TC_lab_402_Induction_Supporting_Document

advertisement

TC lab 402 – PC1 Laboratory Induction Supporting Document

This Supporting Document should be used in conjunction with form OHS049 when given your initial Laboratory Induction.

On induction completion, include in the Laboratory Manual Induction tab.

Date: Trainer:

 Staff  Student Name: UNSW ID:

Extension no: Mobile: Email:

Access requirements – normal & after hours

(tick when completed)

Users should have swipe card access to Wallace Wurth building

Once induction is completed, users will be issued a lab key

First time users should be supervised

Normal working hours: 8:01 am – 5:59 pm Monday to Friday

After hours: 6pm – 8am Monday to Friday and all day Saturday and Sunday

Working after hours: risk assessment, competency of worker, approval to undertake the work

 Risk assessments

A risk assessment must be first completed before undertaking a new procedure

Completed risk assessment forms are filed in Lab Manual under the Risk Assessment tab

 Safe Working Procedures (SWP)

SWP should be written by staff/students with sound, hands-on experience and knowledge of the tasks

SWP are kept in the Lab Manual under the SWP tab

Biohazard class 2 hood use: bookings must be made via http://php.med.unsw.edu.au/somswiki/index.php?title=MedCells#BioHood_Booking

 Incubators: separate primary cultures from cell lines, human stem cells from animal cells

 Centrifuge: users to be trained/supervised

Fluorescent microscope (owned by SCL): users to be trained prior to use. Bookings must be made via http://php.med.unsw.edu.au/somswiki/index.php?title=MedCells#Microscope_Booking

Water bath: media/bottles must be sprayed with 70% ethanol and wipe dry prior to use. Water bath is cleaned every

2 weeks with SigmaClean solution (0.16

 l/L) added to RO water.

Walk-in (4C and -20C) cold room: groups will be allocated cold room space. Each group is responsible for obtaining their own storage boxes (clearly labelled) to house their individual items

Storage: each group will be allocated their own storage space with their items clearly labelled with the groups’ name

Labelling: anything that is used in the lab (eg. media bottles) must be clearly labelled with the user’s name, date, substance. If it is not labelled, it will be thrown out.

Rosters: each group will manage their own waste disposal and cleaning of their own hoods and incubators. Shared laundry and water bath cleaning will be rostered in a fortnightly basis.

Personal Protective Clothing and Equipment

No food, drinks, makeup application

 Hand wash and dry: wash hands before and after entering the lab

 All users must wear gloves, lab coat and closed footwear at all times

Protective safety goggles: spares ones in a drawer inside the lab

Emergency shower and eyewash

Laboratory Manual – location and use

 Lab manual includes the following tabs: induction, training, risk assessment, SWP, equipment servicing

 Located in corner shelf nearest to the fluorescent microscope/service door

Laboratory records including checklists and inspections

Each group is responsible for maintaining/servicing their own equipment

Page | 1

TC lab 402 – PC1 Laboratory Induction Supporting Document

Equipment servicing/maintenance records (divided by research groups) in Lab Manual folder

Chemical storage and register

Ethanol stored in flammables cabinet

Bleach

Other chemicals eg. DMSO

Material Safety Data Sheets (MSDS)

Person ordering a new chemical MUST provide an MSDS

Hard copies in the MSDS folder located in the corner shelf near the fluorescent microscope

If there is a digital version, save this in \\Ls-gl9e0\share\STEM CELL LAB\OHS\MSDS

Waste disposal system 

General waste (paper/packaging): green bins with black plastic lining

Sharps: sharps bin to be purchased from Webstores

Biowaste: white bins with biohazard bag. Serological pipettes to be placed in a separate biowaste bin

Chemical waste: bleach must be added to discarded cell media and placed in white plastic containers. Use UNSW waste forms for collection http://www.hr.unsw.edu.au/ohswc/ohs/ohs_waste.html

 Autoclave: drop off and pick up service in Lowy building, lower ground floor (separate induction required)

Laboratory Emergency Response Plan

Nearest First Aid Kit: level 4 near the lifts

First Aid officers:

 Spills

Heads of research groups sharing 402:

Research Group Head

Stem Cell Lab A/Prof Kuldip sidhu

Ext

53938

Mobile

Email k.sidhu@unsw.edu.au

Cell Biology Lab

Pharmacology

Neural Injury Research Unit

Neuropharmacology

Dr Mark Hill

Dr Angela Finch

Dr Ross Grant

A/Prof Laurence Wakelin

52477

Dr Catherine Gorrie

Dr Nicole Jones

Pain Laboratory

Telephone/Emergency contacts:

Dr Gila Moalem-Taylor m.hill@unsw.edu.au

Name Role Ext Mobile Email

Dr Sophia Dean SCL (on-site) lab manager (Tues-Thur) 52002 0414 337 871 s.dean@unsw.edu.au

Dr Mark Hill

Daniel Lie

Jenny Hartley

Cell Biology head

SCL lab contact (Mon & Fri)

SOMS OHS coordinator

52477

52002

58288

0404 797 480 m.hill@unsw.edu.au

khun.lie@unsw.edu.au

j.hartley@unsw.edu.au

Evacuation and emergency procedures

Review local OHS Web resources

 http://www.hr.unsw.edu.au/ohswc/ohs/ohs_home.html

Page | 2

Download