Table 1. Overview of K. pneumoniae proteins identified after in-gel digestion with trypsin and MALDI-TOF MS and/or ESI-QqTOF
MS/MS analysis
Function
Category
Spot
noa
Carbohydrate
metabolism
5
dha regulon related
39
Annotation and/or
Homologue Proteins
Glycerol dehydrogenase (EC 1.1.1.6)
(GLDH)sp|P45511|GLDA_CITFR
97
Dihydroxyacetone kinase I (EC
2.7.1.29)sp|P45510|DAK_CITFR
42 Dihydroxyacetone kinase II, subunit 1 (EC
114 2.7.1.121)sp|P76015|YCGT_ECOLI
18 Dihydroxyacetone kinase II, subunit 2 (EC
153 2.7.1.121)sp|P76014|YCGS_ECOLI
33 Glycerol dehydratase beta subunit (EC 4.2.1.30)
tr|O08505[Klebsiella pneumoniae]
62 glycerol dehydratase small subunit (EC 4.2.1.30)
tr|Q59475
12 1,3-propanediol dehydrogenase (EC 1.1.1.202)
sp|Q59477|DHAT_KLEPN
35
36
103
107
6 Hypothetical oxidoreductase yqhD (EC 1.1.-.)sp|Q46856|YQHD_ECOLI
pI
KPNb
862
Pm/Psd
SCe
(%)
121
17/28
39
219 LAAQAGVVTEALER 232
11 YLQGPDAAVLFGQYAK 26
164 LLVSGF(M)GDALSTWFEAK 180
41.2
136
11/15
34
146 NPDF(M)VVMDTAIIAK 159
219 LAAQAGVVTEALER 232
11 YLQGPDAAVLFGQMC(YA)K 26
5.23
332
5.03
5.16
52.0 50.2
131
15/31
31
863
4.86
855
4.91
4.85
5.70
5.47
6.72
6.53
39.1 38.3
39.0
24.8 22.7
25.8
24.3 21.3
102
89
152
103
71
13/37
11/31
12/17
12/30
11/35
42
29
77
60
46
854
6.66
8.12
16.8 16.3
96
7/15
52
858
5.91
5.94
41.8 41.5
105
15/37
36
3405
5.81
5.84
5.53
5.70
5.32
5.28
42.0
41.8
43.4
42.5
40.2 42.3
175
63
114
184
199
23/58
13/64
17/60
20/43
17/24
48
26
38
41
40
40.1
130
13/25
29
40.1
39.8 39.0
19.8 15.4
119
74
81
15/41
12/50
6/25
37
42
48
864
40
117
129 Putative glycerol dehydrogenasetr|Q8ZR27
151 orfY, unknown function
gi|940439|(U30903)[Klebsiella pneumoniae]
Mr (kDa) Scorec
2- pred 2- pred
DE
DE
5.31 5.25 41.4 39.0
5.67
5.25
2195
857
5.18
5.40
4.90
5.41
4.94
Partial amino acid sequencef
and/or comments
285 LGATPLSELYGVYNR 299
3 QTTQIQ 8
233 QAVALGSNLQAR 244
94 DIIVTVGGGSPT(H)D 106
343 LSDYGIDGSSIPALLAK 359
311 VWNITEGSDDQR 322
172 FAILDPVYTYTL 183
231 ALQEPENYNVR 241
343 VT(LS)DYGLDGSSIPALLAK 359
312 WNITEGSDD 320
Identified by NCBInr search and
confirmed by Knexus search (Z% is 84)
1
Glycolysis/
gluconeogenesis
87
Phosphoglucomutase (EC 5.4.2.2) (Glucose
phosphomutase) (PGM)sp|P36938|PGMU_ECOLI
15
Fructose-bisphosphate aldolase class II (EC 4.1.2.13)
sp|P11604|ALF_ECOLI
17 Triosephosphate isomerase (EC 5.3.1.1) (TIM)
140 sp|Q9Z6B9|TPIS_ENTCL
19 Glyceraldehyde 3-phosphate dehydrogenase (EC
1.2.1.12)P24164_G3P_KLEPN
30
31
135
4 Phosphoglycerate kinase (EC 2.7.2.3)
sp|Q8XG18|PGK_SALTY
41
112
29 Phosphoglycerate mutase 1 (EC 5.4.2.1)
sp|P31217|PMG1_ECOLI
91 Phosphoglyceromutase (BPG-independent PGAM)
(EC 5.4.2.1) sp|P37689|PMGI_ECOLI
2 Enolase (EC 4.2.1.11) sp|P08324|ENO-ECOLI
