Mucosal surfaces, reproductive health and
HIV in prevention research Workshop
2015
AGENDA
Tuesday 20 January 2015
11:00 – 11:30
Arrival at Cape Town
International Airport
11:30 -12:00
Transport to the Hilton Hotel
A light lunch will be provided in
the hotel lobby
Registration in Hilton Hotel
lobby
Transportation to Opening
Cocktail Function
Mucosal Surfaces Workshop
Opening: British High
Commission, Bishopscourt
16:30 - 17:30
17:45 – 18:30
18:30 – 20:30
Local delegates to be met Jean Mari Kriek
International delegates to be met by
Kathryn Norman
Welcome: British High Commission
representative
Opening Keynote (45 minutes)
Robin Shattock, Imperial College London,
UK
It is all about the mucosae
Closing and thanks
NRF and British Council representative
welcomes
Wednesday 21 January 2015
08:00 – 09:00
09:00 – 11:00
Transport to HVTN CHIL lab
Session 1:
Topic: The HIV prevention
landscape and converging
epidemics
Chair: Robin Shattock
Registration of UCT delegates
Linda-Gail Bekker, Desmond Tutu HIV
Foundation and University of Cape Town,
SA
Adolescence: vulnerabilities and
opportunities
30 mins talks + 10 mins
questions
Janneke van de Wijgert, University of
Liverpool, UK
The role of the cervicovaginal microbiome
and proteome in STI and HIV transmission
11:00 - 11:30
Tea Break
Leigh Johnson, University of Cape Town, SA
Modeling the impact of STI management on
the HIV epidemic
Tea, coffee and snacks
11:30 – 13:00
Oral abstract Session:
Topic: Microbicides to
prevent HIV
Chair – Florian Hladik
15 min talks + 5 min questions
Natalia Olenjniczak, Imperial College
London, UK
Evaluation of anti-proteases as potential
candidate microbicides
Alexandre Kabamba, Council for Scientific
and Industrial Research, Pretoria, SA
HIV shows increased sensitivity to
Griffithsin derivitives
Julia Makinde, Imperial College London, UK
Targeting cellular drug transporters to
boost the activity of HIV microbicides
Leonard Damelin, National Institute of
Communicable Diseases (NICD),
Johannesburg, SA
Aliginate microbead-encapsulated silver
complexes for the selective delivery of
broad-spectrum, silver-based microbicides
13:00 – 14:00
14:00 – 16:15
Lunch
Session 2:
Topic: Mucosal sampling in
HIV prevention research
Chair – Erica Andersen-Nissen
15 min talks + 5 min questions
Phillip Bergin, IAVI, London, UK
The need for standardized mucosal
sampling in HIV vaccine trails
Julie Czartoski & Gabriela Diaz, Fred
Hutchinson Cancer Research Center, Seattle,
USA
Impact of different mucosal sampling
methods: the importance of close clinic/lab
crosstalk
Lenine Liebenberg, CAPRISA, Durban, SA
Mucosal sampling in the context of a clinical
trial: Does order matter?
Goli Haidari, St Mary’s Hospital, UK
Clinical Experience of mucosal sampling in a
phase 1 clinical trial: challenges and lessons
learnt
16:15-17:15
Afternoon tea and poster
session:
Oral posters
Chair – Heather Jaspan
5 min talks + 5 min questions
Poster session
Alethea Cope, Imperial College London, UK
Measuring Mucosal Responses in Early
Phase Trials – A Lab Perspective
Phillippe Selhorst, University of Cape
Town, SA
Role of viral replicative fitness in the
CAPRISA 004 microbicide trial
Julie Fox, Guys and St Thomas NHS Trust,
London, UK
Pre-exposure Prophylaxis Fails to Prevent
HIV-1 Infection or Reservoir Establishment
Hendrik Kloverpris, K-RITH, Durban, SA
Tissue resident Innate Lymphoid Cells in
HIV-1 infection
Jonathan Sumnar, King’s College London,
UK
The role of HIV-1 Vpu in regulation of the
immune response
Suzanne Venables, Imperial College
London, UK
A Phase I Clinical Trial with a Novel gp41
HIV Vaccine (EN41-FPA2) in Healthy
Female Volunteers
Joanne McCaffrey, Belfast, Queen's
University Belfast
A Novel Technology Platform for Mucosal
