Curriculum Vitae Wael A. Sabra Personal data : Name: Date of Birth: Place of birth: Family status: Address: Tel No.: Mobile: E mail: Fax: Wael Ahmed Sabra 13.04.1967 Alexandria, Egypt married with three lovely children 21 El Amir Abd El Halim str, Viktoria, Alexandria 002 03 5756134 0127431385 waelsabra@web.de 002 03 - 3911794 Current position: Lecturer in the Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt. Postgraduate studies : 1989-1990 1991-1993 10.1993-3.1994 10.1995-10.1998 Postgraduate study at the Faculty of Science, Alexandria University, Alexandria, Egypt. M.Sc. Study in Microbiology in the Faculty of Science, Alexandria University, Egypt. M.Sc.: August, 1993 Training scholarship from DAAD in the Biochemical engineering division, GBF-National Research Institute for Biotechnology, Braunschweig, Germany. Ph.D. study in Biochemical engineering division, at the national research centre for biotechnology (GBF) in Braunschweig, Germany. Ph.D. Title ‘Microaerophilic Alginate production by Azotobacter vinelandii‘. Ph.D. December, 1998 (Grade very good) Employment : 1988-1993 08.1993-09.1995 10.1995-10.1998 1.1999- 3.1999 4.1999-5.1999 6.1999-12-2000 Demonstrator in Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt. Assistant lecturer in Botany and Microbiology Department, Faculty of Science, Alexandria University, Egypt Ph.D. student in the chemical engineering department under the supervision of Prof. Dr. Walt-Dieter Deckwer, GBF, Braunschweig, Germany Post-doctor Scholarship in GBF, Braunschweig, Germany: Title ‚Hydrodynamic stress in multiphase reactors using Alginate capsule as a model system’ Guest-scientist in GBF, Braunschweig, Germany Post-doctor position in GBF, Braunschweig, Germany: Title ‘Poly-ß- hydroxybuytrate production from Molasses‘ – Work in Pilot plant and no publication of results was permitted 1.2001-15.1.2003 8.2005 - 10.2005 Post-doctor position in GBF, Braunschweig, Germany: Title ‘Studying the virulence in Pseudomonas aeruginosa‘ Post doctoral; study in GBF, Braunschweig Germany: Title ‘small colony variant and wild type in Pseudomonas aeruginosa Teaching experience: - Teaching assistant, Biotechnology practical course in the International Training program. Institute for Biotechnology (GBF), Braunschweig, Germany. Teaching post-graduate students in the field of Biotechnology and Soil Bacteriology in Microbiology Department, Faculty of Science, Alexandria University. Teaching under-graduate students in Microbiology Department, Faculty of Science, Alexandria University in the following courses: General Microbiology Microbial Ecology Microbial Metabolism, Applied Mycology Fermentation equipment Molecular biology and genetic engineering. Plant anatomy Plant physiology Systematic botany - - Practical Qualification - Good experience with different fermentation techniques in laboratory scales. Praxis in the application of various methods of analysis including, 2D electrophoresis; proteomics, HPLC, GPC, GC and enzymatic assays Management capability and supervision of students, technical assistants as well as guest-scientists. Safety courses in handling gene modified or pathological micro-organisms. Other Qualifications - Very good English and German knowledge, both written and spoken Excellent knowledge of the usual computer programs (Ms-Office, Graphicprogramme, Internet applications) Other activities - Establishment of a fermentation biotechnology lab with donated equipments from Germany Funded Projects Principal investigator of project: Metabolic flux distribution and process optimisation of the pharmaceutically important polymannuronic polymer Funded by Program of the National Strategy for Biotechnology and Genetic Engineering, Egypt. 1998-2003 Member of the implementation team in the project named "Enhancement of Practical Skills of Botany, Microbiology and Biochemistry Students" which is funded by Higher Education Enhancement Project Fund (HEEPF). Scientific supervision of: 4 Master students and 1 Ph.D student Scientific consultant at the middle east technology company (Infection Control) Publications: - - - - - - - - - - - S. Sabry; K. Ghanem and W. Sabra (1996). Effect of nutrients on alginate synthesis in Azotobacter vinelandii and characterisation of the produced alginate. Microbiologia SEM 12: 593-598. W. Sabra (1999) Microaerophilic production of alginate by Azotobacter vinelandii. Dissertation, Technische Universität Carolo-Wilhelmina zu Braunschweig, Germany (Electronic publication address: http://www.biblio.tubs.de/ediss/data/19990415a/19990415a.html) W. Sabra; A.