3
51
98
85
Pyruvate kinase I (EC 2.7.1.40)
sp|P77983|KPY1_SALTY
818
5.30
5.37
57.2 58.6
72
12/39
22
482 LTAAPGD(N)GAAIGGLK 496
463 LSPEF(M)VSADTLAGDPITAR 481
253 LNLTIVNDQVDQTFR 267
2258
5.69
5.67
39.0 39.1
82
12/34
49
309 AYLQGQLGNPK 319
58 IVFSD(N)GGAAFIAGK 72
5084
5.78
1223
5.70
5.54
6.57
6.67
31.5 26.9
32.1
35.5 35.5
114
55
125
20/66
8/42
16/31
59
23
44
2259
6.25
6.07
5.91
5.14
5.02
35.6
35.9
36.2
41.6 40.5
55
73
77
216
14/80
13/38
15/64
21/29
45
36
46
66
5.07
41.6
159
23/43
66
124 YAALCDV(EMN)FVMDAFGTAHR 141
g75
4.99
5.62
5.86
41.6
32.3 28.4
112
262
13/26
25/62
32
82
161 VVPYWNETILPR 172
5033
5.33
5.66
54.0 51.7
102
15/44
37
g1949
5.28
5.33
46.5 45.5
115
13/33
56
g4554
5.22
5.17
5.09
5.61
5.66
46.1
45.9
46.0
58.4 50.7
65
58
56
53
13/32
12/46
7/22
7/32
48
32
26
24
58.7
282
4/24
19
64.9 72.7
65.4
36.0 35.4
36.4
36.1
57
178
107
86
69
9/23
6/38
14/49
12/48
10/44
15
10
48
38
29
84
5.52
308 LVSWYDNETGYSNK 321
297 AGIALNDNFVK 307
201 AQNIIPSSTGAAK 213
229 YEADLVDEAK 238
254 VATEFSETATATLK 267
204 DQLIVGGGIANTFVA 218
283 EDATIADLAVGTAAGQIK 300
319 IQ(E)EALGEQAPFD(N)GR 332
248 GVEIPVEEV(G)I 257
303 LDGTDAVF(M)LSGESAK 317
304 VANAL(V)LDGTDAVF(M)
LSGESAK(AETA) 322
248 GVEIPVEEV(G)I 257
390 YFPDATILALTT 401
Pentose pathway
81
82
123
121
122
Transketolase 1 (EC 2.2.1.1) (TK 1)
sp|P27302|TKT1_ECOLI
2261
Transaldolase B (EC 2.2.1.2)
sp|P30148|TALB_ECOLI
760
5.48
5.52
4.95
5.07
5.04
5.67
4.99
533 TAEQLANVAR 542
321 AFPQEAAEFTR 331
2
EntnerDoudoroff
pathway
149 KHG/KDPG aldolase sp|P10177|ALKH_ECOLI
421
5.41
5.50
28.5 22.4
87
11/39
58
TCA cycle
80
4087
5.66
5.83
65.6 66.0
114
17/43
30
2729
5.49
5.54
36.7 32.6
121
19/74
53
4159
5.37
5.02
70.2 70.2
67
13/46
19
2114
5.09
5.15
1282
4.87
5.13
5.06
4.77
4.70
17.9 14.9
44.9 42.0
44.1
17.0 12.0
63
78
86
58
5/33
12/40
13/35
5/21
69
28
26
52
4317
5.25
5.24
31.4 27.2
104
12/27
45
h2981
5.25
+2978
+2979
+2980
653 5.72
5.29
42.6 42.8
69
8/38
31
5.73
52.5 55.2
132
26
55
4.89
54.5
54.0
48.0 50.2
208
150
208
20/25
18/34
24/29
49
31
48
Acetate
formation
One-Carbon
metabolism
Hydrogen
transport system
Others
Energy Metabolism
 ATP synthesis
Fumarate reductase flavoprotein subunit (EC
1.3.99.1) sp|P00363|FRDA_ECOLI
133 Malate dehydrogenase (EC 1.1.1.37)
sp|Q60150|MDH_ECOLI
79 Phosphate acetyltransferase (EC 2.3.1.8)
sp|P39184|PTA_ECOLI
168 Protein yfiD sp|P33633|YFID_ECOLI
72 S-adenosylmethionine synthetase (EC 2.5.1.6)
99 sp|P04384|METK_ECOLI
163 Thioredoxin 1 sp|P00274|THIO_ECOLI
146 2-deoxy-D-gluconate 3-dehydrogenase (EC
1.1.1.125) sp|P37769|KDUD_ECOLI
113 Mannose-6-phosphate isomerase (EC 5.3.1.8)
sp|P00946|MANA_ECOLI
10
48
93
43
ATP synthase alpha chain (EC 3.6.3.14)
sp|P00822|ATPA_ECOLI
