Fukai Frank Bao CV
1. General Information
Professional Title:
Fukai Frank Bao, MD/PhD
1. Professor, and chairman, Department of
Microbiology，parasitology and Immunology
2. Director, The Institute for Tropical Medicine
3. CTO, Quality Control Station of Experimental Animals
Kunming Medical University, Kunming, China
4. Director, Pan-Asian Centers for Tropical Infectious Diseases
Contact Information:
Address：Department of Microbiology and Immunology,
Kunming Medical University
191 Renmingxi Road, Kunming 650031, China
Tel:
86－871－5314539
Fax:
86－871－5314539
Email:
baofukai@126.com，fukai.bao@yale.edu
Education:
1980~1985 MD, Kunming Medical College, Kunming, Yunnan, China
1985~1987 MS, Kunming Medical College, Kunming, Yunnan, China
1987~1990
PhD, Kunming Medical College, Kunming, Yunnan,
China
2002~2005 Postdoc, School of Medicine, Yale University, New Haven,
CT, USA
2009~2009 Visiting professor, Mahidol University and The Prince of
Songkla University, Thailand
2011-2011 Visiting professor, Mahidol University and The Prince of
Songkla University, Thailand
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Professional Membership:
Vice-President, International HUA-XIA Medical Society
Member，WHO Stop TB Partnership
Member, American Society for Microbiology (ASM)
Member, New York Academy of Sciences (NYAS)
Member, American Spirochete Research Community
Member, Chinese Society of Microbiology, Beijing, China
Committee member, Chinese Committee of Clinical Microbiology
Chairperson, Subcommittee of Intracellular Pathogens
Member, Chinese Society of Immunology, Beijing, China
Journal Editors And Reviewers:
Journal of Rapid Methods and Automation in Microbiology（Reviewer, Wiley）
Vector-Borne and Zoonotic Diseases（Reviewer, USA）
Journal of Entomology and Nematology ( Co-editor)
FEMS Immunology & Medical Microbiology (Reviewer)
Journal of Bacteriology Research（Associate editor）
Journal of Yeast and Fungus Research (co-editor)
The Journal of Infection in Developing Countries (JIDC,Italy)
Journal of Developmental Biology and Tissue Engineering ( Reviewer )
Clinical Reviews and Opinions（Member of editorial board）
Journal of Pharmacognosy and Phytotherapy(Reviewer)
Journal of Clinical Immunology and Immunopathology（Member of editorial
board）
China Tropical Medicine ( Editorial Board member and reviewer,China)
Chinese Journal of Medical and Pharmaceutical Sciences
( Editorial Board member and reviewer,China)
Chinese Journal of Clinical Laboratory Science(Reviewer, China)
Journal of Clinical Medicine and Research（reviewer）
Journal of Infectious Diseases and immunity（Member of editorial board）
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Journal of AIDS and HIV Research（reviewer）
Journal of Cancer Res and Exp Oncology（reviewer）
Journal Of Dentistry And Oral Hygiene（Reviewer）
Journal Of General And Molecular Virology（reviewer）
Journal of Developmental Biology and Tissue Engineering (Reviewer)
Progress in Physiological Sciences（Beijing, China）
Genetics and Developmental Biology（Beijing, China）
Life Sciences（Shanghai, China）
Chinese Journal of Epidemiology(（Member of editorial board, Beijing, China）
Research Opinions in Animal & Veterinary Sciences (ROAVS)（editorial board
member）
Journal of Pharmacognosy and Phytotheraphy
2. Lab’s Research Highlight:
(1) My laboratory is much interested in arthropod-borne pathogens
and its infections to the mammalian hosts.
Tropical
arthropod-borne
infectious
diseases
remain
an
major
international public health threat. My laboratory focuses on several kinds of
arthropod-transmitted diseases：
a. Borrelia burgdorferi, a species of the spirochete, which causes Lyme
disease.
b. Anaplasma, mainly Anaplasma phagocytophilum, an obligate
intracellular bacterium that persists within neutrophils, which causes human
granulocytic anaplasmosis and is primarily transmitted by Ixodes ticks.
c. Finally, richettsiaes, which are expanding their territory throughout the
world.
