GEL DOCUMENTATION SYSTEM MANUFACTURER: SynGene MODEL: GeneGenius APPLICATION: The GeneGenius Imaging System from Syngene is principally designed for the detection and documentation of nucleic acids and proteins in gels. Nevertheless, here are described the main applications of this device in our laboratory: Documentation and image analysis of bands patterns or spot blots in gels, films or membranes (agarose or acrilamide gels dyed with etidium bromide, proteins dyed with coomasie or silver, TLC, x-ray, ELISA plates…) Colonies accounting. Densitometry. TECHNICAL SPECIFICATIONS: The system consists of a dark room, a UV transilluminator and a PC with a thermal printer. The darkroom is equipped with a CCD camera with a motor-operated zoom lens. The system also comes with the “one button” GeneTool analysis software that allows for the analysis of gels, arrays and colony plates. This GeneTool software allows users to count colonies on a plate and all data can be transferred into any type of spreadsheet program. The GeneGenius has a number of automatic functions: gel type detection, presentation of the gel image as a histogram and the creation of a table with the lanes numerically marked. More detailed information about the different components of the gel documentation system is here presented: CCD Video Camera with UV filter, zoom 12,5 – 75 mm. UV transilluminator (302 nm) with security dark camera. Digital card with GeneSnap software. GeneTools analysis software with automatic bands detection, background correction, quantity calibration, molecular mass, Rf … etc. Molecular mass markers library /histograms/ dendograms/ graphs.