ATP synthase beta chain (EC 3.6.3.14)
sp|P00824|ATPB_ECOLI
g3124
655
5.58
5.48
4.92
16 IAQFNVVSEAHNEGTIVSVS 35
402 ELAAFSQFASDLD 414
1 MQLNSTEQ(I)SN(E)LIK 13
346 DPLVVGQEHYDTAR 359
248 YTLAGTEVSALLGR 261
115 PSYEELSSSQELLETGIK 132
346 DPLVVGQEHYD 356
168 AIEHGVSYS(SGYSV)FAGVGER 183
248 YTLAGTEVSALL 259
69
Phosphate
metabolism
Sulfur
metabolism
Amino Acids
Metabolism
Aspartate family
Lysine,
ATP synthase gamma chain (EC 3.6.3.14)
sp|P00837|ATPG_ECOLI
58 Inorganic pyrophosphatase (EC 3.6.1.1)
sp|Q8XGI0|IPYR_SALTY
144 3-mercaptopyruvate sulfurtransferase (EC 2.8.1.2)
sp|P31142|THTM_ECOLI
105 Aspartate-semialdehyde dehydrogenase (EC
1.2.1.11) sp|P00353|DHAS_ECOLI
37
2,3,4,5-trtrahydropyridine-2-carboxylate N-
654
8.55
9.11
33.1 31.6
99
12/32
38
2803
4.86
4.90
24.3 19.8
50
5/18
40
4761
4.57
4.63
34.2 31.3
61
11/56
38
3774
5.52
5.64
41.2 40.1
72
14/38
34
919
5.40
5.41
36.1 30.0
231
23/43
62
3
diaminopimelate
Threonine,
Glycine, Serine
Cysteine
125 succinyltransferase sp|P03948|DAPD_ECOLI
9 Threonine synthase (EC 4.2.3.1)
sp|P00934|THRC_ECOLI
109 2-amino-3-ketobutyrate coenzyme A ligase (EC
2.3.1.29) sp|P07912|KBL_ECOLI
131 Phosphoserine aminotransferase (EC
2.6.1.52)(PSAT) sp|P55900|SERC_SALTY
55 Serine hydroxymethyltransferase (EC 2.1.2.1)
sp|P06192|GLYA_SALTY
142 Phosphoadenosine phosphosulfate reductase (EC
1.8.4.8) sp|P17854|CYSH_ECOLI
16 Cysteine synthase A (EC 4.2.99.8)
sp|P11096|CYSK_ECOLI
14
166
Valine, isoleucine
Leucine
Arginine
Histidine
83
Dihydroxy-acid dehydratase (EC 4.2.1.9) (DAD)
sp|P40810|ILVD_SALTY
86 Acetolactate synthase isozyme I large subunit (EC
4.1.3.18) sp|P08142|ILVB_ECOLI
95 3-isopropylmalate dehydratase large subunit (EC
4.2.1.33) sp|P30127|LEU2_ECOLI
106 Carbamoyl-phosphate synthase small chain (EC
6.3.5.5) sp|P14845|CARA_SALTY
8 Argininosuccinate synthase (EC 6.3.4.5)
sp|P22767|ASSY_ECOLI
101
134 ATP phosphoribosyltransferase (EC 2.4.2.17)
sp|P00499|HIS1_SALTY
549
5.28
5.48
5.47
36.1
43.8 46.7
203
61
19/31
11/39
49
33
5039
5.74
5.65
40.8 43.5
144
19/48
38
3552
5.46
5.44
38.9 39.9
92
17/63
50
4788
5.99
6.47
43.3 45.5
107
10/16
40
1956
5.76
5.81
31.6 27.8
188
18/36
47
h3706 5.79
+3707
+3708
+3709
5.84
37.1 34.4
56
32
6/29
3/29
47
57
32
3/29
65
173
19/29
55
63
20
36
6/21
2/21
3/21
51
25
65
147
15/21
47
56
28
5/28
3/28
51
28
h3706 5.60
+3707
+3709
37.8
h3706 5.46
+3707
38.1
483
5.49
5.53
61.9 66.3
132
142
13/28
17/31
41
23
2156
5.24
5.18
56.5 59.6
89
14/35
24
4862
6.16
6.18
47.9 50.2
132
14/27
34
4818
5.63
5.08
41.9 21.3
93
8/19
35
2886
5.44
5.43
46.0 49.9
78
9/15
27
4013
5.39
5.68
5.76
46.0
36.4 33.5
78
110
17/68
14/41
40
46
271 AATNANDTVPR 281
15 ALGANLVLTEGAK 27
18 IQGIGAGFIP 27
1 MPETMSIER 9
