Identification of proteins associated with ion homeostasis and salt
tolerance in barley
Dezhi Wu1, Qiufang Shen1a, Long Qiu2, Yong Han1, Linzheng Ye1, Zahra Jabeen1, Qingyao Shu3,
Guoping Zhang1,*
1
Department of Agronomy, Key Laboratory of Crop Germplasm Resource of Zhejiang Province,
Zhejiang University, Hangzhou 310058, China
2Life
Science and Technology Center, China Seed Group Co., Ltd., Wuhan 430070, China
3Institute
a
of Nuclear Agricultural Science, Zhejiang University, Hangzhou 310058, China
Equal contribution to this work
*Corresponding
author:
Prof. Guoping Zhang
Fax: +86 571 8898 2115;
E-mail address: zhanggp@zju.edu.cn
Supplemental Figures and Tables:
Fig. S1. Two-dimensional (2-D) gels of the differentially expressed protein spots in the
roots and leaves of barley genotypes CM72 and XZ16.
Fig. S2. Gel spots of the proteins up-expressed in roots and leaves of XZ16.
Table S1. The normalized spot volume of protein spots in roots of barley genotypes
CM72 and XZ16 under normal and salinity condition.
Table S2. The normalized spot volume of protein spots in leaves of barley genotypes
CM72 and XZ16 under normal and salinity condition.
Figure S1. Two-dimensional (2-D) gels of the differentially expressed protein spots
in the roots and leaves of barley genotypes CM72 and XZ16. The numbers in black
show the differentially expressed protein spots in XZ16 and the numbers in green
indicate spots for CM72. D and U the numbers indicates down- or up- regulated
protein spots, respectively.
Figure S2. Gel spots of the proteins up-expressed in the roots (a) and leaves (b) of
XZ16. (a), BU1: succinyl-CoA ligase; BU2: H+-ATPase (subunit alpha); BU3:
Monodehydroascorbate reductase; BU4: Putative class III peroxidase; BU5: Putative
class III peroxidase; BU6: Putative class III peroxidase; BU9: Pathogenesis related
protein 10. (b), BU1: Harpin binding protein 1; BU2: an uncharacterized protein. (c),
Trans-membrane (TM) region of class III peroxidase (BU4). Posterior probabilities of
sequence were tested using TMHMM Server v. 2.0 and the helical wheel diagram of
sequence was predicted using SOSUI software.
Table S1. Normalized spot volume of protein spots in roots of barley genotypes
CM72 and XZ16 under normal and salinity condition.
Note: The normalized spot volume of each spot was based on six gels (3 biological and 2
Normalized spot volume
CM72-Root
Spot
S.E.
XZ16-Root
CM72-Root
XZ16-Root
CK
Salt
Sig.
CK
Salt
Sig.
CK
Salt
CK
Salt
AD9
0.034
0.008
HS
0.026
0.016
S
0.004
0.002
0.007
0.003
AD 10
0.022
0.013
HS
0.017
0.013
NS
0.002
0.002
0.002
0.004
AD 11
0.048
0.019
HS
0.057
0.039
S
0.004
0.003
0.005
0.004
AD 13
0.064
0.014
HS
0.063
0.000
HS
0.006
0.002
0.012
0.000
AD 14
0.199
0.064
HS
0.154
0.125
NS
0.038
0.004
0.028
0.008
AD 15
0.142
0.036
HS
0.106
0.054
S
0.026
0.007
0.020
0.007
AU 1
0.013
0.015
NS
0.015
0.014
NS
0.003
0.002
0.004
0.004
AU 2
0.024
0.058
HS
0.022
0.064
S
0.006
0.006
0.003
0.009
AU 4
0.010
0.003
S
0.000
0.009
HS
0.002
0.001
0.000
0.002
AU 5
0.051
0.152
HS
0.022
0.072
HS
0.010
0.013
0.004
0.013
AU 8
0.064
0.100
S
0.050
0.048
NS
0.017
0.008
0.011
0.011
BD1
0.163
0.074
HS
0.165
0.060
S
0.027
0.012
0.028
0.011
BD 2
0.026
0.000
HS
0.070
0.067
NS
0.004
0.000
0.012
0.012
BU1
0.080
0.000
HS
0.050
0.078
S
0.013
0.000
0.008
0.014
BU 2
0.024
0.058
HS
0.022
0.064
S
0.006
0.006
0.003
0.009
BU3
0.010
0.020
S
0.015
0.028
HS
0.002
0.005
0.004
0.005
BU 4
0.054
0.029
S
0.019
0.058
HS
0.012
0.002
0.006
0.010
BU 5
0.008
0.002
HS
0.037
0.084
HS
0.002
0.000
0.009
0.006
BU 6
0.009
0.047
HS
0.011
0.028
HS
0.002
0.009
0.003
0.002
BU 9
0.091
0.098
NS
0.175
0.370
S
0.015
0.009
0.012
0.026
technical replicates for each treatment); A and B in figures respectively indicates CM72 and
XZ16. D and U marked the numbers indicates down- or up- regulated protein spots, respectively.
