Kara Bren
Department of Chemistry · University of Rochester
Rochester, NY 14627-0216 USA
Office (585) 275-4335 · Fax: (585) 276-0205
email: bren@chem.rochester.edu
PUBLICATIONS
2010
Zinc Porphyrin as a Donor for FRET in Zinc Cytochrome c, Andrea J. Lee, Amy A. Ensign,
Todd D. Krauss, and Kara L. Bren, J. Am. Chem. Soc. 2010, 132, 1752-1753. DOI:
10.1021/ja909106p
NMR and DFT Investigation of Heme Ruffling: Functional Implications for Cytochrome c,
Matthew D. Liptak, Xin Wen, and Kara L. Bren, J. Am. Chem. Soc. 2010, 132, 9753–9763.
DOI: 10.1021/ ja102098p.
A Heme Fusion Tag for Protein Affinity Purification and Quantitation, Wesley B. Asher and
Kara L. Bren, Protein Science 2010, in press. DOI: 10.1002/pro.460 Variation and Analysis
of Second-sphere Interactions and Axial Histidinate Character in c-type Cytochromes, Sarah
E. J. Bowman and Kara L. Bren, Inorg. Chem. 2010, 49, in press.DOI:
10.1021/ic100899k
2009
Studies of Ferric Heme Proteins with Highly Anisotropic/Highly Axial Low Spin (S= 1/2)
Electron Paramagnetic Resonance Signals with Two Histidines or Histidine and Methionine
Axial Fe Coordination. Giorgio Zoppellaro, Kara L. Bren, Amy A. Ensign, Espen Harbitz,
Ravinder Kaur, Hans–Petter Hersleth, Ulf Ryde, Lars Hederstedt, and K. Kristoffer
Andersson, Biopolymers 2009, 91, 1064-1082.
2008
Modulation of the Ligand-Field Anisotropy in a Series of Ferric Low-Spin Cytochrome c
Mutants derived from Pseudomonas aeruginosa Cytochrome c-551 and Nitrosomonas
europaea Cytochrome c-552: A Nuclear Magnetic Resonance and Electron Paramagnetic
Resonance Study. Giorgio Zoppellaro, Espen Harbitz, Ravinder Kaur, Amy A. Ensign, Kara
L. Bren, and K. Kristoffer Andersson, J. Am. Chem. Soc. 2008, 130, 15348-15360. DOI:
10.1021/ja8033312
Methionine Ligand Lability of Type I Cytochromes c: Detection of Ligand Loss Using
Protein Film Voltammetry. Tao Ye, Ravinder Kaur, F. Timur Senguen, Lea V. Michel, Kara
L. Bren and Sean J. Elliott, J. Am. Chem. Soc. 2008, 130, 6682–6683. DOI:
10.1021/ja801071n
Zinc porphyrin: A fluorescent acceptor in studies of Zn-cytochrome c unfolding by
fluorescence resonance energy transfer. Amy A. Ensign, Iris Jo, Ilyas Yildirim, Todd D.
Krauss, and Kara L. Bren, Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 10779-10784. DOI:
10.1073/pnas.0802737105
The chemistry and biochemistry of heme c: functional bases for covalent attachment. Sarah
E. J. Bowman and Kara L. Bren, Nat. Prod. Rep.2008, 25, 1118-1130. DOI:
10.1039/b717196j
Native and Unfolded Cytochrome c - Comparison of Dynamics using 2D-IR Vibrational
Echo Spectroscopy. Seongheun Kim, Jean K. Chung, Kyungwon Kwak, Sarah E. J.
Bowman, Kara L. Bren, Biman Bagchi and M. D. Fayer, J. Phys. Chem. B , 2008, 112,
10054-10063. DOI: 10.1021/jp802246h
2007
Heme Attachment Motif Mobility Tunes Cytochrome c Redox Potential. Lea V. Michel, Tao
Ye, Sarah E. J. Bowman, Benjamin D. Levin, Megan A. Hahn, Brandy S. Russell, Sean J.
Elliott, and Kara L. Bren, Biochemistry 2007, 46, 11753-11760. DOI: 10.1021/bi701177j
Effects of Heme Pocket Structure and Mobility on Cytochrome c Stability. Xin Wen, Kirti
M. Patel, Brandy S. Russell, and Kara L. Bren, Biochemistry 2007, 46,2537-2544. DOI:
10.1021/bi602380v
2006
Cytochrome c552 Mutants: Structure and Dynamics at the Active Site Probed by
Multidimensional NMR and Vibration Echo Spectroscopy. Aaron M. Massari, Brian L.
McClain, Ilya J. Finkelstein, Andrew P. Lee, Heather L. Reynolds, Kara L. Bren, and Michael
D. Fayer, J. Phys. Chem. B 2006, 110, 18803-18810. DOI: 10.1021/jp054959q
2005
Redox Properties of Wild-Type and Heme-Binding Loop Mutants of Bacterial Cytochromes
c Measured by Direct Electrochemistry. Tao Ye, Ravinder Kaur, Xin Wen, Kara L. Bren,
and Sean J. Elliott, Inorg. Chem., 2005, 44, 8999-9006. DOI: 10.1021/ic051003l
Heme Axial Methionine Fluxion in Pseudomonas aeruginosa Asn64Gln Cytochrome c551.
Xin Wen and Kara L. Bren, Inorg. Chem. 2005, 44, 8587-8593. DOI: 10.1021/ic050976i
The Influence of Aqueous versus Glassy Solvents on Protein Dynamics: Vibrational Echo
Experiments and Molecular Dynamics Simulations. Aaron M. Massari, Ilya J. Finkelstein,
Brian L. McClain, Anne Goj, Xin Wen, Kara L. Bren, Roger F. Loring, and Michael D.
