1 ENI Angela Obiageli Department of Biological Sciences, Covenant University, Canaan Land, KM 10, Idiroko Road, P.M.B. 1023 Ota, Ogun state, Nigeria Phone 234 (0) 8063939991 angela.eni@covenantuniversity.edu.ng, angelaeni@gmail.com EDUCATION University of the Witwatersrand, Johannesburg, South Africa, Ph. D. in Molecular and Cell Biology, November 2008 University of Nigeria, Nsukka B.Sc. (Honors) in Botany (Second Class upper division), March 1998 AWARDS Post-Doctoral Fellowship, National Research Foundation, South Africa, July, 2012-June, 2013. PhD Research Fellowship awarded by the International Institute of Tropical Agriculture (IITA) under the Gatsby Charitable Foundation UK Project GAT2261 (2004-2008). Award for Recognition of Outstanding Performance in three consecutive years (2001-2003), International Institute of Tropical Agriculture, (IITA) Ibadan, Oyo State, Nigeria (2003). Meritorious and Outstanding Service Award, Oyo State National Youth Service Corps (1999). WORKING EXPERIENCE Department of Biological Sciences, Covenant University, Ota, Nigeria Senior Lecturer, August 2013 - Date. Lecture, supervise seminar presentations, and assess the following post-graduate courses: MCB 812: Research Methodology, MCB 815: Advanced Immunology and Virology; MCB 822: Microbial Biotechnology; MCB 923: Advanced Virology II, MCB 926: Advanced Immunology II, BLY 911: Advanced Molecular Genetics. Lecture, compose, administer and grade assignments, tests and examinations for the following undergraduate courses: MCB 222: Techniques in Virology, MCB 316: Immunology, MCB 414: Virology, MCB 423: Medical Entomology (Arboviruses only), MCB 413: Microbial genetics. School of Molecular and Cell biology, University of the Witwatersrand, South Africa Post-Doctoral Fellow, July 2012 – June 2013. Successfully transformed starch-rich, industry-preferred cassava variety, T200, with an ACMV gene construct. Over 50 transformation events have been fully characterized and are ready for green-house trials. 2 Successfully designed the first trio-hybrid vector construct using genes from the three cassava mosaic begomoviruses reported to infect cassava in Southern Africa (ACMV, EACMV and SACMV). Transformation with this vector is aimed at achieving multiple virus resistance in industry preferred starch-rich cassava varieties in Southern Africa Completed molecular characterization of infected field samples from 2009 survey of farmer’s fields in Southern Nigeria. Department of Biological Sciences, Covenant University, Ota, Nigeria Lecturer I, September 2010 – July 2013. Lecture, supervise seminar presentations, and assess the following post-graduate courses: MCB 812: Research methodology and MCB 822: Microbial Biotechnology. Lecture, compose, administer and grade assignments, tests and examinations for the following undergraduate courses: MCB 222: Techniques in Virology, MCB 316: Immunology, MCB 414: Virology, MCB 423: Medical Entomology (Arboviruses only), MCB 413: Microbial genetics, MCB 417: Research methods in Microbiology. Supervised eleven (17) Honors final projects. Lecturer II, January 2009 - August 2010 Re-designed course structure for MCB 222: Techniques in Virology, MCB 316: Immunology, and MCB 414: Virology. Taught and assessed the following undergraduate courses: MCB 222: Techniques in Virology, MCB 316: Immunology, MCB 414: Virology, MCB 423: Medical Entomology (Arboviruses only), MCB 413: Microbial genetics, MCB 417: Research methods in Microbiology. Supervised six (6) Honors final projects. Surveyed and collected virus infected leaves from cultivated crops and associated weeds in Anambra, Delta, Edo, and Ondo states in Nigeria. Molecular testing of survey samples for Badnaviruses/Geminiviruses and investigation of the role of weeds as alternate hosts for these viruses was initiated. Virology Unit, International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria Senior