Carp Breeding and Hatchery Management Indian major carp viz., catla (Catla catla), rohu, (Labeo rohita) and mrigal (Cirrhinus mrigala) and two exotic Chinese carps, silver carp (Hypophthalmichthys molitrix) and grass carp (Ctenopharyngodon idella) mature in confined water but fail to breed spontaneously in confinement. They are known to breed spontaneously during south-west monsoon in rivers. The first attempt to breed major carps in confined water was by bundh breeding technique. The use of pituitary hormone in carp breeding in India was established in 1957. It was on 10 th July, 1957, Cirrhinus reba, a medium carp induced to breed by administration of aqeous carp pituitary extract at Angul, Orissa by Scientists of Pond Culture Sub-station of Central Inland Fisheries Research Institute, Barrackpore, West Bengal. Within next fortnight, all three Indian major carps were induced bred successfully. The technique was further refined in subsequent years and has been widely practiced all over the country at farmers’ level. Besides pituitary extracts, in recent years several other formulations of hypothalamic peptide hormones are also used effectively for induced breeding of carps, which are commercially available in trade names of Ovaprim (Manufactured by Syndel Laboratory, Canada and marketed by Glaxo India Ltd.), Ovatide (Hemmo Pharma, Mumbai) and Wova-FH (Wokhardt, Mumbai). The confined water induced breeding was the hapa breeding system during sixties and seventies of 20th century. The system was open to environmental hazards, which lead to invariably low recovery of spawn. This could be overcome during eighties when eco-carp hatchery technology was introduced. Since then this has been subjected to several modifications for increasing the efficiency. Induced breeding Augmentation of prospective brood decides the success of induced breeding. Thus, maintenance of healthy brood in broodstock ponds is the prime requisite for successful breeding operation, which include stocking of adult fish @ 1500 kg/ha; provision of formulated diet @ 1-2% of the body weight daily; and water replenishment. In case of hypophysation, generally female carp receive pituitary extract in two split doses at an interval of 6 hrs. Male receives single dose at the time of 2nd dose to female. There are several ways of hormone administration to matured carp such as intracranial, intra-peritoneal and intramuscular. While intramuscular injection is given at the tail peduncle, intra-peritoneal injection is given just below the pectoral fin. The professional fish breeders prefer intra-peritoneal hormone administration procedure. Dose of the pituitary extract depends on species, brood quality and breeding environment. A dose of 3-6 mg/kg is administrated to the female followed by a second dose of 812 mg/kg after 6 hrs. Male receives 3-6 mg/kg, once at the time of second injection to female. Brood generally ovulates within 4-6 hrs of second dose. The development of hypothalamic hormone preparations has led to a more simpler and easy to use breeding practice in carps. Formulated induced agents have gained wide acceptance among fish seed producers mainly due to the following advantages: i) available are ready to use sperm, ii) less species specificity and iii) single dose administration for spawning, which reduces stress to fish. The doses of such hormonal preparations (Ovaprim/Ovatide/Wova-FH), in general, ranges 0.3 - 0.5 ml/kg body weight of female and 0.2 - 0.3 ml/kg body weight of males in all the three Indian major carps. Hatchery management Circular eco-hatchery is by far the most common hatchery systems adopted all over the country. The hatchery possesses three principal components viz., spawning pool, incubation/hatching chamber, and water storage and supply systems. Spawning pool is also called as breeding pool. Its purpose is to hold injected breeders for natural spawning and fertilization. The pool essentially needs a continuous supply of filtered well oxygenated running water of optimum temperature. The depth of water in the breeding pool is maintained up to 1.5 m based on the brood density and 3-5 kg of brood per m 3 is recommended. The breeding pool should have a sloping bottom leading to the outlet at centre, so that it can be completely drained when required without leaving any eggs behind. It is essential to View of an eco-hatchery provide a simulative riverine environment in spawning pool. The centrifugal flow makes the operation of inlet and outlet more effective. Automation has been incorporated to facilitate self transfer of fertilized eggs into hatching pools. The size and number of spawning pool may vary based on the production requirements and the size of the breeding pool. The duck mouths direction and the speed of water are well adjusted to assure favourable circulation of eggs in the water preventing them from mechanical injury. The circulation keeps the fertilized eggs evenly distributed in the water column and keeps them rolling slowly. 0.7- 0.8 eggs per cubic meter of waster is considered to be optimum for incubation. The circular pool has the Collection of spawn from hatching pool advantage of holding a large volume of eggs, convenient to manage and higher hatching of embryos which takes place within 14-18 hours in Indian major carps. The spawn are kept in the hatching pool till 72 hours before transfer to nursery. At present a large number of commercial eco-carp hatcheries are operating to produce the carp seed for culture in India. Intensification of carp seed production is not limited with the application of hatchery infrastructure. Now technology is available to breed the same fish 2-3 times in the season thereby resulting in 2-3 folds more production than the single breeding. Points to be taken care: Transport the brood by hamack (Canvas bags) filled with water to avoid stress Circulate the water in the pool about an hour before the ovulation period and continue till effective spawning (90-120 minutes) Don’t allow the spent brood to be in the pool after breeding for more time Brood fish should be used within the reproductive age group i.e., 2+ years to 5 years age Economics of carp seed production in eco-carp hatchery (Capacity, 50 million spawn) Sl. no. I. A. 1. 2. 3. 4. 5. Items Expenditure Fixed capital Bore well Overhead tank (capacity 20,000 l) Breeding pool 6 m diameter (1 no), Incubation chambers (1.5 m dia, 6 nos) & egg collection chamber (2.5 X 1 X 0.75 m, 1 no) and shed Intake and refuse water ponds (0.3 ha, 2 nos) Water pumps and accessories (Electric and diesel 5HP) Sub-total Amount (In rupees) 50,000 50,000 1,00,000 80,000 40,000 3,20,000 B. 1. 2. 3. 4. 5. Variable cost Prospective brood fish 1000 kg @Rs 50/kg Fuel for diesel pump, electricity, etc. Disinfectant, inducing agents, etc. 6 full time/part time workers @ Rs. 2000 per man-month Miscellaneous expenditure Sub-total C. 1. 2. 3. Total Costs Total variable costs Depreciation cost on fixed capital @ 10% yearly Interest on fixed capital @15% per annum Grand Total 1,31,000 32,000 48,000 2,11,000 II. Gross Income Sale of spent brood 1000 kg @ Rs. 40/kg Sale of spawn @ Rs 500/lakh Gross Return 40,000 2,25,000 2,65,000 Net Income (Gross income - Total costs) 54,000 III. 50,000 20,000 15,000 36,000 10,000 1,31,000