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Principal Investigator/Program Director (Last, first, middle):
John E. Wiley, Ph.D.
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel in the order listed for Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
John E. Wiley
Director, Clinical Cytogenetics Laboratory
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
UNC-Greensboro
UNC-Greensboro
NC State University
UW-Madison
DEGREE
(if applicable)
BA
MA
PhD
Postdoc
YEAR(s)
1973
1976
1981
1982-1984
FIELD OF STUDY
Biology
Biology
Zoology/Genetics
Cytogenetics
NOTE: The Biographical Sketch may not exceed four pages. Items A and B may not exceed two of the four-page
limit.
A. Positions and Honors. List in chronological order previous positions, concluding with your present position. List any
honors. Include present membership on any Federal Government public advisory committee.
Assistant Professor, Department of Pediatrics, Division of Medical Genetics, 1984-1991
Director, Clinical Cytogenetics Laboratory, ECU School of Medicine, Greenville, NC, 1984-present
Member, Pediatric Oncology Group (now Children’s Oncology Group), 1987
Board Certified, Clinical Cytogenetics, American Board of Medical Genetics, 1987
Election to Sigma Xi Scientific Research Society, 1987
Associate Professor, Department of Pediatrics, Division of Medical Genetics, 1991-2001
Founding Fellow, American College of Medical Genetics, 1991
Secretary-Treasurer, North Carolina Medical Genetics Association, 2000-2001
Professor, Department of Pediatrics, Division of Medical Genetics, 2001-present
Vice-Chair, North Carolina Medical Genetics Association, 2001-2002
Chair, North Carolina Medical Genetics Association, 2002-2003
Interim Division Head, Division of Medical Genetics, Department of Pediatrics, 2006-Present
B. Selected peer-reviewed publications (in chronological order). Do not include publications submitted or in
preparation.
1. Wiley, J.E. 1982. Chromosome banding patterns of treefrogs (Hylidae) of the eastern United States. Herpetologica. 38(4):507-520.
2. Wiley, J.E., L.M. Sargent, S. Inhorn, and L.F. Meisner. 1984. Comparison of prometaphase chromosome techniques with special emphasis
on the role of colcemid. In Vitro 20(12):937-941.
3. Wiley, J.E. and L.F Meisner. 1984. Synergistic effect of TPA and T-cell mitogens in non-mammalian vertebrates. In Vitro 20(12):932-936.
4. Kushnick, T., Irons, T.G, Wiley, J.E., Gettig, E.A., Rao, K.W., and S. Bowyer. 1987. 45X/46,r(X) with syndactyly and severe mental
Retardation. Amer. J. Med. Genet. 28:567-574.
5. Zhang, R., Wiley, J.E., Howard, S.P., Meisner, L.F., and M.N. Gould. 1989. Rare clonal karyotypic variants in primary cultures
of human breast carcinoma cells. Cancer Res. 49:444-449.
6. Wiley, J.E., Little, M.L., Romano, M.A., Blount, D.A., and G.R. Cline. 1989. Polymorphism in the location of the 18S and 28S rRNA genes on
the chromosomes of the diploid-tetraploid treefrogs Hyla chrysoscelis and H. versicolor. Chromosoma 97:481-487.
7. Meyne, J., Baker, R.J., Hobart, H.H., Hsu, T.C., Ryder, O.A., Ward, O.G., Wiley, J.E., Wurster-Hill, D.H., Yates, T.L., and R.K. Moyzis. 1990.
Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes. Chromosoma 99:3-10.
8. Goolsby, C.L., Wiley, J.E., Steiner, M., Bartholdi, M.F., Cram, L.S., and P.M. Kraemer. 1991. Karyotype evolution in a SV40
Transformed tumorigenic human cell line. Cancer Genet. and Cytogenet. 49:231-248.
8. Kushnick, T., Wiley, J.E. and S. Palmer. 1992. Agonadism in a 46,XY patient with CHARGE association. Amer. J. Med. Genet.
42:96-99.
PHS 398 (Rev. 05/01)
Page _______
Biographical Sketch Format Page
Use ½-inch MARGINS. Number pages consecutively at the bottom throughout the application. Do not use suffixes such as 3a, 3b.
9. Migeon, B.R., Luo, S., Stasiowski, B., Jani, M., Axelman, J., VanDuyke, D., Weiss, L., Jacobs, P., Yang-Feng, T and J. Wiley. 1993. Deficient
transcription of XIST in individuals with tiny ring X chromosomes and severe phenotypes: implications for the role of XIST in X inactivation.
Proc. Nat. Acad. Sci. 90:12025-12029.
10. Sargent, L.M., Dragan, Y.P., Bahnub, N., Wiley, J.E., Sattler, C.A., Schroeder, P., Sattler, G.L., Jordan, V.C., and H.C. Pitot. 1994. Tamoxifen
induces hepatic aneuploidy and mitotic spindle disruption after a single in vivo administration to female Sprague-Dawley rats. Cancer Res.
54:3357-3360.
11. Wiley, J., Posekany, K., Riley, R., Holbrook, T., Silverman, J., Joshi, V, and S. Bowyer. 1995. Cytogenetic and flow cytometric analysis of a
pancreatoblastoma. Cancer Genet. and Cytogenet. 79:115-118.