Research Supervisor, July 2002 – December 2008 Served as the interim Head of the Virology Unit from March 2005 - July 2007, providing active and constant technical support, and supervision to a staff of 8 -10 and 5 post graduate students. Produced rabbit polyclonal antibodies for the detection of Dioscorea alata bacilliform virus (DaBV) and a yam isolate of Cucumber mosaic virus (CMV). These antibodies are currently being used for DaBV and CMV detection and monitoring across West Africa. Conducted surveys of major yam-producing agro-ecological zones in Ghana, Togo and Benin. Indexed yam leaves by ELISA, IC-PCR and/or IC-RT-PCR for five yam viruses and made first country-wide yam virus distribution maps for the three countries. Cloned, sequenced and analyzed the molecular diversity among yam-infecting badnavirus isolates from Ghana, Togo, Benin and Nigeria. 3 Liaised with IITA scientists and ensured timely completion of all virus indexing request to the Virology Laboratory. Coordinated short (one week to six months) virology training courses and made presentations on the goals and activities of the virology laboratory to IITA guests. Ensured timely purchase and prudent use of reagents and consumables in the laboratory, screen-houses, and animal house. Collated and prepared the Virology Laboratory contributions for the IITA annual reports. Research Supervisor, July 1999 - June 2002 Assisted with purification, antibody production and optimization of an ELISA diagnostic tool for the detection of Cytoplasmic polyhedrosis virus (CPV) in the larvae of Maruca vitrata. A useful diagnostic tool for the assessment of the use of CPV for the biological control of Maruca vitrata. Worked on molecular differentiation of isolates of Neozygites tanajoae, a fungal pathogen evaluated for use as a biological control agent of cassava green mites Mononychellus tanajao Promptly completed all external requests for scanning electron microscopy studies; studied the effect of microbial infection on the physiology of leaf samples, the effects of various physical and chemical treatments on starch grains from cassava, maize and coco-yam and the structural difference of different alloys of iron and clay. Completed ELISA virus indexing for breeders, tissue culture clean-up and certification of IITA mandate crops for international distribution. Conducted theoretical discussions and practical demonstrations of plant virology principles and techniques in various training courses for students, visiting scientists and IITA collaborators. Facilitated prompt procurement and adequate management of equipment, reagents and consumables for laboratory use. Assisted with travel arrangement, preparation of name tags and conference bags during the Plant Virology in Sub- Saharan Africa Conference hosted by the Virology Laboratory of IITA in June 2001. National Youth Service Corp, September1998 - July 1999 Carried out ELISA indexing of leaf samples for viruses infecting yam and cowpea. Assisted with purification of Dioscorea alata bacilliform virus (DaBV) and Yam mosaic virus (YMV). Implemented long-term calcium chloride preservation of plant virus isolates in virus-source plant materials. Successfully preserved the major viruses infecting IITA mandate crops (cassava, yam, cowpea, maize and Musa). This repository still serves as a source of reference materials till date. Contributed to mechanical, insect and seed transmission studies of viruses infecting cassava, yam and cowpea. 4 PUBLICATIONS BOOK 1. Eni, A. O. (2011). Viruses infecting yam in Ghana, Togo and Benin in West Africa: Epidemiology, Diagnostics and Molecular Studies. LAMBERT Academic Publishing, Germany. 151 pp. JOURNAL ARTICLES Plant Virology 2. Eni, A. O., Kumar, P. L., Asiedu, R., Alabi, O. J., Naidu, R. A., Hughes, J. d’A. and Rey, M. E. C. (2013). Characterization of Cucumber mosaic virus isolated from yam (Dioscorea spp.) in West Africa. African Journal of Biotechnology 12(22): 3472-3480. 