(2) We put hard efforts on Mycobacterium tuberculosis, tuberculosis
epidemiology, anti-tuberculosis natural products, TB immunology,
epidemiology, molecular typing, and pathogen-host interaction.
Tuberculosis(TB), An ancient disease, remains one of the major causes
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of disability and death worldwide. In 2006, 9.2 million new cases of TB
emerged and TB killed 1.7 million people. My lab is examining TB at its
fundamental levels through researches aimed at developing faster diagnostic
tools, better prevention and treatment strategies.
Tuberculosis, or TB, is an infectious bacterial disease caused by
Mycobacterium tuberculosis, which most commonly affects the lungs. It is
transmitted from person to person via droplets from the throat and lungs of
people with the active respiratory disease. In healthy people, infection with
Mycobacterium tuberculosis often causes no symptoms, since the person's
immune system acts to “wall off” the bacteria. The symptoms of active TB of
the lung are coughing, sometimes with sputum or blood, chest pains, weakness,
weight loss, fever and night sweats. Tuberculosis is treatable with a six-month
course of antibiotics (DOTS). A single sneeze can release up to 40,000
droplets.
Each one of these droplets may transmit the disease, since the
infectious dose of tuberculosis is very low and the inhalation of just a single
bacterium can cause a new infection.
About 90% of those infected with Mycobacterium tuberculosis have
asymptomatic, latent TB infection (sometimes called LTBI), with only a 10%
lifetime chance that a latent infection will progress to TB disease. However, if
untreated, the death rate for these active TB cases is more than 50%. TB
infection begins when the mycobacteria reach the pulmonary alveoli, where
they invade and replicate within the endosomes of alveolar macrophages. The
primary site of infection in the lungs is called the Ghon focus, and is generally
located in either the upper part of the lower lobe, or the lower part of the upper
lobe. Bacteria are picked up by dendritic cells, which do not allow replication,
although these cells can transport the bacilli to local (mediastinal) lymph
nodes. All parts of the body can be affected by the disease, though it rarely
affects the heart, skeletal muscles, pancreas and thyroid.
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Tuberculosis is classified as one of the granulomatous inflammatory
conditions. Macrophages, T lymphocytes, B lymphocytes and fibroblasts are
among the cells that aggregate to form a granuloma, with lymphocytes
surrounding the infected macrophages. The granuloma functions not only
prevent dissemination of the mycobacteria,
but also provides a local
environment for communication of cells of the immune system. Within the
granuloma, T lymphocytes secrete cytokines such as interferon gamma, which
activates macrophages to destroy the bacteria with which they are infected.
Cytotoxic T cells can also directly kill infected cells, by secreting perforin and
granulysin.
Importantly, bacteria are not always eliminated within the granuloma,
but can become dormant, resulting in a latent infection. Another feature of the
granulomas of human tuberculosis is the development of cell death, also called
necrosis, in the center of tubercles. this necrosis has the texture of soft white
cheese and was termed caseous necrosis.
(3) Our research is also focused on Molecular epidemiology and ecology of
tropical vector-borne pathogen infections, and characterizing tropical
vector-borne pathogens, particularly tick-transmitted rickettsial disease
agents, including Ehrlichia chaffeensis，Anaplasma phagocytophilum and
mite-transmitted Orientia, and also mosquito-transmitted virus infection,
such as dengue virus, Chikungunya virus.
We use the tools of field epidemiology, molecular biology, immunology,
animal models and cell culture systems. E. chaffeensis is the agent causing
human monocytic ehrlichiosis (HME). It also infects dogs, white-tailed deer,
goats and coyotes. These closely related pathogens remain in both arthropod
and vertebrate hosts for long periods of time. This strategy may aid them in
their parasitic lifestyle. Determining the molecular basis for persistence by
these bacteria of the genus Ehrlichia will be important in order to accomplish
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our long range goals of developing effective methods of control against these
tick-borne pathogens. Our research is focused on understanding the pathogen
evasion mechanisms. Specifically, we study to define the protective
host-immune response needed to eliminate the infection. We also study to
define how these rickettsiales evade host responses in both vertebrate and tick
hosts in order to support their life cycle.
