 10:45 am
Coffee break sponsored by Invitrogen. Group Photograph.
12:00 – 1:00 pm
Lunch sponsored by Biodiscovery.
1:15 - 2:15 pm.
Guest lecture: Dr. Robert Tempelman (Michigan State University).
“Statistical aspects of normalization and replication in microarray
experiments”.
2:15 – 3:00 pm
Lab session 7. Complete spot finding. Data extraction. Introduction
to Genesight; upload data.
3:00 - 3:45 pm.
Lecture 8. Statistical Issues I: Experimental design and data
analysis. (Cheryl Vanier).
2:15 - 5:00 pm
Demonstration of PerkinElmer Life and Analytical Sciences
microarray scanner (small groups).
3:45 - 4:15 pm.
Coffee break. Sponsored by PerkinElmer Life and Analytical
Sciences.
4:15 - 4:45 pm
Guest lecture: Todd Martinsky (Telechem, Inc.). “Microarray
manufacturing”.
4:45 - 7:00 pm.
Lab session 7: BioDiscovery: visualizing data, available normalization
and transformation procedures, links to annotation, and background
correction.
7:00 pm
Dinner on your own.
Saturday, November 15
9:00 – 10:00 am
Lecture 9. Statistical issues II: Clustering, and Bayesian statistics
for microarrays (David Henderson).
10:00 - 10:30 am
Lab session 8.1. Biodiscovery’s methods for differential expression
detection
10:30 - 11:00 am
Coffee break.
11:00 - 12:30 pm
Lab session 8.2. Statistical analysis of microarray data: Dave
Henderson’s software, SAS.
12:30 – 1:30 pm
Lunch sponsored by Biomicrosystems.
1:30 - 4:00 pm
Lab session 8.2. (continued) and 8.3. Comparison of results from
statistical analyses, multiple comparison issues.
4:00 - 5:00 pm
Course wrap-up (David Galbraith) and departures. Note: lab will
remain open as long as is required by the participants to complete
their tasks.