3. Eni, A. O., Hughes, J. d’A., Asiedu, R. and Rey, M. E. C. (2013). Incidence and diversity of mixed viruses lower in yam tubers and tuber sprouts compared with field leaf samples: Implications for virus-free planting material control strategy. African Journal of Agricultural Research. 8(23): 3060-3067. 4. Eni, A. O., Ogunsanya P., Oviasuyi T. and Hughes, J. d’A. (2013). Alarming increase in the incidence of Cucumber mosaic virus in cowpea (Vigna unguiculata (l.) Walp.) in northern Nigeria. Archives of Phytopathology and Plant Protection 46(16):1958-1965. Thomson Reuters Journal 5. Eni, A. O. and Fasasi, D. K. (2013). Molecular detection of two cassava Begomoviruses in some parts of Southern Nigeria. African Journal of Agricultural Research 8(16):1350-1353. 6. Eni, A. O., Hughes, J. d’A., Asiedu, R. and Rey, M. E. C. (2012). Re-evaluation of yam mosaic virus (YMV) detection methods. Academic Journal of Plant Sciences 5 (1): 18-22 7. Eni, A. O., J. d’A. Hughes and M. E. C Rey (2011). Yam virus diseases: A threat to a food security crop in West Africa Phytopathology 101(6): S49-S49 Thomson Reuters indexed 8. Eni, A. O., Hughes, J. d’A. and Rey, M. E. C. (2011). Production of yam mosaic virus monoclonal antibodies in mice peritoneum. African Journal of Biotechnology 10(54):1117811181 Thomson Reuters indexed 9. Eni, A. O., Hughes, J. d’A., Asiedu, R. and Rey, M. E. C. (2010). Survey of the incidence and distribution of viruses infecting yam (Dioscorea spp.) in Ghana and Togo. Annals of Applied Biology 156(2):243-251. Thomson Reuters indexed 10. Eni, A. O., Hughes, J. d’A. and Rey, M. E. C. (2010). Production of polyclonal antibody against an isolate of yam-infecting badnavirus from Nigeria. Australian Journal of Basic and Applied Sciences 6(5): 4158-416. Thomson Reuters Journal 11. Eni, A. O., Hughes, J. d’A. and Rey, M. E. C. (2010). Production of polyclonal antibodies against a yam isolate of Cucumber mosaic virus (CMV). Research Journal of Agriculture and Biological Sciences 6(5): 607-612. 5 12. Eni, A. O., Hughes, J. d’A., Asiedu, R. and Rey, M. E. C. (2008). Sequence diversity among badnavirus isolates infecting yam (Dioscorea spp.) in Ghana, Togo, Benin and Nigeria. Archives of Virology 153(12):2263-2272. Thomson Reuters indexed 13. Eni, A. O., Hughes, J. d’A. and Rey, M. E. C. (2008). Survey of the incidence and distribution of five viruses infecting yam in the major yam producing zones in Benin. Annals of Applied Biology 153(2):223-232. Thomson Reuters indexed 14. Eni, A. O., Kumar, P. L., Asiedu, R., Alabi, O. J., Naidu, R. A., Hughes, J. d’A. and Rey, M. E. C. (2008). First Report of Cucumber mosaic virus in yams (Dioscorea spp.) in Ghana, Togo, and Republic of Benin in West Africa. Plant Disease 92(5):833. Thomson Reuters indexed Food safety and others 15. Oranusi, S., Omagbemi, F. and Eni, A. O. (2011). Microbiological safety evaluation of snacks sold in fast food shops in Ota, Ogun state, Nigeria. International Journal of Agricultural and Food Science 1(4):75-79 16. Eni A. O., Oluwawemitan, I. A. and Oranusi U. S. (2010). Microbial quality of fruits and vegetables sold in Songo Ota, Nigeria. African Journal of Food Sciences 4(5):291-296. 17. Chinedu, S. N., Eni A. O., Adebayo I. A. and J. A. Ayangbemi (2010). Assessment of growth and cellulose production of wild-type microfungi isolated from Ota, Nigeria. Asian Journal of Plant Sciences 9(3):118-125. Thomson Reuters Journal CONFERENCE CONTRIBUTIONS 18. Eni A. O., R. Mundembe and MEC Rey (2013). South African Cassava mosaic virus AC1/AC4 mismatched hairpin transgene reduces SACMV symptoms in transgenic cassava. 18th Biennial Conference of the South African Society of Microbiology (SASM), 24-27 November, 2013, Bela Bela, South Africa. 19. Eni, A. O., J. d’A. Hughes and M. E. C Rey (2013). Status of viruses infecting yam in West Africa. 