Next Gen DNA Sequencing Services Inquiry (Please fill out all fields where applicable, if you
are unsure of an answer just put “do not know” and we will assist you in determining the answer.)
Upon completion please send as an attachment to nextgen@genome.med.harvard.edu
Your Name (Last, First):
BPF User Account*
Email:
Phone:
PI Name (Last, First):
Department:
Institute:
*If you do not have a BPF account, please sign up at www.genome.med.harvard.edu
Type of Sample Prep / Analysis: (Please complete a separate sheet for each type)
Genomic Re-Sequencing
ChIP-Sequencing
Digital Gene Expression NlaIII / Dpnll
Whole Transcriptome Analysis
Small RNA (microRNA) Analysis
Custom Tag Counting
Other
Sample Information:
1. Organism:
2. Source Material:
3. Number of Samples:
4. I have access to a reference genome or transcript database.
5. Organism’s genome size (mbs):
Recommended material per sample
Genomic Re-Sequencing
1 - 5 μg genomic DNA
DGE Gene Expression
1 - 2 μg Total RNA
Small RNA
10 μg Total RNA
ChIP-Seq
10 ng ChIP enriched DNA
in 50 μl TE
in 50 μl water
in 10 μl water
in30 ul water
6. Amount of material available per sample (μg):
7. Level of coverage you hope to obtain (eg. 20x or 4 million tags):
8. Sample Population is:
Single
Mixed
If applicable:
9. Size distribution of the IP material:
10. Size distribution of the RNAs searching for:
11. Types of mutations you are looking for:
12. Please provide any other information you feel would be helpful to us or a brief description of
your customized project with as much detail as possible to help us determine feasibility.
Data Analysis:
At this time we are offering a limited amount of data analysis assistance. We will discuss your
data analysis needs in further detail at the time of consultation.
Data Distribution:
Harvard Labs
I have an ecommons account and would like to use the BPF's storage on the HMS
Storage Array for a small monthly fee.
I have storage available and would like to retrieve my files via ftp.
I will provide a portable hard drive to obtain my files.
I would like to lease a portable hard drive from the BPF to obtain my files.
I am unsure and would like to discuss this further.
Non-Harvard Labs
I have storage available and would like to retrieve my files via ftp.
I will provide a portable hard drive to obtain my files.
I would like to lease a portable hard drive from the BPF to obtain my files.
I am unsure and would like to discuss this further.
Please email this completed word document as an attachment to
nextgen@genome.med.harvard.edu. We will send an acknowledgement as soon as we receive
your document. You will be contacted to set up further consultations upon our review of your
project. During the consultation you will recieve final project specifications and a formal quote for
all services. A purchase order must be submitted to secure a position in the Genome Analyzer
queue.