Confirmed by NCBInr search and
by Knexus search (Z% is 99)
18 IQGIGAGFIPFH(GNL)DLK 33
15 ALGANLVLTE 24
Confirmed by NCBInr search and
by Knexus search (Z% is 99)
Confirmed by NCBInr search and
by Knexus search (Z% is 99)
246 YYEQDDASALPR 257
454 VYESQDDAVEAILGGK 469
337 FDSQALLMR 349
44 H(N)LGQPDEDDYDAIPR 58
429 SASSATGLPQVENLENK 445
4
Phenylalamine,
Tyrosine and
Tryptophan
Tryptophan
Nucleotide and
Deoxyribo-nucleotide
Metabolism
Coenzyme and Vitamin
NAD
biosynthesis
Vitamin C
Lipid Metabolism
Fatty acid
biosynthesis
Liposaccharide
biosynthesis
Genetic Information
Processing
DNA
RNA
transcription
Transcription
regulation
Posttranscriptional
processing
Protein
Translation
167 3-deoxy-D-arabino-heptulosonate 7-phosphate
synthase (EC 4.1.2.15) sp|p00888|AROF_ECOLI
h876
+875
5.74
5.42
39.9 38.8
145 Tryptophan synthase alpha chain
sp|P00930|TRPA_KLEAE
111 Tryptophan synthase beta chain (EC 4.2.1.20)
sp|P00932|TRPB_ECOLI
100 Phosphopentomutase (EC
5.4.2.7)(Phosphodeoxyribomutase)
sp|P07651|DEOB_ECOLI
127 Ribose-phosphate pyrophosphokinase (EC 2.7.6.1)
(RPPK) sp|P15849|KPRS_SALTY
143 Uridine phosphorylase (EC 2.4.2.3) (UDRPase)
sp|O33808|UDP_SALTY
128 NH(3)-dependent NAD(+) synthetase (EC 6.3.5.1)
(Nitrogen-regulatory protein)
sp|P18843|NADE_ECOLI
21 2,5-diketo-D-gluconic acid reductase A (EC 1.1.1.-)
sp|Q46857|DKGA_ECOLI
96 Biotin carboxylase (EC 6.3.4.14) (A subunit of
acetyl-CoA) sp|P24182|ACCC_ECOLI
136 Enoyl-[acyl-carrier-protein] reductase [NADH](EC
1.3.1.9) sp|P29132|FABI_ECOLI
139 2-dehydro-3-deoxyphosphooctonate aldolase (EC
4.1.2.16) sp|P17579|KDSA_ECOLI
54 Single-strand binding protein (SSB)
sp|P02339|SSB_ECOLI
56 Chaperone protein DnaK (Heat shock protein)
sp|Q8Z9R1|DNAK_SALTI
116 DNA-directed RNA polymerase alpha chain (EC
2.7.7.6) sp|P00574|RPOA_ECOLI
159 Transcription elongation factor greA
sp|Q8XGQ9|GREA_ SALTY
154 Flavoprotein wrbA (Trp repressor binding protein)
sp|P30849|WRBA_ECOLI
76 Polynucleotide phosphorylase
(EC 2.7.7.8) (PNPase) sp|P05055|PNP_ECOLI
g, h70
5.00
5.04
+372
371
6.04
791
44
7
Trigger factor (TF) sp|Q8XFC4|TIG_SALTY
Elongation factor TU (EF-TU)
sp|P21694|EFTU_SALTY
Confirmed by NCBInr search and
confirmed by Knexus search (Z% is 82)
31.2 28.7
39
23
69
105
4/15
3/15
7/15
13/42
17
11
14
46
5.98
41.8 43.2
140
21/51
47
5.20
5.07
43.8 44.6
107
12/26
26
g4399
5.38
5.23
37.0 34.1
70
11/43
44
1574
5.88
5.89
31.4 27.2
62
12/61
50
1471
5.40
5.38
37.0 30.6
126
15/48
65
3406
5.92
5.98
31.9 30.8
176
21/59
76
125
6.48
6.10
45.5 46.3
242
23/35
51
1173
5.46
5.43
35.0 28.1
61
6/16
22
2003
5.90
5.89
33.7 31.0
128
11/20
30
5156
5.47
5.45
23.9 18.7
78
13/71
61
766
4.84
4.83
61.7 69.1
124
13/18
27
2345
5.02
4.99
39.7 32.4
68
