THE UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO
IN VIVO BIOHAZARD CONTAINMENT EVALUATION
The purpose of this analysis is to assist you, the Principal Investigator, the Institutional Biosafety Committee, and Laboratory
Animal Resources (LAR) in determining whether or not your project requires containment in the biohazard space and to
help LAR personnel care for infected animals in the safest manner possible. Please refer to the Institutional Biosafety
Manual, and the CDC and NIH "Biosafety in Microbiological and Biomedical Laboratories" for information concerning
biohazard levels and recommended containment. Please forward this form to the Environmental Health and Safety Office.
1.
Species of animals and approximate number to be housed at any given time:
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__________________________________________________________________________________________
2.
Will animals be infected with a biohazardous agent or rDNA?
3.
What agents will be used to infect the animals? _
_____________
__________________________________________________________________________________________
__________________________________________________________________________________________
4.
What routes will you use to infect these animals? Ip
Feed
Water bottle
Other
5.
What animal biosafety level (ABSL) is required?
6.
Risk containment analysis (factors influencing transmission and control of the agents)
YES
iv
Please specify:
ABSL 1
sc
ABSL 2
NO
id
_______
ABSL 3
gavage
ABSL 4



7.
Are infectious aerosols likely to be formed during experimentation?
YES
NO
Host range ( other animals, humans?):
_____________
Are infected animals likely to shed or excrete the agent(s)?
YES
NO
If YES, in what quantities and by what route?
_________
______________________________________________________________________________________
 Is unintentional animal-to-person or animal-to-animal transmission likely when infected animals are kept in
conventional housing?
YES
NO
If YES, what isolation and/or caging requirements would minimize or eliminate unintentional infection?
____________________________________
 Do animal handlers and technicians need special clothing or protective equipment other than gowns or gloves?
YES
NO
If YES, what is the recommended? _
_____________
 Is 10 minutes exposure of contaminated surfaces to Wescodyne (3 oz. Stock solution per gallon of water)
adequate for inactivating the agent(s) you use to infect the animals?
YES
NO
 Are certain people (e.g., pregnant women) more "at risk" (more likely to be infected and/or have a disease)
upon exposure to this agent?
YES
NO
 Is disposal of dead infected animals by double bagging, labeling with biohazard tag and incineration, adequate?
YES
NO
If NO, what is recommended? _
__________
 Do animal care or technical personnel need special health surveillance as a result of working with the infected
animals?
Y ES
NO
If YES, what is the recommended? _
____________
Principal investigators and laboratory supervisors working with biohazardous agents are primarily responsible for
control of these agents. Before work commences on animals, training must be given to technical and animal care
personnel concerning containment procedures and what to do in cases of injury or accidents. Documentation of
training should be kept on file. These standard operating procedures (SOPs) should be kept available to all
personnel. Training should be arranged through the Director of LAR at least 5 days prior to starting the
experimental work.
Department:
______
Office Phone #:
____________
Principal Investigator (Print)
Lab #:
____________________________________
Principal Investigator (signature)
Date:
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Source of Funding:
____
____
Revised May 6, 2003
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