DNA Vaccine Delivery
Sarah Joseph, University College London,
UK
Bacterial Vaginosis and HIV: an analysis of
the MDP 301 trial
Anna Blakney, University of Washington,
USA
A Hydrogel Model for Pharmacokinetic
Evaluation of Triple-Antiretroviral
Electrospun Fibers as a Microbicide
Roslyn Ray, University of Cape Town, SA
The Contraceptive MPA, unlike NET,
Modulates Expression of Immune Function
Genes and HIV-1 Infection in Cervical Tissue
Explants and PBMCs
Alexis Bick, University of Cape Town, SA
The progestin-only contraceptive
medroxyprogesterone acetate, but not
norethisterone acetate, regulates gene
expression in endocervical epithelial cells,
through the glucocorticoid receptor
19:00 - 19:45
19:45 – 21:00
Transport from Hilton Hotel to
dinner
Dinner at Steenberg Wine
Estate, Constantia at BISTRO
1682
HVTN-Gates evening
Hosted by Erica Andersen-Nissen
Thursday 22 January 2015
08:00 – 09:00
09:00 – 11:00
Transport to CHIL lab
Session 3:
New insights into mucosal
immunology
Chair: Jo-Ann Passmore
30 mins talks + 10 mins
questions
11:00– 11:30
11:30– 13:00
Tea Break
Oral abstract Session:
Topic: Understanding HIV
infection
Tom Hope, Northwestern
Chicago, USA
Antibodies at Mucosal Sites
University,
Rupert Kaul, University of Toronto, Canada
Mucosal effects of HSV2 infection and the
impact of antiviral therapy
Florian Hladik, University of Washington,
Seattle, USA
Microbicides and mucosal immunology
Tea, coffee and snacks
Jane Rasaiyaah, University College London,
UK
Cloaking of lentiviral replication by CPSF6
and cyclophilins in human monocyte
derived macrophages
Chair – Clive Gray
15 min talks + 5 min questions
Julia Weinelt, Kings College London, UK
Evading the issue: Vpu versus Tetherin
Sengiziwe Sibeko, Oxford University, UK
Constitutive MIP-3a levels in five matrices
of the female genital tract
Maria Teresa Rodriguez-Plata, University
College London, UK
The infectious synapse formed between
mature dendritic cells and CD4+ T cells is
independent of the presence of the HIV-1
envelope glycoprotein
13:00– 14:00
14:00– 15:00
Lunch
Session 4:
Topic: Mucosal tissue models
to study HIV transmission
Chair – Alethea Cope
15 min talks + 5 min questions
Carolina Herrera, Imperial College London,
UK
Mucosal models to study HIV transmission
Rena Astronomo, Fred Hutchinson Cancer
Research Center, Seattle, USA
Vaginal explant models to study the role of
antibodies in HIV infection
Janet Hapgood, University of Cape Town,
SA
Hormonal Contraception and explant
models in South Africa
15:20– 17:20
Explant practical session 1:
Chair – Erica Andersen-Nissen
17:20 -18:20
Afternoon tea and poster
session:
Oral posters
Chair – Shaun Barnabas
5 min talks + 5 min questions
Poster session
Sinaye Ngcapu, CAPRISA, Durban, SA
HIV target cells and the epithelial barrier
in genital biopsies from women who
became HIV infected in CAPRISA 004
Cervical and colorectal explants
Lead by Carolina Herrera, Maria Lemos,
Jessica Prodger, Lamar Fleming, Rena
Astronomo and Anneta Naidoo
Vineet Joag, University of Toronto, Canada
Identification of preferential targets of HIV
infection in the cervix
Marcus Dorner, Imperial College London,
UK
Early events in HIV transmission using
humanised mice
Chanel Avenant, University of Cape Town,
SA
Targeting the Glucocorticoid Receptor with
Selective Modulators for Prevention against
HIV-1 Infection
Cheli Kambaran, CAPRISA, Durban, SA
Phenotype and Frequency of Genital STI and
Commensal Microbe-Specific Th17 Cell
Responses in Women
Ashley Singh, CAPRISA, Durban, SA
Anatomical distribution, phenotyping and
frequency of T and B cells isolated from
cervical tissue
Shameem Jaumdally, University of Cape
Town, SA
Impact of Partner HIV Status on Immune