12th International Plant Virus Epidemiology Symposium, 28th January - 1st February 2013, Arusha, Tanzania. 20. Eni, A. O., J. d’A. Hughes, R. Asiedu and M. E. C Rey (2012). Evaluation of Yam Mosaic Virus (YMV) detection methods. 12th Annual Scientific Conference of the Nigerian Society for Experimental Biology (NISEB) 14th-17th March 2012. University of Benin, Nigeria 21. Eni, A. O., J. d’A. Hughes and M. E. C Rey (2011). Yam virus diseases: A threat to a food security crop in West Africa. Joint meeting of the American Phytopathological Society and the International Association for the Plant Protection Sciences. 6th-10th August, 2011 Honolulu, Hawaii, USA. 22. Eni, A. O., Dike, I. P., Omonhinmin A. C. and Fasasi D. K. (2011).Molecular detection of two Begomoviruses infecting cassava in Southern Nigeria. In: Proc. 11th Annual Scientific Conference of the Nigerian Society for Experimental Biology (NISEB) 28th -31st March 2011. University of Lagos, Nigeria 23. Eni A. O., J. d’A Hughes and M. E. C. Rey (2010) Production of polyclonal antibodies for the detection of a yam isolate of Cucumber mosaic virus (CMV). In: Proc. 45th Annual Conference of the Science Association of Nigeria (SAN) 23rd – 27th May 2010. Niger Delta University Wilberforce Island Bayelsa State, Nigeria. 6 24. Eni A. O., Fasasi D. K., Oyelade O. O. and Duru A. C. (2010) Molecular detection of two groups of viruses infecting cultivated crops and associated weeds in Anambra, Delta, Edo, and Ondo states in Nigeria. In: Proc. 2nd International Biotechnology Symposium, 23th – 26th March 2010, Covenant University Ota, Nigeria, pp 44-45. 25. Eni, A. O., J. d’A. Hughes, R. Asiedu and M. E. C Rey (2009). Production of ascetic fluid (monoclonal antibodies) for detection of yam mosaic virus. In: Proc. 1st International Biotechnology Symposium, 28th – 30th April 2009, Covenant University Ota, Nigeria, pp. 9. 26. Eni, A. O, Dike, I. P, Omonhinmin A. C, Chinedu, S. N, Ogunlana, O. O, Fatumo, S. A, Adebiyi and E. F, Adebayo A. (2009). In-silico development of PCR primers for detection of endogenous pararetroviruses & episomal viral sequences. 17th Annual international conference on the Intelligent Systems for Molecular Biology (ISMB) and 8th European Conference on Computational Biology (ECCB) 27th June-2nd July 2009 Stockholm, Sweden 2009 http://www.iscb.org/ismbeccb2009/poster_list.php?cat=D 27. Dike, I. P, A. C. Omonhinmin, E. F. Adebiyi, A. O. Eni, S. N. Chinedu, F. Ibikunle, I. Ekwejobi and O.O. Ogunlana (2009) In-silico analysis of prospective African strains of algae for bio-diesel production. 17th Annual international conference on the Intelligent Systems for Molecular Biology (ISMB) and 8th European Conference on Computational Biology (ECCB) 27th June-2nd July 2009 Stockholm, Sweden 2009 http://www.iscb.org/ismbeccb2009/poster_list.php?cat=J 28. Ayangbemi, J. A., I. A. Adeniyi, A. O. Eni and S. N. Chinedu. (2009) Optimization of cellulolytic microfungi for bioremediation and waste management. In: Proc. 1st International Biotechnology Symposium, 28th – 30th April 2009, Covenant University Ota, Nigeria, pp. 36 29. Omonhinmin, A. C., I. P. Dike, P. C. Ekenna, E. F. Adebiyi, S. N. Chinedu, O. O. Omotosho and A.O. Eni. (2009). In-silico prediction and analyses of anti-fungal PR-5 thaumatin-like proteins in Thaumatococcus daniellii. In: Proc. 1st International Biotechnology Symposium, 28th – 30th April 2009, Covenant University Ota, Nigeria, pp. 28 30. Eni, A. O., J. d’A. Hughes, R. Asiedu, R. Bandyopadhyay, P. Lava Kumar and M. E. C. Rey (2007). Incidence and distribution of virus and virus-like diseases on yams (Dioscorea sp.) in the Republic of Benin. In: Proc. 10th International Plant Virus Epidemiology Symposium, 15th - 19th October 2007, ICRIST, Hyderadad, India, pp. 123. 