Eric S. Kraft
Education
Fordham University
- MS in Computer Science received in May of 2001 with an emphasis in
programming
- Overall GPA: 3.9
University of North Carolina at Chapel Hill
- Received a Thesis Masters of Science in Analytical Chemistry in
May of 1996 with an emphasis on biochemistry
- Overall GPA: 3.8
State University of New York at Binghamton
- Received a Bachelor of Science in Chemistry in May of 1994
- Overall GPA: 3.5
Chemistry GPA: 3.5
Experience & Research
April 2006 –January 2008
Principle Scientist, Chemical Development
GlaxoSmithKline
- Elucidated structures of unknown stability and forced degradation
compounds using LTQ iontrap, Thermo Electron TSQ triple quadrupole,
Waters QTOF, and Thermo Electron Orbitrap mass spectrometer.
- Determined structures of unknown synthetic impurities in API and precursor
compounds using LC-MS/MS.
- Quantified analytes by LC-MS/MS using ThermoElectron TSQ triple
quadrupole instrument.
- Re-developed software for molecular ion assignment from LC-MS spectral
data for walk-up LC-MS system. The system is used by synthetic chemists
to analyze reaction mixtures and determine main components by analysis of
mass spectral data. A customized report for synthetic chemists is generated.
The software package is programmed in Visual Basic and ChemStation
Macro language.
- Measured accurate mass of unknown compounds using TOF, Thermo
Electron TSQ Accurate Mass Quadrupole, and Orbitrap mass spectrometers.
- Developed in house methodology for application of Atmospheric Pressure
Photo Ionization source (APPI) on TSQ and LTQ mass spectrometers.
- Documented results and reported information to development teams.
- Developed Ion-Exchange-MS methodology for analysis and LC-MS
quantification of non-chromophoric poorly retained synthetic precursors.
- Synthesized chemical markers for degradation and synthetic impurities.
- Made presentations discussing work as needed.
July 2004 – April 2006
Scientist, Nonclinical Drug Safety
Hoffman La Roche
- Developed quantitative LC-MS/MS methods for toxicology compounds and
metabolites on Sciex 4000, Micromass Ultima and Micromass Premier
instruments. Studies and analysis were performed under GLP regulations.
- Developed methods and analyzed via LC-MS/MS a variety of tissue types
for discovery DMPK studies.
- Automated toxicology sample preparation using Biomek FX robotics.
Biomek FX interacts with Watson LIMS database through VB scripting to
automatically determine toxicology sample dilutions, plate locations and
standard/QC preparation. The software has flexible user options for protein
precipitation, liquid/liquid extraction, and solid phase extraction.
- Trained coworkers on the use of robotics and Sciex mass spectrometers
through presentations, documentation and interactive training sessions.
- Acted as bioanalytical coordinator for development projects.
-
Developed methods for the quantitation of therapeutic peptides in plasma
using LC-MS/MS.
November 2001 – July 2004 Scientist, Bioanalytical Chemistry for Drug Discovery and Clinical Department
Biogen Corporation
- Determined structure of unknown in-vivo/vitro metabolites for drug
discovery compounds.
- Used LC-MS/MS to determine pharmacokinetic and pharmacodynamic
profiles for early discovery compounds in small molecule program.
- Developed GLP LC-MS/MS assays for analysis of preclinical and clinical
urine and plasma samples.
- Developed general LC methods for rapid chromatographic method
development for novel discovery compounds.
- Initiated in-vitro metabolism screen methodology to determine metabolic
stability of discovery compounds.
- Automated pharmacokinetic sample preparation using Packard, Tecan, and
TomTec liquid handling robots. Reduced sample preparation time by 90%
using robotic methodology and post dose pooling.
- Automated in-vitro rat microsomal screen for discovery compounds using
Packard robotic systems.
- Analyzed pharmacokinetic data using Win-nonlin software.
- Used MALDI-TOF instrumentation for examination of peptides and
proteins for initial stage of proteomics project.
- Automated SELDI plasma sample preparation with 96 well ion exchange
fractionation and SELDI spotting on Packard robotics for proteomics
project.
- Developed protein separations for multidimensional chromatography using
ion exchange, reverse phase, and SEC methods.
- Familiar with operation and maintenance of Micromass, ThermoElectron
and Sciex mass spectrometer instrumentation and various HPLC equipment.
Supervised and trained temporary personnel.
June 1997 – November 2001 Scientist, Analytical Research & Development Division
Purdue Frederick Research Center
- Developed and applied LC – MS/MS methods to analyze compounds of
interest and elucidate structures of degradation products and impurities.
Familiar with ESI, APCI, EI ionization sources and TOF, FTMS, and triple
quadrupole type mass analyzers.
- Used LC-MS/MS to examine and optimize synthetic routes used by organic
chemists in pilot plant and medicinal chemistry groups.
- Involved in the development of instrumentation and methods used in the
construction of probes to measure drug concentrations.
- Purified compounds of interest to the manufacturing facility by
preparative scale chromatography.
- Knowledgeable in a wide variety of pharmaceutical processes, equipment,
testing methods, and techniques.
- Performed Karl Fisher, dissolution, content uniformity, HPLC assay, related
substance, and UV assays.
- Performed experimental design for evaluation of analytical methodology.
- Tested a variety of dosage forms using many different analytical techniques.
- Developed and validated HPLC methods used in FDA submissions.
- Calibrated, repaired, and maintained mass spec. and HPLC equipment.
GMP trained.
May 1995 – May 1996
Research Assistantship, Analytical Chemistry
UNC Chapel Hill Department of Chemistry, Dr. Jorgenson
- Developed Capillary Zone Electrophoresis at 100,000 to 150,000 volts.
Chemistry program had a heavy emphasis on biochemistry, with classes
taken in general biochemistry, nucleic acids/genes, and proteins.
Spring 1993 – Spring 1994
Research Assistant, Bioanalytical Chemistry
State University of New York at Binghamton, Dr. Hartwick
- Worked on Micellar Electrokinetic Capillary Chromatography project
involving chiral phospholipid pseudophases and cyclodextrins to obtain
enantiomeric separations of pharmaceutical compounds.
Summers of 1992 & 1993
Winter 1993
Research Assistant, Inorganic Chemistry
Hunter College, New York, NY, Dr. Francesconi
- Synthesized and characterized [K13(SiW11O39)2M]*30 H2O where M is a
metal in the lanthanide series. In conjunction with the NIH the compound
was investigated as an AIDS treatment. A paper is published on this
research.
Chemistry and
Computer Skills
-
-
Teaching
Experience
-
Honors &
Activities
-
Mass Spectrometry, LC-MS/MS
High Performance Liquid Chromatography, Gas Chromatography
CZE and Micro-Column Liquid Chromatography
GLP and GMP trained.
Method development and validation
Robotic automation
Infrared and Ultraviolet-Visible Spectroscopy
C++ , Visual C++, HTML, VB scripting, database and Java programming
Electrochemistry
Electronics
Graphics, Spreadsheets, and Word Processing programs
Teaching Assistant for the Analytical Chemistry Lab and Advanced
Analytical Chemistry Lab , UNC-CH Dept. of Chemistry, 1994-1996
Teaching Assistant for the Graduate Level Electronics Laboratory, UNCCH Dept. of Chemistry during the fall semester of 1995
Received Silver Award at GSK for developing Walk-Up LC-MS software
Received 3 Recognition Awards at Hoffman La Roche for bioanalytical
methodology and robotics
Dean’s List for four consecutive semesters
Phillips Van Heusan Undergraduate Scholarship
References Available Upon Request