The significance of normalized spot volume of protein spots were analyzed using
SAS software (http://www.sas.com/software/sas9/) by a paired t-test. Statistical
difference at P <0.05 and P <0.01 is considered as significant and highly significant,
respectively. HS, S and NS indicate highly significant (P<0.01), significant (P<0.05) and no
significant difference, respectively.
Table S2. Normalized spot volume of protein spots in leaves of barley genotypes
CM72 and XZ16 under normal and salinity condition.
Normalized spot volume
Spot
CM72-Leaf
S.E.
XZ16-Leaf
CM72-Leaf
XZ16-Leaf
CK
Salt
Sig.
CK
Salt
Sig.
CK
Salt
CK
Salt
AD1
0.056
0.018
HS
0.056
0.037
NS
0.002
0.003
0.005
0.007
AD2
0.024
0.000
HS
0.035
0.017
HS
0.002
0.000
0.002
0.004
AD3
0.047
0.000
HS
0.035
0.044
NS
0.002
0.000
0.003
0.010
AD5
0.036
0.026
HS
0.038
0.019
S
0.006
0.004
0.005
0.003
AD6
0.055
0.000
HS
0.053
0.071
NS
0.004
0.000
0.006
0.016
AD7
0.029
0.000
HS
0.025
0.023
S
0.004
0.000
0.005
0.004
AD8
0.106
0.000
HS
0.100
0.082
NS
0.008
0.000
0.022
0.014
AU1
0.355
0.619
HS
0.518
0.450
NS
0.081
0.035
0.042
0.040
AU 2
0.037
0.142
HS
0.024
0.122
HS
0.012
0.017
0.004
0.016
AU 3
0.088
0.209
HS
0.106
0.205
S
0.006
0.016
0.008
0.033
AU 4
0.000
0.049
HS
0.000
0.000
NS
0.000
0.010
0.000
0.000
BD3
0.027
0.022
NS
0.027
0.000
HS
0.003
0.002
0.005
0.000
BD 4
0.031
0.041
S
0.028
0.021
HS
0.004
0.003
0.005
0.004
BD 5
0.069
0.000
HS
0.126
0.045
HS
0.022
0.000
0.010
0.007
BD 6
0.042
0.046
NS
0.058
0.000
HS
0.006
0.007
0.008
0.000
BD 7
0.046
0.000
HS
0.067
0.000
HS
0.011
0.000
0.003
0.000
BD 8
0.137
0.100
NS
0.075
0.039
HS
0.012
0.009
0.009
0.007
BD 9
0.071
0.047
HS
0.076
0.050
S
0.007
0.007
0.009
0.011
BD 11
0.070
0.055
S
0.096
0.083
HS
0.010
0.012
0.011
0.014
BU1
0.035
0.084
HS
0.040
0.098
HS
0.003
0.014
0.002
0.010
BU2
0.037
0.142
HS
0.024
0.122
HS
0.012
0.017
0.004
0.016
See Table S1 for more details.