3. Lab Facilities and Equipments:
Our well-equipped lab system includes 1 high-quality immunology/cell culture
room (80m2), 1 biosafety level-2 microbiology room (80m2), and 2 biosafety
level-3 labs (200m2). The lab system has been well equipped with biosafety
cabinets, ultra-clean hoods, ultra-speed frozen centrifuges, ultra-low freezers,
Gel Image - Forming System, PCR machines, DNA-RNA-protein
electrophoresis systems, Western blot machine, fluorescence microscope,
ELISA reader, CO2 cell incubator, visible-UV spectrophotometers, common
incubators, and other basic research tools.
4. Experimental Technology and Skills Platfrom:
Our lab system has strong abilities for technical services,including:
1. In vivo and in vitro activity screening of bioactive products
and natural products against bacteria,fungi,infectious
diseases, and tuberculosis
2. In vivo and in vitro activity screening for immuno-regulation
activities of
bioactive products and natural products
3. Systematic classification and identification of new bacteria
and fungi; Isolation and identification of bacteria and
fungi in various samples
4. Emergency response and consultation of sudden tropical
infectious diseases
5. PCR, RT-PCR, Western blotting, ELISA techniques
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6. Extraction and purification of DNA, RNA, and protein
7. Cell culture skills, and animal experiment skills
5. Lab Members:
Our lab hosts a strong active multi-disciplinary group of 30 members,
their research fields are involved in microbiology, immunology, molecular
biology, field epidemiology, infectious diseases, vector biology, and
bioinformatics, etc. We also recruit graduate students and short-time training
foreign students every year.
6. Collaboration and Information Communication:
1. WHO Stop TB Partnership，Geneva
2. TB Communities, Global Health Delivery Project
3. Southeastern National Tuberculosis Center，
University of Florida, USA
The University of Southern Mississippi,USA
Yale University School of Medicine,USA
5. McGill University, Canada
6. Prince of Songkla Unversity, Thailand
MahidolUniversity, Thailand
7. National University of Moscow, Russia
8. The Institute of Tropical Medicine, Nagasaki University,
Nagasaki, Japan
9.Ditan Hospital, Beijing, China
People’s Hospital, Beijing, China
The Institute for Pathogen Biology, Chinese Academy of Mdecine,
Beijing, China
CDC,Chengdu Military Region, Chengdu, China
The Yunnan Institute of Endemic Disease prevention and Control,
Dali, Yunnan, China
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The Third Hospital of Kunming (Kunming Hospital for Infectious
Diseases, Kunming, Yunnan, China
7. Recent Main Publications(2004~)：
１） Pal U, Wang P, Bao F(First authors with equal contribution),
Yang X, Samanta S, Robert Schoen , Wormser GP, Schwartz I, and
Erol Fikrig. Borrelia burgdorferi basic membrane proteins A and
B participate in the genesis of Lyme arthritis. J Exp Med.
2008; 205: 133-141.(SCI Impact factor：15.6, 2008)
２） Utpal Pal, Xin Li, Tian Wang, Ruth R. Montgomery, Nandhini
Ramamoorthi, Aravinda M. deSilva, Fukai Bao, Xiaofeng Yang,
Marc Pypaert, Deepti Pradhan, Fred S.
Kantor, Sam Telford,
John F. Anderson, and Erol Fikrig. TROSPA, an Ixodes
scapularis receptor for Borrelia burgdorferi. Cell.2004；
119:457-468.（SCI Impact factor：32.４）
３） Nandhini Ramamoorthi, Sukanya Narasimhan , Utpal Pal, Fukai
Bao, Xiaofeng F. Yang ,Juan Anguita, Michael V. Norgard, Fred
S. Kantor, John F. Anderson, Raymond A. Koski and Erol Fikrig.
The Lyme disease agent exploits a tick protein to infect the
mammalian host.Nature.2005;436:573-577.（SCI Impact factor：
32.7）
４）Yongli Zhang, Fukai Bao, Jianjian Hu, et al. Antimicrobial lignans and
triterpenoids from Rostellularia prcumbens. Planta Medica. 2007;
73:1596-1599. (SCI journals)
５）Hengxing Liang, Fukai Bao, Xiaoping Dong , Rui Tan , Caijun Zhang ,
Qing Lu
and Yongxian Cheng. Antibacterial Thymol Derivatives
Isolated from Centipeda minima.