Fayer, J. Am. Chem. Soc. 2005, 127, 14279-14289. DOI: 10.1021/ja053627w
An Obligatory Intermediate in the Folding Pathway of Cytochrome c552 from
Hydrogenobacter thermophilus. Carlo Travaglini-Allocatelli, Stefano Gianni, Vikash K.
Dubey, Allesandro Borgia, Adele Di Matteo, Daniele Bonivento, Francesca Cutruzzolà, Kara
L. Bren, and Maurizio Brunori, J. Biol. Chem. 2005, 280, 25729-25734. DOI:
10.1074/jbc.M502628200
Suppression of Axial Methionine Fluxion in Hydrogenobacter thermophilus Gln64AsnCytochrome c552. Xin Wen and Kara L. Bren, Biochemistry 2005, 44, 5225-5233. DOI:
10.1021/bi047556+.
2004
Folding, Conformational Changes, and Dynamics of Cytochromes c Probed by NMR
Spectroscopy. Kara L. Bren, Jason A. Kellogg, Ravinder Kaur, and Xin Wen, Inorg. Chem.
2004, 43, 7934-7944. DOI: 10.1021/ic048925t
Cytochrome rC552, Formed During Expression of the Truncated, Thermus thermophilus
Cytochrome c552 Gene in the Cytoplasm of Escherichia coli, Reacts Spontaneously to Form
Protein-Bound, 2-Formyl-4-vinyl (Spirographis) Heme. James A. Fee, Thomas R. Todaro,
Eugene Luna, Donita Sanders, Laura Hunsicker-Wang, Kirti M. Patel, Kara L. Bren, Ester
Gomez-Moran, Michael G. Hill, Jingyuan Ai, Thomas M. Loehr, W. Anthony Oertling,
Pamela A. Williams, C. David Stout, Duncan McRee, and Andrzej Pastuszyn, Biochemistry
2004, 43, 12162-12176. DOI: 10.1021/bi048968l
Heme Axial Methionine Fluxionality in Hydrogenobacter thermophilus Cytochrome c552.
Linghao Zhong, Xin Wen, Terry M. Rabinowitz, Brandy S. Russell, Elizabeth F. Karan, Kara
L. Bren, Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 8637-8642. DOI:
10.1073/pnas.0402033101
2003
Backbone Dynamics and Hydrogen Exchange of Oxidized Pseudomonas aeruginosa
Ferricytochrome c551. Brandy S. Russell, Linghao Zhong, Maria Giulia Bigotti, Francesca
Cutruzzolà, and Kara L. Bren, J. Biol. Inorg. Chem. 2003, 8, 156-166. DOI: 10.1007/s00775002-0401-z
2002
Characterization of Recombinant Horse Cytochrome c Synthesized with the Assistance of
Escherichia coli Cytochrome c Maturation Factors. Jason A. Kellogg and Kara L. Bren,
Biochim. Biophys. Acta 2002, 1601, 215-221. DOI: 10.1016/S1570-9639(02)00471-5
Denaturant Dependence of Equilibrium Unfolding Intermediates and Denatured State
Structure of Horse Ferricytochrome c. Brandy S. Russell and Kara L. Bren, J. Biol. Inorg.
Chem. 2002, 7, 909-916. DOI: 10.1007/s00775-002-0381-z
Peptide Mimotopes of Pneumococcal Capsular PS of 6B Serotype. A Peptide Mimotope
Can Bind to Two Unrelated Antibodies. Jeon-Soo Shin, Jigui Yu, Jisheng Lin, Linghao
Zhong, Kara L. Bren, and Moon H. Nahm., J. Immunol. 2002, 168, 6273-6278.
http://www.jimmunol.org/cgi/reprint/168/12/6273
Characterization of Hydrogenobacter thermophilus Cytochromes c552 Expressed in the
Cytoplasm and Periplasm of Escherichia coli. Elizabeth F. Karan, Brandy S. Russell and
Kara L. Bren, J. Biol. Inorg. Chem. 2002, 7, 260-272. DOI: 10.1007/s007750100292
2001
Recombinant Cytochrome rC557 Obtained from Escherichia coli Cells Expressing a
Truncated Thermus thermophilus cycA gene: Heme Inversion in an Improperly Matured
Protein. Duncan E. McRee, Pamela A. Williams, Vandana Sridhar, Andrzej Pastuszyn, Kara
L. Bren, Kirti M. Patel, Ying Chen, Thomas R. Todaro, Donita Sanders, Eugene Luna, and
James A. Fee, J. Biol. Chem. 2001, 276, 6537-6544. DOI: 10.1074/jbc.M008421200
2000
Integrity of Thermus thermophilus Cytochrome c552 Synthesized by Escherichia coli Cells
Expressing the Host-Specific Cytochrome c Maturation Genes, ccmABCDEFGH:
Biochemical, Spectral and Structural Characterization of the Recombinant Protein. James A.
Fee, Ying Chen, Thomas R. Todaro, Kara L. Bren, Kirti M. Patel, Michael G. Hill, Ester
Gomez-Moran, Thomas M. Loehr, Jingyuan Ai, Linda Thöny-Meyer, Pamela A. Williams,
Enrico Stura, Vandana Sridhar, and Duncan E. McRee, Protein Sci. 2000, 9, 2074-2084
NMR Investigation of Ferricytochrome c Unfolding: Detection of an Equilibrium Unfolding
Intermediate and Residual Structure in the Denatured State. Brandy S. Russell, Rory
Melenkivitz, and Kara L. Bren, Proc. Natl. Acad. Sci. U.S.A. 2000, 97, 8312-8317. DOI:
10.1073/pnas.150239397