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Supplementary materials legends
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Supplementary table 1: List of differentially expressed genes in male and metestrous female
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GnRH neurons. In total, 543 genes showed differential expression at the following cut-off
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criteria: fold change >1.6 for 482 genes and <0.63 for 61 genes; adjusted p-value < 0.01;
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normalized expression value > 7 in the female.
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Supplementary table 2: Gene list of the top functional annotation clusters. List of
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differentially expressed genes were analyzed using a web-based public functional annotation
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clustering tool in DAVID Bioinformatics Resources and presented in fig. 2.
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Supplementary table 3. Validation of the microarray data by real-time PCR and
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comparison of microarray and qPCR results. For sampling of GnRH neurons, laser capture
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microdissection (LCM-qPCR; average RQ ± SEM) and patch-pipette cytoplasm harvesting
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(PCH-qPCR; average RQ ± SEM) approaches were followed by qPCR investigations that
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confirmed the differential expression of 46 genes in both samples (gene descriptions in bold
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letters). Abbreviations: FC, fold change; RQ: relative quantity; SEM: standard error of the mean.
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Supplementary fig. 1. Quantitative analysis and correlation of microarray raw data with
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real-time PCR results. The coefficient of determination value (R2 = 0.64) indicates a strong
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quantitative correlation between the normalized expression data of microarray hybridization
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studies and the cycle threshold (Ct) value of the qPCR investigations. X-axis: cycle threshold
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value, Y-axis: normalized microarray expression values (data are log2 transformed).
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