Activation and Inflammation During Chronic
HIV
Smritee Dabee, University of Cape Town,
SA
Immune Activation and HIV Target Cells in
the Adolescent Female Genital Tract
Nyaradzo Chigorimbo-Tsikiwa, University
of Cape Town, SA
Measurement of tight junction protein
upregulation in foreskin explants by small
molecultes using proteomic approaches
Evening at leisure
Friday 23 January 2015
08:00 –09:00
09:00– 11:00
Transport to CHIL lab
Session 5:
Innate immunity, STIs and
HIV risk
Chair: Rupert Kaul
20 mins talks + 10 mins
questions
Suzanna Francis, London School of
Hygeine and Tropical Medicine, UK
Immune activation in the female genital
tract: Expression profiles of soluble
proteins in women at high risk for HIV
infection
Caroline Tiemessen, National Institute for
Communicable
Diseases
(NICD),
Johannesburg, SA
Chemokines, chemokine receptors and HIV
infection risk
Kenneth Rosenthal, McMaster University,
Canada
Innate mucosal immune profile & factors in
resistance to HIV infection
11:00– 11:30
11:30– 12:45
Tea Break
Oral abstract Session:
Topic: STIs and Biomarkers
for HIV risk
Chair – Suzanna Francis
12 min talks + 3 min questions
Adam Burgener, University of Manitoba,
Canada
The mucosal proteome and HIV infection
risk
Tea, coffee and snacks
Shaun Barnabas, University of Cape Town,
SA
Genital inflammation and STIs in adolescent
women
Sylvie Amu, Karolinska Institute, Sweden
Gene expression of chemokines and
chemokine
receptors
in
cervical
cytobrushes and foreskin samples in
relation to inflammation and STIs
Lindi Masson, University of Cape Town, SA
Inflammatory cytokine biomarkers to
identify women with asymptomatic
sexually transmitted infections and
bacterial vaginosis who are at high risk of
HIV infection
Lyle McKinnon, CAPRISA, Durban, SA
Mucosal proteome associated with HIV
acquisition in CAPRISA 004
12:45 - 13:30
13:30– 14:30
Lunch
Session 6:
Francesca Chiodi, Karolinska Institute,
Sweden
B cells, antibodies and HIV
prevention research
B cells in the genital tract and their
relevance for antibody response to HIV
vaccines
Chair: Carolina Herrera
25 mins talks + 5 mins
questions
14:30– 15:00
15:00– 16:00
Tea Break
Session 4:
Topic: Foreskin explant
models to study HIV
transmission
Chair – Lyle McKinnon
15 min talks + 5 min questions
16:00– 17:45
Explant practical session 2:
19:30
Chair – Maria Lemos
Faculty Dinner (optional)
Evening free for delegates
Desh Archary, CAPRISA, Durban, SA
HIV-specific antibody responses in
the genital tract and blood of women with
breakthrough HIV-infections following 1%
Tenofovir gel use in the CAPRISA 004 trial
Tea, coffee and snacks
Maria Lemos, Fred Hutchinson Cancer
Research Centre, Seattle, USA
Explant systems to characterize antibody
responses at the foreskin
Jessica Prodger, Johns Hopkins Bloomberg
School of Public Health, USA
Isolating functional T cells from foreskin
tissues
Cobus Olivier, University of Cape Town, SA
The tip of the iceberg – impact of
asymptomatic STI’s on immune cells in the
male foreskin
Foreskins
(Carolina Herrera, Jessica Prodger, Maria
Lemos, and Anneta Naidoo)
Saturday 24 January 2015
08:00 - 08:45
Transport to DTHF Youth
Centre, Masiphumele, tour of
DTHF Adolescent Clinical
Trial site
09:00 – 09:30
Linda-Gail Bekker, DTHF, UCT, SA
Welcome to the center
9:30 - 10:00
Megan Mrubata, DTHF, SA
Walk around DTHF youth center
10:00 – 10:20
Thola Bennie, DTHF, SA
The MACHO experience
10:20 – 10:40
Melissa Wallace, DTHF, SA
Ethical issues involved with enrolling
adolescents in clinical trials
10:40 – 11:00
Future fighters
11:00 - 12:00
Linda-Gail Bekker, DTHF, SA
Walk around Masi
12:30 - 14:30
14:30 - 15:30
Final Lunch – Café Roux,
Noordhoek
Transport to Cape Town
International Airport