BB 9/15/05 Updated 6/15/07 JEB
Borate Buffer (4º) *
     4.76 g Boric Acid (Cat # B-1934) 2.54 g Borax (Cat # B-0127) 1000 mL of ddH 2 O pH to 8.4 with NaOH Sterile Filter
Plating Media (4º)
     
Neurobasal (4º)
       (Cat # 21103) Sterile Filter Aliquot 0.5 mL
□
6 mL sterile H 2 O
□
40 mL F 12 (Cat # N8641) 40 mL FBS (Cat # sh30070.03) 420 mL Dulbecco Modified Eagle Medium (DMEM) W/O L-Glutamine (Cat # 11960) 1 mL of PS (Cat # 17-602E) 200 uL of 200 mM L-Glutamine (Cat # G7513)
D 2 C (4º) □
    10 mL FBS (Cat # sh30070.03) 1 mL PS (Cat # 17-602E) 12.5 mL of 1M Hepes (Cat # H0887) 471.5 mL Dulbecco Modified Eagle Medium (DMEM) W/O L-Glutamine (Cat # 11960) 6.25 mL of 200 mM L-Glutamine (Cat # G7513) 1 bottle (500 mL) Neurobasal Media 1 bottle (10 mL) 50X B27(Cat # 17504-044) P/S (Cat # 17-602E)
Stock Ara-C (500X) (-20º) *
100 mg Ara-C (Cat # C-6645)
Working Ara-C (4º) *
 20µL Stock  10 mL sterile T.C. water
10% Formaldehyde (RT or 4°) *
  5 mL of 37% Stock Solution 45 mL of PBS
Glucose Free Balanced Salt Solution *
       5.26 g NaCl (Cat # S9625-5KG) 0.125 g KCl (Cat # P-9541) 66.6 mg CaCl 2 (Cat # C-4901) 1.43 g Hepes (Cat # H-4034) 600 mL ddH 2 O Sterile Filter pH 7.3
PLO Δ
 1 mg PLO : 2 mL Borate Buffer (Cat # P3655)   Sterile filter Make up fresh each time it is used
HBSS (4º) *
       8.4 g NaCl (Cat # S9625-5KG) 224 mg KCl (Cat # P-9541) 2.38 g Hepes (Cat # H-4034) 1.0 g Glucose (Cat # G-5250) 1000 mL Milli Q pH to 7.3 with NaOH Sterile Filter
Roccal II (10%) (RT) *
 10% Benzalkonium Chloride (Cat # 234427 Sigma)    1.25% Ethyl Alcohol Dissolve in alcohol with stirring Add 88.75% Milli Q water
MEM/BSA/Hepes □
    487.5 mL of clear MEM (Cat # 51200) 12.5 mL of 1 M Hepes (Cat # H0887) 50 mg of BSA (Cat # A7888) Sterile Filter
* =good for months, □
=good for ~1 week, Δ = make fresh Note: We are no longer heat inactivating our Fetal Bovine Serum