1
Supplementary Table 1. Echocardiographic function of cell-treated vs.
saline-treated infarcted hearts at 2 weeks and 6 weeks post-injury.
Echocardiographic measurements were made at both the basal (mid-papillary)
level and at the apical level. Sample sizes were 11 for cell treatment and 7 for
saline treatment. Comparisons were made between cell-treated and control
groups using two-tailed Student’s t-test and significant differences (p<0.05) are
marked with an *. Abbreviations: EDD, left ventricular end-diastolic diameter;
ESD, left ventricular end-systolic diameter; % FS, percent fractional shortening,
defined as (EDD-ESD)/EDD.
Parameter
Location
Treatment Group
Mean at 2 weeks
Mean at 6 weeks
EDD
Basal
Cell-treated
3.78 ± 0.65 mm
4.05 ± 0.44 mm*
EDD
Basal
Saline-treated
4.24 ± 0.76 mm
4.83 ± 1.14 mm*
ESD
Basal
Cell-treated
3.01 ± 0.86 mm
3.21 ± 0.65 mm*
ESD
Basal
Saline-treated
3.38 ± 0.97 mm
4.23 ± 1.31 mm*
% FS
Basal
Cell-treated
21.70 ± 11.92
21.19 ± 9.43
% FS
Basal
Saline-treated
21.24 ± 8.24
13.08 ± 7.28
EDD
Apical
Cell-treated
2.60 ± 0.45 mm
2.17 ± 0.20 mm*
EDD
Apical
Saline-treated
3.03 ± 1.27 mm
3.01 ± 0.74 mm*
ESD
Apical
Cell-treated
2.12 ± 0.41 mm
1.63 ± 0.34 mm*
ESD
Apical
Saline-treated
2.37 ± 0.89 mm
2.64 ± 0.88 mm*
% FS
Apical
Cell-treated
15.67 ± 6.80
25.75 ± 10.50*
% FS
Apical
Saline-treated
19.18 ± 13.27
13.87 ± 7.83 *
2
Supplementary Table 2. Hemodynamic parameters of cell-treated and salinetreated infarcted animals 6 weeks after infarction. Comparison is made to
sham-operated animals 6 weeks after thoracotomy. Units for SBP, DBP, MAP,
and EDLVP are mm Hg. Units for max and min dP/dt are mm Hg s-1. Pairwise
comparisons were made using the Student’s t-test with Bonferroni correction for
multiple comparisons. There are statistically significant differences between
EDLVP, max dP/dt, and min dP/dt between sham-operated animals and
infarcted animals (cell-treated or saline treated).
Group
HR
SBP
DBP
MAP
EDLVP
max dP/dt
min dP/dt
Cell-treated
427.8  52.4
86.0  9.8
59.5  4.7
67.5  5.4
7.9  7.1
3735.8  774.7
-3050.0  666.0
Saline
393.5  36.3
88.5  10.3
63.3  6.6
70.8  7.5
7.5  7.3
3240.8  623.6
-2704.8  605.3
Sham
403.1  33.2
94.3 7.2
61.3  6.1
71.2  6.2
0.1  1.9
4462.3  341.9
-4111.0  476.7
3
Supplementary Table 3. Hematopoietic chimerism in parabiotic pairs.
Nontransgenic (nonTg) mice underwent coronary artery ligation and were
surgically joined to GFP transgenic partners for 8 weeks. Hematopoietic cell
chimerism in the blood, spleen, and BM of nontransgenic partners (% GFP +
cells) was determined by flow cytometry. Chimerism of BM LT-HSC was
determined after staining for c-kit, Thy1.1, lineage markers, and Sca-1.
Abbreviations: BM, bone marrow; LT-HSC, long-term reconstituting
hematopoietic stem cell.
Pair #
Blood
Spleen
BM
BM LT-HSC
1
39.6
42.4
10.0
7.4
2
44.4
36.8
5.1
3.4
3
49.6
38.2
7.1
5.7
4
40.3
34.8
4.7
5.7
Average ± SD
43.5 ± 4.6
38.1 ± 3.2
6.7 ± 2.4
5.6 ± 1.6