31. Kumar, P. L., J. Mgbechi-Ezeri, A. O. Eni, S. Akinbade, R. Asiedu, A. Dixon, C. Fatokun and R. Bandyopadhyay, (2007). The challenges and potential options for controlling virus diseases of legumes and tuber crops in West Africa, In: Proc. 10th International Plant Virus Epidemiology Symposium, 15th – 19th October 2007, ICRIST, Hyderabad, India, pp. 60. 32. Hughes, J. d’A., A. O. Eni, and M.E.C. Rey (2004). A rapid field immuno-chromatographic assay for certification of yam planting materials. In: Proc. Ninth triennial symposium of the International Society for Tropical Root Crops - Africa Branch, 31st October–5th November 2004, Mombasa, Kenya. OTHER CONFERENCES/WORKSHOPS/CERTIFICATIONS Selected participant at the West African Virus Epidemiology (WAVE) for root and tuber crops convening organized by the Bill and Melinda Gates Foundation. 9th – 14th March 2014, Seattle, USA. 7 Selected participant at the West African Virus Epidemiology (WAVE) for root and tuber crops convening organized by the Bill and Melinda Gates Foundation. 17th – 20th March 2014, Rothamsted Research/Cambridge University/Natural Resources Institute, United Kingdom. Selected participant Leadership/Management Skills Course for Scientists organized by the Bill & Melinda Gates Foundation/hfp consulting. 28th -30th November, 2013, Entebbe, Uganda, Research Scholarship and Academic Administration Symposium Jointly organized by Covenant University and University of Florida. 16th November, 2013, Covenant University Ota, Nigeria. Selected participant at the Agricultural Research Connection workshop organized by BecAILRI Hub and the Bill & Melinda Gates Foundation. 28th July – 2nd August, 2013. Nairobi/Naivasha, Kenya. Selected participant at the Bill & Melinda Gates Foundation convening on West Africa virus diagnostics for cassava, sweet potato and yam. 27th – 28th June, 2013, Accra, Ghana. 7th Regional Plant Biotechnology Forum. 9th April 2013, University of Pretoria, South Africa. 6th Regional Plant Biotechnology Forum. 12th October, 2012, University of Johannesburg, South Africa. African Regional Forum on Best Practices in fostering food Security in Africa. 3rd March 2012. Olusegun Obasanjo Presidential Library Complex, Oke Mosan, Abeokuta, First African Virtual Conference on Bioinformatics. 19th – 20th February 2009, Covenant University, Ota, Nigeria. Conference on Plant Virology in Sub-Saharan Africa, 4th – 8th June 2001, International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria. Molecular Biology and Biotechnology workshop, Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research (NIMR), Yaba, Nigeria (2009). 2nd International workshop on Pattern Discovery in Biology, Department of Computer Sciences, Covenant University Ota, Ogun State, Nigeria (2009). Statistics and Statistical Computing with SAS, International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria (2008). Introduction to procurement, International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria (2007). Introduction to Bioinformatics, West African Bioinformatics Institute (WABRI), University of Ilorin, Nigeria (2006). ‘How to write and publish a scientific paper’, International Institute of Tropical Agriculture (IITA) Ibadan in collaboration with Prof. John Leslie, Kansas State University, USA (2003). Scanning Electron Microscopy and Application of Separation Techniques in Molecular Biology, The Central Science Laboratory, Obafemi Awolowo University Ile-Ife, Nigeria (2002) Use of Oracle application for on-line transactions, International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria (2001) Certificate d’Etudes de Francais Pratique 1er Degre, Alliance Française, Paris, France (2001). 