Molecules. 2007; 12:1606-1613.
（SCI jurnal）
６）Heng-Xing Liang, Fu-Kai Bao, Xiao-Ping Dong, Hua-Jie Zhu, Xiao-Jie
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Lua, Ming Shi, Qing Lu, and Yong-Xian Cheng. Two New Antibacterial
Sesquiterpenoids from Centipeda minima. Chemistry &
Biodiversity.
2007; 4:2810-2816. （SCI journal）
７）Ren-Qiang Mei , Qing Lu, Yan-Fen Hu, Hai-Yang Liu, Fu-Kai Bao, Yu
Zhang, Yong-Xian Cheng. Three New Polyyne (=Polyacetylene)
Glucosides from the Edible Roots of Codonopsis cordifolioidea.
Helvetica Chimica Acta. 2008; 91:90 – 96.（SCIjournal）
８）Fengwei Bai, Tian Wang, Utpal Pal, Fukai Bao, L. Hannah Gould and
Erol Fikrig. Prevention of lethal murine West Nile virus infection using
RNA interference.
Journal of Infectious Diseases. 191:1148-1154,
2005. (SCI journal)
９) Fukai Bao, Erol Fikrig. Experimental research on the coinfection with
Borrelia burgdorferi and Anaplasma phagocytophilum. Journal of
Tropical Medicine，2007；７：195-200.
10)
Fukai Bao, Erol Fikrig. Coinfection with Borrelia burgdorferi and
Anaplasma phagocytophilum inhibits the production of Th1 cytokines.
China Tropical Medicine. 2007；７：1061-1063.
11)
Fukai Bao. Research advances in the coinfection of tick-borne
pathogens。China Tropical Medicine. 2007，7（1）：112～114
12）Fukai Bao. Research progress in Borrelia burgdorferi and Lyme disease.
Journal of Tropical
Medicine. 2007；7:1125-1127
13）Fukai Bao. Systematic taxonomy of Rickettsiales. Chinese Journal of
Zoonoses. 2007;23:1262-1264
14） Fukai Bao, Aihua Liu, Yu Zhang。In Vivo Anti-Tubeculous Effect
of Tiankejun Capsule。Journal of Tropical Medicine，2006，6（3）：
975-977
15）Aihua Liu,Fukai Bao*,Jie Zhang, Jue Shu。Observation of the antibacterial
effect of extracts from Herba Euphorbia humifusa on oral pathogenic
bacteria in vitro. China Tropical Medicine. 2007 ，7 （12 ）：2194,2217
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16) Aihua Liu，Bixuan Li，Min Shi，Fukai Bao*. Terpenoids YK—1 and
YK—2 shows anti-bacterial activities in vitro. Chinese Journal of
Pathogen Biology. 2008, 3（3）：176-177
17 ） Henxin Liang, Fuaki Bao, Xiaopin Dong, Qing Lu, Yongxian
Chen*.Antibacterial Triterpenes from Centipeda minima.Yunnan Plant
research. 2007 , 29 (4) : 479～482
18) Fukai Bao，Erol Fikrig. The pathogen-vector interactions during Borrelia
burgdorferi transmission. China Journal of Vector Biology and control。
2008, 19（3）：264－268
19)
Fukai Bao, Erol Fikrig. The Joint-specific Expression Profile of
Borrelia burgdorfri in the Murine Hosts.
Science and Technology
Bulletin. 2008, 24（6）:832-838
20) Fukai Bao*，Aihua Liu，Haibin Ma. Advances in pathogen biology and
epidemiology of human anaplasmiosis. Journal of Tropical Medicine，
2008，8（11）：1193-1195
21) Fukai Bao*，Aihua Liu，Haibin Ma，Na Wu, Min Shi. Advances in the
pathogenesis of Lyme arthritis. Chinese Journal of Pathogen Biology.