12/62
43
2897
4.68
4.76
21.3 17.8
50
6/36
52
2234
5.87
5.84
21.2 20.9
79
9/27
41
2878
5.14
5.08
70.0 76.8
109
23/68
33
h439
4.87
4.83
51.8 48.2
241
31/70
69
115 NVALEEQAVEAVLAK 129
+438
f66
5.43
5.3
42.4 43.2
133
16/49
51
60 GITINTSHVEYDTW(P)T 74
Identified by NCBInr search and
confirmed by Knexus search (Z% is 99)
5
13
Elongation factor Ts (EF-Ts)
sp|Q8XGS0|EFTS_SALTY
120
121
122
77 Elongation factor G (EF-G) sp|P02996|EFG_ECOLI
78
64
61
63
50S ribosomal protein L6 sp|P02390|RL6_ECOLI
50S ribosomal protein L9 sp|Q8Z163|RL9_SALTI
50S ribosomal protein L10 (L8)
sp|P17352|RL10_SALTY
27 50S ribosomal protein L7/L12 (L8)
sp|P18081|RL7_SALTY
161 50S ribosomal protein L19 sp|P02420|RL19_ECOLI
24 30S ribosomal protein S6 sp|P02358|RS6_ECOLI
915
g48
+128
5.13
5.13
5.07
5.04
5.23
5.15
36.2 30.4
169
15/29
50
5.24
37.4
36.4
36.1
71.2 74.5
198
153
131
47
56
122
22/48
17/48
15/44
8/61
11/61
24/61
55
55
47
41
27
33
75
38
54
10/53
7/53
12/44
35
20
17
g48
+128
5.27
223
2827
1206
8.84
6.16
8.50
9.71
6.19
9.04
24.0 18.9
17.9 15.8
18.5 17.8
102
193
167
7/11
16/34
20/67
39
85
82
1207
4.53
4.60
17.2 12.3
-
-
-
882
4.63 10.62 18.6 13.1
5.31 4.93 18.7 15.7
57
90
5/22
7/26
48
57
1372
5.25
5.49
5.50
18.7
37.3 36.8
65
171
6/36
19/47
34
57
3521
5.51
5.52
51.7 48.8
75
6/8
13
2353
4.95
5.03
21.2 18.9
53
6/31
24
2767
5.69
5.70
20.0 18.2
107
10/31
50
1503
5.30
5.59
50.3 53.2
129
18/41
45
2718
5.53
5.57
42.3 42.9
70
10/34
24
2636
5.32
5.25
5.53
5.23
53.6 52.6
53.4
45.3 46.5
74
12/38
32
h2830
71.5
26 ALTEAD(N)GDIELAIENMR 42
227 VSLTGQPFVMEPSK 240
323 IATDPFVGNLTFFR 335
Confirmed by NCBInr search and by
Knexus search (Z% is 96)
Confirmed by NCBInr search and by
Knexus search (Z% is 87)
110 TV(SL)EEAGAEL(V)EVK 12
+2829
Posttranslational
processing
Protein
degradation
162
132 Phenylalanyl-tRNA synthetase alpha chain (EC
6.1.1.20) sp|P08312|SYFA_ECOLI
94 Seryl-tRNA synthetase (EC 6.1.1.11)
sp|P09156|SYS_ECOLI
157 Peptide deformylase (EC 3.5.1.88)
sp|P27251|DEF_ECOLI
156 Peptidyl-prolyl cis-trans isomerase B (EC 5.2.1.8)
sp|P23869|PPIB_ECOLI
46 Outer membrane protein tolC precursor
sp|P02930|TOLC_ECOLI
104 Protease degQ precursor (EC 3.4.21.-)
sp|P39099|DEGQ_ECOLI
90 Aminoacyl-histidine dipeptidase (EC 3.4.13.3)
89 sp|P15288|PEPD_ECOLI
102 Peptidase B (EC 3.4.11.-) (Aminopeptidase B)
sp|Q9RF52|PEPB_SALTY
4765
5.57
263 YATVAVAADQ(K)ADALK 277
167
20/40
45
6
Environmental
Information Processing
Membrane
transport
53
Periplasmic oligopeptide-binding protein precursor
sp|P23843|OPPA_ECOLI
1395
5.85
6.14
55.8 61.4
195
31/65
57
71
Periplasmic dipeptide transport protein precursor
sp|P23847|DPPA_ECOLI
Putative biding protein yLiB precursor
sp|P75797|YLIB-ECOLI