8 Use of the Scanning and Transmission Electron Microscopes, International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria (2000) Molecular diagnostic techniques, International Institute of Tropical Agriculture (IITA) Ibadan in collaboration with Dr. Paul Keese Molecular Biologist CAMBIA, Australia (2000) Certificate in Spreed-sheet using Microsoft Excel 97 (Distinction), Computer Unit, International Institute of Tropical Agriculture (IITA), Nigeria (2000). Management supervisory course, International Institute of Tropical Agriculture (IITA) Ibadan, Nigeria (1999) Certificate for completion of debutante 1 course in French, Community Resources Center, International Institute of Tropical Agriculture (IITA), Nigeria (1999). MEMBERSHIP OF PROFESSIONAL BODIES/RESEARCH GROUPS 1. 2. 3. 4. 5. 6. Nigerian Society for Experimental Biology (NISEB) Science Association of Nigeria (SAN) African Society for Bioinformatics and Computational Biology (ASBCB) American Society for Microbiology (ASM) International Society for Computational Biology (ISCB) Covenant University Bioinformatics and Biotechnology Research Cluster EDITORIAL ENGAGEMENT 1. Reviewer for Plant Disease a journal of the American Phytopathological Society 2. Reviewer for the African Journal of Agricultural Research APPOINTMENTS (OUTSIDE THE UNIVERSITY) 1. Public Relations Officer, Nigerian Society for Experimental Biology (NISEB) 2012-2014 2. Member, Popularization of Science Committee (POPSCO), Science Association of Nigeria (SAN) 2010-2011. 3. Judge, Project Forte, a youth empowerment program of the Youth Church of The Redeemed Evangelical Mission (TREM) (2008, 2009 and 2011 editions). 4. Chairperson, Zonal Safety Sub-Committee, International Institute of Tropical Agriculture (IITA) Ibadan (2003-2008). The zone comprised of over 70 staff. 5. Executive member, Faculty of Biological Sciences Electoral Commission, University of Nigeria, Nsukka, 1997. 6. Financial secretary, National Association of Botany Students (NABOS), University of Nigeria, Nsukka chapter, 1995-1996. SERVICES WITHIN COVENANT UNIVERSITY 1. Member, Covenant University Ceremonies Committee, 2013/2014 Academic Session. 2. Chair, Decoration, Awards and Gifts Sub-Committee, Covenant University Ceremonies Committee, 2013/2014 Academic Session. 9 3. Member, Protocol and Welfare, Sub-Committee, Covenant University Ceremonies Committee, 2013/2014 Academic Session. 4. Member, Timetable and Examination Committee, 2013/2014 Academic Session. 5. Member, Local Organizing Committee, 26th Conference of the Association of Vice Chancellors of Nigerian Universities hosted by Covenant University in June 2011. 6. Chair, Fund raising/Exhibition Sub-Committee, 26th Conference of the Association of Vice Chancellors of Nigerian Universities hosted by Covenant University in June 2011. Successfully raised 12.8 million naira for the hosting of the conference. 7. Member, Transport Sub-Committee, Convocation Planning Committee, 2010 8. Member, Committee on Research Training Workshop, 2009. 9. Member, Protocol Committee, 2nd International Workshop on Pattern Discovery in Biology (2009) Hosted by the Department of Computer and Information Sciences. SERVICES WITHIN THE DEPARTMENT 1. Departmental Examination Officer, 2013/2014 Academic Session 2. Secretary, Organizing Committee, 3rd IBS, 2011. 3. Member, Organizing Committee, 2nd IBS, 2010. 4. Chair, Sponsorship Sub-Committee 2nd IBS, 2010. 5. Member, Welfare Sub-Committee, 2nd IBS, 2010. 6. Member, Organizing Committee, 1st IBS, 2009. 7. Chair, Welfare Sub-Committee, 1st IBS, 2009. 8. Member, Sponsorship Sub-Committee 1st IBS, 2009. 9. Academic level adviser, 2009/2010, 2010/2011, 2011/2012 Sessions. 10. Seminar Coordinator, Microbiology Unit, 2010/2011 11. Secretary, Departmental Website Update Committee, 2009/2010. 12. Member, Community Impact Committee, 2011/2012 13. Member, Research Committee, 2009/2010, 2010/2011 and 2011/2012. 14. Member, Laboratory Committee, 2009/2010, 2010/2011 and 2011/2012. 15. Member, Academic Advisory Committee, 2009/2010, 2010/2011 and 2011/2012. LANGUAGES Official: English (excellent written and verbal communication skill) French (intermediate level) Local: Ibo & Hausa (excellent verbal communication skill)