2014 MRC성과발표회
Abstract Information
[
] 심포지엄 구연 발표
[ O ] 기초의과학 학문후속세대 발표
Category (연제와 관련있는 분야에 모두 표시해 주십시오)
[
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[
] Alternative medicine
[
] Anatomical methodology
[
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[
] Bioengineering and technology
[
] Biology of development
[
] Biomarkers for personalized medicine
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] Biomedical informatics
[
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[
] Cellular injuries, death, apoptosis, autophagy
[
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[
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[
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[ O ] Neuroscience and Neurobiology
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[
] Signal transduction
[
] Stem cells and regeneration
[
] Therapeutic approaches of Korean Medicine
[
] Vascular Biology and Medicine
[
] 기타 (
)
Insulin-like growth factor-1 is an important determinant of activity-induced increases
in the survival of graft neural stem cells after spinal cord injury
Dong Hoon Hwang
Department of Brain Science, Ajou University School of Medicine, Suwon, Republic of Korea.
We have previously demonstrated that neural stem cell (NSC) survival rapidly declined after
grafting and combined treadmill locomotor training (TMT) increased the survival of grafted
NSCs. We found that insulin-like growth factor-1 (IGF-1) levels in CSF were increased by
TMT, suggesting that IGF-1 induced by TMT play a prosurvival function for grafted NSC. The
current study tested the hypothesis that IGF-1 is a key factor to regulate the survival of
NSCs following transplantation into the injured spinal cord. Intrathecal delivery of neutralizing
antibodies against IGF-1, but not either BDNF or NT-3, abolished the enhanced survival of
NSC grafts by TMT. IGF-1 neutralization also reduced the number of ERK positive grafted
NSCs. We also examined if genetic deletion of IGF-1 receptor (IGF-1R) may render grafted
NSCs unresponsive to activity-based training. NSCs obtained from IGF-1R (+/-) were more
susceptible to the cellular stresses induced by reactive oxygen or nitrogen species that those
from IGF-1 (+/+) mice. We also compared the graft survival between NSCs from IGF-1R (+/) and (+/+) mice transplanted in wild type mice with combined TMT. The TMT-induced
survival gain was substantially abrogated when IGF-1R (-/+) NSCs were transplanted.
Interestingly, most grafted NSCs derived from IGF-1R (+/-) mice were positioned around the
injection site, whereas numerous grafted NSCs with two copies of IGF-1R showed extensive
migration and frequently integrated with residual spinal cord tissue. These results indicate
that the IGF-1 is a central regulator of the survival of graft neural stem cells after spinal cord
injury.
Keywords
Spinal cord injury, Treadmill, Neural Stem cell, Insulin Like Growth Factor 1
2014 MRC 성과발표회
Name : Dong Hoon Hwang
Affiliation : Department of Brain Science,
Ajou University School of Medicine
Position : Research assistent professor
E-mail : drhdh@ajou.ac.kr
Field of Expertise:
Stem cell and Spinal cord injury
Education :
Graduate school of Ajou University, Neuroscience Graduate Program, PhD, August 2009
Professional Experience : (less than 5 experiences)
2004. 9. 1～ 2005. 2. 28
Research Technician
2006. 3. 1～ 2009. 8. 31
Graduate Assistant
School of Medicine, Ajou University
2009.10. 1～ 2010. 8. 31
Research Fellow
School of Medicine, Ajou University
2010. 9. 1～ Present
Research assistent professor
University of British Columbia (Canada)
School of Medicine, Ajou University
Selected Publications : (less than 5 papers)
1. Survival of Neural Stem Cell Grafts in the Lesioned Spinal Cord Is Enhanced by a
Combination of Treadmill Locomotor Training via Insulin-Like Growth Factor-1 Signaling. J
Neurosci. 2014 Sep 17;34(38):12788-800.
2. Molecular and cellular changes in the lumbar spinal cord following thoracic injury: regulation
by treadmill locomotor training. PLoS One. 2014 Feb 10;9(2):e88215.
3. Contribution of macrophages to enhanced regenerative capacity of dorsal root ganglia
sensory neurons by conditioning injury. J Neurosci. 2013 Sep 18;33(38):15095-108.
4. Gene transfer mediated by stem cell grafts to treat CNS injury. Expert Opin Biol Ther. 2011
Dec;11(12):1599-610. Review
5. Combination of multifaceted strategies to maximize the therapeutic benefits of neural stem
cell transplantation for spinal cord repair. Cell Transplant. 2011;20(9):1361-79.