12. Wiley, J.E., Stout, J.C., Palmer, S.M., and T. Kushnick. 1995. De novo nonreciprocal translocation 1;8 confirmed by fluorescent in situ
hybridization. Amer. J. Med. Genet. 57:579.
13. Sasser, E.W., Wiley, J.E., and C. Daeschner. 1995. Acute lymphoblastic leukemia in a patient with familial derivative chromosome 4. Cancer
Genet. and Cytogenet. 82:90-91.
14. Burke, L.W., Wiley, J.E., Gleen, G.C., Driscoll, D.J., Loud, K.M., Smith, A.W., and T. Kushnick. 1996. Familial cryptic translocation resulting
in Angelman syndrome: implications for imprinting or location of the Angelman gene? Am. J. Hum. Genet. 58:777-784.
15. Bonner, D.S., Wiley, J.E. and M.A. Farwell. 1998. Assignment of the mitochondrial translation initiation factor 2 gene (MTF2) to human
chromosome 2 bands p14->p16 by in situ hybridization and with somatic cell hybrids. Cytogenet. Cell Genet. 83:80-81.
16. Wiley, J.E. and M.L. Little. 2000. Replication banding patterns of the diploid-tetraploid treefrogs Hyla chrysoscelis and H. versicolor.
Cytogenet. Cell Genet. 88:11-14.
17. Vernon, J.L., Burr, P.C., Wiley, J.E., and M.A. Farwell. 2000. Assignment of the mitochondrial translation elongation factor Ts gene (TSFM)
to human chromosome 12 bands q13->q14 by in situ hybridization and with somatic cell hybrids. Cytogenet. Cell Genet. 89:145-146.
18. Wiley, J.E., Madigan, M., Christie, J.D. and A.W. Smith. 2002. Dispermic chimerism with two abnormal cell lines, 47,XY,+21 and 47,XX,+12.
Amer. J. Med. Genet. 107:64-66.
19. Overman, G.R., Enderle, P.J., Farrow, J.M., Wiley, J.E., and M.A. Farwell. 2003. The human mitochondrial translation initiation factor 2 gene
(MTIF2): transcriptional analysis and identification of a pseudogene. Biochim. Biophys. Acta 1628:195-205.
20. Wiley, J.E. 2003. Replication banding and FISH analysis reveal the origin of the Hyla femoralis karyotype and XY/XX sex chromosomes.
Cytogenet. Genome Res. 101:80-83
21. Keelean, D., Pierpont, J., Wiley, J., and K. McGinty. 2004. Birth Defects. In L. A. Rapp-Paglicci, C. N. Dulmus, J. S. Wodarski (Eds.)
Handbook of Preventive Interventions for Children and Adolescents, pp.133-148. Hoboken, New Jersey: John Wiley and Sons.
22. Ojeda, J. F., Xie, C., Li, Y-Q., Bertrand, F. E., Wiley, J., and McConnell, T. J. 2006. Chromosomal analysis and identification based on
optical tweezers and Raman spectroscopy. Opt. Exp. 14:5385-5393.
Bonde, P., Sui, G., Shara, S., Wang, J., Broor, A., Kim, I., Wiley, J.E., Marti, G., Duncan, M., Jaffee, E.,
Montgomery,E.,Maitra, A., and J.W. Harmon. 2007. Cytogenetic characterization and gene expression profiling in the rat
reflux induced esophageal tumor model. J. of Thorac.and Cardiovas. Surg. 133:763-769.
23.
24. Dudek, R.W. and J.E. Wiley. 2008. Hi-Yield Genetics. Lippincott, Williams & Willins.
C. Research Support. List selected ongoing or completed (during the last three years) research projects (federal and
non-federal support). Begin with the projects that are most relevant to the research proposed in this application. Briefly
indicate the overall goals of the projects and responsibilities of principal investigator identified above.
. Ongoing Research Support
Research Development Award, East Carolina University
7/01/08-6/30/09
John E. Wiley (PI) and Gregory Gagnon (Co-PI), $29,058.00
Optimization and Elucidation of Immune Responses Against Leukemo/Lymphogenic Fusion Proteins Derived
from Chromosomal Rearrangements via Labeling with the Gal Tumor Rejection Antigen.
North Carolina Department of Health and Human Services
John E. Wiley (PI), $254,615
Genetic Services
7/01/06-6/30/09
Completed Research Support
Study 5-63851 Wiley (PI)
5/01/01-5/01/03
Epigenesis Corporation, $10,000
In vitro cytogenetic study: effects of EPI=12322 and EPI-12324 alone and in combination on abnormalities caused by
known clastogenic agents-dose response and effect.
The goal of the study was to evaluate the anti-clastogenic effects of the two drugs alone and in combination in cultures of
human bronchial epithelial cells.
Role: PI
2000 line item Wiley (PI)
7/01/05-6/30/06
Department of Pediatrics Graduate student research support
Role: Dissertation research director
Research Development Award, East Carolina University
7/01/06-6/30/07
Yong-qing Li, PI, John E. Wiley, Fred Bertrand, Tom McConnell, Co-PI’s
$24000, “New Approaches to Cancer Detection and Diagnosis Using Tweezers Spectroscopy”.