2009,4（5）：380～382
22) Aihua Liu，Lin Chen，Caijun Zhang，Fang Chen，Min Shi，Liang Chen，
Peiqing Shen，Ruwen Liu，Jianlin Jiao，Malin Li，Fukai Bao*.Touchdown
PCR detected Borrelia burgdorferi in wild tree shrew. 2009，9（4）：621～
622
23) Fukai Bao*，Aihua Liu。Quantitative research of Borrelia burgdorferi
burden in the murine model。Chinese Vector Biology and control。2009,
20（3）：234～236
24 ） Aihua
Liu ， Fukai
Bao* 。 Newly-found
inhibitory
cytokine—Interleukin(IL)-35。Chinese Journal Of Immunology. 2009; 25
25）Li
Li，Fukai Bao*。Il-32 and its relations to inflammatiory diseases.
Journal of Current Immunology. 2009，25：284～285
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26）Fukai Bao*, Aihua Liu, Min Shi，Haibin Ma。Advances in Clinical
symptoms, diagnosis, and treatment of human anaplasmiosis. Journal of
Tropical Nedicine。2009；9: In press
27 ） Liang Chen ， Fukai Bao*.
Isoniazid-resistant genes and resistant
mechanisms of Mycobacterium tuberculosis. Chinese Journal of
Zoonoses. 2009;25（3）:288～291
28 ） Fukai Bao* ， Aihua liu ， Chuanxian Chen 。 Epidemic trend,
cause-consequence analyses,and control strategies. China Tropical
Medicine. 2009 ，9（3）：599～562
29）Aihua Liu，Fukai Bao*。Some important advances in innate lmmunity
study. Chinese Journal of Nature. 2009；31（4）; 218～222
30）Ai-hua Liu, Min Shi, Cai-jun Zhang, Xiao-jie Li, Xin Wang, Pei-qing
Shen，Ma-lin Li , Fukai Bao*. . Domesticated tree shrew ( Tupaia
belangeri ) could be infected with Serratia grimesii. Afr J Biotech. 2010;
(SCI journal)
31) Qun XUAN， Fukai BAO*.， Hongmei PAN，Aihua LIU 3*. Isolating
Fungal Endophyte from Paris polyphylla Smith var. yunnanensis and
Identifying Their Antibacterial Ability. Afr J Microbiol Res. 2010; (SCI
journal)
32) BinXue Li, Fukai BAO*, Aihua LIU. Research progress on
relationship between tumour necrosis factor-ɑ and tuberculosis.
China Tropical Medicine. 2010;10(3): 370-372
33) ZHANG Caijun , SHI Ming , CHEN Fang , CHEN Liang , SHEN Peiqing,
BAO Fukai*, L I Malin*.
Isolation and identification of common
bacteria in tree shrews. China Journal of Pathogen Biolog y. 2009;4(12):
899-900
34) BAO Fukai*, L IU Aihua. Progress in the study of Ehrlichia cha f f eensis
and human monocytic ehrl ichiosis. China Journal of Pathogen Biology.
2009;4(12): 935-938
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35) Dong Shasha，Bao Fukai，Lu Qing，Chneg Yongxian1*. GC-MS Analysis
of the Chemical Constituents of Essential Oil from Hibiscus sabdariffa
Linn. and Study on Antibacterial Activity. Journal of Dali University. 2009;
8(6):1-4
36) LIU Aihua, XUAN Qun, BAO Fukai. Progress of research on IL-32 and
its
relationship
with
tuberculosis.
China
Journal
of
Pathogen
Biology.2010；5(3):215-217
37)
( * correspondence author)
B. Scientific Books
1.
Infection and Microbes (900 pages) Chendu: Chendu University of
Science and Technology Publishing House, 1995. (As the first vice
editor-in-chief)
2.
Medical Microbiology (400 pages, textbook for college student).
Shanghai: Shanghai Scientific and Technology Press, 1995. (As one of
authors)
3.
Abbreviations and Terms in Immunology (324 pages). Peking: Academic
Press, 2002. (As one of authors)
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