Glycine betaine-binding periplasmic protein
precursor sp|P14177|PROX_ECOLI
Leu/Ile/Val-binding protein precursor(LIV-BP)
sp|P02917|LIVJ_ECOLI
ABC transporter periplasmic binding protein ytfQ
precursor sp|P39325|YTFQ_ECOLI
D-methionine-binding lipoprotein metQ precursor
sp|P28635|METQ_ECOLI
Histidine-binding periplasmic protein precursor
(HBP) sp|P02910|HISJ_SALTY
PTS system, glucose-specific IIA component (EIIAGLC) sp|P02908|PTGA_SALTY
Autoinducer-2 production protein luxS (EC3.13.1.-)
sp|Q9L4T0|LUXS_SALTY
Betaine aldehyde dehydrogenase (EC 1.2.1.8)
(BADH) sp|P17445|DHAB_ECOLI
Stringent starvation protein A
sp|P05838|SSPA_ECOLI
N-ethylmaleimide reductase
(EC 1.-.-.-) sp|P77258|NEMA_ECOLI
834
5.97
6.01
50.7 59.2
109
22/58
37
4661
8.20
8.74
48.5 56.4
119
15/24
24
1055
5.56
5.44
38.0 35.5
39
8/29
27
3798
5.68
5.69
41.1 37.4
66
6/11
17
3991
5.44
5.42
39.5 34.4
105
15/57
45
3459
5.60
5.97
33.7 28.8
122
12/28
36
1828
5.60
6.03
35.4 28.3
127
15/45
53
3713
4.71
4.73
21.1 18.2
106
9/22
43
1047
5.16
5.18
22.2 19.6
111
11/30
55
2152
5.23
5.09
54.6 52.7
98
15/40
26
1147
5.29
5.22
29.9 24.2
121
13/29
56
h733
6.06
5.81
39.9 39.5
71
10/38
56
44
5/38
47
70
73
108
130
137
115
158
quorum sensing
152
Stress response
88
148
34
1
28
11
47
92
59
22
32
Alkyl hydroperoxide reductase C22 protein (EC
1.6.4.-) sp|P19479|AHPC_SALTY
Alkyl hydroperoxide reductase subunit F (EC 1.6.4.-)
sp|P19480|AHPF_SALTY
Putative thiol-alkyl hydroperoxide reductase (EC
1.6.4.-) tr|Q8XGE8
Superoxide dismutase [Fe] (EC 1.15.1.1)
sp|P40726|SODF_SALTY
Superoxide dismutase [Mn] (EC 1.15.1.1)
sp|P00448|SODM_ECOLI
+732
+731
3981
5.05
3980
4.92
5.44
5.03
23.4 20.7
145
16/36
78
5.4
23.4
55.5 56.0
109
139
12/22
20/40
49
41
2517
5.39
5.33
5.11
5.15
55.3
56.0
25.8 22.4
97
84
112
19/50
14/38
15/54
37
29
55
724
5.70
5.76
23.4 21.2
67
6/17
33
3668
6.15
6.23
27.6 23.0
141
13/32
43
241 LAD(N)GANYGFPVNTMHIVANK
260
4 ALETAEIPGIVNDFR 18
172 EGEATLAPSLDLVG 185
94 YAMIGDPTGALTR 106
507 ASLSAFDYLIR 517
293 LTPAATEGGLHQIETAS 309
7
10 kDa chaperonin (Protein Cpn10) (groES protein)
sp|P05380|CH10_ECOLI
60 kDa chaperonin (Protein Cpn60) (groEL protein)
(HSP60KP) sp|O66026|CH60_KLEPN
4105
5.38
5.39
18.6 10.4
140
11/27
73
48 ILENGTVQPLDVK 60
21 SAGGIVLTGSAAAK 34
4104
4.87
4.84
55.9 57.1
209
33/58
53
426 LAGLTGQNEDQN 437
145 AQVGTISANSDEEA(TV)GK 160
83 DG(A)AAPTLSMG
(GDGTTTA)TVLAQAIVNEGLK 105
160 Putative molecular chaperone (Small heat shock
protein)tr|Q8ZPY6
150 GrpE protein (HSP-70 cofactor) (Heat shock protein
B25.3) sp|P09372|GRPE_ECOLI
26 16 kDa heat shock protein A
164 sp|P29209|IBPA_ECOLI
147 Phage shock protein A sp|P23853|PSPA_ECOLI
138 Hypothetical oxidoreductase yajO (EC 1.-.-.-)
sp|P77735|YAJO_ECOLI