-P. Zeng; S. Sabry; S. Omar and W.-D. Deckwer. (1999). Effect of phosphate and oxygen concentrations on alginate production and stoichiometry of metabolism of Azotobacter vinelandii under microaerobic conditions. Appl. Microbiol. Biotechnol. 52: 773-780. W. Sabra; A.-P. Zeng; H. Lünsdorf and W.-D. Deckwer. (2000). Function and variation of alginate production in Azotobacter vinelandii. Appl. Environm. Microbiolgy 66(9): 4037-4044. W. Sabra; A.-P Zeng and W.-D. Deckwer. (2001). Bacterial alginate: physiology, product quality and process aspects. Appl. Microbiol Biotechnol. 56:315-325. W. Sabra; Kim Eun-Jin and A-P. Zeng (2002). Physiological responses of Pseudomonas aeruginosa PAO1 to oxidative stress in controlled microaerobic and aerobic cultures. Microbiology, 148: 3195-3202. W. Sabra, H. Ma, W. Wang, E.-J. Kim, A.-P. Zeng (2002). Rekonstruktion und Analyse des metabolischen Netzwerks von Pseudomonas aeruginosa für die Bildung von Virulenzfaktoren. Chemie Ingenieur Technik: 74, Issue: 5, Date: May, 2002, Pages: 688 W. Sabra; H. Lünsdorf and A-P. Zeng (2003). Alterations in the formation of lipopolysaccharide and membrane vesicles on the surface of Pseudomonas aeruginosa PAO1 under oxygen stress conditions. Microbiology, 149, 2789–2795. Kim E.-J., Sabra W. and A-P. Zeng (2003). Iron deficiency leads to blockage of oxygen transfer and enhanced formation of virulence factors in culture of Pseudomonas aeruginosa PAO1. Microbiology, 149, 2627–2634. Sabra , W. (2006). The increase in guluronic acid residues in alginate diminishes its specific formation rate (qalginate) in early growth phases of Azotobacter chroococcum. Assiut University J of Botany. 35(2): 423-436.ISSN: 1687-4927. Sabra W. and Hassan M. (2007) Exopolysaccharide yield as a kinetic parameter for the statistical optimization of EPS production by Klebsiella pneumoniae. Biotechnology 1-8 - - Sabra W. and Abou-Zeid Dunja (2008). Improving feeding strategies for maximizing Polyhdroxyalkanoates yield by Bacillus megaterium. Res J Microbiol 3(5) 308-318 Sabra, W. and A-P. Zeng (2008). Virulence and oxidative stress of Pseudomonas aeruginosa PAO1 in continuous culture, a proteomoic study. ( in preparation) Book contribution: - - W. Sabra; W.-D. Deckwer. (2004). Alginates - A polysaccharide of industrial interest and diverse biological functions. In: Polysaccharides, structural diversity and functional versatility, Second Edition. Ed. S. Dumitriu Chapter 21,pp 515533. Dumitriu, Marcel Dekker, New York. W. Sabra; A. Ross and R. Jonas (2004) Alginate versus PHB production by the diazotrophic growing culture of Azotobacter chroococcum. In “ Biotecnological advances and applications in bioconversion of renewable raw materials. 309-311, Editted by Jonas R., Pandey A. and Tharun G., Doeringdruck, ISBN: 3-925 26825-0 Conference poster and lecture: - - - - - W. Sabra; A.-P. Zeng and W.-D. Deckwer (1997). Effect of phosphate and oxygen concentrations on the respiratory quotient and alginate production by Azotobacter vinelandii: A stoichiometric study (Poster). 8th. European Congress on Biotechnology, Budapest, Hungary. W. Sabra; A.-P. Zeng and W.-D. Deckwer (1999). Kopplung von Nitrogenaseaktivität und Alginatproduktion in Azotobacter vinelandii. Lectures and- discussion conference 10-11Mai, DECHEMA e.V. Erfuhrt, Germany W. Sabra; A.-P. Zeng and W.-D. Deckwer (1999). ‘Biological Function and Variation of Alginate Production in Diazotrophically Growing Azotobacter vinelandii’. Biotechnology 2000 conference 3-8 September DECHEMA Berlin, Germany J-X. Bi, M. Wirth, C. Beer, W. Sabra, & A.-P. Zeng. (2001) The use of a CFOS-GFP reporter system for monitoring apoptosis of animal cells induced by exotoxins of Pseudomonas aeruginosa. Proceedings of the 17th Meeting of European Society for Animal Cell Technology, June 10-14, Tylösand, Sweden E. Kim, W. Sabra, A. Zeng (2003). Effect of iron on physiological change of Pseudomonas aeruginosa and virulence increment due to oxygen limitation phenomenon. DECHEMA, Section Bioprocess development 1.46, 2.4 April, Muenchen, Germany. Referee 1- Prof. Dr. An-Ping Zeng GBF (Germany see above), adjunct Professor at the universities of Dalian and Tianjin. Biochemical Engineering Division, Mascheroder Weg 1, D-38124 Braunschweig, Germany e-mail: AZE@gbf.de Fax: +49-531-6181 111 2- Prof. Dr. Rainer Jonas Head of Scientific Information and Library, Coordinator for International Co-operation with developing countries Mascheroder Weg 1, D-38124 Braunschweig, Germany e-mail: RJO@gbf.de Fax: +49-531-6181 575 or 515