141 Probable oxidoreductase ydfG (EC 1.-.-.-)
sp|P39831|YDFG_ECOLI
38 outer membrane protein A precursor
sp|P2401|MPA_KLEPN
49 CLPB protein (heat shock protein F84.1)
50 sp|P03815|CLPB_ECOLI
60 Protein yifE sp|P27827|YIFE_ECOLI
67 Protein yajQ sp|P77482|YAJQ_ECOLI
68 Protein ydgH Precursor sp|P76177|YDGH_ECOLI
65 Histone-like protein HLP-1 precursor
sp|P11457|HLPA_ECOLI
75 Putative outer membrane protein Vprtr|Q8KR94
118 Protein StbA (ParA locus 36 kDa protein)
sp|P11904|STBA_ECOLI
119 Outer membrane protein A precursor
124 sp|P24017|OMPA_KLEPN
126 Hypothetical protein yfeX sp|P76536|YFEX_ECOLI
155 Hypothetical protein yqjDsp|P42617|YQJD_ECOLI
3068
5.40
5.56
19.3 17.6
110
9/23
38
183
4.69
4.69
28.4 21.7
112
11/27
45
4204
5.40
4350
3045
5.33
5.05
5.38
5.76
5.37
5.78
17.4 15.7
17.1
30.8 25.4
34.3 34.2
112
112
142
154
10/26
6/33
14/38
17/37
45
40
51
47
3073
5.73
5.71
32.3 27.2
125
18/46
65
2588
5.43
5.42
38.6 31.0
99
16/79
57
866
5.48
339
946
2993
706
5.43
5.41
5.93
5.49
8.59
8.82
5.95
5.60
9.19
9.43
72.4
72.4
17.9
21.1
35.5
20.8
13.2
18.3
34.0
16.5
78
96
145
212
116
107
12/26
19/61
10/21
20/52
11/21
8/17
24
30
77
86
37
52
704
5214
5.09
5.03
5.14
5.02
74.8 79.5
38.2 35.3
47
89
8/23
10/28
11
32
2588
5.14
5.30
5.31
5.41
5.42
38.4 31.0
38.6
36.7 33.2
20.5 11.0
97
119
115
91
10/25
12/28
13/34
6/13
36
47
65
41
20
45
Un-categorized
3723
1639
5.27
5.56
75.1
75 GYPITDDLDIYTR 87
65 ALFATGNFEDVR 76
a Refers
to the proteins labelled in Figure 1
Protein access numbers in the ProtKpn database
c Score is -10*Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 50 are significant (p<0.05) for searching in ProtKpn database, and scores
greater than 64 (italic marked) for searching in NCBInr database. If there is also a superscript number beside the score, it represents the position of this protein in the protein candidate list.
Otherwise, it is the top one.
d Pm and Ps: number of peptide mass values matched and searched, respectively
b
8
e SC=
Sequence coverage
Bold marked amino acids are from sequencing results whereas amino acids in parentheses are predicted in the ProtKpn database, italic marked are sequences originated from the homologue
protein given by searching in the database SWALL of the European Bioinformatics Institute.
g Refers to partial ORFs at the beginning or the end of contigs of Klebsiella pneumoniae. pI and Mr for such partial proteins are predicted by using the homologous protein from public database.
h Refers to the presence of gaps or sequence errors in DNA sequences leading to incorrect prediction of ORFs. pI and Mr for such partial proteins are predicted by using the homologous protein
from public database.
f
9